Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000808-TA|BGIBMGA000808-PA|IPR004859|Putative 5-3
exonuclease
         (322 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomy...   352   3e-98
SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb...   259   4e-70
SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subuni...    33   0.072
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe...    27   3.6  
SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces...    27   4.7  
SPAC8C9.06c |||mitochondrial translation regulator |Schizosaccha...    27   4.7  
SPBC31F10.14c |hip3|hir3|HIRA interacting protein Hip3|Schizosac...    27   4.7  
SPAC637.12c |mst1||histone acetyltransferase Mst1|Schizosaccharo...    27   4.7  
SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family |Schizos...    26   8.3  

>SPAC26A3.12c |dhp1||5'-3' exoribonuclease Dhp1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 991

 Score =  352 bits (866), Expect = 3e-98
 Identities = 178/319 (55%), Positives = 225/319 (70%), Gaps = 15/319 (4%)

Query: 1   MGVPAFFRWLSRKYPSVIVECVEQKPTDV--DGQLVYVDSSLPNPNGVEFDNLYLDMNGI 58
           MGVPA FR LSRK+  VI   +E  PT+   DG  +  D SLPNPNGVE DNLYLDMNGI
Sbjct: 1   MGVPALFRLLSRKFAKVITPVIEA-PTEKLPDGTEIEPDLSLPNPNGVECDNLYLDMNGI 59

Query: 59  IHPCTHPEDKPAPKDEDEMMVAIFECIDRLFRIVRPRKLLYMAIDGVAPRAKMNQQRSRR 118
           +HPC+HPED+PAP+ EDEMMVA+FE  DR+  +VRPR+LL++AIDGVAPRAKMNQQRSRR
Sbjct: 60  VHPCSHPEDRPAPETEDEMMVAVFEYTDRILAMVRPRQLLFIAIDGVAPRAKMNQQRSRR 119

Query: 119 FRASKETQEKIEEVARIRNELQAKGAYLPPERPKEAHFDSNCITPGTPFMDRLSKCLHYY 178
           FR+S+E   K EE+     E + +G  +     K+  +DSNCITPGTPFMD L+K L YY
Sbjct: 120 FRSSREAALKEEELQAFIEEAKQQGIPIDENATKKKSWDSNCITPGTPFMDTLAKSLRYY 179

Query: 179 IHDRLNNDPGWKGIKVILSDANVPGEGEHKIMDYIRRQRAQPDHDPNTQHVLCGADADLI 238
           I ++LN+DP W+ ++ ILSDA+VPGEGEHKIM++IR QR +P++DPNT HV+ G DADLI
Sbjct: 180 IINKLNSDPCWRNVRFILSDASVPGEGEHKIMEFIRSQRVKPEYDPNTHHVVYGLDADLI 239

Query: 239 MLGLATHEPNFTIIREEFKPNKPRPCDVCGQLGHEMKECTGTN--PDASVVRSDPAFGNQ 296
           MLGLATHEP+F ++RE+    +       G      +E  G     D S     P    +
Sbjct: 240 MLGLATHEPHFRVLREDVFFQQ-------GSTKKTKEERLGIKRLDDVSETNKVPV---K 289

Query: 297 DNFIFVRLSVLREYLEREL 315
             FI++ +S+LREYLE EL
Sbjct: 290 KPFIWLNVSILREYLEVEL 308


>SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1328

 Score =  259 bits (634), Expect = 4e-70
 Identities = 135/257 (52%), Positives = 175/257 (68%), Gaps = 33/257 (12%)

Query: 1   MGVPAFFRWLSRKYPSVIVECVEQKPTDVDGQLVYVDSSLPNPNGVEFDNLYLDMNGIIH 60
           MG+P FFRW+S +YP     C          QL+  D  +P     EFDNLYLDMNGI+H
Sbjct: 1   MGIPKFFRWMSERYPL----C---------SQLIENDR-IP-----EFDNLYLDMNGILH 41

Query: 61  PCTHPEDKPA--PKDEDEMMVAIFECIDRLFRIVRPRKLLYMAIDGVAPRAKMNQQRSRR 118
            CTH  D  +  P  E+EM +AIF  I+ LF  ++P+KLLYMA+DG APRAKMNQQRSRR
Sbjct: 42  NCTHKNDDHSSPPLPEEEMYIAIFNYIEHLFEKIKPKKLLYMAVDGCAPRAKMNQQRSRR 101

Query: 119 FRASKETQEKIEEVARIRNELQAKGAYLPPERPKEAHFDSNCITPGTPFMDRLSKCLHYY 178
           FR +K+  +     AR++ E    G   P E+     FDSNCITPGT FM+R+S+ L+Y+
Sbjct: 102 FRTAKDAHD-----ARLKAE--RNGEDFPEEQ-----FDSNCITPGTTFMERVSRQLYYF 149

