BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000843-TA|BGIBMGA000843-PA|IPR006202|Neurotransmitter- gated ion-channel ligand-binding, IPR006028|Gamma-aminobutyric acid A receptor, IPR006201|Neurotransmitter-gated ion-channel, IPR006029|Neurotransmitter-gated ion-channel transmembrane region (418 letters) Database: tribolium 317 sequences; 114,650 total letters Searching....................................................done Score E Sequences producing significant alignments: (bits) Value AF025669-1|AAB81523.1| 243|Tribolium castaneum GABA receptor su... 103 2e-24 S78567-1|AAB34900.1| 141|Tribolium castaneum GABA receptor subu... 97 2e-22 EF127808-1|ABL67945.1| 311|Tribolium castaneum nicotinic acetyl... 46 5e-07 EF127807-1|ABL67944.1| 311|Tribolium castaneum nicotinic acetyl... 44 2e-06 EF127806-1|ABL67943.1| 311|Tribolium castaneum nicotinic acetyl... 44 3e-06 EF127810-1|ABL67947.1| 311|Tribolium castaneum nicotinic acetyl... 43 5e-06 EF127809-1|ABL67946.1| 311|Tribolium castaneum nicotinic acetyl... 43 5e-06 AJ850294-1|CAH64514.1| 539|Tribolium castaneum putative esteras... 25 1.0 AJ850293-1|CAH64513.1| 539|Tribolium castaneum putative esteras... 25 1.0 AJ850292-1|CAH64512.1| 539|Tribolium castaneum putative esteras... 25 1.0 AM292372-1|CAL23184.2| 771|Tribolium castaneum gustatory recept... 23 5.4 >AF025669-1|AAB81523.1| 243|Tribolium castaneum GABA receptor subunit protein. Length = 243 Score = 103 bits (248), Expect = 2e-24 Identities = 60/189 (31%), Positives = 100/189 (52%), Gaps = 10/189 (5%) Query: 4 PARKGQPVIVEFSIYVVDVNSINVEDMDFRVDMFIRQTWSESRLQLPEDIFEEGDDHVTL 63 P G PV V ++YV+ ++S++ MDF +D + RQ W++ RL + G + +++ Sbjct: 58 PNYGGPPVEVGVTMYVLSISSLSEVKMDFTLDFYFRQFWTDPRLAYGK---RPGVETLSV 114 Query: 64 PPEFFDNLWHPDPYFLNSKVTEIAELTHKFSSVTLYRNQTVRYSARMHAIIACQMEFQLY 123 EF N+W PD +F+N K + T + ++ + ++ S R+ +C M Q Sbjct: 115 GSEFIKNIWVPDTFFVNEKQSYFHIATTSNEFIRVHHSGSITRSIRLTITASCPMNLQYL 174 Query: 124 PMDIQSCPIYIESFSYSNQKVRFRWSEA--GVTINPELKLLQYHI-GQPLRLEETNGYMI 180 PMD Q C I IESF Y+ + +R++W+E V ++ E+ L Q+ + G R E + Sbjct: 175 PMDRQLCHIEIESFGYTMRDIRYKWNEGPNSVGVSSEVSLPQFKVLGHRRRAVEIS---- 230 Query: 181 DKDGNYSRL 189 GNYSRL Sbjct: 231 LTTGNYSRL 239 >S78567-1|AAB34900.1| 141|Tribolium castaneum GABA receptor subunit protein. Length = 141 Score = 97.1 bits (231), Expect = 2e-22 Identities = 49/145 (33%), Positives = 81/145 (55%), Gaps = 7/145 (4%) Query: 132 IYIESFSYSNQKVRFRWSEA--GVTINPELKLLQYHI-GQPLRLEETNGYMIDKDGNYSR 188 I IESF Y+ + +R++W+E V ++ E+ L Q+ + G R E + GNYSR Sbjct: 1 IEIESFGYTMRDIRYKWNEGPNSVGVSNEVSLPQFKVLGHRQRAMEISL----TTGNYSR 56 Query: 189 LVVYFRFERQIGHHLIQTFAPSSLVVMLSWFSFWLDLDAIPGRXXXXXXXXXXXXXXFTG 248 L +F R +G++LIQ + PS L+V++SW SFWL+ +A P R + Sbjct: 57 LACEIQFVRSMGYYLIQIYIPSGLIVIISWVSFWLNRNATPARVALGVTTVLTMTTLMSS 116 Query: 249 LRADIPPVAYVKALDLWMAGCMMFV 273 A +P ++YVK++D+++ C + V Sbjct: 117 TNAALPKISYVKSIDVYLGTCFVMV 141 >EF127808-1|ABL67945.