Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000825-TA|BGIBMGA000825-PA|IPR004299|Membrane bound
O-acyl transferase, MBOAT
         (392 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC16A3.10 |||membrane bound O-acyltransferase, MBOAT |Schizosa...   142   8e-35
SPCC1919.12c |||aminopeptidase |Schizosaccharomyces pombe|chr 3|...    27   4.6  
SPAC1B2.02c |ugo1||mitochondrial fusion and transport protein Ug...    27   4.6  
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ...    26   8.0  

>SPBC16A3.10 |||membrane bound O-acyltransferase, MBOAT
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 509

 Score =  142 bits (344), Expect = 8e-35
 Identities = 90/297 (30%), Positives = 153/297 (51%), Gaps = 20/297 (6%)

Query: 15  ITFVFNMGYLLIGYIVT---ESEDYDITWTMPHCVLTLKLMALSFDLWDGKKMIKGETLS 71
           I F+  +G+    +++     SE+ DIT +    VL +KL A ++ ++DG+  +    LS
Sbjct: 96  IIFIVILGHTFSSHVIRYIYPSENTDITASQ--MVLCMKLTAFAWSVYDGR--LPSSELS 151

Query: 72  ENNKKTALLRAPSFVELIGFVYFPACFLVGPIFSFRRYIDFV-MDKF-----PVEKEAKV 125
              K  AL + P+ +  +G+V+F    LVGP F +  Y  F+ +  F     P EK+   
Sbjct: 152 SYQKDRALRKIPNILYFLGYVFFFPSLLVGPAFDYVDYERFITLSMFKPLADPYEKQITP 211

Query: 126 YEKQ-ALRRLLQGLGYLIAFQVGVYVFNVKYMLSDDFWETSIFYRHFYCGMWAHFALYKY 184
           +  + AL R  +GL +LI F  G  ++ +K++L+  F  + I  ++ Y  + A  A  KY
Sbjct: 212 HSLEPALGRCWRGLLWLILFITGSSIYPLKFLLTPKFASSPILLKYGYVCITAFVARMKY 271

Query: 185 ISCWLLTEASCIRFGMSYNGVETTPEGKTISKWDGCNNIKLLRFEGATKFQHYIDSFNCN 244
              W L++ +CI  G+ YNG++++       +WD   NI  ++FE A   +  ++++N N
Sbjct: 272 YGAWELSDGACILSGIGYNGLDSSKH----PRWDRVKNIDPIKFEFADNIKCALEAWNMN 327

Query: 245 TNHFAAEYIYKRLKFLGNRN--LSQFFTLLFLALWHGTRSGYYMTFFNEFLIMLMEK 299
           TN +   Y+Y R+   G R    S   T    A+WHG  +GYY+TF +   I  + K
Sbjct: 328 TNKWLRNYVYLRVAKKGKRPGFKSTLSTFTVSAMWHGVSAGYYLTFVSAAFIQTVAK 384


>SPCC1919.12c |||aminopeptidase |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 843

 Score = 27.1 bits (57), Expect = 4.6
 Identities = 22/77 (28%), Positives = 33/77 (42%), Gaps = 4/77 (5%)

Query: 194 SCIRFGMSYNGVETTPEGKTISKWDGCNNIKLLRFEGATKFQHYIDS----FNCNTNHFA 249
           +C  FG +   +    + K I++  G N +K L F+ A     YI S    +N   N   
Sbjct: 22  TCSIFGFTVLLILYLLDWKRIAQVPGPNLLKDLEFQRAWNDLEYISSLPHPYNSKQNEHV 81

Query: 250 AEYIYKRLKFLGNRNLS 266
             YI K ++ L   N S
Sbjct: 82  RSYILKSMRELEATNQS 98


>SPAC1B2.02c |ugo1||mitochondrial fusion and transport protein
           Ugo1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 421

 Score = 27.1 bits (57), Expect = 4.6
 Identities = 12/24 (50%), Positives = 17/24 (70%)

Query: 300 ELEPIISKTELYNKLWSHNVTKYM 323
           E +P ISK+E++  L S  VTKY+
Sbjct: 26  ETQPTISKSEIFETLRSILVTKYL 49


>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 547

 Score = 26.2 bits (55), Expect = 8.0
 Identities = 10/24 (41%), Positives = 14/24 (58%)

Query: 220 CNNIKLLRFEGATKFQHYIDSFNC 243
           C NI   R+  A   QHY+D+ +C
Sbjct: 277 CGNIGCGRYHDAHAKQHYVDTAHC 300


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.329    0.142    0.460 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,877,017
Number of Sequences: 5004
Number of extensions: 84088
Number of successful extensions: 233
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 226
Number of HSP's gapped (non-prelim): 5
length of query: 392
length of database: 2,362,478
effective HSP length: 74
effective length of query: 318
effective length of database: 1,992,182
effective search space: 633513876
effective search space used: 633513876
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.8 bits)
S2: 55 (26.2 bits)