BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000800-TA|BGIBMGA000800-PA|IPR011012|Longin-like, IPR000804|Clathrin adaptor complex, small chain (142 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 177 7e-46 SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyc... 150 9e-38 SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosac... 103 1e-23 SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyc... 43 2e-05 SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 43 2e-05 SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 36 0.003 SPAC6C3.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 1.1 SPAC24C9.15c |spn5|mde9, meu28|septin Spn5|Schizosaccharomyces p... 27 1.1 SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosac... 26 1.9 SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12 |Schizos... 26 2.6 SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 2.6 SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb... 25 3.4 SPAC959.03c |||U3 snoRNP-associated protein Utp7|Schizosaccharom... 25 3.4 SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe... 25 3.4 SPBC146.11c |mug97|meu33|meiotically upregulated gene Mug97|Schi... 25 4.5 SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe... 24 7.8 >SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 143 Score = 177 bits (430), Expect = 7e-46 Identities = 81/143 (56%), Positives = 108/143 (75%), Gaps = 1/143 (0%) Query: 1 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAK-HTNFVEFRNFKIVY 59 MI+FILIQNR GK RL+K+Y+ FDDDEK +L +H +++ R+ K NF+E+ N K+VY Sbjct: 1 MIQFILIQNRHGKNRLSKYYVPFDDDEKVRLKARIHQLISQRNQKFQANFLEWENSKLVY 60 Query: 60 RRYAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFL 119 RRYAGLYFC CVD DN+L LE IH FVE+L+ +F NVCELDL+FNFYKV ++DE+ L Sbjct: 61 RRYAGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEIIL 120 Query: 120 AGEIRETSQTKVLKQLLMLNSLE 142 GEI E+++ VL+++ L LE Sbjct: 121 GGEIGESNKKSVLERIEALEKLE 143 >SPAP27G11.06c |||AP-1 adaptor complex subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 162 Score = 150 bits (363), Expect = 9e-38 Identities = 69/135 (51%), Positives = 95/135 (70%) Query: 2 IRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIVYRR 61 I+F L+ +R GK RLAKW+ E+ K+I +V ++V R K NFVE++ KIVYRR Sbjct: 3 IKFFLLVSRQGKVRLAKWFNTLSIKERAKIIRDVSSLVITRKPKMCNFVEYKGEKIVYRR 62 Query: 62 YAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAG 121 YA L+F ++ +DN L LE IH FVE L++YF NVCELDL+FNF K Y V++E+ LAG Sbjct: 63 YASLFFVCGIEQDDNELIILEVIHKFVECLDKYFGNVCELDLIFNFEKAYYVMEELLLAG 122 Query: 122 EIRETSQTKVLKQLL 136 E++E+S+T VL +L Sbjct: 123 ELQESSKTNVLSAVL 137 >SPAC30D11.05 |aps3||AP-3 adaptor complex subunit Aps3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 165 Score = 103 bits (246), Expect = 1e-23 Identities = 53/136 (38%), Positives = 81/136 (59%), Gaps = 4/136 (2%) Query: 1 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNF----K 56 MI + I N GK RL K+Y D+ +QKLI +++A V+ R NF+E + Sbjct: 1 MIYAVFIFNNKGKPRLTKFYTPIDESIQQKLIGDIYAAVSTRPPTACNFLESNLIAGKNR 60 Query: 57 IVYRRYAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDE 116 I+YR+YA LYF VD ++ L L+ I FVE L+ F+NVCELDLVF F +++ ++ E Sbjct: 61 IIYRQYATLYFVFVVDEGESELGILDLIQVFVEALDRCFNNVCELDLVFKFQEIHAILAE 120 Query: 117 MFLAGEIRETSQTKVL 132 + G + ET+ +++ Sbjct: 121 VVSGGLVLETNLNEIV 136 >SPCC285.08 |ret2||coatomer delta subunit Ret2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 240 Score = 43.2 bits (97), Expect = 2e-05 Identities = 29/133 (21%), Positives = 60/133 (45%), Gaps = 2/133 (1%) Query: 5 ILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIVYRRYAG 64 + I NR GK +++ + + L+ A+V+ + T VE N + VY+ Sbjct: 6 VSIVNRGGKAIISRQFREMSRVRVESLLSSFPALVSEKSQNTT--VESDNVRFVYQPLDE 63 Query: 65 LYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIR 124 LY + ++ N L ++ +H +V+ ++ E +++ ++++T DE G Sbjct: 64 LYIVLITNLQSNILQDIDTLHLLSQVVTSICSSLEEREILEYAFEIFTAFDEATSLGYRD 123 Query: 125 ETSQTKVLKQLLM 137 S T++ L M Sbjct: 124 NVSLTQIKTYLEM 136 >SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 446 Score = 42.7 bits (96), Expect = 2e-05 Identities = 34/127 (26%), Positives = 57/127 (44%), Gaps = 5/127 (3%) Query: 1 MIRFILIQNRAGKTRLAKWYMNFDDDEKQKLIEEVH-AVVTVRDAKHTNFVEFRNFKIVY 59 MI + I N G T + K F D K+ + E A++T D +H V + +Y Sbjct: 1 MISGLFIFNLKGDTLICK---TFRHDLKKSVTEIFRVAILTNTDYRHP-IVSIGSSTYIY 56 Query: 60 RRYAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFL 119 ++ LY N N + LE + + ++ L YF + E + N ++ ++DEM Sbjct: 57 TKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKDNVSFIFELLDEMID 116 Query: 120 AGEIRET 126 G I+ T Sbjct: 117 YGIIQTT 123 >SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 426 Score = 35.5 bits (78), Expect = 0.003 Identities = 20/83 (24%), Positives = 41/83 (49%), Gaps = 1/83 (1%) Query: 58 VYRRYAGLYFCICVDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEM 117 +Y + +Y +N + + L + +V +YF + E + NF VY ++DE+ Sbjct: 58 IYIHHNDVYLLALSKMNSDAMEMLVFLRKMADVFIDYFKELQEESIRDNFVLVYELLDEI 117 Query: 118 FLAGEIRETSQTKVLKQLLMLNS 140 G +T++TK+L++ + S Sbjct: 118 MDFG-FPQTTETKILQEYITQTS 139 >SPAC6C3.05 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 269 Score = 27.1 bits (57), Expect = 1.1 Identities = 14/49 (28%), Positives = 23/49 (46%), Gaps = 1/49 (2%) Query: 71 VDVNDNNLCYLEAIHNFVEVLNEYFHNVCELDLVFNFY-KVYTVVDEMF 118 ++ +DN+L YL L YF + + V N K TV+D ++ Sbjct: 111 INASDNSLAYLSKPEESYNFLTIYFSTLLSYEKVENLIKKSLTVIDSLY 159 >SPAC24C9.15c |spn5|mde9, meu28|septin Spn5|Schizosaccharomyces pombe|chr 1|||Manual Length = 464 Score = 27.1 bits (57), Expect = 1.1 Identities = 11/31 (35%), Positives = 17/31 (54%) Query: 72 DVNDNNLCYLEAIHNFVEVLNEYFHNVCELD 102 D +DN+ C+ N + LN YF N ++D Sbjct: 180 DKSDNSNCWRPIATNLLHRLNAYFQNEVKMD 210 >SPCC18B5.11c |cds1||replication checkpoint kinase Cds1|Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 26.2 bits (55), Expect = 1.9 Identities = 17/52 (32%), Positives = 25/52 (48%) Query: 85 HNFVEVLNEYFHNVCELDLVFNFYKVYTVVDEMFLAGEIRETSQTKVLKQLL 136 H V +E F N EL +V + + ++D + G I E +LKQLL Sbjct: 228 HPGVVQCHEIFENDDELFIVMEYVEGGDLMDFLIANGSIDEQDCKPLLKQLL 279 >SPBC32F12.05c |cwf12||complexed with Cdc5 protein Cwf12 |Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 25.8 bits (54), Expect = 2.6 Identities = 10/34 (29%), Positives = 17/34 (50%) Query: 13 KTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKH 46 K RL WY + ++ L+E+ A + + KH Sbjct: 141 KRRLDAWYFGYIPPAQESLLEDFEAKIEEQQHKH 174 >SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1233 Score = 25.8 bits (54), Expect = 2.6 Identities = 12/56 (21%), Positives = 26/56 (46%) Query: 6 LIQNRAGKTRLAKWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIVYRR 61 L+ N GK + ++D + LIE+ + + + FVE+++ + R+ Sbjct: 651 LLLNEMGKLFQVELNFSYDSPDVNLLIEQFYEITSYESNYDDAFVEYKDATVANRK 706 >SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 25.4 bits (53), Expect = 3.4 Identities = 23/86 (26%), Positives = 35/86 (40%), Gaps = 6/86 (6%) Query: 39 VTVRDAKHTNFVEFRNFKIVYRRYAGLYFCICVDVNDNNLCYLEAIHNF---VEVLNEYF 95 VTV + N F + + IC NDNNL L+ H ++VL +Y Sbjct: 181 VTVHETGFGNIENLATFSTKFVALSSHKNYICGLTNDNNLYILQHSHQAGTKLKVLGKYH 240 Query: 96 HNVCELDLVFNFYKV---YTVVDEMF 118 + E + +F + Y VV +F Sbjct: 241 VSSIEKQVAIHFQQSKEGYEVVHVVF 266 >SPAC959.03c |||U3 snoRNP-associated protein Utp7|Schizosaccharomyces pombe|chr 1|||Manual Length = 520 Score = 25.4 bits (53), Expect = 3.4 Identities = 15/49 (30%), Positives = 25/49 (51%), Gaps = 2/49 (4%) Query: 18 KWYMNFDDDEKQKLIEEVHAVVTVRDAKHTNFVEFRNFKIVYRRYAGLY 66 K +++ D KL+ E+H TVRD K F + F + ++Y +Y Sbjct: 122 KGHISAFDWRTGKLLTELHLRETVRDVKW--FHNHQYFAVAQKKYVYVY 168 >SPAC140.04 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 295 Score = 25.4 bits (53), Expect = 3.4 Identities = 15/49 (30%), Positives = 30/49 (61%), Gaps = 4/49 (8%) Query: 17 AKWYMNFDDDEKQKLI---EEVHAVVTVRDAKHT-NFVEFRNFKIVYRR 61 A + +FD+ E+++ + +E+HA VT + K+T + + R+ K+ RR Sbjct: 224 AFYQFSFDEKEREEQLLSLKEIHAKVTQQQRKNTEDVLTLRDKKLEERR 272 >SPBC146.11c |mug97|meu33|meiotically upregulated gene Mug97|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 25.0 bits (52), Expect = 4.5 Identities = 7/17 (41%), Positives = 13/17 (76%) Query: 17 AKWYMNFDDDEKQKLIE 33 ++WY+ FD D+ +L+E Sbjct: 178 SRWYLPFDKDDDNQLVE 194 >SPBC1105.04c |cbp1|abp1|CENP-B homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 24.2 bits (50), Expect = 7.8 Identities = 10/33 (30%), Positives = 18/33 (54%), Gaps = 1/33 (3%) Query: 4 FILIQNRAGKTRLAKWYM-NFDDDEKQKLIEEV 35 F +QNR+G+ L +W+ F D Q + ++ Sbjct: 21 FFQLQNRSGQQDLIEWFREKFGKDISQPSVSQI 53 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.328 0.142 0.424 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 664,020 Number of Sequences: 5004 Number of extensions: 27625 Number of successful extensions: 120 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 104 Number of HSP's gapped (non-prelim): 17 length of query: 142 length of database: 2,362,478 effective HSP length: 66 effective length of query: 76 effective length of database: 2,032,214 effective search space: 154448264 effective search space used: 154448264 T: 11 A: 40 X1: 15 ( 7.1 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 40 (21.8 bits) S2: 50 (24.2 bits)
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