BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000837-TA|BGIBMGA000837-PA|IPR002018|Carboxylesterase, type B (545 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone este... 184 1e-48 AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein. 184 1e-48 AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 160 2e-41 AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 133 4e-33 AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein. 106 5e-25 AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein. 50 4e-08 AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 26 0.69 DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride... 24 3.7 DQ288391-1|ABC41341.1| 630|Apis mellifera vasa protein protein. 23 8.6 >AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone esterase protein. Length = 567 Score = 184 bits (449), Expect = 1e-48 Identities = 155/543 (28%), Positives = 247/543 (45%), Gaps = 41/543 (7%) Query: 26 PLVDTKVGLIRGLTSEDGS---YSMFMGIPYANVNYSNPFGDAIPHT--KFQDTFEAYDD 80 P V T +G I+G G+ Y + GIPYA + F P + A Sbjct: 23 PRVKTPLGAIKGYYKISGNGKQYEAYEGIPYA-LPPVGKFRFKAPQKIPAWIGELSATKF 81 Query: 81 GAICPQLYMF----NETVLGNLDCLHLNIYVPSTATSKKPLPIMVWIHGGSFRRGFAGRY 136 G C Q + + G DCL+LN+YVP+ T + LP++ WIHGG+F+ G +G Sbjct: 82 GFPCLQYTQLPVNPRDKIEGAEDCLYLNVYVPADRTPSQSLPVIFWIHGGAFQFG-SGIP 140 Query: 137 MYDPKFLVKHNVISVNINYRLGPYGFMCLDHPSVPGNQGLKDQTLALRWIKDNIEAFGGD 196 M K+L+ +VI V INYRLG GF+ + VPGN GLKDQ++ALRW+ +NIE FGG+ Sbjct: 141 M-GAKYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGGN 199 Query: 197 PNEITVFGESAGGHSVDFHLLSN-NEVLFHRAIIQSGSTEAGTVLIEPNIEAPIKLADYL 255 P IT+ G SAGG SV +H LS + LF I SG+ E ++E ++ ++ Sbjct: 200 PKRITLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSLEKAKQVGAFM 259 Query: 256 GYQTSEIEEALNFLITADTDLVIGAALDLN-------IMFKPCTENEFDGVNG--FITKH 306 G T ++E + L ++ + F P TE + N FI + Sbjct: 260 GCPTRNVKEMIRCLRYRPARAIVETLANFMRFYYNPFTPFGPVTEKVNNDSNSLPFIDRT 319 Query: 307 WVN-TEVPKVRKMPVLIGFNENELL--INHVNKGNEHFTTLD-----IFRTYLEQTFTFT 358 + V+ +P + G E L + E LD I +L+ +T Sbjct: 320 PIEIINSGDVQDVPWVTGVTSEEGLYPVAEFIAKPEALKLLDENWDLIAPYFLDYNYTIP 379 Query: 359 NEELDDMSQLVKNFYLGDNPISEDEKWNVIYYDSDFTYIHPIQRTINKFLKNGVGDIFYY 418 E+ ++++L++N+Y N I E ++I SD +I ++ K +++Y Sbjct: 380 KEKHVEVARLIRNYYFESNKIDETTLKHLIDVASDRFFITDGEKAARMQAKVNRQPVWFY 439 Query: 419 MFAYDGGRNAFKIANGI-NATGVSHADELGYLFNMNYVTGEITPEDKTMIDRMTTMWTNF 477 + Y G + +I +G N GV HAD+ + + ++ T D M + W +F Sbjct: 440 YYTYKGAHSISEIMSGTSNKYGVCHADDAYMVVDTPFLASTTTTNDIKMQKVLIDFWVSF 499 Query: 478 AKYGNPTPETSELLPIKWEPV--TKQKLNYLKI--DAELSLGNRPLNHRMAFWDMFYKMN 533 G P + ++W + ++ L+YL I ++ + + R