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 3 protein. Length = 311 Score = 46.0 bits (104), Expect = 5e-07 Identities = 61/285 (21%), Positives = 117/285 (41%), Gaps = 13/285 (4%) Query: 2 RPPARKGQPVIVEFSIYVVDVNSINVEDMDFRVDMFIRQTWSESRLQLPEDIFEEGDDHV 61 RP A + +P+ V+F + + + ++ ++ + ++ W++ L+ E + G + Sbjct: 20 RPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLKWNESEYG-GVKDL 78 Query: 62 TLPPEFFDNLWHPDPYFLNSKVTEIAELTHKFSSVTLYRNQTVRYSARMHAIIACQMEFQ 121 + P + LW PD NS E + T + ++V + N + Y C+M+ Sbjct: 79 RITP---NKLWKPDVLMYNS-ADEGFDGTFQ-TNVVVKHNGSCLYVPPGIFKSTCKMDIT 133 Query: 122 LYPMDIQSCPIYIESFSY-SNQKVRFRWSEAGVTINPELKLLQ-YHIGQPLRLEETNGYM 179 +P D Q C + S++Y NQ SE+G ++ + + Y IG P + + T Y Sbjct: 134 WFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIGMPGK-KNTIVYA 192 Query: 180 IDKDGNYSRLVVYFRFERQIGHHLIQTFAPSSLVVMLSWFSFWLDLDAIPGRXXXXXXXX 239 + Y + + R+ ++ P L+ ++ F L D+ G Sbjct: 193 CCPE-PYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDS--GEKLTLGVTI 249 Query: 240 XXXXXXFTGLRAD-IPPVAYVKALDLWMAGCMMFVFAALGEFVVV 283 F L A+ +P V+ L C+MF+ A+ VV Sbjct: 250 LLSLTVFLNLVAETLPQVSDAIPLLGTYFNCIMFMVASSVVLTVV 294 >EF127807-1|ABL67944.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 2 protein. Length = 311 Score = 44.4 bits (100), Expect = 2e-06 Identities = 60/285 (21%), Positives = 117/285 (41%), Gaps = 13/285 (4%) Query: 2 RPPARKGQPVIVEFSIYVVDVNSINVEDMDFRVDMFIRQTWSESRLQLPEDIFEEGDDHV 61 RP A + +P+ V+F + + + ++ ++ + ++ W++ L+ E + G + Sbjct: 20 RPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLKWNESEY-GGVKDL 78 Query: 62 TLPPEFFDNLWHPDPYFLNSKVTEIAELTHKFSSVTLYRNQTVRYSARMHAIIACQMEFQ 121 + P + LW PD NS E + T + ++V + N + Y C+++ Sbjct: 79 RITP---NKLWKPDVLMYNS-ADEGFDGTFQ-TNVVVKHNGSCLYVPPGIFKSTCKIDIT 133 Query: 122 LYPMDIQSCPIYIESFSY-SNQKVRFRWSEAGVTINPELKLLQ-YHIGQPLRLEETNGYM 179 +P D Q C + S++Y NQ SE+G ++ + + Y IG P + + T Y Sbjct: 134 WFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIGMPGK-KNTIVYA 192 Query: 180 IDKDGNYSRLVVYFRFERQIGHHLIQTFAPSSLVVMLSWFSFWLDLDAIPGRXXXXXXXX 239 + Y + + R+ ++ P L+ ++ F L D+ G Sbjct: 193 CCPE-PYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDS--GEKLTLGVTI 249 Query: 240 XXXXXXFTGLRAD-IPPVAYVKALDLWMAGCMMFVFAALGEFVVV 283 F L A+ +P V+ L C+MF+ A+ VV Sbjct: 250 LLSLTVFLNLVAETLPQVSDAIPLLGTYFNCIMFMVASSVVLTVV 294 >EF127806-1|ABL67943.