Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000846-TA|BGIBMGA000846-PA|IPR001683|Phox-like,
IPR001736|Phospholipase D/Transphosphatidylase
(1199 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr 1|... 255 2e-68
SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protei... 33 0.24
SPBC13E7.06 |msd1|mug172|spindle pole body protein Msd1|Schizosa... 31 1.3
SPAC26A3.03c |rmi1||RecQ mediated genome instability protein Rmi... 30 1.7
SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 29 3.9
SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces... 29 5.2
SPBC215.14c |vps20||vacuolar sorting protein Vps20|Schizosacchar... 28 6.8
SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 28 6.8
SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 28 6.8
SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub... 28 9.0
>SPAC2F7.16c |||phospholipase D |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1369
Score = 255 bits (625), Expect = 2e-68
Identities = 135/301 (44%), Positives = 188/301 (62%), Gaps = 16/301 (5%)
Query: 749 WIGKDYTNFIVKDFNNLDLPFVDLVDRNTTPRMPWHDVGLVVQGAAARDVARHFIQRWN- 807
W GKDY+N V DF +L P+ D+ DR PRM WHDV + + G ARD ARHF+QRWN
Sbjct: 693 WRGKDYSNARVHDFFDLTEPYKDMYDRLAVPRMGWHDVSMCIIGQPARDAARHFVQRWNY 752
Query: 808 AIKLEKARQNTNYPYLVPKTYSDIQPLGDFDKLLNIDMNNV-SCQVLRSVSSWSGGFLDP 866
I+ +K + T P L+P P D+L + + QVLRS WS G +
Sbjct: 753 LIQCKKPARKT--PLLIP------PPDFTTDQLTDSQLTGTCEVQVLRSAGLWSLGLV-- 802
Query: 867 DTVEQSIHEAYVDTITRAQHYLYIENQFFITLSR-SSVAVRNQIGEALFNRIMRAHRGGE 925
DTVEQSI AYV I +++H++YIENQFF+T + + N++G+AL RI+RAH+ E
Sbjct: 803 DTVEQSIQNAYVTCIEKSEHFIYIENQFFVTSTTCEGTTIENRVGDALVERIIRAHKNNE 862
Query: 926 AFRVYVVMPLLPAFEGEVGAPSGTSLHAVTHWNYQSISRSREAILTRLYEAGVSDPSEYI 985
+R +++PLLP FEG++ G SL + Y+SI +I RL G+ D S+Y+
Sbjct: 863 KWRGVIMIPLLPGFEGQIDLQEGGSLRLIVECQYRSICHGEHSIFGRLNAKGI-DGSKYL 921
Query: 986 TFHGLR--THSRLEGEPVTELIYVHSKLLIADDKTVICGSANLNDRSMLGSRDSEIAVLL 1043
F+GLR H E VTE+IYVH+K+LIADD+ + GSAN+N+RS+LG+RDSEIA ++
Sbjct: 922 RFYGLRGWAHLGENHELVTEMIYVHAKILIADDRVAVIGSANINERSLLGNRDSEIAAVI 981
Query: 1044 Q 1044
+
Sbjct: 982 R 982
Score = 144 bits (348), Expect = 9e-35
Identities = 70/205 (34%), Positives = 118/205 (57%), Gaps = 8/205 (3%)
Query: 345 NHGLQILNQSRQMVIKCWTKRKSKEWMNYLKTVANQSARDFTYPNVHH--SFXXXXXXXX 402
+H +I N+ + M + + R ++++N ++ +A + +H SF
Sbjct: 478 HHSFKIKNRQKVMKLSVRSGRWLQQFINSVQVAQGLTA----WCEIHRFDSFAPVRTNVA 533
Query: 403 XXXLVDGAEYFSAAADAMELAREEIFIADWWLSPEVYMKRPALNGNYWRLDMILKRKAAQ 462
+VD ++ + A++ A+ I I WWLSPE+ M+RP + WR+D IL KA +
Sbjct: 534 VQWMVDARDHMWNVSRAIKNAKRCIMIHGWWLSPELQMRRPYSMAHKWRIDRILNEKAHE 593
