Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000875-TA|BGIBMGA000875-PA|IPR002018|Carboxylesterase,
type B
(531 letters)
Database: mosquito
2123 sequences; 516,269 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CR954257-8|CAJ14159.1| 562|Anopheles gambiae putative esterase ... 182 2e-47
CR954257-9|CAJ14160.1| 573|Anopheles gambiae putative esterase ... 167 6e-43
AJ515149-1|CAD56156.1| 737|Anopheles gambiae acetylcholinestera... 133 1e-32
AJ515150-1|CAD56157.2| 737|Anopheles gambiae acetylcholinestera... 132 2e-32
AJ488492-1|CAD32684.2| 623|Anopheles gambiae acetylcholinestera... 132 2e-32
AJ535204-1|CAD59404.1| 1187|Anopheles gambiae SMC2 protein protein. 29 0.41
AJ439398-8|CAD28131.1| 1283|Anopheles gambiae putative different... 26 2.9
AY146716-1|AAO12076.1| 159|Anopheles gambiae odorant-binding pr... 25 5.0
L04753-1|AAA29357.1| 511|Anopheles gambiae alpha-amylase protein. 25 6.7
AJ535203-1|CAD59403.1| 1229|Anopheles gambiae SMC1 protein protein. 25 6.7
>CR954257-8|CAJ14159.1| 562|Anopheles gambiae putative esterase
protein.
Length = 562
Score = 182 bits (444), Expect = 2e-47
Identities = 154/527 (29%), Positives = 238/527 (45%), Gaps = 51/527 (9%)
Query: 26 VLISNQGLVRGHKAADG---DYSIFLGIPYAKVDVNNPFSTATDPIAFPEIIHDAVDE-V 81
++ ++ G ++G A+ G Y F GIPYA+ V P + + D +
Sbjct: 24 IINTSGGQIQGITASCGLFCSYFAFNGIPYAQPPVGELRFRNPRPHGGWQGVKDGSEHRS 83
Query: 82 RCPQTSSSVGEQ-TLNCLRLNIYVPSAASSRNPMPVLVWIHGGDFATGSASD--YGVKNI 138
CP G + +CL LN+Y + SR PV+VWIHGG F GS + YG N+
Sbjct: 84 TCPSGGFLGGVSGSEDCLYLNVYTQNLIGSR---PVMVWIHGGSFTGGSGNSWIYGPDNL 140
Query: 139 VRHGVLVVTMNYRLGPYGFLCLDVPTASGNQGLRDQYKALSWIRNNIASFGGNPYNVTIA 198
+ V+VVT+NYRLG GF D A+GN G++D AL W+R NIA+FGG+P NVTI
Sbjct: 141 MPEDVVVVTINYRLGILGFFSTDDVHAAGNWGMKDCVMALQWVRQNIAAFGGDPNNVTIF 200
Query: 199 GQDAGATSV-LLQLYSDNEKLFHKVIIESGTPQSEGMFVNADVEAAIKLAVHLGLNTTET 257
G+ AG +V L L + LFHK I +SGT F E A +LA G + ++
Sbjct: 201 GESAGGVAVHYLVLSNKASGLFHKAIAQSGTALVPWGFQYRPRELAYRLADRFGY-SHDS 259
Query: 258 EEAIQFLTKTSPDLVVAAAK---ELEL-------QLKPCRERSFSGISNFVESDPYSLTN 307
+Q L T + ++ + ++E+ P E S F+ P + N
Sbjct: 260 ATLVQSLRNTPIETLIETQEGWLDIEIPRGFKPFDFVPNAEPVNSPEETFLTQLPIDIIN 319
Query: 308 KKKVRNTPIMIGNTNKEKKSLSADY---------FSTDPFYEKLKSNFNLDEAQIEKAA- 357
+ P + G + E + ++ F+ +P Y + +N+ AA
Sbjct: 320 AGTFNHVPFIAGYMSMESLFMVYEHTIDSTVWNAFTRNPDY-FVPHFWNIPHGTAASAAV 378
Query: 358 -KNVRHFYIGDSSLSAEVASELQDFESDFVYNHPSQRTITQLLDENAGKIYEYLFSYSGE 416
+ +R+ Y D L ++ E F +D + + +T+ ++ Y Y FS+ G+
Sbjct: 379 SQGIRNAYWQDRPLGNDIMVEWLTFHTDQQFIYAIDKTVRLHAQRSSAPTYYYQFSFDGD 438
Query: 417 LE-----------DGAEHSSELNYLFE----TAGGSDRTEEEQLMVDRITTLWTNFVKFG 461
L GA H+ ++ YL+ T T + + +R L+TNF +FG
Sbjct: 439 LNLVKRVLMLGSWPGAMHADDIPYLWSVTDLTISPILPTNHARTVSNRFVRLFTNFARFG 498
Query: 462 NPTPKSTD--LIPTIWEPVTADTRPVLVLDKDVRLDSRIERQRMAYW 506
NPTP + D L W+PVTA T + + D+ QR A W
Sbjct: 499 NPTPNAVDTLLQSRQWQPVTAATVHYMDIGHDLVTGVNPNGQRTAVW 545
>CR954257-9|CAJ14160.1| 573|Anopheles gambiae putative esterase
protein.
