Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000903-TA|BGIBMGA000903-PA|IPR000717|Proteasome
component region PCI
(386 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g02200.2 68416.m00199 proteasome family protein contains Pfam... 132 5e-31
At5g15610.2 68418.m01827 proteasome family protein contains Pfam... 128 6e-30
At3g02200.1 68416.m00198 proteasome family protein contains Pfam... 107 1e-23
At5g15610.1 68418.m01826 proteasome family protein contains Pfam... 103 3e-22
At4g19006.1 68417.m02801 26S proteasome regulatory subunit, puta... 53 3e-07
At5g45620.1 68418.m05608 26S proteasome regulatory subunit, puta... 49 5e-06
At4g00190.1 68417.m00020 pectinesterase family protein contains ... 33 0.43
At5g66540.1 68418.m08389 expressed protein ; supported by full-L... 30 3.0
At1g67680.1 68414.m07723 expressed protein 29 4.0
At1g67650.1 68414.m07720 expressed protein 29 4.0
At1g76850.1 68414.m08943 expressed protein 29 5.3
At2g31890.1 68415.m03896 expressed protein 29 6.9
At5g39720.1 68418.m04809 avirulence-responsive protein-related /... 28 9.2
At4g36710.1 68417.m05209 scarecrow transcription factor family p... 28 9.2
At3g55160.1 68416.m06126 expressed protein 28 9.2
>At3g02200.2 68416.m00199 proteasome family protein contains Pfam
domain, PF01399: PCI domain
Length = 417
Score = 132 bits (318), Expect = 5e-31
Identities = 86/316 (27%), Positives = 165/316 (52%), Gaps = 14/316 (4%)
Query: 58 SESDIEAILNSIVSIMVSIPL-ERGENLILAFSQRLTKAPGPKLGMVALQSLWRLYNNLE 116
SE D+E I SI+ + E+ ++ A + ++ + P K + L+ L+ LYN L
Sbjct: 72 SEKDLECTYTIICSIVKNAKSPEQVLEMVKAIASKVAQQPSDKASL-RLKILFNLYN-LV 129
Query: 117 PNSPLRYHVYYHVIELAARVGFVREVFTGVEQLRKEFANCPPSNEQMQKLYRLLHQVLKD 176
+ R+ VY + LA + + +++ + ++L+ + VLK+
Sbjct: 130 DHPYARFQVYMKALTLAVDGKVTEYIVSSFKKIDNFLKEWNIDIKDQRELFLAIANVLKE 189
Query: 177 QNSEL--AAKVMIELLGTYTDENASY---AREDAIKCIATALADPNTFLLDPLLTLKPVR 231
S + + K + + L T+++E+A A+E+A++ + + + F D LL L V
Sbjct: 190 NKSLVNESLKFLTKYLATFSNEDAQVLDEAKEEAVRAVIEFVKASSIFQCD-LLDLPAVA 248
Query: 232 FLEGEL----IHDLLTIFVSEKLSSYQTFYNNHKEFVHSQGLNHEQNIKKMRILSFMQMA 287
LE + ++ LL IF++++L++Y F N + F+ S GL++E + KMR+LS + +A
Sbjct: 249 QLEKDAKYAPVYQLLKIFLTQRLNAYTEFQNANSGFLQSYGLSNEDCVTKMRLLSLVDLA 308
Query: 288 -ETNPDLTFDEIISELQIEEKDVEAFVIEVLKTRLVRARMDQAQRTVRVSNTMHRTFGRE 346
+ + + + I LQ+ E+DVE ++++ + +L+ +MDQ + + VS + R FG +
Sbjct: 309 SDESGKIPYTSIKDTLQVNEQDVELWIVKAITAKLIECKMDQMNQVLIVSRSSEREFGTK 368
Query: 347 QWQQLRDVLLAWRANV 362
QWQ LR L W+ N+
Sbjct: 369 QWQSLRTKLATWKDNI 384
>At5g15610.2 68418.m01827 proteasome family protein contains Pfam
domain, PF01399: PCI domain
