Silkworm BLAST Result against nematostella

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000809-TA|BGIBMGA000809-PA|undefined
         (627 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_5082| Best HMM Match : Fn_bind (HMM E-Value=6.8)                   156   4e-38
SB_15962| Best HMM Match : DUF528 (HMM E-Value=0.083)                 146   5e-35

>SB_5082| Best HMM Match : Fn_bind (HMM E-Value=6.8)
          Length = 367

 Score =  156 bits (379), Expect = 4e-38
 Identities = 94/227 (41%), Positives = 123/227 (54%), Gaps = 39/227 (17%)

Query: 326 GCASWKWYFPYHYAPFASDFVNICGLSTQFEKGTQPFRPLEQLMGVFPAASAQHVPRPWA 385
           GC SWKWY+PYHYAPFASD                 FR L QL   F   +         
Sbjct: 177 GCPSWKWYYPYHYAPFASD-----------------FRDLGQLKDEFERGTK-------- 211

Query: 386 RLMSDPFSPIIDFYPTDFKIDLNGKKFAWQGVALLPFVDENRLFKALEPYYKELTQAEIQ 445
                P SPIIDFYP DF+IDLNGKKFAWQGVALLPFVDE RL  AL   Y ++++ E +
Sbjct: 212 -----PESPIIDFYPVDFEIDLNGKKFAWQGVALLPFVDEKRLLNALAKVYPDISEDERK 266

Query: 446 RNIRGHDRLYVSTGNQSYEYILGLYEEAQQDLRKLIEDKQKYPYQSEGVRGNVMLSAD-- 503
           RN  G DRL+   G+ ++++  GLYE   + +  L+ D       ++G+ G V    D  
Sbjct: 267 RNSLGKDRLFAREGHPAFDFFKGLYEGPTEPV-DLVTD------LTQGMSGKVEPEKDAH 319

Query: 504 NVLIGGQLPSPVVGLAPVLGNRVVVVRFEDPQYPDEFVFPARRLQRA 550
            +L    +PSPV  L  +  NR + V +EDP + D++V+    L  A
Sbjct: 320 AILPHKIIPSPVTNLRDLRDNRAIGVLYEDPHFSDDYVYKTTVLPNA 366



 Score = 53.6 bits (123), Expect = 5e-07
 Identities = 20/39 (51%), Positives = 36/39 (92%)

Query: 58 GWITDSGDVELDRVQVIMTELGHVEDEIFKRRHQNELSF 96
          G+IT++G V+L++VQ++++ELG+VEDEIFK+R Q+ +++
Sbjct: 27 GYITENGYVDLNKVQLVLSELGYVEDEIFKKRQQDNIAY 65



 Score = 35.1 bits (77), Expect = 0.17
 Identities = 13/26 (50%), Positives = 20/26 (76%)

Query: 182 VRLWEEGFKERYYESKFEVTRNNLEF 207
           +RLWE+G+K RYY +KF V+ ++  F
Sbjct: 127 IRLWEDGWKGRYYSNKFGVSESDEGF 152


>SB_15962| Best HMM Match : DUF528 (HMM E-Value=0.083)
          Length = 1466

 Score =  146 bits (354), Expect = 5e-35
 Identities = 65/130 (50%), Positives = 87/130 (66%), Gaps = 1/130 (0%)

Query: 326 GCASWKWYFPYHYAPFASDFVNICGLSTQFEKGTQPFRPLEQLMGVFPAASAQHVPRPWA 385
           G  SW WY+P+HYAPF SD  +      +F+  ++PF P EQL+GV PAAS Q +P P+ 
Sbjct: 300 GVQSWSWYYPFHYAPFLSDIKDFSHYELKFDM-SKPFLPFEQLLGVLPAASKQLLPAPFQ 358

Query: 386 RLMSDPFSPIIDFYPTDFKIDLNGKKFAWQGVALLPFVDENRLFKALEPYYKELTQAEIQ 445
            LM    SPIID+YP +FK DLNGK+  W+ V L+PF+DE RL +A++P Y++LT+ E  
Sbjct: 359 NLMIMEQSPIIDYYPLNFKTDLNGKQQEWEAVVLIPFIDEKRLLEAMKPLYRQLTKEEKT 418

Query: 446 RNIRGHDRLY 455
           RN  G   LY
Sbjct: 419 RNTHGPCHLY 428



 Score = 80.2 bits (189), Expect = 5e-15
 Identities = 35/86 (40%), Positives = 51/86 (59%)

Query: 1   MPNLPFPYDFERALDDWVFMCFFVGNDFLPHLPSLEIREGAVDRLVNLYKKCVYKTKGWI 60
           + +LPF YD ER +DDW+ M F VGNDF+PHLP + I   A+  L  +YK+ +    G++
Sbjct: 28  LDSLPFEYDLERIIDDWILMGFLVGNDFIPHLPYMHINHDALPMLYQIYKEIMPSLDGYL 87

Query: 61  TDSGDVELDRVQVIMTELGHVEDEIF 86
            D G + L R +  + EL   + E F
Sbjct: 88  HDGGTLCLPRFEKYLKELSKFDREKF 113


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.323    0.140    0.451 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,110,249
Number of Sequences: 59808
Number of extensions: 809111
Number of successful extensions: 1399
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1391
Number of HSP's gapped (non-prelim): 6
length of query: 627
length of database: 16,821,457
effective HSP length: 86
effective length of query: 541
effective length of database: 11,677,969
effective search space: 6317781229
effective search space used: 6317781229
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 63 (29.5 bits)