Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000866-TA|BGIBMGA000866-PA|IPR000246|Peptidase T2,
asparaginase 2
(248 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine... 188 4e-48
At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine... 188 4e-48
At3g16150.1 68416.m02039 L-asparaginase, putative / L-asparagine... 67 1e-11
At5g08100.2 68418.m00944 L-asparaginase / L-asparagine amidohydr... 58 6e-09
At5g08100.1 68418.m00945 L-asparaginase / L-asparagine amidohydr... 58 6e-09
At4g00590.1 68417.m00083 asparaginase 2 family protein very low ... 44 1e-04
At1g62300.1 68414.m07028 WRKY family transcription factor simila... 29 3.9
At2g25530.1 68415.m03056 AFG1-like ATPase family protein contain... 27 9.0
>At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 257
Score = 188 bits (457), Expect = 4e-48
Identities = 106/259 (40%), Positives = 145/259 (55%), Gaps = 18/259 (6%)
Query: 1 MNIGAVGGLRRIKHAISVARHVLDHTKHSFLVGELATQFAVEMGFQEE-SLTTSVSKKLW 59
M +GAV +R +K I A V+ +++H+ L GE A+ FA+ MG +L++ S K W
Sbjct: 1 MEVGAVAAMRYVKDGIRAAHLVMKYSQHTLLAGEGASAFAISMGLPGPMNLSSPESVKKW 60
Query: 60 MKWHNEHHCQPNFWLNVSPDPTRNCGPYQKHEYSHHL-LKKSHLN------------VDR 106
W E+ CQPNF NV P +CGPY+ + + ++ + KS + V
Sbjct: 61 SDW-KENQCQPNFRKNVVP--ANDCGPYKPNNSAMNVFVDKSTESCEMGAIEYKPPLVGP 117
Query: 107 FNHDTIGMVAIDQHGDIAAGTSTNGAKYKIPGRIGDSPIPGSGAYXXXXXXXXXXXXXXX 166
NHDTI M ID+ G IA GTSTNGA YKIPGR+GD PI GS AY
Sbjct: 118 HNHDTISMAVIDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGD 177
Query: 167 VMLRFLPSFLTVEEMRRGSSPTDAAKTAVARIASHYPDFMGAVVALKNDGEYGAACHGIN 226
M+RFLP + VE MR+G P +AAK A++RIA +PDF+GAVVA+ +G + AC+G
Sbjct: 178 TMMRFLPCYQVVESMRQGMKPEEAAKDAISRIARKFPDFVGAVVAVDKNGSHAGACYGWT 237
Query: 227 MFPYVVYDKKHSSYQIVNV 245
F Y V + + Q+ V
Sbjct: 238 -FQYSVQNPDMNDVQVFTV 255
>At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 359
Score = 188 bits (457), Expect = 4e-48
Identities = 106/259 (40%), Positives = 145/259 (55%), Gaps = 18/259 (6%)
Query: 1 MNIGAVGGLRRIKHAISVARHVLDHTKHSFLVGELATQFAVEMGFQEE-SLTTSVSKKLW 59
M +GAV +R +K I A V+ +++H+ L GE A+ FA+ MG +L++ S K W
Sbjct: 103 MEVGAVAAMRYVKDGIRAAHLVMKYSQHTLLAGEGASAFAISMGLPGPMNLSSPESVKKW 162
Query: 60 MKWHNEHHCQPNFWLNVSPDPTRNCGPYQKHEYSHHL-LKKSHLN------------VDR 106
W E+ CQPNF NV P +CGPY+ + + ++ + KS + V
Sbjct: 163 SDW-KENQCQPNFRKNVVP--ANDCGPYKPNNSAMNVFVDKSTESCEMGAIEYKPPLVGP 219
Query: 107 FNHDTIGMVAIDQHGDIAAGTSTNGAKYKIPGRIGDSPIPGSGAYXXXXXXXXXXXXXXX 166
NHDTI M ID+ G IA GTSTNGA YKIPGR+GD PI GS AY
Sbjct: 220 HNHDTISMAVIDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGD 279
Query: 167 VMLRFLPSFLTVEEMRRGSSPTDAAKTAVARIASHYPDFMGAVVALKNDGEYGAACHGIN 226
M+RFLP + VE MR+G P +AAK A++RIA +PDF+GAVVA+ +G + AC+G
Sbjct: 280 TMMRFLPCYQVVESMRQGMKPEEAAKDAISRIARKFPDFVGAVVAVDKNGSHAGACYGWT 339
Query: 227 MFPYVVYDKKHSSYQIVNV 245
F Y V + + Q+ V
Sbjct: 340 -FQYSVQNPDMNDVQVFTV 357
>At3g16150.1 68416.m02039 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:P30364
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Lupinus angustifolius]
Length = 325
Score = 66.9 bits (156), Expect = 1e-11
Identities = 50/151 (33%), Positives = 74/151 (49%), Gaps = 11/151 (7%)
Query: 4 GAVGGLRRIKHAISVARHVLDHTKHSFLVGELATQFAVEMGFQ---EESLTTSVSKKLWM 60
GAV G+ +K+ IS+AR V+D + HS+L A FA + G + E T + + +
Sbjct: 93 GAVSGITTVKNPISLARLVMDKSPHSYLAFSGAEDFARKQGVEIVDNEYFVTDDNVGM-L 151
Query: 61 KWHNEHHCQPNFWLNVSPDPTRNCGPYQKHEYSHHLLKKSHLNVDRFNHDTIGMVAIDQH 120
K E N L P C + ++ + L + + +T+G V +D
Sbjct: 152 KLAKE----ANSILFDYRIPPMGCAGAAATDSP---IQMNGLPISIYAPETVGCVVVDGK 204
Query: 121 GDIAAGTSTNGAKYKIPGRIGDSPIPGSGAY 151