Length = 413
Score = 128 bits (309), Expect = 6e-30
Identities = 88/326 (26%), Positives = 169/326 (51%), Gaps = 16/326 (4%)
Query: 58 SESDIEAILNSIVSIMVSIPL-ERGENLILAFSQRLTKAPGPKLGMVALQSLWRLYNNLE 116
SE D+E I +++ + E ++ A + ++ + P K + L+ L+ LYN ++
Sbjct: 72 SEKDLECTYTIICNLVKNANSPEEVLEMVKAIAAKVIQQPNDKASL-RLKILFNLYNLVD 130
Query: 117 -PNSPLRYHVYYHVIELAARVGFVREVFTGVEQLRKEFANCPPSNEQMQKLYRLLHQVLK 175
PN+ R+ VY +ELA + +++ + ++L+ + +VL+
Sbjct: 131 HPNA--RFQVYMKALELAVNGKVTEYIVPSFKKIDSFLKEWNIDIKDQRELFLAIAKVLR 188
Query: 176 DQNS--ELAAKVMIELLGTYTDENA---SYAREDAIKCIATALADPNTFLLDPLLTLKPV 230
+ S + + + + L T+++E+ S A+E+A++ + + P+ F D LL V
Sbjct: 189 ENKSFAKESLQFVTNYLATFSNEDTQVLSEAKEEAVRAVIEFVKAPSIFQCD-LLDHPAV 247
Query: 231 RFLEGEL----IHDLLTIFVSEKLSSYQTFYNNHKEFVHSQGLNHEQNIKKMRILSFMQM 286
LE + ++ LL IF++++L +Y F N + F+ + GL E + KMR+LS + +
Sbjct: 248 AQLEKDPNNAPVYQLLKIFLTQRLDAYMEFQNANSGFLQTYGLVEEDCVAKMRLLSLVDL 307
Query: 287 A-ETNPDLTFDEIISELQIEEKDVEAFVIEVLKTRLVRARMDQAQRTVRVSNTMHRTFGR 345
A + + + + I + LQ+ +++VE +V++ + +LV +MDQ + V VS R FG+
Sbjct: 308 ASDDSGKIPYASIKNTLQVNDEEVELWVVKAITAKLVACKMDQMNQVVIVSRCAEREFGQ 367
Query: 346 EQWQQLRDVLLAWRANVHQAHEAMKS 371
+QWQ LR L AWR NV ++S
Sbjct: 368 KQWQSLRTKLAAWRDNVRNVISTIES 393
>At3g02200.1 68416.m00198 proteasome family protein contains Pfam
domain, PF01399: PCI domain
Length = 364
Score = 107 bits (257), Expect = 1e-23
Identities = 76/296 (25%), Positives = 152/296 (51%), Gaps = 14/296 (4%)
Query: 58 SESDIEAILNSIVSIMVSIPL-ERGENLILAFSQRLTKAPGPKLGMVALQSLWRLYNNLE 116
SE D+E I SI+ + E+ ++ A + ++ + P K + L+ L+ LYN L
Sbjct: 72 SEKDLECTYTIICSIVKNAKSPEQVLEMVKAIASKVAQQPSDKASL-RLKILFNLYN-LV 129
Query: 117 PNSPLRYHVYYHVIELAARVGFVREVFTGVEQLRKEFANCPPSNEQMQKLYRLLHQVLKD 176
+ R+ VY + LA + + +++ + ++L+ + VLK+
Sbjct: 130 DHPYARFQVYMKALTLAVDGKVTEYIVSSFKKIDNFLKEWNIDIKDQRELFLAIANVLKE 189
Query: 177 QNSEL--AAKVMIELLGTYTDENASY---AREDAIKCIATALADPNTFLLDPLLTLKPVR 231
S + + K + + L T+++E+A A+E+A++ + + + F D LL L V
Sbjct: 190 NKSLVNESLKFLTKYLATFSNEDAQVLDEAKEEAVRAVIEFVKASSIFQCD-LLDLPAVA 248
Query: 232 FLEGEL----IHDLLTIFVSEKLSSYQTFYNNHKEFVHSQGLNHEQNIKKMRILSFMQMA 287
LE + ++ LL IF++++L++Y F N + F+ S GL++E + KMR+LS + +A
Sbjct: 249 QLEKDAKYAPVYQLLKIFLTQRLNAYTEFQNANSGFLQSYGLSNEDCVTKMRLLSLVDLA 308
Query: 288 -ETNPDLTFDEIISELQIEEKDVEAFVIEVLKTRLVRARMDQAQRTVRVSNTMHRT 342
+ + + + I LQ+ E+DVE ++++ + +L+ +MDQ + + V + RT
Sbjct: 309 SDESGKIPYTSIKDTLQVNEQDVELWIVKAITAKLIECKMDQMNQVLIVRQPLFRT 364
>At5g15610.1 68418.m01826 proteasome family protein contains Pfam
domain, PF01399: PCI domain
Length = 442
Score = 103 bits (246), Expect = 3e-22
