BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000891-TA|BGIBMGA000891-PA|IPR006201|Neurotransmitter- gated ion-channel, IPR006028|Gamma-aminobutyric acid A receptor, IPR001390|Gamma-aminobutyric-acid A receptor, alpha subunit, IPR006202|Neurotransmitter-gated ion-channel ligand-binding, IPR006029|Neurotransmitter-gated ion-channel transmembrane region (539 letters) Database: tribolium 317 sequences; 114,650 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value AF025669-1|AAB81523.1| 243|Tribolium castaneum GABA receptor su... 142 6e-36 EF127808-1|ABL67945.1| 311|Tribolium castaneum nicotinic acetyl... 76 7e-16 EF127806-1|ABL67943.1| 311|Tribolium castaneum nicotinic acetyl... 75 2e-15 EF127807-1|ABL67944.1| 311|Tribolium castaneum nicotinic acetyl... 74 2e-15 EF127810-1|ABL67947.1| 311|Tribolium castaneum nicotinic acetyl... 72 9e-15 EF127809-1|ABL67946.1| 311|Tribolium castaneum nicotinic acetyl... 72 9e-15 S78567-1|AAB34900.1| 141|Tribolium castaneum GABA receptor subu... 69 8e-14 AY695256-1|AAW21973.1| 168|Tribolium castaneum ventral nerve co... 26 0.76 DQ659251-1|ABG47449.1| 366|Tribolium castaneum chitinase 11 pro... 23 7.1 >AF025669-1|AAB81523.1| 243|Tribolium castaneum GABA receptor subunit protein. Length = 243 Score = 142 bits (344), Expect = 6e-36 Identities = 70/201 (34%), Positives = 116/201 (57%), Gaps = 4/201 (1%) Query: 145 NITSVLENLLKNYENSQLPTHGKGYPTVVQTNILIRSMGPVSELDMDYSMDCYFRQYWRD 204 NI+++L++ +Y+ P +G G P V + + S+ +SE+ MD+++D YFRQ+W D Sbjct: 40 NISAILDSFSVSYDKRVRPNYG-GPPVEVGVTMYVLSISSLSEVKMDFTLDFYFRQFWTD 98 Query: 205 TRLSF--LGPIRSLSLSIKMLERIWRPDTYFYNGKHSYVHTITVPNKLLRISQHGDILYS 262 RL++ + +LS+ + ++ IW PDT+F N K SY H T N+ +R+ G I S Sbjct: 99 PRLAYGKRPGVETLSVGSEFIKNIWVPDTFFVNEKQSYFHIATTSNEFIRVHHSGSITRS 158 Query: 263 MRLTIKAKCPMELRNFPMDRQSCPLILGSYAYSNQQLVYQW-QNSQSVNFVPGMTLSQFD 321 +RLTI A CPM L+ PMDRQ C + + S+ Y+ + + Y+W + SV ++L QF Sbjct: 159 IRLTITASCPMNLQYLPMDRQLCHIEIESFGYTMRDIRYKWNEGPNSVGVSSEVSLPQFK 218 Query: 322 LISFPYRNFTFTRREGDFSVL 342 ++ R + G++S L Sbjct: 219 VLGHRRRAVEISLTTGNYSRL 239 >EF127808-1|ABL67945.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 3 protein. Length = 311 Score = 75.8 bits (178), Expect = 7e-16 Identities = 55/239 (23%), Positives = 101/239 (42%), Gaps = 11/239 (4%) Query: 149 VLENLLKNYENSQLPTHGKGYPTVVQTNILIRSMGPVSELDMDYSMDCYFRQYWRDTRLS 208 +L NLL Y + P + P V+ + ++ + V E + + + + W D L Sbjct: 7 LLNNLLGPYNVLERPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLK 66 Query: 209 F----LGPIRSLSLSIKMLERIWRPDTYFYNGKHSYVHTITVPNKLLRISQHGDILYSMR 264 + G ++ L ++ ++W+PD YN N +++ +G LY Sbjct: 67 WNESEYGGVKDLRITPN---KLWKPDVLMYNSADEGFDGTFQTNVVVK--HNGSCLYVPP 121 Query: 265 LTIKAKCPMELRNFPMDRQSCPLILGSYAYSNQQLVYQWQNSQSVNFVPGMTLSQFDLIS 324 K+ C M++ FP D Q C + GS+ Y QL + + +T ++ LI Sbjct: 122 GIFKSTCKMDITWFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIG 181 Query: 325 FPYRNFTFTRREGDFSVLQVSFNLK--RHTGYFLIQVYVPCILIVVLSWVSFWIHREVG 381 P + T + V+F +K R T Y+ + VPC+LI ++ + F + + G Sbjct: 182 MPGKKNTIVYACCPEPYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSG 240 >EF127806-1|ABL67943.