BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000851-TA|BGIBMGA000851-PA|IPR009080|Aminoacyl-tRNA synthetase, class 1a, anticodon-binding, IPR001278|Arginyl-tRNA synthetase, class Ic, IPR008909|DALR anticodon binding (365 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC25B2.09c |||arginine-tRNA ligase|Schizosaccharomyces pombe|c... 224 1e-59 SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 29 1.0 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 27 3.2 SPAC343.12 |rds1||conserved fungal protein|Schizosaccharomyces p... 27 4.2 SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 27 4.2 SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr... 27 5.5 SPBC31F10.03 |||ChaC-like protein|Schizosaccharomyces pombe|chr ... 26 7.3 SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 26 7.3 SPAC3G9.08 |png1||ING family homolog Png1|Schizosaccharomyces po... 26 9.6 SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subuni... 26 9.6 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 26 9.6 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 26 9.6 SPAC1610.04 |mug99||meiotically upregulated gene Mug99|Schizosac... 26 9.6 >SPBC25B2.09c |||arginine-tRNA ligase|Schizosaccharomyces pombe|chr 2|||Manual Length = 618 Score = 224 bits (548), Expect = 1e-59 Identities = 121/364 (33%), Positives = 205/364 (56%), Gaps = 8/364 (2%) Query: 10 NVQNEARKYFSDIEQG-KISLENWKKIRNVTVHELENVYQRLGITFDAFHWESDYNGIAI 68 ++ ++AR++F +E G + SL+ W + R++++ +L++ Y RL I +D + ES + + Sbjct: 255 SLHDKAREFFKRMEDGDEESLKVWARFRDLSITKLKDTYDRLNIHYDEYDGESQVSLELM 314 Query: 69 RDLMDYLQKHNIISNDETGXXXXXXXXXX----XXFLKSDNSTLYLTRDVAALLDRHKKY 124 ++D L+ N+I D K D +TLYLTRD+ R++KY Sbjct: 315 NKMVDELRSLNLIEEDGGALLIDLSKHDKKLGKAIVQKRDGTTLYLTRDIGTAYKRYEKY 374 Query: 125 DFDKMLYIVDNSQADHFSSVFEIVKQFNKKCTEGCEHIKFGRIKGMSTRKGNAVFLNDIL 184 FDK +Y+V + Q +FS +F+I + + C HI +G ++GMSTRKG AVFL+DI+ Sbjct: 375 KFDKSIYVVSSQQDMYFSQLFKIFELMGFDWAKKCVHINYGLVQGMSTRKGKAVFLDDIM 434 Query: 185 DEAKQKMYEKQMQSKNTRQSAMN-EETCDILGTTAVVINDLKQRRQRDYEFNWDRALQSE 243 + AK++M++ +++ N EE DI+G TA+ I D +R +Y F+W R E Sbjct: 435 EVAKEEMHKVMQKNEEKYAQVENPEEVADIVGKTAIRIQDSTGKRINNYAFDWSRMTSFE 494 Query: 244 GDSGIKLQYLHCRLRSLEKNCGVSIPEF--CSPALLTEDIIVDVVNELSKFENVLQRSFE 301 GD+G LQY H RL S+ +N + E + LLTE D+V L ++ +VL+ +F Sbjct: 495 GDTGPYLQYAHSRLSSVRRNVNYTDEEIMGANLELLTEPDAYDLVRLLGQYPDVLKNAFR 554 Query: 302 AYEACILVSYLFRLAKCINKMFKEMKVKNVDQSTAEQRLLVFHAARIVVKTSLEILGVRP 361 E +V+YLF+L ++K++ + V+ ++ RL +F AA+ V+ + +LG+ P Sbjct: 555 FQETSTVVTYLFKLTHAVSKLYDILWVRGRERDIQLARLALFGAAKQVLNNGMTLLGLTP 614 Query: 362 LDEM 365 L+ M Sbjct: 615 LERM 618 >SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1639 Score = 29.1 bits (62), Expect = 1.0 Identities = 19/59 (32%), Positives = 29/59 (49%), Gaps = 1/59 (1%) Query: 160 EHIKFGRIKGMSTRKGNAVFLNDILDEAKQKMYEKQMQSKNTRQSAMNEETCDILGTTA 218 E + + RI S K +NDILD +K + ++ S+ AM E+ + LGT A Sbjct: 1027 EQLSYARIIQQSA-KSLLTVINDILDLSKVRAGMMKLTSQRFSVRAMMEDANETLGTLA 1084 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 27.5 bits (58), Expect = 3.2 Identities = 12/53 (22%), Positives = 26/53 (49%), Gaps = 2/53 (3%) Query: 9 ENVQNEARKYFSDIEQGKISLENWKKIRNVTVHELENVYQRLGITFDAFHWES 61 EN + ++ + E+ E W+++++V + + Y +G F+ WES Sbjct: 1826 ENAADSCVRHLENNEETDWKFEEWRQMQDVVKQDQDRSY--IGFHFENSKWES 1876 >SPAC343.12 |rds1||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 27.1 bits (57), Expect = 4.2 Identities = 16/58 (27%), Positives = 30/58 (51%), Gaps = 1/58 (1%) Query: 140 HFSSVFEIVKQFNKKCTEGCEHIKFGRIKGMSTRKGNAVFLNDILDEAKQKMYEKQMQ 197 +F +V + F + T+ E +G + + +R A+ L I EA+Q+M +Q+Q Sbjct: 152 NFDTVGSFI-DFAQTLTKWSESGVYGFLPHLDSRAAAALLLQSITTEARQQMSLRQLQ 208 >SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 1052 Score = 27.1 bits (57), Expect = 4.2 Identities = 12/35 (34%), Positives = 20/35 (57%), Gaps = 2/35 (5%) Query: 185 DEAKQKMYEKQMQSKNTRQSAMNEETCDILGTTAV 219 D+ + K+YE QM KN+ +N C+ + TA+ Sbjct: 835 DDCENKVYEGQMNDKNSSLDMVN--ACEGINQTAI 867 >SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr 1|||Manual Length = 872 Score = 26.6 bits (56), Expect = 5.5 Identities = 14/47 (29%), Positives = 26/47 (55%), Gaps = 2/47 (4%) Query: 271 FCSPALLTEDIIVDVVNELSKFENVLQRSFEAYEACILVSYLFRLAK 317 FC+ + +I+D N+L K N+L+ S+E +L+ + R+ K Sbjct: 319 FCTNFPNAQQLIIDNQNQLLKVANLLEPSYEL--PALLIKPIQRICK 363 >SPBC31F10.03 |||ChaC-like protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 203 Score = 26.2 bits (55), Expect = 7.3 Identities = 13/29 (44%), Positives = 17/29 (58%) Query: 311 YLFRLAKCINKMFKEMKVKNVDQSTAEQR 339 YLF LAKC+ + E K +V + AE R Sbjct: 168 YLFELAKCLRHLSPESKDIHVFELEAEVR 196 >SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 3655 Score = 26.2 bits (55), Expect = 7.3 Identities = 13/45 (28%), Positives = 21/45 (46%) Query: 190 KMYEKQMQSKNTRQSAMNEETCDILGTTAVVINDLKQRRQRDYEF 234 K+Y +Q TR S E +LGT V+ + + R++ F Sbjct: 3309 KLYPFALQYPATRHSRREERMLQLLGTFNTVLRSKIEIQNRNFSF 3353 >SPAC3G9.08 |png1||ING family homolog Png1|Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 25.8 bits (54), Expect = 9.6 Identities = 10/37 (27%), Positives = 24/37 (64%), Gaps = 1/37 (2%) Query: 1 MNKLAETDENVQNEARKYFSDIEQGKISLEN-WKKIR 36 +++ +T +NV NE + F +I ++++ + WK+I+ Sbjct: 9 LSEYLQTLDNVPNETKHIFDEISVKEVAVHDIWKRIQ 45 >SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subunit 8|Schizosaccharomyces pombe|chr 3|||Manual Length = 230 Score = 25.8 bits (54), Expect = 9.6 Identities = 16/46 (34%), Positives = 26/46 (56%), Gaps = 3/46 (6%) Query: 188 KQKMYEKQMQSKNTRQSAMNEE---TCDILGTTAVVINDLKQRRQR 230 +QK EK+ K+ R+++ NEE D+ G + LKQ++QR Sbjct: 135 QQKKKEKRAALKHYREASDNEEDLKETDLYGDRDSFQSRLKQKKQR 180 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 25.8 bits (54), Expect = 9.6 Identities = 10/27 (37%), Positives = 16/27 (59%) Query: 46 VYQRLGITFDAFHWESDYNGIAIRDLM 72 VY RLG+ A +W++ G+ I D + Sbjct: 710 VYWRLGVQMVALNWQTYDTGLQINDAL 736 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 25.8 bits (54), Expect = 9.6 Identities = 20/70 (28%), Positives = 32/70 (45%), Gaps = 6/70 (8%) Query: 3 KLAETDENVQ-NEARKYFSDIEQGKISLENWKKI-----RNVTVHELENVYQRLGITFDA 56 K+ E D + A + F D E GKISL N +++ N+ ELE + + + D Sbjct: 102 KIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEEFDLDQDG 161 Query: 57 FHWESDYNGI 66 E ++ I Sbjct: 162 EINEQEFIAI 171 >SPAC1610.04 |mug99||meiotically upregulated gene Mug99|Schizosaccharomyces pombe|chr 1|||Manual Length = 526 Score = 25.8 bits (54), Expect = 9.6 Identities = 16/58 (27%), Positives = 28/58 (48%), Gaps = 4/58 (6%) Query: 27 ISLENWKKIRNVTVHELENVYQRLGITFDAFHWESDYNGI--AIRDLM--DYLQKHNI 80 IS+E W K +V + E E Q+ F S+ + +++ L+ +YLQK + Sbjct: 293 ISIEKWAKYCDVDLQEFEKKLQKFWKNFGTLKLYSNIESLPASLKQLIKDEYLQKSKL 350 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.320 0.134 0.385 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,589,078 Number of Sequences: 5004 Number of extensions: 65417 Number of successful extensions: 216 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 204 Number of HSP's gapped (non-prelim): 16 length of query: 365 length of database: 2,362,478 effective HSP length: 74 effective length of query: 291 effective length of database: 1,992,182 effective search space: 579724962 effective search space used: 579724962 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 54 (25.8 bits)
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