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 1 protein. Length = 311 Score = 43.6 bits (98), Expect = 3e-06 Identities = 60/285 (21%), Positives = 115/285 (40%), Gaps = 13/285 (4%) Query: 2 RPPARKGQPVIVEFSIYVVDVNSINVEDMDFRVDMFIRQTWSESRLQLPEDIFEEGDDHV 61 RP A + +P+ V+F + + + ++ ++ + ++ W++ L+ E + G + Sbjct: 20 RPVANESEPLEVKFGLTLQQIIDVDEKNQILTTNAWLNLEWNDYNLKWNESEYG-GVKDL 78 Query: 62 TLPPEFFDNLWHPDPYFLNSKVTEIAELTHKFSSVTLYRNQTVRYSARMHAIIACQMEFQ 121 + P + LW PD NS E + T + ++V + + Y C+M+ Sbjct: 79 RITP---NKLWKPDVLMYNS-ADEGFDGTFQ-TNVVVKHGGSCLYVPPGIFKSTCKMDIT 133 Query: 122 LYPMDIQSCPIYIESFSY-SNQKVRFRWSEAGVTINPELKLLQ-YHIGQPLRLEETNGYM 179 +P D Q C + S++Y NQ SE+G ++ + + Y IG P + + T Y Sbjct: 134 WFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIGMPGK-KNTIVYA 192 Query: 180 IDKDGNYSRLVVYFRFERQIGHHLIQTFAPSSLVVMLSWFSFWLDLDAIPGRXXXXXXXX 239 + Y + + R+ ++ P L+ ++ F L D+ G Sbjct: 193 CCPE-PYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDS--GEKLTLGVTI 249 Query: 240 XXXXXXFTGLRAD-IPPVAYVKALDLWMAGCMMFVFAALGEFVVV 283 F L A+ IP + L C+MF+ A+ VV Sbjct: 250 LLSLTVFLNLVAEKIPTTSDAVPLIGTYFNCIMFMVASSVVLTVV 294 >EF127810-1|ABL67947.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 5 protein. Length = 311 Score = 42.7 bits (96), Expect = 5e-06 Identities = 60/285 (21%), Positives = 117/285 (41%), Gaps = 13/285 (4%) Query: 2 RPPARKGQPVIVEFSIYVVDVNSINVEDMDFRVDMFIRQTWSESRLQLPEDIFEEGDDHV 61 RP A + +P+ V+F + + + ++ ++ ++++ W++ L+ E + G + Sbjct: 20 RPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNIWLSLEWNDYNLKWNESEYG-GVKDL 78 Query: 62 TLPPEFFDNLWHPDPYFLNSKVTEIAELTHKFSSVTLYRNQTVRYSARMHAIIACQMEFQ 121 + P + LW PD NS E + T + ++V + + Y C+M+ Sbjct: 79 RITP---NKLWKPDVLMYNS-ADEGFDGTFQ-TNVVVKHGGSCLYVPPGIFKSTCKMDIT 133 Query: 122 LYPMDIQSCPIYIESFSY-SNQKVRFRWSEAGVTINPELKLLQ-YHIGQPLRLEETNGYM 179 +P D Q C + S++Y NQ SE+G ++ + + Y IG P + + T Y Sbjct: 134 WFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIGMPGK-KNTIVYA 192 Query: 180 IDKDGNYSRLVVYFRFERQIGHHLIQTFAPSSLVVMLSWFSFWLDLDAIPGRXXXXXXXX 239 + Y + + R+ ++ P L+ ++ F L D+ G Sbjct: 193 CCPE-PYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDS--GEKLTLGVTI 249 Query: 240 XXXXXXFTGLRAD-IPPVAYVKALDLWMAGCMMFVFAALGEFVVV 283 F L A+ +P V+ L C+MF+ A+ VV Sbjct: 250 LLSLTVFLNLVAETLPQVSDAIPLLGTYFNCIMFMVASSVVLTVV 294 >EF127809-1|ABL67946.1| 311|Tribolium castaneum nicotinic acetylcholine receptor subunitalpha 6 transcript variant 4 protein. Length = 311 Score = 42.7 bits (96), Expect = 5e-06 Identities = 60/285 (21%), Positives = 116/285 (40%), Gaps = 13/285 (4%) Query: 2 RPPARKGQPVIVEFSIYVVDVNSINVEDMDFRVDMFIRQTWSESRLQLPEDIFEEGDDHV 61 RP A + +P+ V+F + + + ++ ++ ++++ W++ L+ E + G + Sbjct: 20 RPVANESEPLEVKFGLTLQQIIDVDEKNQLLITNIWLSLEWNDYNLKWNESEYG-GVKDL 78 Query: 62 