BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000825-TA|BGIBMGA000825-PA|IPR004299|Membrane bound O-acyl transferase, MBOAT (392 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g63050.1 68414.m07122 membrane bound O-acyl transferase (MBOA... 101 1e-21 At1g12640.1 68414.m01468 membrane bound O-acyl transferase (MBOA... 100 1e-21 At4g12880.1 68417.m02016 plastocyanin-like domain-containing pro... 31 1.8 At3g14150.1 68416.m01789 (S)-2-hydroxy-acid oxidase, peroxisomal... 29 5.4 At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 fam... 29 5.4 At3g45940.1 68416.m04971 alpha-xylosidase, putative strong simil... 29 7.1 >At1g63050.1 68414.m07122 membrane bound O-acyl transferase (MBOAT) family protein low similarity to porcupine from [Xenopus laevis] GI:6714514, GI:6714520, GI:6714518, GI:6714516; contains Pfam profile PF03062: MBOAT family Length = 465 Score = 101 bits (241), Expect = 1e-21 Identities = 97/374 (25%), Positives = 167/374 (44%), Gaps = 25/374 (6%) Query: 11 MNAVITFVFNMGYLLIGYIVTESEDY----DITWTMPHCVLTLKLMALSFDLWDGKKMIK 66 ++ ITF YL+ ++ S D I T VLTLK+++ S + DG M+K Sbjct: 86 LSGFITFFLGFAYLIGCHVFYMSGDAWKEGGIDSTGALMVLTLKVISCSINYNDG--MLK 143 Query: 67 GETLSENNKKTALLRAPSFVELIGFVYFPACFLVGPIFSFRRYIDFVMDK--FPVEKEAK 124 E L E KK L++ PS +E G+ GP+F + Y+++ +K + V ++ K Sbjct: 144 EEGLREAQKKNRLIQMPSLIEYFGYCLCCGSHFAGPVFEMKDYLEWTEEKGIWAVSEKGK 203 Query: 125 VYEKQA--LRRLLQGLGYLIAFQVGVYVFNVKYMLSDDFWETSIFYRHFYCGMWAHFALY 182 +R + Q + + V F + + E R Y M A + Sbjct: 204 RPSPYGAMIRAVFQAAICMALYLYLVPQFPLTRFTEPVYQEWGFLKRFGYQYMAGFTARW 263 Query: 183 KYISCWLLTEASCIRFGMSYNGVETTPEGKTISKWDGCNNIKLLRFEGATKFQHYIDSFN 242 KY W ++EAS I G+ ++G T E +T +KWD N+ +L E A +N Sbjct: 264 KYYFIWSISEASIIISGLGFSG--WTDETQTKAKWDRAKNVDILGVELAKSAVQIPLFWN 321 Query: 243 CNTNHFAAEYIYKRLKFLGNRNLSQFFTLL----FLALWHGTRSGYYMTFFNEFLIMLME 298 + + Y+Y+R+ G + + FF LL A+WHG GY + F L++ Sbjct: 322 IQVSTWLRHYVYERIVKPGKK--AGFFQLLATQTVSAVWHGLYPGYIIFFVQSALMIDGS 379 Query: 299 KELEPIISKTELYNKLWSHNVTKYMLYGLLKMYTIVFMGWSLAPFDLKAFSKWWGVYSSL 358 K + + + K+ + + +L + +YT+V + +S F + + + + S+ Sbjct: 380 KAI--YRWQQAIPPKM---AMLRNVLVLINFLYTVVVLNYSSVGFMVLSLHETLVAFKSV 434 Query: 359 YYSGFVLFLPWAIL 372 YY G V +P A+L Sbjct: 435 YYIGTV--IPIAVL 446 >At1g12640.1 68414.m01468 membrane bound O-acyl transferase (MBOAT) family protein low similarity to porcupine from [Xenopus laevis] GI:6714514, GI:6714520, GI:6714518, GI:6714516; contains Pfam profile PF03062: MBOAT family Length = 462 Score = 100 bits (240), Expect = 1e-21 Identities = 92/364 (25%), Positives = 156/364 (42%), Gaps = 23/364 (6%) Query: 14 VITFVFNMGYLLIGYIVTESEDY----DITWTMPHCVLTLKLMALSFDLWDGKKMIKGET 69 +ITF YL+ ++ S D I T VLTLK+++ S + DG M+K E Sbjct: 86 IITFFLGFAYLIGCHVFYMSGDAWKEGGIDSTGALMVLTLKVISCSMNYNDG--MLKEEG 143 Query: 70 LSENNKKTALLRAPSFVELIGFVYFPACFLVGPIFSFRRYIDFVMDK---FPVEKEAKVY 126 L E KK L++ PS +E G+ GP++ + Y+++ K EK K Sbjct: 144 LREAQKKNRLIQMPSLIEYFGYCLCCGSHFAGPVYEMKDYLEWTEGKGIWDTTEKRKKPS 203 Query: 127 EKQA-LRRLLQGLGYLIAFQVGVYVFNVKYMLSDDFWETSIFYRHFYCGMWAHFALYKYI 185 A +R +LQ + + V + + + E + Y M A +KY Sbjct: 204 PYGATIRAILQAAICMALYLYLVPQYPLTRFTEPVYQEWGFLRKFSYQYMAGFTARWKYY 263 Query: 186 