Identities = 89/355 (25%), Positives = 172/355 (48%), Gaps = 45/355 (12%)
Query: 58 SESDIEAILNSIVSIMVSIPL-ERGENLILAFSQRLTKAPGPKLGMVALQSLWRLYNNLE 116
SE D+E I +++ + E ++ A + ++ + P K + L+ L+ LYN ++
Sbjct: 72 SEKDLECTYTIICNLVKNANSPEEVLEMVKAIAAKVIQQPNDKASL-RLKILFNLYNLVD 130
Query: 117 -PNSPLRYHVYYHVIELAARVGFVREVFTGVEQLRKEFANCPPSNEQMQKLYRLLHQVLK 175
PN+ R+ VY +ELA + +++ + ++L+ + +VL+
Sbjct: 131 HPNA--RFQVYMKALELAVNGKVTEYIVPSFKKIDSFLKEWNIDIKDQRELFLAIAKVLR 188
Query: 176 DQNS--ELAAKVMIELLGTYTDENA---SYAREDAIKCIATALADPNTFLLDPLLTLKPV 230
+ S + + + + L T+++E+ S A+E+A++ + + P+ F D LL V
Sbjct: 189 ENKSFAKESLQFVTNYLATFSNEDTQVLSEAKEEAVRAVIEFVKAPSIFQCD-LLDHPAV 247
Query: 231 RFLEGEL----IHDLLTIFVSEKLSSYQTFYNNHKEFVHSQGLNHEQNIKKMRILSFMQM 286
LE + ++ LL IF++++L +Y F N + F+ + GL E + KMR+LS + +
Sbjct: 248 AQLEKDPNNAPVYQLLKIFLTQRLDAYMEFQNANSGFLQTYGLVEEDCVAKMRLLSLVDL 307
Query: 287 A-ETNPDLTFDEIISELQIEEKDVEAFVIEVLKTRLVRARMDQAQRTV---RVSNTM--- 339
A + + + + I + LQ+ +++VE +V++ + +LV +MDQ + V +VSN +
Sbjct: 308 ASDDSGKIPYASIKNTLQVNDEEVELWVVKAITAKLVACKMDQMNQVVIVRQVSNLLLFR 367
Query: 340 -----------------------HRTFGREQWQQLRDVLLAWRANVHQAHEAMKS 371
R FG++QWQ LR L AWR NV ++S
Sbjct: 368 FICVNPKSHILVLHICSCISRCAEREFGQKQWQSLRTKLAAWRDNVRNVISTIES 422
>At4g19006.1 68417.m02801 26S proteasome regulatory subunit,
putative (RPN9) similar to 26S proteasome subunit p40.5
[Homo sapiens] gi|3618343|dbj|BAA33214
Length = 386
Score = 53.2 bits (122), Expect = 3e-07
Identities = 54/204 (26%), Positives = 92/204 (45%), Gaps = 12/204 (5%)
Query: 171 HQVLKDQNSELAAKVMIELLGTYTDE-NASYAREDAIKCIATALADPNTFLLDPLLTLKP 229
H+V + + SE ++ L T D + S+ + A +AL N + LL
Sbjct: 168 HKV-RQEFSEFYKNALLYLAYTSVDSLSESFKLDLAFDLSLSALLGENIYNFGELLAHPI 226
Query: 230 VRFLEG---ELIHDLLTIFVSEKLSSYQTFYNNHKEFVHSQGL---NHEQNIKKMRILSF 283
++ L G E ++ +L F L YQ H + +Q N ++ ++K+ IL
Sbjct: 227 LKSLLGTNVEWLYHILQAFNHGDLVQYQELCRVHNASLSAQPALVENEKKLLEKINILCL 286
Query: 284 MQMAETNP--DLTFD-EIISE-LQIEEKDVEAFVIEVLKTRLVRARMDQAQRTVRVSNTM 339
+++ + P D T +I+E ++ +DVE +++ L L+ +DQ TV VS
Sbjct: 287 IEIIFSRPAEDRTIPLSVIAERTKLSIEDVEHLLMKSLSVHLIEGILDQVNGTVYVSWAQ 346
Query: 340 HRTFGREQWQQLRDVLLAWRANVH 363
R G Q + LRD L +W VH
Sbjct: 347 PRVLGIPQIKSLRDQLDSWVDKVH 370
>At5g45620.1 68418.m05608 26S proteasome regulatory subunit,
putative (RPN9) contains similarity to 26S proteasome
subunit p40.5 GI:3618343 from [Homo sapiens]
Length = 386
Score = 49.2 bits (112), Expect = 5e-06
Identities = 48/179 (26%), Positives = 81/179 (45%), Gaps = 10/179 (5%)
Query: 195 DENASYAREDAIKCIATALADPNTFLLDPLLTLKPVRFLEG---ELIHDLLTIFVSEKLS 251
D + S+ + A +AL N + LL ++ L G E ++ +L F L