Query: 463 GVKIFILLYKEVEMALGINSYYSKSRLAN--DNIKVFRHPDHAKAGVFFWAHHEKIVVVD 520
GV ++I++Y+ ++ + I+S+++K L + NI V R P H + FWAHHEK+VVVD
Sbjct: 594 GVMVYIMIYRNIDATIPIDSFHTKEHLQSLHPNIYVIRSPSHFRQNALFWAHHEKLVVVD 653
Query: 521 QSVAFLGGIDLCYGRWDDHRHRLTD 545
++ F+GGIDLC+GR+D +H L D
Sbjct: 654 DAITFIGGIDLCFGRYDTPQHILYD 678
Score = 43.6 bits (98), Expect = 2e-04
Identities = 14/47 (29%), Positives = 26/47 (55%)
Query: 1098 DDPCCERFYRHVWQAVSRQNTEIYEDVFHSIPTDAVHTFAQLKRYQE 1144
+DP C+ F+ +W V+ NT IY +F +P D + T+ +++
Sbjct: 1210 EDPVCDEFFEDIWSKVASNNTTIYRHIFRCVPDDEMLTWESYNEWKK 1256
>SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1462
Score = 33.1 bits (72), Expect = 0.24
Identities = 26/102 (25%), Positives = 47/102 (46%), Gaps = 6/102 (5%)
Query: 663 DRGKDIISSILFDEERNEHKFDESERKKAEKYARSNDSVL-LTDALGVRGAGGTARTPAP 721
+ GK+ + S +E ++H D++ K E A+ ++S+L +AL +G +
Sbjct: 910 EEGKNPVESNCSEESLDDHNIDQTLVNKKETLAQDSESLLQKNNALNEKGFENQFGLSSS 969
Query: 722 LAQVVEGRVITESTKDALEGVEGNSKLWIGKDYTNFIVKDFN 763
A +V+ + T D + G NS KD+T K+ N
Sbjct: 970 AA-----KVLNKDTLDHVSGPISNSVSSSFKDFTRTPFKEIN 1006
>SPBC13E7.06 |msd1|mug172|spindle pole body protein
Msd1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 331
Score = 30.7 bits (66), Expect = 1.3
Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Query: 812 EKARQNTNYPYLVPKTYSDIQPLGDFDKLLNIDMNNVSCQVLRSVSS 858
E +QNTN P L P+T S +Q + LN +NN Q+L+ +++
Sbjct: 179 EPIKQNTNAPIL-PETASFLQQNDENSNFLNSRVNNDEFQLLKGLNN 224
>SPAC26A3.03c |rmi1||RecQ mediated genome instability protein
Rmi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 235
Score = 30.3 bits (65), Expect = 1.7
Identities = 25/113 (22%), Positives = 51/113 (45%), Gaps = 1/113 (0%)
Query: 898 LSRSSVAVRNQIGEALFNRIMRAHRGGEAFRVYVVMPLLPAFE-GEVGAPSGTSLHAVTH 956
L+ V V+N+ ++L + + + H G +VM L A + E G + + V+
Sbjct: 11 LTELGVRVQNRWLQSLLDYLAKKHSTGANTTPQLVMQYLVASDIRESTTSEGAAPYIVSE 70
Query: 957 WNYQSISRSREAILTRLYEAGVSDPSEYITFHGLRTHSRLEGEPVTELIYVHS 1009
+ I + + R+ E G+S ++ + L +L+G+ V L++ S
Sbjct: 71 QHNVRIENTMLLQIVRVREIGISIVNQLEYLNDLEELKKLKGQKVIRLVHDES 123
>SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 376
Score = 29.1 bits (62), Expect = 3.9
Identities = 19/50 (38%), Positives = 25/50 (50%), Gaps = 2/50 (4%)
Query: 643 PDYRTDTPEIQKRNVLDKLTDRGKDIISSILFDEERNEHKFDESERKKAE 692
PD+RT + R+ +T R DI S L + N + DES RKK E
Sbjct: 269 PDFRTRNHQFHSRDS-QPITQRHTDI-ESRLQKMQDNAKELDESRRKKIE 316
>SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 745
Score = 28.7 bits (61), Expect = 5.2
Identities = 17/76 (22%), Positives = 33/76 (43%)