TLPPEFFDNLWHPDPYFLNSKVTEIAELTHKFSSVTLYRNQTVRYSARMHAIIACQMEFQ 121 + P + LW PD NS E + T + ++V + + Y C+M+ Sbjct: 79 RITP---NKLWKPDVLMYNS-ADEGFDGTFQ-TNVVVKHGGSCLYVPPGIFKSTCKMDIT 133 Query: 122 LYPMDIQSCPIYIESFSY-SNQKVRFRWSEAGVTINPELKLLQ-YHIGQPLRLEETNGYM 179 +P D Q C + S++Y NQ SE+G ++ + + Y IG P + + T Y Sbjct: 134 WFPFDDQHCDMKFGSWTYDGNQLDLVLNSESGGDLSDFITNGEWYLIGMPGK-KNTIVYA 192 Query: 180 IDKDGNYSRLVVYFRFERQIGHHLIQTFAPSSLVVMLSWFSFWLDLDAIPGRXXXXXXXX 239 + Y + + R+ ++ P L+ ++ F L D+ G Sbjct: 193 CCPE-PYVDVTFTIKIRRRTLYYFFNLIVPCVLISSMALLGFTLPPDS--GEKLTLGVTI 249 Query: 240 XXXXXXFTGLRAD-IPPVAYVKALDLWMAGCMMFVFAALGEFVVV 283 F L A+ IP + L C+MF+ A+ VV Sbjct: 250 LLSLTVFLNLVAEKIPTTSDAVPLIGTYFNCIMFMVASSVVLTVV 294 >AJ850294-1|CAH64514.1| 539|Tribolium castaneum putative esterase protein. Length = 539 Score = 25.0 bits (52), Expect = 1.0 Identities = 11/36 (30%), Positives = 19/36 (52%) Query: 69 DNLWHPDPYFLNSKVTEIAELTHKFSSVTLYRNQTV 104 + L+H D + N +T + + +K+ LY N TV Sbjct: 323 EGLYHLDEVYNNQLLTRLDDNWNKYIPAILYYNYTV 358 >AJ850293-1|CAH64513.1| 539|Tribolium castaneum putative esterase protein. Length = 539 Score = 25.0 bits (52), Expect = 1.0 Identities = 11/36 (30%), Positives = 19/36 (52%) Query: 69 DNLWHPDPYFLNSKVTEIAELTHKFSSVTLYRNQTV 104 + L+H D + N +T + + +K+ LY N TV Sbjct: 323 EGLYHLDEVYNNQLLTRLDDNWNKYIPAILYYNYTV 358 >AJ850292-1|CAH64512.1| 539|Tribolium castaneum putative esterase protein. Length = 539 Score = 25.0 bits (52), Expect = 1.0 Identities = 11/36 (30%), Positives = 19/36 (52%) Query: 69 DNLWHPDPYFLNSKVTEIAELTHKFSSVTLYRNQTV 104 + L+H D + N +T + + +K+ LY N TV Sbjct: 323 EGLYHLDEVYNNQLLTRLDDNWNKYIPAILYYNYTV 358 >AM292372-1|CAL23184.2| 771|Tribolium castaneum gustatory receptor candidate 51 protein. Length = 771 Score = 22.6 bits (46), Expect = 5.4 Identities = 9/26 (34%), Positives = 15/26 (57%) Query: 203 LIQTFAPSSLVVMLSWFSFWLDLDAI 228 L + +A +VV + W +WL DA+ Sbjct: 43 LAKCYACLLMVVKILWVIYWLQDDAL 68 Database: tribolium Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 114,650 Number of sequences in database: 317 Lambda K H 0.325 0.139 0.435 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 92,427 Number of Sequences: 317 Number of extensions: 3878 Number of successful extensions: 25 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 7 Number of HSP's gapped (non-prelim): 11 length of query: 418 length of database: 114,650 effective HSP length: 58 effective length of query: 360 effective length of database: 96,264 effective search space: 34655040 effective search space used: 34655040 T: 11 A: 40 X1: 15 ( 7.0 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.6 bits) S2: 44 (21.8 bits)
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