Length = 573
Score = 167 bits (406), Expect = 6e-43
Identities = 155/538 (28%), Positives = 226/538 (42%), Gaps = 49/538 (9%)
Query: 26 VLISNQGLVRGHKAADG---DYSIFLGIPYAKVDVNNPFSTATDPIAFPEIIHDAVDE-V 81
++ S G V+G + G Y F GIPYA+ V + P A + D +
Sbjct: 38 IIDSPTGQVQGTTESCGLFCTYYSFKGIPYAEPPVGSLRFRNPVPRARWTGVRDGSNHGS 97
Query: 82 RCPQTSSSVGEQT--LNCLRLNIYVPSAASSRNPMPVLVWIHGGDFA--TGSASDYGVKN 137
C Q S G+ +CL LNIY R PV+VWIHGG ++ +G++ D+G +
Sbjct: 98 ECLQVSVVPGQVRGGEDCLYLNIYTQQLVGLR---PVMVWIHGGGYSINSGNSVDFGPEK 154
Query: 138 IVRHGVLVVTMNYRLGPYGFLCLDVPTASGNQGLRDQYKALSWIRNNIASFGGNPYNVTI 197
+V+ VL+VT+NYRLG GFL A+GN GL+D +AL W+R+NIA+FGG+P +VTI
Sbjct: 155 LVQDNVLLVTLNYRLGALGFLSTGDRYAAGNWGLKDCLQALRWVRSNIAAFGGDPNSVTI 214
Query: 198 AGQDAGATSVLLQLYSD-NEKLFHKVIIESGTPQSEGMFVNADVEAAIKLAVHLGLNTTE 256
G AGA V L + +D LFH+ I +S T F A ++A LG T
Sbjct: 215 FGNSAGAALVHLLVLTDAGAGLFHRAIAQSSTALVPYAFQTRPRFYADRIASALGFGTDS 274
Query: 257 --------TEEAIQFLTKTSPDLVVAAAKELE-LQLKPCRERSFSGISNFVESDPYSLTN 307
T A QF+ + + + L L P E + V P L
Sbjct: 275 STYVERLRTVPAEQFVPFQEATVTIPVPRFLRPLDFGPVVEPGDAPDEVIVRQRPIELI- 333
Query: 308 KKKVRNTPIMIGNTNKEKKSLSA----------DYFSTDP-FYEKLKSNFNLDEAQIEKA 356
+ + P ++G T+ E +A F+ +P + N A +
Sbjct: 334 RTRAHRVPFLVGYTDLEGAFFTALDNAIDPTVKGQFNANPHLFVPFFWNVGAGTAASSQI 393
Query: 357 AKNVRHFYIGDSSLSAEVASELQDFESDFVYNHPSQRTITQLLDENAGKIYEYLFSYSGE 416
+ R Y L A + E ++SD ++ +T+ +Y Y F+Y G+
Sbjct: 394 SGAFREHYWQSRPLDASLDYEWTVYQSDHMFAFAIDQTVRLHAQTTPAPLYYYQFAYDGD 453
Query: 417 LE----------DGAEHSSELNYLFETAGGS----DRTEEEQLMVDRITTLWTNFVKFGN 462
L GA H+ EL YLF + R+ +WTNF K GN
Sbjct: 454 LNLYKKLFGVQHPGAIHTDELPYLFHIPAAMLVPVSPDSHANTVSSRVVRMWTNFAKTGN 513
Query: 463 PTPKSTDLIPTI-WEPVTADTRPVLVLDKD-VRLDSRIERQRMAYWDLFFTTYGQYNF 518
PTP L+ + W V A L + D + + RM W TY F
Sbjct: 514 PTPGQDALLQNVQWPTVGATGTGYLSIGHDLLPVQQTPNPTRMNLWYNLQQTYANAPF 571
>AJ515149-1|CAD56156.1| 737|Anopheles gambiae acetylcholinesterase
protein.
