Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000802-TA|BGIBMGA000802-PA|IPR003191|Guanylate-binding
protein
         (528 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g38840.1 68415.m04772 guanylate-binding family protein simila...    47   3e-05
At5g46070.1 68418.m05665 guanylate-binding family protein contai...    41   0.002
At4g08620.1 68417.m01419 sulfate transporter identical to sulfat...    33   0.47 
At2g40000.1 68415.m04915 expressed protein                             29   5.8  

>At2g38840.1 68415.m04772 guanylate-binding family protein similar
           to SP|Q01514 Interferon-induced guanylate-binding
           protein 1 (Guanine nucleotide-binding protein 1)
           (Interferon-gamma inducible protein MAG-1) {Mus
           musculus}; contains Pfam profile PF02263:
           Guanylate-binding protein, N-terminal domain
          Length = 679

 Score = 47.2 bits (107), Expect = 3e-05
 Identities = 45/153 (29%), Positives = 69/153 (45%), Gaps = 27/153 (17%)

Query: 16  VVVPGPEHTFXXXXXXXXXXXXXXXIKDRAVVIISVAGAFRKGKSFLLDFFLRYLQHKYV 75
           +V P P HT                    AV +I   G +R GKSFLL+  L    +   
Sbjct: 33  IVEPAPGHTKLQLSREGLEAISRITTPISAVAVI---GPYRSGKSFLLNQLLSLSCY--- 86

Query: 76  LGESGGEWIGVEDEPLEGFKWRGGSERETTGLLLWSQPFKATLANGEKVVIFLMDTQGTF 135
                           EGF      + +T G+ +W  P +  + +G K  +  +DT+G F
Sbjct: 87  ----------------EGFGVGHMRDTKTKGIWVWGTPLELEI-DGVKTSVIYLDTEG-F 128

Query: 136 DS--QSTVRDNATVFALSTLISSVQIYNLSQNI 166
           +S  +S V D+  +FAL+T++SSV IYNL + +
Sbjct: 129 ESVGKSNVYDDR-IFALATVMSSVLIYNLPETV 160



 Score = 41.1 bits (92), Expect = 0.002
 Identities = 33/158 (20%), Positives = 69/158 (43%), Gaps = 14/158 (8%)

Query: 233 ELQNLREYVTRIFDEIACFLMPHPGLKVATNPDFNGKLADIS-PEFKDSLKQLLPMLLAP 291
           ++  +R+ +  + D    F +P P L      D   +  D +    +D LK+L+  ++ P
Sbjct: 307 QVNQIRDSLAIMGDNSTAFSLPQPHLMRTKLCDLKDEDLDSTYVARRDQLKKLVASIIRP 366

Query: 292 ENLVPKLIEGQKVKAKDLLLYFKTYMKVFNGTELPEPRTILAATSEANNLSAVAEAREVY 351
                K+++G+ +  K+ + + +  +   N  E+P   +++    E  N   V    ++Y
Sbjct: 367 -----KIVQGKTLNGKEFISFLEQILDALNKGEIPSTGSLV----EVFNKDIVERCVKLY 417

Query: 352 ETLMEEICGGTRPYVQPRQLEEEHHRVMNKAIHAFDSK 389
              M  +    R  +    L+  H    N+AI AFD++
Sbjct: 418 NEKMVRV----RLPMSEESLQSAHEMAHNEAIKAFDAQ 451


>At5g46070.1 68418.m05665 guanylate-binding family protein contains
           Pfam domains PF02263: Guanylate-binding protein,
           N-terminal domain and PF02841: Guanylate-binding
           protein, C-terminal domain
          Length = 1060

 Score = 40.7 bits (91), Expect = 0.002
 Identities = 76/350 (21%), Positives = 132/350 (37%), Gaps = 55/350 (15%)

Query: 48  IISVAGAFRKGKSFLLDFFLRYLQHKYVLGESGGEWIGVEDEPLEGFKWRGGSERETTGL 107
           ++SV G  R+GKSF+L+          +LG S G  +    +P             T GL
Sbjct: 72  VVSVCGRARQGKSFILN---------QLLGRSNGFQVASTHKPC------------TKGL 110