G AAGTST G K+ GRIGDSP+ G+G Y
Sbjct: 205 GHCAAGTSTGGLMNKMMGRIGDSPLIGAGTY 235
>At5g08100.2 68418.m00944 L-asparaginase / L-asparagine
amidohydrolase identical to Swiss-Prot:P50287
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Arabidopsis thaliana]
Length = 235
Score = 58.0 bits (134), Expect = 6e-09
Identities = 46/150 (30%), Positives = 70/150 (46%), Gaps = 22/150 (14%)
Query: 4 GAVGGLRRIKHAISVARHVLDHTKHSFLVGELATQFAVEMGFQEESLTTSVSKK--LWMK 61
GAV GL + + IS+AR V++ T H +L + A FA G + + ++ + +K
Sbjct: 14 GAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDSSHFITPENIARLK 73
Query: 62 WHNEHHCQPNFWLNVSPDPTRNCGPYQKHEYSHHLLKKSHLNVDRFNHDTIGMVAIDQHG 121
E + + SP NCG Q T+G VA+D G
Sbjct: 74 QAKEFNRVQLDYTVPSPKVPDNCGDSQI--------------------GTVGCVAVDSAG 113
Query: 122 DIAAGTSTNGAKYKIPGRIGDSPIPGSGAY 151
++A+ TST G K+ GRIGD+P+ G+G Y
Sbjct: 114 NLASATSTGGYVNKMVGRIGDTPVIGAGTY 143
>At5g08100.1 68418.m00945 L-asparaginase / L-asparagine
amidohydrolase identical to Swiss-Prot:P50287
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Arabidopsis thaliana]
Length = 315
Score = 58.0 bits (134), Expect = 6e-09
Identities = 46/150 (30%), Positives = 70/150 (46%), Gaps = 22/150 (14%)
Query: 4 GAVGGLRRIKHAISVARHVLDHTKHSFLVGELATQFAVEMGFQEESLTTSVSKK--LWMK 61
GAV GL + + IS+AR V++ T H +L + A FA G + + ++ + +K
Sbjct: 94 GAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDSSHFITPENIARLK 153
Query: 62 WHNEHHCQPNFWLNVSPDPTRNCGPYQKHEYSHHLLKKSHLNVDRFNHDTIGMVAIDQHG 121
E + + SP NCG Q T+G VA+D G
Sbjct: 154 QAKEFNRVQLDYTVPSPKVPDNCGDSQI--------------------GTVGCVAVDSAG 193
Query: 122 DIAAGTSTNGAKYKIPGRIGDSPIPGSGAY 151
++A+ TST G K+ GRIGD+P+ G+G Y
Sbjct: 194 NLASATSTGGYVNKMVGRIGDTPVIGAGTY 223
>At4g00590.1 68417.m00083 asparaginase 2 family protein very low
similarity to glycosylasparaginase [SP|P20933] from Homo
sapiens; contains Pfam profile PF01112: Asparaginase 2
Length = 408
Score = 44.0 bits (99), Expect = 1e-04
Identities = 18/42 (42%), Positives = 27/42 (64%)
Query: 110 DTIGMVAIDQHGDIAAGTSTNGAKYKIPGRIGDSPIPGSGAY 151
DT+G++ +D G IA G+S+ G KI GR+G + GSG +
Sbjct: 221 DTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCW 262
>At1g62300.1 68414.m07028 WRKY family transcription factor similar
to putative DNA-binding protein GI:7268215 from
[Arabidopsis thaliana]
Length = 553
Score = 28.7 bits (61), Expect = 3.9
Identities = 14/38 (36%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Query: 100 SHLNVDRFNHDTIGMVAIDQHGDIAAGTSTNGAKYKIP 137
SH N RF++ + M ID+ D STNG++++ P
Sbjct: 24 SHKNHRRFSNP-LAMSRIDEEDDQKTRISTNGSEFRFP 60
>At2g25530.1 68415.m03056 AFG1-like ATPase family protein contains
Pfam profile: PF03969 AFG1-like ATPase
Length = 655
Score = 27.5 bits (58), Expect = 9.0
Identities = 12/54 (22%), Positives = 20/54 (37%)
Query: 57 KLWMKWHNEHHCQPNFWLNVSPDPTRNCGPYQKHEYSHHLLKKSHLNVDRFNHD 110
++W WH + D + Y K HHL + ++ R NH+
Sbjct: 8 RVWQIWHPSFDISTKPTKEIYTDTNKPNQQYDKRSIFHHLNRDRKASLQRRNHN 61
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.320 0.134 0.417
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,884,750
Number of Sequences: 28952
Number of extensions: 232268
Number of successful extensions: 410
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 395
Number of HSP's gapped (non-prelim): 14
length of query: 248
length of database: 12,070,560
effective HSP length: 79
effective length of query: 169
effective length of database: 9,783,352
effective search space: 1653386488
effective search space used: 1653386488
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 58 (27.5 bits)
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