Length = 737
Score = 133 bits (321), Expect = 1e-32
Identities = 142/531 (26%), Positives = 230/531 (43%), Gaps = 55/531 (10%)
Query: 23 DPLVLISNQGLVRGHK--AADGD-YSIFLGIPYAKVDVN-----NP---------FSTAT 65
DPLV+ +++G +RG A G ++LGIPYA+ V +P +T T
Sbjct: 164 DPLVVNTDKGRIRGITVDAPSGKKVDVWLGIPYAQPPVGPLRFRHPRPAEKWTGVLNTTT 223
Query: 66 DPIAFPEIIHDAVDEVRCPQTSSSVGEQTLNCLRLNIYVPSAASSRNPMPVLVWIHGGDF 125
P + +I+ + + + +CL +N+ P V++WI GG F
Sbjct: 224 PPNSCVQIVDTVFGDFPGATMWNPNTPLSEDCLYINVVAPRPRPKN--AAVMLWIFGGSF 281
Query: 126 ATGSAS--DYGVKNIV-RHGVLVVTMNYRLGPYGFLCLDVPTASGNQGLRDQYKALSWIR 182
+G+A+ Y + + V+VV++ YR+ GFL L P A GN GL DQ AL W+R
Sbjct: 282 YSGTATLDVYDHRALASEENVIVVSLQYRVASLGFLFLGTPEAPGNAGLFDQNLALRWVR 341
Query: 183 NNIASFGGNPYNVTIAGQDAGATSVLLQLYSD-NEKLFHKVIIESGTPQSEGMFVNADVE 241
+NI FGG+P VT+ G+ AGA SV L L S + LF + I++SG+P + V+ +
Sbjct: 342 DNIHRFGGDPSRVTLFGESAGAVSVSLHLLSALSRDLFQRAILQSGSPTAPWALVSREEA 401
Query: 242 A--AIKLAVHLGL--NTTETEEAIQFLTKTSPDLVVAAAKELELQLKPCRERSFSGISN- 296
A++LA +G ++ +A++ L P ++V E E F + +
Sbjct: 402 TLRALRLAEAVGCPHEPSKLSDAVECLRGKDPHVLV----NNEWGTLGICEFPFVPVVDG 457
Query: 297 -FVESDPYSLTNKKKVRNTPIMIGNTNKEKKSLSADYFSTDPFYEKLKSNFNLDE----- 350
F++ P + + T I+ G +N E+ Y+ T+ ++ +E
Sbjct: 458 AFLDETPQRSLASGRFKKTEILTG-SNTEEGYYFIIYYLTELLRKEEGVTVTREEFLQAV 516
Query: 351 ----AQIEKAAKNVRHFYIGDSSLSAEVASE---LQDFESDFVYNHPSQRTITQLLDENA 403
+ AA+ F D + S L D+ + + Q E
Sbjct: 517 RELNPYVNGAARQAIVFEYTDWTEPDNPNSNRDALDKMVGDYHFT-CNVNEFAQRYAEEG 575
Query: 404 GKIYEYLFSYSGELE-----DGAEHSSELNYLF-ETAGGS-DRTEEEQLMVDRITTLWTN 456
+Y YL+++ + G H E+NY+F E + TE+E+ +I W+N
Sbjct: 576 NNVYMYLYTHRSKGNPWPRWTGVMHGDEINYVFGEPLNPTLGYTEDEKDFSRKIMRYWSN 635
Query: 457 FVKFGNPTPKSTDLIPTIWEPVTADTRPVLVLDKDVRLDSRIERQRM-AYW 506
F K GNP P + W TA R L L + R R R A+W
Sbjct: 636 FAKTGNPNPNTASSEFPEWPKHTAHGRHYLELGLNTSFVGRGPRLRQCAFW 686
>AJ515150-1|CAD56157.2| 737|Anopheles gambiae acetylcholinesterase
protein.