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 1 protein. Length = 311 Score = 74.5 bits (175), Expect = 2e-15 Identities = 55/239 (23%), Positives = 100/239 (41%), Gaps = 11/239 (4%) Query: 149 VLENLLKNYENSQLPTHGKGYPTVVQTNILIRSMGPVSELDMDYSMDCYFRQYWRDTRLS 208 +L NLL Y + P + P V+ + ++ + V E + + + + W D L Sbjct: 7 LLNNLLGPYNVLERPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLK 66 Query: 209 F----LGPIRSLSLSIKMLERIWRPDTYFYNGKHSYVHTITVPNKLLRISQHGDILYSMR 264 + G ++ L ++ ++W+PD YN N +++ G LY Sbjct: 67 WNESEYGGVKDLRITPN---KLWKPDVLMYNSADEGFDGTFQTNVVVK--HGGSCLYVPP 121 Query: 265 LTIKAKCPMELRNFPMDRQSCPLILGSYAYSNQQLVYQWQNSQSVNFVPGMTLSQFDLIS 324 K+ C M++ FP D Q C + GS+ Y QL + + +T ++ LI Sbjct: 122 GIFKSTCKMDITWFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIG 181 Query: 325 FPYRNFTFTRREGDFSVLQVSFNLK--RHTGYFLIQVYVPCILIVVLSWVSFWIHREVG 381 P + T + V+F +K R T Y+ + VPC+LI ++ + F + + G Sbjct: 182 MPGKKNTIVYACCPEPYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSG 240 >EF127807-1|ABL67944.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 2 protein. Length = 311 Score = 74.1 bits (174), Expect = 2e-15 Identities = 54/239 (22%), Positives = 101/239 (42%), Gaps = 11/239 (4%) Query: 149 VLENLLKNYENSQLPTHGKGYPTVVQTNILIRSMGPVSELDMDYSMDCYFRQYWRDTRLS 208 +L NLL Y + P + P V+ + ++ + V E + + + + W D L Sbjct: 7 LLNNLLGPYNVLERPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLK 66 Query: 209 F----LGPIRSLSLSIKMLERIWRPDTYFYNGKHSYVHTITVPNKLLRISQHGDILYSMR 264 + G ++ L ++ ++W+PD YN N +++ +G LY Sbjct: 67 WNESEYGGVKDLRITPN---KLWKPDVLMYNSADEGFDGTFQTNVVVK--HNGSCLYVPP 121 Query: 265 LTIKAKCPMELRNFPMDRQSCPLILGSYAYSNQQLVYQWQNSQSVNFVPGMTLSQFDLIS 324 K+ C +++ FP D Q C + GS+ Y QL + + +T ++ LI Sbjct: 122 GIFKSTCKIDITWFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIG 181 Query: 325 FPYRNFTFTRREGDFSVLQVSFNLK--RHTGYFLIQVYVPCILIVVLSWVSFWIHREVG 381 P + T + V+F +K R T Y+ + VPC+LI ++ + F + + G Sbjct: 182 MPGKKNTIVYACCPEPYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSG 240 >EF127810-1|ABL67947.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 5 protein. Length = 311 Score = 72.1 bits (169), Expect = 9e-15 Identities = 55/239 (23%), Positives = 99/239 (41%), Gaps = 11/239 (4%) Query: 149 VLENLLKNYENSQLPTHGKGYPTVVQTNILIRSMGPVSELDMDYSMDCYFRQYWRDTRLS 208 +L NLL Y + P + P V+ + ++ + V E + + + W D L Sbjct: 7 LLNNLLGPYNVLERPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNIWLSLEWNDYNLK 66 Query: 209 F----LGPIRSLSLSIKMLERIWRPDTYFYNGKHSYVHTITVPNKLLRISQHGDILYSMR 264 + G ++ L ++ ++W+PD YN N +++ G LY Sbjct: 67 WNESEYGGVKDLRITPN---KLWKPDVLMYNSADEGFDGTFQTNVVVK--HGGSCLYVPP 121 Query: 265 LTIKAKCPMELRNFPMDRQSCPLILGSYAYSNQQLVYQWQNSQSVNFVPGMTLSQFDLIS 324 K+ C M++ FP D Q C + GS+ Y QL + + +T ++ LI Sbjct: 122 GIFKSTCKMDITWFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIG 181 Query: 325 FPYRNFTFTRREGDFSVLQVSFNLK--RHTGYFLIQVYVPCILIVVLSWVSFWIHREVG 381 P + T + V+F +K R T Y+ + VPC+LI ++ + F + + G Sbjct: 182 MPGKKNTIVYACCPEPYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSG 240 >EF127809-1|ABL67946.