FW+ N Sbjct: 500 VNNGVPNVNS-----VQWPRLNPNEKSLHYLHIAGPGKIQMDSSTNFGREDFWNSI-NFN 553 Query: 534 EEK 536 E K Sbjct: 554 ENK 556 >AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein. Length = 567 Score = 184 bits (449), Expect = 1e-48 Identities = 155/543 (28%), Positives = 247/543 (45%), Gaps = 41/543 (7%) Query: 26 PLVDTKVGLIRGLTSEDGS---YSMFMGIPYANVNYSNPFGDAIPHT--KFQDTFEAYDD 80 P V T +G I+G G+ Y + GIPYA + F P + A Sbjct: 23 PRVKTPLGAIKGYYKISGNGKQYEAYEGIPYA-LPPVGKFRFKAPQKIPAWIGELSATKF 81 Query: 81 GAICPQLYMF----NETVLGNLDCLHLNIYVPSTATSKKPLPIMVWIHGGSFRRGFAGRY 136 G C Q + + G DCL+LN+YVP+ T + LP++ WIHGG+F+ G +G Sbjct: 82 GFPCLQYTQLPVNPRDKIEGAEDCLYLNVYVPADRTPSQSLPVIFWIHGGAFQFG-SGIP 140 Query: 137 MYDPKFLVKHNVISVNINYRLGPYGFMCLDHPSVPGNQGLKDQTLALRWIKDNIEAFGGD 196 M K+L+ +VI V INYRLG GF+ + VPGN GLKDQ++ALRW+ +NIE FGG+ Sbjct: 141 M-GAKYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGGN 199 Query: 197 PNEITVFGESAGGHSVDFHLLSN-NEVLFHRAIIQSGSTEAGTVLIEPNIEAPIKLADYL 255 P IT+ G SAGG SV +H LS + LF I SG+ E ++E ++ ++ Sbjct: 200 PKRITLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSLEKAKQVGAFM 259 Query: 256 GYQTSEIEEALNFLITADTDLVIGAALDLN-------IMFKPCTENEFDGVNG--FITKH 306 G T ++E + L ++ + F P TE + N FI + Sbjct: 260 GCPTRNVKEMIRCLRYRPARAIVETLANFMRFYYNPFTPFGPVTEKVNNDSNSLPFIDRT 319 Query: 307 WVN-TEVPKVRKMPVLIGFNENELL--INHVNKGNEHFTTLD-----IFRTYLEQTFTFT 358 + V+ +P + G E L + E LD I +L+ +T Sbjct: 320 PIEIINSGDVQDVPWVTGVTSEEGLYPVAEFIAKPEALKLLDENWDLIAPYFLDYNYTIP 379 Query: 359 NEELDDMSQLVKNFYLGDNPISEDEKWNVIYYDSDFTYIHPIQRTINKFLKNGVGDIFYY 418 E+ ++++L++N+Y N I E ++I SD +I ++ K +++Y Sbjct: 380 KEKHVEVARLIRNYYFESNKIDETTLKHLIDVASDRFFITDGEKAARMQAKVNRQPVWFY 439 Query: 419 MFAYDGGRNAFKIANGI-NATGVSHADELGYLFNMNYVTGEITPEDKTMIDRMTTMWTNF 477 + Y G + +I +G N GV HAD+ + + ++ T D M + W +F Sbjct: 440 YYTYKGAHSISEIMSGTSNKYGVCHADDAYMVVDTPFLASTTTTNDIKMQKVLIDFWVSF 499 Query: 478 AKYGNPTPETSELLPIKWEPV--TKQKLNYLKI--DAELSLGNRPLNHRMAFWDMFYKMN 533 G P + ++W + ++ L+YL I ++ + + R FW+ N Sbjct: 500 VNNGVPNVNS-----VQWPRLNPNEKSLHYLHIAGPGKIQMDSSTNFGREDFWNSI-NFN 553 Query: 534 EEK 536 E K Sbjct: 554 ENK 556 >AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase protein. Length = 628 Score = 160 bits (389), Expect = 2e-41 Identities = 169/562 (30%), Positives = 243/562 (43%), Gaps = 74/562 (13%) Query: 25 DPLV-DTKVGLIRGL--TSEDGSYSMFMGIPYAN-----VNYSNPF----------GDAI 66 DPLV +T GL+RG T D +F GIP+A + + P + Sbjct: 36 DPLVVETTSGLVRGFPRTVLDKEVHVFYGIPFAKPPIGPLRFRKPLPIEPWHGVLNATVL 95 Query: 67 PHTKFQDTFEAYDDGAICPQLYMFNETVLGNLDCLHLNIYVP-----------STATSKK 115 P++ +Q+ +E Y G P M+N + DCL+LNI+VP S + Sbjct: 96 