Length = 737
Score = 132 bits (320), Expect = 2e-32
Identities = 142/531 (26%), Positives = 230/531 (43%), Gaps = 55/531 (10%)
Query: 23 DPLVLISNQGLVRGHK--AADGD-YSIFLGIPYAKVDVN-----NP---------FSTAT 65
DPLV+ +++G +RG A G ++LGIPYA+ V +P +T T
Sbjct: 164 DPLVVNTDKGRIRGITVDAPSGKKVDVWLGIPYAQPPVGPLRFRHPRPAEKWTGVLNTTT 223
Query: 66 DPIAFPEIIHDAVDEVRCPQTSSSVGEQTLNCLRLNIYVPSAASSRNPMPVLVWIHGGDF 125
P + +I+ + + + +CL +N+ P V++WI GG F
Sbjct: 224 PPNSCVQIVDTVFGDFPGATMWNPNTPLSEDCLYINVVAPRPRPKN--AAVMLWIFGGGF 281
Query: 126 ATGSAS--DYGVKNIV-RHGVLVVTMNYRLGPYGFLCLDVPTASGNQGLRDQYKALSWIR 182
+G+A+ Y + + V+VV++ YR+ GFL L P A GN GL DQ AL W+R
Sbjct: 282 YSGTATLDVYDHRALASEENVIVVSLQYRVASLGFLFLGTPEAPGNAGLFDQNLALRWVR 341
Query: 183 NNIASFGGNPYNVTIAGQDAGATSVLLQLYSD-NEKLFHKVIIESGTPQSEGMFVNADVE 241
+NI FGG+P VT+ G+ AGA SV L L S + LF + I++SG+P + V+ +
Sbjct: 342 DNIHRFGGDPSRVTLFGESAGAVSVSLHLLSALSRDLFQRAILQSGSPTAPWALVSREEA 401
Query: 242 A--AIKLAVHLGL--NTTETEEAIQFLTKTSPDLVVAAAKELELQLKPCRERSFSGISN- 296
A++LA +G ++ +A++ L P ++V E E F + +
Sbjct: 402 TLRALRLAEAVGCPHEPSKLSDAVECLRGKDPHVLV----NNEWGTLGICEFPFVPVVDG 457
Query: 297 -FVESDPYSLTNKKKVRNTPIMIGNTNKEKKSLSADYFSTDPFYEKLKSNFNLDE----- 350
F++ P + + T I+ G +N E+ Y+ T+ ++ +E
Sbjct: 458 AFLDETPQRSLASGRFKKTEILTG-SNTEEGYYFIIYYLTELLRKEEGVTVTREEFLQAV 516
Query: 351 ----AQIEKAAKNVRHFYIGDSSLSAEVASE---LQDFESDFVYNHPSQRTITQLLDENA 403
+ AA+ F D + S L D+ + + Q E
Sbjct: 517 RELNPYVNGAARQAIVFEYTDWTEPDNPNSNRDALDKMVGDYHFT-CNVNEFAQRYAEEG 575
Query: 404 GKIYEYLFSYSGELE-----DGAEHSSELNYLF-ETAGGS-DRTEEEQLMVDRITTLWTN 456
+Y YL+++ + G H E+NY+F E + TE+E+ +I W+N
Sbjct: 576 NNVYMYLYTHRSKGNPWPRWTGVMHGDEINYVFGEPLNPTLGYTEDEKDFSRKIMRYWSN 635
Query: 457 FVKFGNPTPKSTDLIPTIWEPVTADTRPVLVLDKDVRLDSRIERQRM-AYW 506
F K GNP P + W TA R L L + R R R A+W
Sbjct: 636 FAKTGNPNPNTASSEFPEWPKHTAHGRHYLELGLNTSFVGRGPRLRQCAFW 686
>AJ488492-1|CAD32684.2| 623|Anopheles gambiae acetylcholinesterase
protein.