Query: 108 LLWSQPFKATLANGEKVVIFLMDTQG--TFDSQSTVRDNATVFALSTLISSVQIYNLSQN 165
            LWS P K T  +G +  + L+D++G   +D      D A++  LS L++ +        
Sbjct: 111 WLWSSPIKRTALDGTEYNLLLLDSEGIDAYDQTMGGIDEASLDRLS-LVTQM-------- 161

Query: 166 IEEDDLQHLQLFTDYGQLAQESSSGRPFQRLQFLVRDWSFPYDHPYGAXXXXXXXXXXXX 225
                 +H+++    G  +  S  G+      +L+RD+                      
Sbjct: 162 -----TKHIRVKAS-GGTSSRSELGQFSPIFVWLLRDFYLDLVEDNRKISPRDYLEIALR 215

Query: 226 IHEGQHPEL---QNLREYVTRIFDEIACFLMPHP--GLKVATNPDFNGKLADISPEFKDS 280
             +G   ++     +R+ +  +F +  CF +  P    K     D    L  + PEF   
Sbjct: 216 PVQGSGGDIGAKNEIRDSIRALFPDRECFTLVRPLNNEKDLQRLD-QISLEKLRPEFGAG 274

Query: 281 LKQLLPMLLAPENLVPKLIEGQKVKAKDLLLYFKTYMKVFNGTELPEPRTILAATSEANN 340
           L      +   E   PK + G  +    L+   ++Y+   N   +P   TI ++      
Sbjct: 275 LDAFTKFVF--EKTRPKQLGGTVMTGPILVGITQSYLDALNNGAVP---TITSSWQSVEE 329

Query: 341 LSAVAEAREVYETLMEEICGGTRPYVQPRQ--LEEEHHRVMNKAIHAFDS 388
                E R  Y++ +E           P +  L EEH   + KA+  F+S
Sbjct: 330 ----TECRRAYDSGVEAYMAAFDQSKAPEEGALREEHEEAVRKALAIFNS 375


>At4g08620.1 68417.m01419 sulfate transporter identical to sulfate
           transporter [Arabidopsis thaliana] GI:3777483
          Length = 649

 Score = 33.1 bits (72), Expect = 0.47
 Identities = 18/44 (40%), Positives = 25/44 (56%), Gaps = 1/44 (2%)

Query: 52  AGAFRKGKSFL-LDFFLRYLQHKYVLGESGGEWIGVEDEPLEGF 94
           AG F+ G  FL L F + +L H  V+G  GG  I +  + L+GF
Sbjct: 177 AGIFQAGLGFLRLGFLIDFLSHAAVVGFMGGAAITIALQQLKGF 220


>At2g40000.1 68415.m04915 expressed protein
          Length = 435

 Score = 29.5 bits (63), Expect = 5.8
 Identities = 27/110 (24%), Positives = 46/110 (41%), Gaps = 5/110 (4%)

Query: 274 SPEFKDSLKQLLPMLLAPENLVPKLIEGQKVKAKDLLLYFKTYMKVFNGTELPEPRTILA 333
           SP+    L   +P   +P  +VP +       A  L   ++ Y+++    +L   R    
Sbjct: 18  SPKLSSKLHVTIP---SPFKIVP-VSSPISCSAPALCSAYELYLRLPELRKLWSSRDFPQ 73

Query: 334 ATSEANNLSAVAEAREVYETLMEEICGGTRPYVQPRQLEEEHHRVMNKAI 383
            TSE   L    +A E+   L+  +C  TRPY+  R+       ++ K I
Sbjct: 74  WTSEPI-LKPALQALEISFRLVFAVCSDTRPYINHREWNRRLDSLITKQI 122


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.319    0.135    0.396 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,095,791
Number of Sequences: 28952
Number of extensions: 434031
Number of successful extensions: 856
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 849
Number of HSP's gapped (non-prelim): 7
length of query: 528
length of database: 12,070,560
effective HSP length: 84
effective length of query: 444
effective length of database: 9,638,592
effective search space: 4279534848
effective search space used: 4279534848
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 62 (29.1 bits)