Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000931-TA|BGIBMGA000931-PA|IPR006941|Ribonuclease CAF1,
IPR012337|Polynucleotidyl transferase, Ribonuclease H fold
         (318 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC18.06c |caf1|pop2|CCR4-Not complex subunit Caf1|Schizosaccha...   297   1e-81
SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces po...    42   2e-04
SPBC776.06c |||spindle pole body interacting protein |Schizosacc...    27   4.7  
SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9 |Sch...    26   6.1  
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces...    26   6.1  
SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1||...    26   8.1  

>SPCC18.06c |caf1|pop2|CCR4-Not complex subunit
           Caf1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 332

 Score =  297 bits (729), Expect = 1e-81
 Identities = 138/260 (53%), Positives = 182/260 (70%), Gaps = 1/260 (0%)

Query: 49  IKDVWNHNLHEEFQIIRQVVQKYHWVAMDTEFPGVVARPIGEFRSTADYQYQLLRCNVDL 108
           I+DVW+ NL +E  +I  ++++Y  V+MDTEFPGVVARP+G F+S+ DY YQ LR NVD 
Sbjct: 22  IRDVWSTNLQQEMNLIMSLIERYPVVSMDTEFPGVVARPLGVFKSSDDYHYQTLRANVDS 81

Query: 109 LRIIQLGLTFMDENGKTPPGYTTWQFNFKFNLQEDMYAQDSIDLLQNSGLQFRKHEEDGI 168
           L+IIQ+GL   DE G  P    TWQFNF FNLQ+DMYA +SI+LL  SG+ F+KH+E GI
Sbjct: 82  LKIIQIGLALSDEEGNAPVEACTWQFNFTFNLQDDMYAPESIELLTKSGIDFKKHQEVGI 141

Query: 169 EPLEFAELLMSSGLVLIDNIKWLSFHSGYDFGYLLKLLTDQNLPQDENDFFESLRLYFPT 228
           EP +FAELL+ SGLVL + + W++FHSGYDF YLLK +T   LP +  +F++ L +YFP 
Sbjct: 142 EPADFAELLIGSGLVLQEEVTWITFHSGYDFAYLLKAMTQIPLPAEYEEFYKILCIYFPK 201

Query: 229 VYDVKYLMKLCKNLKGGLQEVADQLELRRVGPQHQAGSDSHLTGMAXXXXXXXXXDDNIE 288
            YD+KY+MK   N   GLQ++AD L++ R+GPQHQAGSD+ LT            D +I+
Sbjct: 202 NYDIKYIMKSVLNNSKGLQDIADDLQIHRIGPQHQAGSDALLTARIFFEIRSRYFDGSID 261

Query: 289 SSS-GHLYGLGAPFSVNVNN 307
           S     LYGLG+  SV  +N
Sbjct: 262 SRMLNQLYGLGSTGSVLWHN 281


>SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 427

 Score = 41.5 bits (93), Expect = 2e-04
 Identities = 34/132 (25%), Positives = 61/132 (46%), Gaps = 7/132 (5%)

Query: 51  DVWNHNLHEEFQIIRQVVQKYHWVAMDTEFPGVVARPIGEFRSTADYQYQLLRCNVDLLR 110
           ++   N  E  + + + V   H+V++D EF G++       ++T   +Y+LLR +     
Sbjct: 2   EIHGKNFLETLKELEKHVDSAHYVSIDCEFSGLLRDFNLNNKNTLQDRYELLRKSSIRYT 61

Query: 111 IIQLGLTF--MDENGKT---PPGYTTWQF-NFKFNLQEDMYAQ-DSIDLLQNSGLQFRKH 163
           I+Q+G+TF  +  NGK+   P           + +L+ D  ++  SI  L   G  F K 
Sbjct: 62  ILQIGITFIYLQNNGKSSCIPVNINVSPLVKDELHLKRDFCSEASSIKFLIQQGFDFNKQ 121

Query: 164 EEDGIEPLEFAE 175
             +G+  L   E
Sbjct: 122 LTEGVPYLSRIE 133


>SPBC776.06c |||spindle pole body interacting protein
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 598

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 1/54 (1%)

Query: 198 DFGYLLKLLTDQNLPQ-DENDFFESLRLYFPTVYDVKYLMKLCKNLKGGLQEVA 250
           D  + L  L + +L   D  + F +L  +  T  DV YL+  C    GGL  +A
Sbjct: 513 DIQFHLDRLRNSSLSTTDAAEIFLALESHIRTEEDVNYLLSNCPLHSGGLSVIA 566


>SPAC1834.05 |alg9||mannosyltransferase complex subunit Alg9
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 577

 Score = 26.2 bits (55), Expect = 6.1
 Identities = 14/24 (58%), Positives = 15/24 (62%)

Query: 152 LLQNSGLQFRKHEEDGIEPLEFAE 175
           L  NS L+F K E DGI P EF E
Sbjct: 437 LPDNSRLKFVKSEFDGILPGEFVE 460


>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 662

 Score = 26.2 bits (55), Expect = 6.1
 Identities = 16/56 (28%), Positives = 26/56 (46%), Gaps = 1/56 (1%)

Query: 117 TFMDENGKTPPGYTT-WQFNFKFNLQEDMYAQDSIDLLQNSGLQFRKHEEDGIEPL 171
           T + E+    P Y+  W    +  L +   A  +I LLQ +G +F KH+   +  L
Sbjct: 336 TLVFESTPAYPDYSRYWSVVERHRLTQWYIAPTAIRLLQRAGNEFVKHDRSSLRVL 391


>SPAC1565.05 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 773

 Score = 25.8 bits (54), Expect = 8.1
 Identities = 10/33 (30%), Positives = 20/33 (60%)

Query: 54  NHNLHEEFQIIRQVVQKYHWVAMDTEFPGVVAR 86
           +H+L +E   +R + ++ + +  D +F GVV R
Sbjct: 450 DHSLRDELISLRSLAEQKNTIEFDAKFLGVVER 482


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.319    0.137    0.412 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,532,355
Number of Sequences: 5004
Number of extensions: 64836
Number of successful extensions: 154
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 149
Number of HSP's gapped (non-prelim): 6
length of query: 318
length of database: 2,362,478
effective HSP length: 73
effective length of query: 245
effective length of database: 1,997,186
effective search space: 489310570
effective search space used: 489310570
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 54 (25.8 bits)