BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000927-TA|BGIBMGA000927- PA|IPR000175|Sodium:neurotransmitter symporter (632 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AY395071-1|AAQ96727.1| 646|Apis mellifera GABA neurotransmitter... 460 e-131 AY395072-1|AAQ96728.1| 593|Apis mellifera GABA neurotransmitter... 460 e-131 AY395073-1|AAQ96729.1| 203|Apis mellifera GABA neurotransmitter... 173 3e-45 >AY395071-1|AAQ96727.1| 646|Apis mellifera GABA neurotransmitter transporter-1B protein. Length = 646 Score = 460 bits (1135), Expect = e-131 Identities = 226/535 (42%), Positives = 317/535 (59%), Gaps = 22/535 (4%) Query: 24 PERGNWTGRFDFXXXXXXXXXXXXNVWRFPYLCYNNGGGAFLIPFTVMLVIAGLPLMFME 83 P+RG W+ + +F N+WRFPYLCY NGGGAF++P+ + L +AG+P+ ME Sbjct: 70 PDRGMWSSKIEFILSVVGLAIGLGNLWRFPYLCYKNGGGAFMVPYFIALALAGVPMFLME 129 Query: 84 LSFGQYAALGPVAVYNKFCPLFRGLGYGMVIVSSIVMLYYNLIIAWTIFYMTVSFSSIFY 143 LS GQ +G + V+ K P+F+G+GY ++S +YY +I+AW +FY+ VS Sbjct: 130 LSLGQMMTIGGLGVF-KIAPIFKGIGYATCVLSCWTNIYYIIILAWALFYLLVSLR---I 185 Query: 144 QLPWQNCKAEWSTKHCYSYEEADE--CEAQNGTYYLRQCFNQSYSVSHNITALAKTALRR 201 LPW+ C W+T++C + E E C Q+ C ++SH AL + Sbjct: 186 DLPWRTCGNPWNTRYCLTPTERLEALCWTQDEDII---CSTPIGNLSH--------ALLK 234 Query: 202 PPAEEYFTNQVLGLSSGIEETGEIRLGMAMCLLAAWVIVFLCLCKGVQSSGKVVYFTALF 261 P +E++ + L +S GIE G IR +A L W++ + C+ KGV+ +GKVVYFT+LF Sbjct: 235 DPVKEFWERRTLQISDGIENIGSIRWELAGTLAVVWIMCYFCIWKGVKWTGKVVYFTSLF 294 Query: 262 PYVVLVILFFRGVTLPGAITGILFYLTPDFSQLANAQVWGDAAVQIFFALSPAWGGLITL 321 PY +L IL RG+TLPGA+ G+ +Y+TP+ S+L++ +VW DA QIFF+ + G L+ L Sbjct: 295 PYALLTILLIRGLTLPGAMEGLKYYVTPNLSKLSDPEVWIDAVTQIFFSYALGLGALVAL 354 Query: 322 SSYNKFSNNCYIDSLIVAVSNIATSFFAGLVIFSVIGFLAHELNVSVDRVVDQGAGLAFI 381 SYNKF+NN Y D+LIV N TS +G+VIFSV+GF+AHE V V G GLAF+ Sbjct: 355 GSYNKFNNNVYKDALIVCTVNSCTSMLSGIVIFSVVGFMAHEQQKPVADVAASGPGLAFL 414 Query: 382 VYPEVVTRLPISPLWSILFFVMLLTLGLDSQFALMETVTTAILDRFPN-FRQKKGWVVFI 440 VYP V LP S +WS LFF ML+ +GLDSQF +E TA +D +P R++K + I Sbjct: 415 VYPSAVLELPGSSIWSCLFFFMLILIGLDSQFCTVEGFITAAVDEWPRLLRKRKELFIAI 474 Query: 441 VAVFGYLGGLIFTTNSGMYWLQLMDKYA-ANWSVLIIAIGECILIAWIYGAEKFCGDIQR 499 V YL GL T GMY QL+D YA + + +L + ECI I+W +G +F I+ Sbjct: 475 VCFVSYLIGLFCITEGGMYVFQLLDSYAVSGFCLLFLMFFECIAISWAFGVNRFYDGIRD 534 Query: 500 MIGRQSKLWVYLWSAMWRIITPAALVFILVFNWIEYKPASYGHYVYPMWADAVGW 554 MIG W W W I TPA V + FN I++ P Y Y YP W+ +GW Sbjct: 535 MIGYYPCCW---WKICWTITTPAICVGVFTFNIIKFVPVKYLTYEYPWWSHVLGW 586 >AY395072-1|AAQ96728.1| 593|Apis mellifera GABA neurotransmitter transporter-1B protein. Length = 593 Score = 460 bits (1134), Expect = e-131 Identities = 226/535 (42%), Positives = 317/535 (59%), Gaps = 22/535 (4%) Query: 24 PERGNWTGRFDFXXXXXXXXXXXXNVWRFPYLCYNNGGGAFLIPFTVMLVIAGLPLMFME 83 P+RG W+ + +F N+WRFPYLCY NGGGAF++P+ + L +AG+P+ ME Sbjct: 17 PDRGMWSSKIEFILSVVGLAIGLGNLWRFPYLCYKNGGGAFMVPYFIALALAGVPMFLME 76 Query: 84 LSFGQYAALGPVAVYNKFCPLFRGLGYGMVIVSSIVMLYYNLIIAWTIFYMTVSFSSIFY 143 LS GQ +G + V+ K P+F+G+GY ++S +YY +I+AW +FY+ VS Sbjct: 77 LSLGQMMTIGGLGVF-KIAPIFKGIGYATCVLSCWTNIYYIIILAWALFYLLVSLR---I 