Length = 623
Score = 132 bits (320), Expect = 2e-32
Identities = 142/531 (26%), Positives = 230/531 (43%), Gaps = 55/531 (10%)
Query: 23 DPLVLISNQGLVRGHK--AADGD-YSIFLGIPYAKVDVN-----NP---------FSTAT 65
DPLV+ +++G +RG A G ++LGIPYA+ V +P +T T
Sbjct: 50 DPLVVNTDKGRIRGITVDAPSGKKVDVWLGIPYAQPPVGPLRFRHPRPAEKWTGVLNTTT 109
Query: 66 DPIAFPEIIHDAVDEVRCPQTSSSVGEQTLNCLRLNIYVPSAASSRNPMPVLVWIHGGDF 125
P + +I+ + + + +CL +N+ P V++WI GG F
Sbjct: 110 PPNSCVQIVDTVFGDFPGATMWNPNTPLSEDCLYINVVAPRPRPKN--AAVMLWIFGGGF 167
Query: 126 ATGSAS--DYGVKNIV-RHGVLVVTMNYRLGPYGFLCLDVPTASGNQGLRDQYKALSWIR 182
+G+A+ Y + + V+VV++ YR+ GFL L P A GN GL DQ AL W+R
Sbjct: 168 YSGTATLDVYDHRALASEENVIVVSLQYRVASLGFLFLGTPEAPGNAGLFDQNLALRWVR 227
Query: 183 NNIASFGGNPYNVTIAGQDAGATSVLLQLYSD-NEKLFHKVIIESGTPQSEGMFVNADVE 241
+NI FGG+P VT+ G+ AGA SV L L S + LF + I++SG+P + V+ +
Sbjct: 228 DNIHRFGGDPSRVTLFGESAGAVSVSLHLLSALSRDLFQRAILQSGSPTAPWALVSREEA 287
Query: 242 A--AIKLAVHLGL--NTTETEEAIQFLTKTSPDLVVAAAKELELQLKPCRERSFSGISN- 296
A++LA +G ++ +A++ L P ++V E E F + +
Sbjct: 288 TLRALRLAEAVGCPHEPSKLSDAVECLRGKDPHVLV----NNEWGTLGICEFPFVPVVDG 343
Query: 297 -FVESDPYSLTNKKKVRNTPIMIGNTNKEKKSLSADYFSTDPFYEKLKSNFNLDE----- 350
F++ P + + T I+ G +N E+ Y+ T+ ++ +E
Sbjct: 344 AFLDETPQRSLASGRFKKTEILTG-SNTEEGYYFIIYYLTELLRKEEGVTVTREEFLQAV 402
Query: 351 ----AQIEKAAKNVRHFYIGDSSLSAEVASE---LQDFESDFVYNHPSQRTITQLLDENA 403
+ AA+ F D + S L D+ + + Q E
Sbjct: 403 RELNPYVNGAARQAIVFEYTDWTEPDNPNSNRDALDKMVGDYHFT-CNVNEFAQRYAEEG 461
Query: 404 GKIYEYLFSYSGELE-----DGAEHSSELNYLF-ETAGGS-DRTEEEQLMVDRITTLWTN 456
+Y YL+++ + G H E+NY+F E + TE+E+ +I W+N
Sbjct: 462 NNVYMYLYTHRSKGNPWPRWTGVMHGDEINYVFGEPLNPTLGYTEDEKDFSRKIMRYWSN 521
Query: 457 FVKFGNPTPKSTDLIPTIWEPVTADTRPVLVLDKDVRLDSRIERQRM-AYW 506
F K GNP P + W TA R L L + R R R A+W
Sbjct: 522 FAKTGNPNPNTASSEFPEWPKHTAHGRHYLELGLNTSFVGRGPRLRQCAFW 572
>AJ535204-1|CAD59404.1| 1187|Anopheles gambiae SMC2 protein protein.