Sbjct: 192 DLSESFKLDLAFDLSLSALLGENIYNFGELLAHPILKSLLGTNVEWLYHILQAFNHGDLV 251
Query: 252 SYQTFYNNHKEFVHSQGL---NHEQNIKKMRILSFMQMAETNP--DLTFD-EIISE-LQI 304
YQ H + +Q N ++ ++K+ IL +++ + P D T II+E ++
Sbjct: 252 QYQELCRVHNASLIAQPALVENEKKLLEKINILCLIEIIFSRPAEDRTIPLSIIAERTKL 311
Query: 305 EEKDVEAFVIEVLKTRLVRARMDQAQRTVRVSNTMHRTFGREQWQQLRDVLLAWRANVH 363
+DVE +++ L L+ +DQ T+ VS R G Q + LRD L +W VH
Sbjct: 312 SIEDVEHLLMKSLSVHLIEGIIDQVNGTIYVSWAQPRVLGIPQIKALRDQLDSWVDKVH 370
>At4g00190.1 68417.m00020 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 474
Score = 32.7 bits (71), Expect = 0.43
Identities = 18/63 (28%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
Query: 280 ILSFMQMAETNPDLTFDEIISELQIEEKDVEAFVIEVLKTRLVRARMDQAQRTVRVSNTM 339
+ +++ A TN D T E +SEL + +E+ I+V+ T + RM+Q + +S
Sbjct: 95 VQAWLSTALTNLD-TCQEEMSELGVSSHSLESITIDVINTLAINKRMEQNGKEFGISKIT 153
Query: 340 HRT 342
+T
Sbjct: 154 MKT 156
>At5g66540.1 68418.m08389 expressed protein ; supported by
full-Length cDNA gi:12057175 from [Arabidopsis thaliana]
Length = 524
Score = 29.9 bits (64), Expect = 3.0
Identities = 13/44 (29%), Positives = 25/44 (56%)
Query: 292 DLTFDEIISELQIEEKDVEAFVIEVLKTRLVRARMDQAQRTVRV 335
DL F+ + ++V A + +++K+R++ AR D QR R+
Sbjct: 326 DLDFEHNARPAPVITEEVTASLEDLIKSRIIEARFDDVQRAPRL 369
>At1g67680.1 68414.m07723 expressed protein
Length = 664
Score = 29.5 bits (63), Expect = 4.0
Identities = 19/57 (33%), Positives = 28/57 (49%), Gaps = 6/57 (10%)
Query: 163 MQKLYRLLHQVLKDQNSELAAKVMIELLGTYTDENASYAREDAIKCIATALADPNTF 219
++ L+ LHQ +KD E A KV ++L + EDAI+C AL + F
Sbjct: 20 IEDLFTSLHQHIKDTKYEEAVKVADQVLSIVPTD------EDAIRCKVVALIKDDKF 70
>At1g67650.1 68414.m07720 expressed protein
Length = 651
Score = 29.5 bits (63), Expect = 4.0
Identities = 21/69 (30%), Positives = 31/69 (44%), Gaps = 6/69 (8%)
Query: 151 KEFANCPPSNEQMQKLYRLLHQVLKDQNSELAAKVMIELLGTYTDENASYAREDAIKCIA 210
K N S ++ L+ LH+ +KD E A KV ++L + EDAI+C
Sbjct: 4 KSKENPKTSTPAIEDLFTSLHKHIKDTKYEEAVKVADQVLSIVPTD------EDAIRCKV 57
Query: 211 TALADPNTF 219
AL + F
Sbjct: 58 VALIKDDKF 66
>At1g76850.1 68414.m08943 expressed protein
Length = 1090
Score = 29.1 bits (62), Expect = 5.3
Identities = 21/60 (35%), Positives = 32/60 (53%), Gaps = 2/60 (3%)
Query: 66 LNSIVSIMVSIPLERGENLILAFSQRLTKA-PGPKLGMVALQSLWRLYNNLEPNSPLRYH 124
L S V+I+ + LE E ++L F L K+ PK+ +L++ RL LEP S +H
Sbjct: 380 LPSHVNILKRV-LEEVEKVMLEFKGTLYKSMEDPKIDFTSLENTVRLLLELEPESDPVWH 438
>At2g31890.1 68415.m03896 expressed protein
Length = 671
Score = 28.7 bits (61), Expect = 6.9
Identities = 33/118 (27%), Positives = 55/118 (46%), Gaps = 9/118 (7%)