SCWLLTEASCIRFGMSYNGVETTPEGKTISKWDGCNNIKLLRFEGATKFQHYIDSFNCNT 245 W ++EAS I G+ ++G T + KWD N+ +L E A +N Sbjct: 264 FIWSISEASIIISGLGFSG--WTDDASPKPKWDRAKNVDILGVELAKSAVQIPLVWNIQV 321 Query: 246 NHFAAEYIYKRLKFLGNRNLSQFFTLL----FLALWHGTRSGYYMTFFNEFLIMLMEKEL 301 + + Y+Y+RL + N + FF LL A+WHG G YM FF + +M+ + Sbjct: 322 STWLRHYVYERL--VQNGKKAGFFQLLATQTVSAVWHGLYPG-YMMFFVQSALMIAGSRV 378 Query: 302 EPIISKTELYNKLWSHNVTKYMLYGLLKMYTIVFMGWSLAPFDLKAFSKWWGVYSSLYYS 361 + N+ ++ + +YT++ + +S F + + + Y S+YY Sbjct: 379 IYRWQQAISPKMAMLRNIMVFINF----LYTVLVLNYSAVGFMVLSLHETLTAYGSVYYI 434 Query: 362 GFVL 365 G ++ Sbjct: 435 GTII 438 >At4g12880.1 68417.m02016 plastocyanin-like domain-containing protein Length = 141 Score = 30.7 bits (66), Expect = 1.8 Identities = 18/53 (33%), Positives = 27/53 (50%), Gaps = 8/53 (15%) Query: 138 LGYLIAFQVGVYVFNVKYMLSDD-FWETSIFY------RHFYCGMWAHFALYK 183 L +L+A + V KY++ D FW +I Y +HFY G W +F Y+ Sbjct: 13 LAFLVAAPIPE-VTAKKYLVGDKKFWNPNINYTLWAQGKHFYVGDWLYFVFYR 64 >At3g14150.1 68416.m01789 (S)-2-hydroxy-acid oxidase, peroxisomal, putative / glycolate oxidase, putative / short chain alpha-hydroxy acid oxidase, putative similar to (S)-2-hydroxy-acid oxidase, peroxisomal (Glycolate oxidase, GOX) (Short chain alpha-hydroxy acid oxidase) [Spinacia oleracea] SWISS-PROT:P05414 Length = 363 Score = 29.1 bits (62), Expect = 5.4 Identities = 16/50 (32%), Positives = 24/50 (48%), Gaps = 4/50 (8%) Query: 209 PEGKTISKWDG--CNNIKLLRFEGATKFQHYIDSFNCNTNHFAAEYIYKR 256 PEG+T + CN I ++ + + F+ S CN F Y+YKR Sbjct: 87 PEGETATAKAAAACNTIMIVSYMSSCTFEEIASS--CNAVRFLQIYVYKR 134 >At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 family protein similar to PHO1 protein [Arabidopsis thaliana] GI:20069032; contains Pfam profiles PF03105: SPX domain, PF03124: EXS family Length = 756 Score = 29.1 bits (62), Expect = 5.4 Identities = 18/56 (32%), Positives = 28/56 (50%) Query: 113 VMDKFPVEKEAKVYEKQALRRLLQGLGYLIAFQVGVYVFNVKYMLSDDFWETSIFY 168 V+ +E + K + QAL LL + F V V FN+ Y S F+ T++F+ Sbjct: 457 VLANLDMETDPKTKDYQALTELLPLFLLIAMFVVLVVPFNIFYRSSRFFFLTTLFH 512 >At3g45940.1 68416.m04971 alpha-xylosidase, putative strong similarity to alpha-xylosidase precursor GI:4163997 from [Arabidopsis thaliana] Length = 868 Score = 28.7 bits (61), Expect = 7.1 Identities = 15/52 (28%), Positives = 26/52 (50%) Query: 221 NNIKLLRFEGATKFQHYIDSFNCNTNHFAAEYIYKRLKFLGNRNLSQFFTLL 272 N IKL+ E T F + +FN NT+ + + +Y L+ + + + LL Sbjct: 184 NGIKLVPNEPYTLFTEDVSAFNLNTDLYGSHPVYMDLRNVSGKAYAHSVLLL 235 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.329 0.142 0.460 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,419,870 Number of Sequences: 28952 Number of extensions: 410373 Number of successful extensions: 1083 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 1073 Number of HSP's gapped (non-prelim): 7 length of query: 392 length of database: 12,070,560 effective HSP length: 82 effective length of query: 310 effective length of database: 9,696,496 effective search space: 3005913760 effective search space used: 3005913760 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.8 bits) S2: 60 (28.3 bits)
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