Length = 1187
Score = 28.7 bits (61), Expect = 0.41
Identities = 24/86 (27%), Positives = 35/86 (40%), Gaps = 5/86 (5%)
Query: 247 AVHLGLNTTETEEAIQF-----LTKTSPDLVVAAAKELELQLKPCRERSFSGISNFVESD 301
AV GL+T E EA K A K+ E++LK ++ N SD
Sbjct: 382 AVSAGLSTNEDGEAATLQDQLIAAKQKSAEATTAIKQSEMELKHSQQLLRDKQKNMNSSD 441
Query: 302 PYSLTNKKKVRNTPIMIGNTNKEKKS 327
L +K+K+ IG +E +S
Sbjct: 442 AAYLEDKRKLTKVEGQIGQLERELQS 467
>AJ439398-8|CAD28131.1| 1283|Anopheles gambiae putative
differentiation regulator protein.
Length = 1283
Score = 25.8 bits (54), Expect = 2.9
Identities = 17/43 (39%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
Query: 166 SGNQGLRDQY--KALSWIRNNIASFGGNPYNVTIAGQDAGATS 206
S N G D++ KA + N A GG N AG D GA S
Sbjct: 1109 SENNGTLDKHHEKAATVNSNGNAGSGGGQANQAAAGSDGGAGS 1151
>AY146716-1|AAO12076.1| 159|Anopheles gambiae odorant-binding
protein AgamOBP12 protein.
Length = 159
Score = 25.0 bits (52), Expect = 5.0
Identities = 13/44 (29%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Query: 333 FSTDPFYEKLKSNFNLDEAQIEKAAKN-VRHFYIGDSSLSAEVA 375
F D YE++K ++DE ++ KN +R+ G + + E A
Sbjct: 90 FDVDKQYERVKDRLSVDEDTYKRGVKNCIRNVLRGRTLNNCEKA 133
>L04753-1|AAA29357.1| 511|Anopheles gambiae alpha-amylase protein.
Length = 511
Score = 24.6 bits (51), Expect = 6.7
Identities = 9/30 (30%), Positives = 17/30 (56%)
Query: 170 GLRDQYKALSWIRNNIASFGGNPYNVTIAG 199
GL D +A+ W+R+ + F + + +AG
Sbjct: 182 GLHDLNQAVPWVRDRVVDFLNHLIELGVAG 211
>AJ535203-1|CAD59403.1| 1229|Anopheles gambiae SMC1 protein protein.
Length = 1229
Score = 24.6 bits (51), Expect = 6.7
Identities = 18/87 (20%), Positives = 35/87 (40%), Gaps = 3/87 (3%)
Query: 299 ESDPYSLTNKKKVRNTPIMIGNTNKEKKSLSADYFSTDPFYEKLKSNFNLDEAQIEKAAK 358
E+D KK+V + +E + + A+ P + K K + +++ A K
Sbjct: 268 EADEVLKEKKKEVGKMTREMAKKEQEIREVEAEMSKRHPMFIKAKEKVAHTQKKLDGALK 327
Query: 359 NVRHFYIGDSSLSAEV---ASELQDFE 382
+ D + A++ ELQ+ E
Sbjct: 328 TLEQARRADEAHQADIKKLVDELQEVE 354
Database: mosquito
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 516,269
Number of sequences in database: 2123
Lambda K H
0.317 0.134 0.394
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 556,606
Number of Sequences: 2123
Number of extensions: 22755
Number of successful extensions: 78
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 56
Number of HSP's gapped (non-prelim): 14
length of query: 531
length of database: 516,269
effective HSP length: 67
effective length of query: 464
effective length of database: 374,028
effective search space: 173548992
effective search space used: 173548992
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 50 (24.2 bits)
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