Query: 185 ITKPRKRRGALPRFPKKPEVMITYEGIQLRMKQLEEYLYNLLNISIYRNHHETVKFLEVS 244
+ KP F +KP + + E + R + YL N + + HET+ ++
Sbjct: 331 LIKPPPSNRVWYAFYEKPRLHLIVEPMVARKQLTNNYLINFITQKLVELVHETIVMPNMN 390
Query: 245 NLSFISELGSKGKEGM 260
+L+F + + K G+
Sbjct: 391 DLAFFIDNEAPIKGGL 406
>SPBC215.14c |vps20||vacuolar sorting protein
Vps20|Schizosaccharomyces pombe|chr 2|||Manual
Length = 226
Score = 28.3 bits (60), Expect = 6.8
Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 2/49 (4%)
Query: 5 TPTTPGDCLPGLFQHMDSLSPGLDSEFDESLAVPESLSVIDIDKVDNKD 53
TP+T D LP + + + P LD F ++ +V + S + ID+ KD
Sbjct: 176 TPSTGVDVLPSV--PLKNAIPSLDESFPKAASVSNTSSAVVIDEELRKD 222
>SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large
subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 1689
Score = 28.3 bits (60), Expect = 6.8
Identities = 13/20 (65%), Positives = 15/20 (75%)
Query: 480 INSYYSKSRLANDNIKVFRH 499
I+S YS SRL N N KV+RH
Sbjct: 577 ISSPYSYSRLINTNKKVYRH 596
>SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 611
Score = 28.3 bits (60), Expect = 6.8
Identities = 22/69 (31%), Positives = 36/69 (52%), Gaps = 8/69 (11%)
Query: 471 YKEVEMALGINSYYSKSRLAND-NIKVFRHPDHAKAGV---FFWAHHEKIVVVD----QS 522
YK++ MA GI +YY +R D +++ R P+ + + F HE + VV+ +
Sbjct: 218 YKQILMASGIGNYYQIARCFRDEDLRFDRQPEFTQIDLEMSFVDKPHEIMEVVEDLLVRL 277
Query: 523 VAFLGGIDL 531
V+F GI L
Sbjct: 278 VSFAKGITL 286
>SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase
Ubp21|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1129
Score = 27.9 bits (59), Expect = 9.0
Identities = 27/98 (27%), Positives = 43/98 (43%), Gaps = 6/98 (6%)
Query: 79 RKVFIPGVEIKVRFVENER---SVTTHLLNPNLYTISLQHGDFTWTIKKRYKHILNLHQQ 135
RK+ +P E V F+EN+ SVT +L T L H + KK ++ +Q
Sbjct: 179 RKIAVPTPEFPVPFLENDEICISVTVRVLQDP--TGVLWHSFVNYNSKKETGYVGLKNQG 236
Query: 136 LTLYRASLNIPFPTKAHKSRRASFKNTVDTEEKAERVA 173
T Y SL + + R+ +K D ++ + VA
Sbjct: 237 ATCYMNSL-LQSLFFTNIFRKTVYKIPTDNDDSRDSVA 273
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.321 0.136 0.412
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,364,187
Number of Sequences: 5004
Number of extensions: 224331
Number of successful extensions: 548
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 530
Number of HSP's gapped (non-prelim): 19
length of query: 1199
length of database: 2,362,478
effective HSP length: 81
effective length of query: 1118
effective length of database: 1,957,154
effective search space: 2188098172
effective search space used: 2188098172
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 59 (27.9 bits)
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