Query: 179 IHDRLNNDPGWKGIKVILSDANVPGEGEHKIMDYIRRQRAQPDHDPNTQHVLCGADADLI 238
           IH ++ ND  W+ I+VI S  + PGEGEHKIM+YIR Q+AQP ++PNT+H L G DADLI
Sbjct: 150 IHKKVTNDSQWQNIEVIFSGHDCPGEGEHKIMEYIRTQKAQPSYNPNTRHCLYGLDADLI 209

Query: 239 MLGLATHEPNFTIIREE 255
           MLGL +H+P+F ++REE
Sbjct: 210 MLGLLSHDPHFCLLREE 226


>SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subunit
           8|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 230

 Score = 32.7 bits (71), Expect = 0.072
 Identities = 17/45 (37%), Positives = 25/45 (55%), Gaps = 1/45 (2%)

Query: 98  LYMAIDGVAPRAKMNQQRSRRFRASKETQEKIEEVARIRNELQAK 142
           LY   D    R K  +QRS  FRASK   E++E    +RN+++ +
Sbjct: 163 LYGDRDSFQSRLKQKKQRSE-FRASKREMERLERDQELRNKIEER 206


>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 27.1 bits (57), Expect = 3.6
 Identities = 11/27 (40%), Positives = 17/27 (62%)

Query: 225 NTQHVLCGADADLIMLGLATHEPNFTI 251
           N +H++  AD  L+ LG AT  P+F +
Sbjct: 333 NGRHIILLADGRLVNLGCATGHPSFVM 359


>SPAC13D6.02c |byr3||zinc finger protein Byr3|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 179

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 10/20 (50%), Positives = 14/20 (70%), Gaps = 1/20 (5%)

Query: 260 KPRP-CDVCGQLGHEMKECT 278
           +P P C  CG+ GH+ +ECT
Sbjct: 14  RPGPRCYNCGENGHQARECT 33



 Score = 26.2 bits (55), Expect = 6.2
 Identities = 9/27 (33%), Positives = 16/27 (59%), Gaps = 1/27 (3%)

Query: 257 KPNKPRPCDVCGQLGHEMKEC-TGTNP 282
           +P + + C  CG  GH +++C +  NP
Sbjct: 53  EPQQEKTCYACGTAGHLVRDCPSSPNP 79


>SPAC8C9.06c |||mitochondrial translation regulator
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 931

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 12/37 (32%), Positives = 22/37 (59%)

Query: 10  LSRKYPSVIVECVEQKPTDVDGQLVYVDSSLPNPNGV 46
           LSR++  +I++  +Q     D   V  D ++P+P+GV
Sbjct: 742 LSRQFWDLILQVAQQNFITTDIAAVVDDKNIPSPSGV 778


>SPBC31F10.14c |hip3|hir3|HIRA interacting protein
           Hip3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1630

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 13/45 (28%), Positives = 23/45 (51%)

Query: 204 EGEHKIMDYIRRQRAQPDHDPNTQHVLCGADADLIMLGLATHEPN 248
           E  H+++     + + PD D +T     GA + +I++ LA H  N
Sbjct: 409 EDYHQLLVNFPSEGSIPDLDVSTDSAANGAFSKMILIDLAMHSAN 453


>SPAC637.12c |mst1||histone acetyltransferase
           Mst1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 463

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 13/30 (43%), Positives = 20/30 (66%), Gaps = 1/30 (3%)

Query: 127 EKIEEVARIRNELQAKGA-YLPPERPKEAH 155
           ++++E   I N   +KG  Y PPE+PK+AH
Sbjct: 61  KRLDEWITIDNIDLSKGIEYPPPEKPKKAH 90


>SPBP8B7.15c |||ubiquitin-protein ligase E3 RBBP6 family
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 482

 Score = 25.8 bits (54), Expect = 8.3
 Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 5/30 (16%)

Query: 258 PNKPRP----CDVCGQLGHEMKECTGTNPD 283
           P+KP P    C  CGQ GH ++ C  TN D
Sbjct: 175 PDKPPPPGYICYRCGQKGHWIQACP-TNAD 203


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.321    0.139    0.428 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,628,889
Number of Sequences: 5004
Number of extensions: 72070
Number of successful extensions: 181
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 164
Number of HSP's gapped (non-prelim): 18
length of query: 322
length of database: 2,362,478
effective HSP length: 73
effective length of query: 249
effective length of database: 1,997,186
effective search space: 497299314
effective search space used: 497299314
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 54 (25.8 bits)