Query: 7 FMDISLEDQALELRRYFKSLGAEISEEKSPKGIEDDLHKIVGVCDACFKEPSESDIEAIL 66
F D +ED+ +LR+ SL + EEK K E ++ + F PS+ E L
Sbjct: 198 FEDGFVEDKMGDLRKRVSSLAGGMFEEKKEKMKEQLAQRL-----SQFSGPSDRMKEINL 252
Query: 67 N-SIVSIMVSIP-LERGENLILAFSQRLTKAPGPKLGMVALQSLWRLYNNLEPNSPLR 122
N +I+ + LE I+A ++ L+ +P L + +L R+ N+E S +R
Sbjct: 253 NKAIIEAQTAEEVLEVTAETIMAVAKGLSPSPLSPLNIAT--ALHRIAKNMEKVSMMR 308
>At5g39720.1 68418.m04809 avirulence-responsive protein-related /
avirulence induced gene (AIG) protein-related similar to
SP|P54121 AIG2 protein {Arabidopsis thaliana}
Length = 165
Score = 28.3 bits (60), Expect = 9.2
Identities = 16/60 (26%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
Query: 234 EGELIHDLLTIFVSEKLSSYQTFYNNHKEFVHSQGLNHEQNIKKMRILSFMQMAETNPDL 293
+GE+ +L S++L + N E V + G+ E N +KM + ++M + + +PD+
Sbjct: 60 KGEVHGKVLMGVTSDELENLDAVEGNEYERV-TVGIVREDNSEKMAVKTYMWINKADPDM 118
>At4g36710.1 68417.m05209 scarecrow transcription factor family
protein
Length = 486
Score = 28.3 bits (60), Expect = 9.2
Identities = 13/36 (36%), Positives = 21/36 (58%)
Query: 224 LLTLKPVRFLEGELIHDLLTIFVSEKLSSYQTFYNN 259
+L+ K +RF+EGE L++ + +LS F NN
Sbjct: 306 MLSFKAIRFVEGERTVVLISPAIFRRLSGITDFVNN 341
>At3g55160.1 68416.m06126 expressed protein
Length = 2149
Score = 28.3 bits (60), Expect = 9.2
Identities = 42/182 (23%), Positives = 78/182 (42%), Gaps = 24/182 (13%)
Query: 46 IVGVCDACFKEPSESDIEAILNSIVSIMVSIPLERGENLILAFSQRLTKAPGPKLGMVAL 105
++ +CD C E+ I++ LN V ++ I +++ + +L L++ P +A
Sbjct: 355 LLELCDLC-----ENPIDSHLNFHVLTVMQICMQQIKTSMLT---DLSEGYDPMPDSMAA 406
Query: 106 QSLWRLYNNLE-PNSPL--RYHVYYHVI--------ELAARVGFVREVFTGVEQLRKEFA 154
+ L ++NNLE P S + H+ + ++ + +VG + V L + +
Sbjct: 407 RVLRIIWNNLEDPLSQTVKQVHLMFDLLLDIQTTVHQTDDKVGMRESLLKIVNYLLRLGS 466
Query: 155 NCPPSNEQMQKLYRLLH-QVLKDQNSELAAKVMIELLGTYTDENASYAREDAIKCIATAL 213
C + L R L + L D + L + E+ Y D++ YA IKC L
Sbjct: 467 RCKGRYVPLASLTRRLGAKTLMDMSPNL----LFEMANAYIDDDVCYAVTSFIKCFLELL 522
Query: 214 AD 215
D
Sbjct: 523 RD 524
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.320 0.134 0.381
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,329,495
Number of Sequences: 28952
Number of extensions: 335236
Number of successful extensions: 1016
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1002
Number of HSP's gapped (non-prelim): 17
length of query: 386
length of database: 12,070,560
effective HSP length: 82
effective length of query: 304
effective length of database: 9,696,496
effective search space: 2947734784
effective search space used: 2947734784
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 60 (28.3 bits)
- SilkBase 1999-2023 -