Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000825-TA|BGIBMGA000825-PA|IPR004299|Membrane bound
O-acyl transferase, MBOAT
         (392 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g63050.1 68414.m07122 membrane bound O-acyl transferase (MBOA...   101   1e-21
At1g12640.1 68414.m01468 membrane bound O-acyl transferase (MBOA...   100   1e-21
At4g12880.1 68417.m02016 plastocyanin-like domain-containing pro...    31   1.8  
At3g14150.1 68416.m01789 (S)-2-hydroxy-acid oxidase, peroxisomal...    29   5.4  
At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 fam...    29   5.4  
At3g45940.1 68416.m04971 alpha-xylosidase, putative strong simil...    29   7.1  

>At1g63050.1 68414.m07122 membrane bound O-acyl transferase (MBOAT)
           family protein low similarity to porcupine from [Xenopus
           laevis] GI:6714514, GI:6714520, GI:6714518, GI:6714516;
           contains Pfam profile PF03062: MBOAT family
          Length = 465

 Score =  101 bits (241), Expect = 1e-21
 Identities = 97/374 (25%), Positives = 167/374 (44%), Gaps = 25/374 (6%)

Query: 11  MNAVITFVFNMGYLLIGYIVTESEDY----DITWTMPHCVLTLKLMALSFDLWDGKKMIK 66
           ++  ITF     YL+  ++   S D      I  T    VLTLK+++ S +  DG  M+K
Sbjct: 86  LSGFITFFLGFAYLIGCHVFYMSGDAWKEGGIDSTGALMVLTLKVISCSINYNDG--MLK 143

Query: 67  GETLSENNKKTALLRAPSFVELIGFVYFPACFLVGPIFSFRRYIDFVMDK--FPVEKEAK 124
            E L E  KK  L++ PS +E  G+         GP+F  + Y+++  +K  + V ++ K
Sbjct: 144 EEGLREAQKKNRLIQMPSLIEYFGYCLCCGSHFAGPVFEMKDYLEWTEEKGIWAVSEKGK 203

Query: 125 VYEKQA--LRRLLQGLGYLIAFQVGVYVFNVKYMLSDDFWETSIFYRHFYCGMWAHFALY 182
                   +R + Q    +  +   V  F +       + E     R  Y  M    A +
Sbjct: 204 RPSPYGAMIRAVFQAAICMALYLYLVPQFPLTRFTEPVYQEWGFLKRFGYQYMAGFTARW 263

Query: 183 KYISCWLLTEASCIRFGMSYNGVETTPEGKTISKWDGCNNIKLLRFEGATKFQHYIDSFN 242
           KY   W ++EAS I  G+ ++G   T E +T +KWD   N+ +L  E A         +N
Sbjct: 264 KYYFIWSISEASIIISGLGFSG--WTDETQTKAKWDRAKNVDILGVELAKSAVQIPLFWN 321

Query: 243 CNTNHFAAEYIYKRLKFLGNRNLSQFFTLL----FLALWHGTRSGYYMTFFNEFLIMLME 298
              + +   Y+Y+R+   G +  + FF LL      A+WHG   GY + F    L++   
Sbjct: 322 IQVSTWLRHYVYERIVKPGKK--AGFFQLLATQTVSAVWHGLYPGYIIFFVQSALMIDGS 379

Query: 299 KELEPIISKTELYNKLWSHNVTKYMLYGLLKMYTIVFMGWSLAPFDLKAFSKWWGVYSSL 358
           K +     +  +  K+    + + +L  +  +YT+V + +S   F + +  +    + S+
Sbjct: 380 KAI--YRWQQAIPPKM---AMLRNVLVLINFLYTVVVLNYSSVGFMVLSLHETLVAFKSV 434

Query: 359 YYSGFVLFLPWAIL 372
           YY G V  +P A+L
Sbjct: 435 YYIGTV--IPIAVL 446


>At1g12640.1 68414.m01468 membrane bound O-acyl transferase (MBOAT)
           family protein low similarity to porcupine from [Xenopus
           laevis] GI:6714514, GI:6714520, GI:6714518, GI:6714516;
           contains Pfam profile PF03062: MBOAT family
          Length = 462

 Score =  100 bits (240), Expect = 1e-21
 Identities = 92/364 (25%), Positives = 156/364 (42%), Gaps = 23/364 (6%)

Query: 14  VITFVFNMGYLLIGYIVTESEDY----DITWTMPHCVLTLKLMALSFDLWDGKKMIKGET 69
           +ITF     YL+  ++   S D      I  T    VLTLK+++ S +  DG  M+K E 
Sbjct: 86  IITFFLGFAYLIGCHVFYMSGDAWKEGGIDSTGALMVLTLKVISCSMNYNDG--MLKEEG 143

Query: 70  LSENNKKTALLRAPSFVELIGFVYFPACFLVGPIFSFRRYIDFVMDK---FPVEKEAKVY 126
           L E  KK  L++ PS +E  G+         GP++  + Y+++   K      EK  K  
Sbjct: 144 LREAQKKNRLIQMPSLIEYFGYCLCCGSHFAGPVYEMKDYLEWTEGKGIWDTTEKRKKPS 203