PNSCYQERYE-YFPGF--PGEEMWNPNTNISEDCLYLNIWVPQKYRLRHKGDGSPGGNGG 152 Query: 116 P----LPIMVWIHGGSFRRGFAGRYMYDPKFLVK-HNVISVNINYRLGPYGFMCLDH--- 167 P LP++VWI+GG F G A +Y+ + NVI ++ YR+G +GF+ L+ Sbjct: 153 PRNGLLPLLVWIYGGGFMSGTATLDVYNADIMAATSNVIIASMQYRVGAFGFLYLNKHFT 212 Query: 168 --PSVPGNQGLKDQTLALRWIKDNIEAFGGDPNEITVFGESAGGHSVDFHLLSN-NEVLF 224 PGN GL DQ LALRW++DN EAFGGDP IT+FGESAGG SV HL+S L Sbjct: 213 NSEEAPGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSVSLHLISPVTRGLV 272 Query: 225 HRAIIQSGSTEAGTVLI--EPNIEAPIKLADYLGYQTSEIEE------ALNFLITADTDL 276 R I+QSG+ A + E E L D G ++ + E A + A T Sbjct: 273 RRGILQSGTLNAPWSYMSGEKANEVATILVDDCGCNSTMLNENPARVMACMRSVDAKTIS 332 Query: 277 VIGAALDLNIMFKPCTENEFDGVNGFITKHWVN-TEVPKVRKMPVLIGFNENELLINHVN 335 V I+ P DG+ F+ KH ++ + +LIG NENE + Sbjct: 333 VQQWNSYWGILGFPSAPT-IDGI--FLPKHPLDLLREADFKDTEILIGNNENEGTYFILY 389 Query: 336 KGNEHFTTLDIFRTYLEQTFTFTNEELDDMSQLVKNFYLGDNPISEDEKWNVIYYD---- 391 N+ F E+ N +MSQ+ + E+ IY Sbjct: 390 DFNDIFEKDQASFLERERFLGIINNIFKNMSQIEREAITFQYTDWEEVYNGYIYQKMVAD 449 Query: 392 --SDFTYIHPIQRTINKFLKNGVGDIFYYMFAYDGGRNAFKIANGINATGVSHADELGYL 449 D+ +I P F G+ ++YY F N + GV H DE+ Y+ Sbjct: 450 VVGDYFFICPSIHFAQLFADRGM-KVYYYFFTQRTSTNLWG-----EWMGVLHGDEVEYV 503 Query: 450 FNMNYVTG-EITPEDKTMIDRMTTMWTNFAKYGNPTPETSELLPIKWEPVTKQKLNYLKI 508 F + + +++ + RM ++ FA G PT E SE W ++ + Y Sbjct: 504 FGHPLNKSLKYSDKERDLSLRMILYFSEFAYLGKPTKEDSE-----WPSYSRDEPKYFIF 558 Query: 509 DAE-LSLGNRPLNHRMAFWDMF 529 DAE LG P AFW+ F Sbjct: 559 DAEKTGLGKGPRTTYCAFWNEF 580 >AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase protein. Length = 492 Score = 133 bits (321), Expect = 4e-33 Identities = 97/252 (38%), Positives = 132/252 (52%), Gaps = 43/252 (17%) Query: 25 DPLV-DTKVGLIRGL--TSEDGSYSMFMGIPYAN-----VNYSNPF----------GDAI 66 DPLV +T GL+RG T D +F GIP+A + + P + Sbjct: 36 DPLVVETTSGLVRGFPRTVLDKEVHVFYGIPFAKPPIGPLRFRKPLPIEPWHGVLNATVL 95 Query: 67 PHTKFQDTFEAYDDGAICPQLYMFNETVLGNLDCLHLNIYVP-----------STATSKK 115 P++ +Q+ +E Y G P M+N + DCL+LNI+VP S + Sbjct: 96 PNSCYQERYE-YFPGF--PGEEMWNPNTNISEDCLYLNIWVPQKYRLRHKGDGSPGGNGG 152 Query: 116 P----LPIMVWIHGGSFRRGFAGRYMYDPKFLVK-HNVISVNINYRLGPYGFMCLDH--- 167 P LP++VWI+GG F G A +Y+ + NVI ++ YR+G +GF+ L+ Sbjct: 153 PRNGLLPLLVWIYGGGFMSGTATLDVYNADIMAATSNVIIASMQYRVGAFGFLYLNKHFT 212 Query: 168 --PSVPGNQGLKDQTLALRWIKDNIEAFGGDPNEITVFGESAGGHSVDFHLLSN-NEVLF 224 PGN GL DQ LALRW++DN EAFGGDP IT+FGESAGG SV HL+S L Sbjct: 213 NSEEAPGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSVSLHLISPVTRGLV 272 Query: 225 HRAIIQSGSTEA 236 R I+QSG+ A Sbjct: 273 RRGILQSGTLNA 284 >AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein. Length = 169 Score = 106 bits (254), Expect = 5e-25 Identities = 56/130 (43%), Positives = 79/130 (60%), Gaps = 1/130 (0%) Query: 141 KFLVKHNVISVNINYRLGPYGFMCLDHPSVPGNQGLKDQTLALRWIKDNIEAFGGDPNEI 200 K+L+ +VI V INYRLG GF+ + VPGN GLKDQ++ALRW+ +NIE FGG+P I Sbjct: 15 KYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGGNPKRI 74 Query: 201 TVFGESAGGHSVDFHLLSN-NEVLFHRAIIQSGSTEAGTVLIEPNIEAPIKLADYLGYQT 259 T+ G SAGG SV +H LS + LF I SG+ E ++E ++ ++G T Sbjct: 75 TLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSLEKAKQVGAFMGCPT 134 Query: 260 SEIEEALNFL 269 ++E + L Sbjct: 135 RNVKEMIRCL 144 >AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein. Length = 62 Score = 50.4 bits (115), Expect = 4e-08 Identities = 23/63 (36%), Positives = 39/63 (61%), Gaps = 3/63 (4%) Query: 99 DCLHLNIYVPSTATSKKPLPIMVWIHGGSFRRGFAGRYMYDPKFLVKHNVISVNINYRLG 158 DCL+L++Y S SK P+M ++H G+F G + + P +L+ +V+ V+ NYR+G Sbjct: 3 DCLYLDVYTNSLDQSK---PVMFYVHEGAFISGTSSFHEMRPDYLLPKDVVVVSSNYRVG 59 Query: 159 PYG 161 +G Sbjct: 60 AFG 62 >AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase protein. Length = 580 Score = 26.2 bits (55), Expect = 0.69 Identities = 17/44 (38%), Positives = 22/44 (50%), Gaps = 3/44 (6%) Query: 505 YLKIDAELSLGNR--PLNHRMAFWDMFYKMNEE-KQAYLENSST 545 Y+ DA + G R P N FW ++ NEE KQ YL +T Sbjct: 146 YVWRDARIVNGTRQPPNNWLSVFWGSAWQWNEERKQYYLHQFAT 189 >DQ667190-1|ABG75742.1| 509|Apis mellifera pH-sensitive chloride channel variant 1 protein. Length = 509 Score = 23.8 bits (49), Expect = 3.7 Identities = 10/32 (31%), Positives = 17/32 (53%) Query: 325 NENELLINHVNKGNEHFTTLDIFRTYLEQTFT 356 + N+ + N+ N H+T+ RT+L T T Sbjct: 107 SSNDPKNQYKNQNNNHYTSHQHLRTHLRGTLT 138 >DQ288391-1|ABC41341.1| 630|Apis mellifera vasa protein protein. Length = 630 Score = 22.6 bits (46), Expect = 8.6 Identities = 8/29 (27%), Positives = 16/29 (55%) Query: 454 YVTGEITPEDKTMIDRMTTMWTNFAKYGN 482 Y+ E+ ++K++ + + NF KY N Sbjct: 153 YIPPELPNDEKSLFENGVEIGINFDKYDN 181 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.320 0.139 0.421 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 170,397 Number of Sequences: 429 Number of extensions: 8282 Number of successful extensions: 37 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 16 Number of HSP's gapped (non-prelim): 13 length of query: 545 length of database: 140,377 effective HSP length: 61 effective length of query: 484 effective length of database: 114,208 effective search space: 55276672 effective search space used: 55276672 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 46 (22.6 bits)
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