132 Query: 144 QLPWQNCKAEWSTKHCYSYEEADE--CEAQNGTYYLRQCFNQSYSVSHNITALAKTALRR 201 LPW+ C W+T++C + E E C Q+ C ++SH AL + Sbjct: 133 DLPWRTCGNPWNTRYCLTPTERLEALCWTQDEDVI---CSTPIGNLSH--------ALLK 181 Query: 202 PPAEEYFTNQVLGLSSGIEETGEIRLGMAMCLLAAWVIVFLCLCKGVQSSGKVVYFTALF 261 P +E++ + L +S GIE G IR +A L W++ + C+ KGV+ +GKVVYFT+LF Sbjct: 182 DPVKEFWERRTLQISDGIENIGSIRWELAGTLAVVWIMCYFCIWKGVKWTGKVVYFTSLF 241 Query: 262 PYVVLVILFFRGVTLPGAITGILFYLTPDFSQLANAQVWGDAAVQIFFALSPAWGGLITL 321 PY +L IL RG+TLPGA+ G+ +Y+TP+ S+L++ +VW DA QIFF+ + G L+ L Sbjct: 242 PYALLTILLIRGLTLPGAMEGLKYYVTPNLSKLSDPEVWIDAVTQIFFSYALGLGALVAL 301 Query: 322 SSYNKFSNNCYIDSLIVAVSNIATSFFAGLVIFSVIGFLAHELNVSVDRVVDQGAGLAFI 381 SYNKF+NN Y D+LIV N TS +G+VIFSV+GF+AHE V V G GLAF+ Sbjct: 302 GSYNKFNNNVYKDALIVCTVNSCTSMLSGIVIFSVVGFMAHEQQKPVADVAVSGPGLAFL 361 Query: 382 VYPEVVTRLPISPLWSILFFVMLLTLGLDSQFALMETVTTAILDRFPN-FRQKKGWVVFI 440 VYP V LP S +WS LFF ML+ +GLDSQF +E TA +D +P R++K + I Sbjct: 362 VYPSAVLELPGSSIWSCLFFFMLILIGLDSQFCTVEGFITAAVDEWPRLLRKRKELFIAI 421 Query: 441 VAVFGYLGGLIFTTNSGMYWLQLMDKYA-ANWSVLIIAIGECILIAWIYGAEKFCGDIQR 499 V YL GL T GMY QL+D YA + + +L + ECI I+W +G +F I+ Sbjct: 422 VCFVSYLIGLFCITEGGMYVFQLLDSYAVSGFCLLFLMFFECIAISWAFGVNRFYDGIRD 481 Query: 500 MIGRQSKLWVYLWSAMWRIITPAALVFILVFNWIEYKPASYGHYVYPMWADAVGW 554 MIG W W W I TPA V + FN I++ P Y Y YP W+ +GW Sbjct: 482 MIGYYPCCW---WKICWTITTPAICVGVFTFNIIKFVPVKYLTYEYPWWSHVLGW 533 >AY395073-1|AAQ96729.1| 203|Apis mellifera GABA neurotransmitter transporter-1A protein. Length = 203 Score = 173 bits (422), Expect = 3e-45 Identities = 87/212 (41%), Positives = 130/212 (61%), Gaps = 16/212 (7%) Query: 48 NVWRFPYLCYNNGGGAFLIPFTVMLVIAGLPLMFMELSFGQYAALGPVAVYNKFCPLFRG 107 NVWRFPYLCY NGGGAFLIP+ + LV+AG+P+ FMEL+ GQ +G + V+ K PLF+G Sbjct: 3 NVWRFPYLCYKNGGGAFLIPYFLTLVLAGIPMFFMELALGQMLTVGGLGVF-KIAPLFKG 61 Query: 108 LGYGMVIVSSIVMLYYNLIIAWTIFYMTVSFSSIFYQLPWQNCKAEWSTKHCYSYEEAD- 166 +GY ++S + +YY +I+AW IFY +S S +LPW +C W+TK+C + + D Sbjct: 62 IGYAAAVMSCWMNVYYIVILAWAIFYFFMSMRS---ELPWGSCNNYWNTKNCVNPYDRDS 118 Query: 167 -ECEAQNGTY--YLRQCFNQSYSVSHNITALAKTALRRPPAEEYFTNQVLGLSSGIEETG 223 C Q + +++ C V+ IT L P +E++ + L +S G+E G Sbjct: 119 LSCWLQMTKHHNFIKVC--SVNDVNMTITELT------DPVKEFWERRALQISEGVEYVG 170 Query: 224 EIRLGMAMCLLAAWVIVFLCLCKGVQSSGKVV 255 IR +A LL W++ + C+ KGV+++GKVV Sbjct: 171 NIRWELAGTLLLVWILCYFCIWKGVKTTGKVV 202 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.327 0.141 0.455 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 176,470 Number of Sequences: 429 Number of extensions: 7079 Number of successful extensions: 42 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 22 Number of HSP's gapped (non-prelim): 3 length of query: 632 length of database: 140,377 effective HSP length: 62 effective length of query: 570 effective length of database: 113,779 effective search space: 64854030 effective search space used: 64854030 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.7 bits) S2: 47 (23.0 bits)
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