Query: 127 EKQA-LRRLLQGLGYLIAFQVGVYVFNVKYMLSDDFWETSIFYRHFYCGMWAHFALYKYI 185
              A +R +LQ    +  +   V  + +       + E     +  Y  M    A +KY 
Sbjct: 204 PYGATIRAILQAAICMALYLYLVPQYPLTRFTEPVYQEWGFLRKFSYQYMAGFTARWKYY 263

Query: 186 SCWLLTEASCIRFGMSYNGVETTPEGKTISKWDGCNNIKLLRFEGATKFQHYIDSFNCNT 245
             W ++EAS I  G+ ++G   T +     KWD   N+ +L  E A         +N   
Sbjct: 264 FIWSISEASIIISGLGFSG--WTDDASPKPKWDRAKNVDILGVELAKSAVQIPLVWNIQV 321

Query: 246 NHFAAEYIYKRLKFLGNRNLSQFFTLL----FLALWHGTRSGYYMTFFNEFLIMLMEKEL 301
           + +   Y+Y+RL  + N   + FF LL      A+WHG   G YM FF +  +M+    +
Sbjct: 322 STWLRHYVYERL--VQNGKKAGFFQLLATQTVSAVWHGLYPG-YMMFFVQSALMIAGSRV 378

Query: 302 EPIISKTELYNKLWSHNVTKYMLYGLLKMYTIVFMGWSLAPFDLKAFSKWWGVYSSLYYS 361
                +          N+  ++ +    +YT++ + +S   F + +  +    Y S+YY 
Sbjct: 379 IYRWQQAISPKMAMLRNIMVFINF----LYTVLVLNYSAVGFMVLSLHETLTAYGSVYYI 434

Query: 362 GFVL 365
           G ++
Sbjct: 435 GTII 438


>At4g12880.1 68417.m02016 plastocyanin-like domain-containing
           protein
          Length = 141

 Score = 30.7 bits (66), Expect = 1.8
 Identities = 18/53 (33%), Positives = 27/53 (50%), Gaps = 8/53 (15%)

Query: 138 LGYLIAFQVGVYVFNVKYMLSDD-FWETSIFY------RHFYCGMWAHFALYK 183
           L +L+A  +   V   KY++ D  FW  +I Y      +HFY G W +F  Y+
Sbjct: 13  LAFLVAAPIPE-VTAKKYLVGDKKFWNPNINYTLWAQGKHFYVGDWLYFVFYR 64


>At3g14150.1 68416.m01789 (S)-2-hydroxy-acid oxidase, peroxisomal,
           putative / glycolate oxidase, putative / short chain
           alpha-hydroxy acid oxidase, putative similar to
           (S)-2-hydroxy-acid oxidase, peroxisomal (Glycolate
           oxidase, GOX) (Short chain alpha-hydroxy acid oxidase)
           [Spinacia oleracea] SWISS-PROT:P05414
          Length = 363

 Score = 29.1 bits (62), Expect = 5.4
 Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 4/50 (8%)

Query: 209 PEGKTISKWDG--CNNIKLLRFEGATKFQHYIDSFNCNTNHFAAEYIYKR 256
           PEG+T +      CN I ++ +  +  F+    S  CN   F   Y+YKR
Sbjct: 87  PEGETATAKAAAACNTIMIVSYMSSCTFEEIASS--CNAVRFLQIYVYKR 134


>At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 family
           protein similar to  PHO1 protein [Arabidopsis thaliana]
           GI:20069032; contains Pfam profiles PF03105: SPX domain,
           PF03124: EXS family
          Length = 756

 Score = 29.1 bits (62), Expect = 5.4
 Identities = 18/56 (32%), Positives = 28/56 (50%)

Query: 113 VMDKFPVEKEAKVYEKQALRRLLQGLGYLIAFQVGVYVFNVKYMLSDDFWETSIFY 168
           V+    +E + K  + QAL  LL     +  F V V  FN+ Y  S  F+ T++F+
Sbjct: 457 VLANLDMETDPKTKDYQALTELLPLFLLIAMFVVLVVPFNIFYRSSRFFFLTTLFH 512


>At3g45940.1 68416.m04971 alpha-xylosidase, putative strong
           similarity to alpha-xylosidase precursor GI:4163997 from
           [Arabidopsis thaliana]
          Length = 868

 Score = 28.7 bits (61), Expect = 7.1
 Identities = 15/52 (28%), Positives = 26/52 (50%)

Query: 221 NNIKLLRFEGATKFQHYIDSFNCNTNHFAAEYIYKRLKFLGNRNLSQFFTLL 272
           N IKL+  E  T F   + +FN NT+ + +  +Y  L+ +  +  +    LL
Sbjct: 184 NGIKLVPNEPYTLFTEDVSAFNLNTDLYGSHPVYMDLRNVSGKAYAHSVLLL 235


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.329    0.142    0.460 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,419,870
Number of Sequences: 28952
Number of extensions: 410373
Number of successful extensions: 1083
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1073
Number of HSP's gapped (non-prelim): 7
length of query: 392
length of database: 12,070,560
effective HSP length: 82
effective length of query: 310
effective length of database: 9,696,496
effective search space: 3005913760
effective search space used: 3005913760
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.8 bits)
S2: 60 (28.3 bits)