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 4 protein. Length = 311 Score = 72.1 bits (169), Expect = 9e-15 Identities = 55/239 (23%), Positives = 99/239 (41%), Gaps = 11/239 (4%) Query: 149 VLENLLKNYENSQLPTHGKGYPTVVQTNILIRSMGPVSELDMDYSMDCYFRQYWRDTRLS 208 +L NLL Y + P + P V+ + ++ + V E + + + W D L Sbjct: 7 LLNNLLGPYNVLERPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNIWLSLEWNDYNLK 66 Query: 209 F----LGPIRSLSLSIKMLERIWRPDTYFYNGKHSYVHTITVPNKLLRISQHGDILYSMR 264 + G ++ L ++ ++W+PD YN N +++ G LY Sbjct: 67 WNESEYGGVKDLRITPN---KLWKPDVLMYNSADEGFDGTFQTNVVVK--HGGSCLYVPP 121 Query: 265 LTIKAKCPMELRNFPMDRQSCPLILGSYAYSNQQLVYQWQNSQSVNFVPGMTLSQFDLIS 324 K+ C M++ FP D Q C + GS+ Y QL + + +T ++ LI Sbjct: 122 GIFKSTCKMDITWFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIG 181 Query: 325 FPYRNFTFTRREGDFSVLQVSFNLK--RHTGYFLIQVYVPCILIVVLSWVSFWIHREVG 381 P + T + V+F +K R T Y+ + VPC+LI ++ + F + + G Sbjct: 182 MPGKKNTIVYACCPEPYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDSG 240 >S78567-1|AAB34900.1| 141|Tribolium castaneum GABA receptor subunit protein. Length = 141 Score = 68.9 bits (161), Expect = 8e-14 Identities = 31/98 (31%), Positives = 53/98 (54%), Gaps = 1/98 (1%) Query: 291 SYAYSNQQLVYQW-QNSQSVNFVPGMTLSQFDLISFPYRNFTFTRREGDFSVLQVSFNLK 349 S+ Y+ + + Y+W + SV ++L QF ++ R + G++S L Sbjct: 5 SFGYTMRDIRYKWNEGPNSVGVSNEVSLPQFKVLGHRQRAMEISLTTGNYSRLACEIQFV 64 Query: 350 RHTGYFLIQVYVPCILIVVLSWVSFWIHREVGSGEIVI 387 R GY+LIQ+Y+P LIV++SWVSFW++R + + Sbjct: 65 RSMGYYLIQIYIPSGLIVIISWVSFWLNRNATPARVAL 102 >AY695256-1|AAW21973.1| 168|Tribolium castaneum ventral nerve cord defective protein protein. Length = 168 Score = 25.8 bits (54), Expect = 0.76 Identities = 21/75 (28%), Positives = 34/75 (45%), Gaps = 5/75 (6%) Query: 425 SMPSQSSNPTTSDNLETEQPVAVRLTMERTTQTET-RVPRWRQLLYCLAG----DDRYRR 479 ++PS +SNP S+ E T Q + R R++L+ A + R+R+ Sbjct: 2 NLPSSASNPAVSERDPDEDNSEDSKDPHLTDQQASGHKKRKRRVLFSKAQTYELERRFRQ 61 Query: 480 QRQREAGSRGHINSV 494 QR A R H+ S+ Sbjct: 62 QRYLSAPEREHLASI 76 >DQ659251-1|ABG47449.1| 366|Tribolium castaneum chitinase 11 protein. Length = 366 Score = 22.6 bits (46), Expect = 7.1 Identities = 12/42 (28%), Positives = 18/42 (42%) Query: 336 EGDFSVLQVSFNLKRHTGYFLIQVYVPCILIVVLSWVSFWIH 377 EG F ++ NL H Y + V L ++ SW +H Sbjct: 40 EGSFKPQNINANLCTHVHYAFLGVNSDGTLKILDSWNEIDLH 81 Database: tribolium Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 114,650 Number of sequences in database: 317 Lambda K H 0.319 0.133 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 124,506 Number of Sequences: 317 Number of extensions: 5176 Number of successful extensions: 31 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 12 Number of HSP's gapped (non-prelim): 10 length of query: 539 length of database: 114,650 effective HSP length: 60 effective length of query: 479 effective length of database: 95,630 effective search space: 45806770 effective search space used: 45806770 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 45 (22.2 bits)
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