Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000786-TA|BGIBMGA000786-PA|IPR005225|Small GTP-binding
protein domain, IPR006689|ARF/SAR superfamily,
IPR006688|ADP-ribosylation factor, IPR006687|GTP-binding protein SAR1,
IPR003579|Ras small GTPase, Rab type
(184 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 143 2e-35
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 114 8e-27
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 106 2e-24
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 58 6e-10
SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr 1||... 39 4e-04
SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr... 38 0.001
SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 37 0.002
SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr 1|... 36 0.003
SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr 1||... 33 0.034
SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|ch... 32 0.059
SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr 1|||... 31 0.078
SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|ch... 31 0.14
SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 27 1.7
SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr... 27 1.7
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 27 2.2
SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||... 27 2.2
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 26 2.9
SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyce... 25 5.1
SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom... 25 5.1
SPCC1223.13 |cbf12||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Ma... 25 5.1
SPCC825.01 |||ribosome biogenesis ATPase, Arb family |Schizosacc... 25 8.9
SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 25 8.9
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 143 bits (346), Expect = 2e-35
Identities = 68/169 (40%), Positives = 96/169 (56%), Gaps = 1/169 (0%)
Query: 17 ISIKVRGLDNAGKTTILKRFNGEPIDTISPTLGFNIKTLEHKGFKLNIWDVGGQKSLRSY 76
+ + + GLDNAGKTTILK E ++ +SPT GF I+TLE +G + IWD+GGQK+LR++
Sbjct: 17 VRVLLLGLDNAGKTTILKCLLNEDVNEVSPTFGFQIRTLEVEGLRFTIWDIGGQKTLRNF 76
Query: 77 WKNYFESTXXXXXXXXXXXXXRLSACXXXXXXXXXXXXXXXXXXXXXXNKCDLPGALSLQ 136
WKNYFEST RL C NK D+ GALS +
Sbjct: 77 WKNYFESTEAIIWVVDSLDDLRLEECRNTLQELLVEEKLLFTSILVLANKSDVSGALSSE 136
Query: 137 QIREALDLDSIKSHHWRIVRCSAVTGENLLEGIDWMLDDIAS-RIFTLD 184
+I + L++ KS HWRI SA+TG N+ + I W+ +D+ ++ T+D
Sbjct: 137 EISKILNISKYKSSHWRIFSVSALTGLNIKDAISWLANDLKEIKLGTID 185
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 114 bits (274), Expect = 8e-27
Identities = 59/155 (38%), Positives = 80/155 (51%), Gaps = 2/155 (1%)
Query: 19 IKVRGLDNAGKTTILKRFN-GEPIDTISPTLGFNIKTLEHKGFKLNIWDVGGQKSLRSYW 77
I + GLD AGKTTIL + + + TI PT+GFN++T+ +K K N+WDVGGQ +R W
Sbjct: 24 ILMLGLDAAGKTTILYKLKLNQSVVTI-PTVGFNVETVTYKNIKFNVWDVGGQDKIRPLW 82
Query: 78 KNYFESTXXXXXXXXXXXXXRLSACXXXXXXXXXXXXXXXXXXXXXXNKCDLPGALSLQQ 137
++YF T R+S NK DLPGALS Q
Sbjct: 83 RHYFTGTKGLIFVVDSADSNRISEARQELHRIISDREMRDCLLLVLANKQDLPGALSPAQ 142
Query: 138 IREALDLDSIKSHHWRIVRCSAVTGENLLEGIDWM 172
I + L LD +K W + A+TG+ LLEG+ W+
Sbjct: 143 ITDVLQLDKLKDRLWNVQPTCALTGDGLLEGLAWL 177
>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 180
Score = 106 bits (255), Expect = 2e-24
Identities = 52/158 (32%), Positives = 82/158 (51%), Gaps = 2/158 (1%)
Query: 23 GLDNAGKTTILKRFN-GEPIDTISPTLGFNIKTLEHKGFKLNIWDVGGQKSLRSYWKNYF 81
GLD AGKTTIL + GE + TI PT+GFN++T+E++ +WDVGGQ +R W++YF
Sbjct: 24 GLDAAGKTTILYKLKLGEIVTTI-PTIGFNVETVEYRNISFTVWDVGGQDKIRPLWRHYF 82
Query: 82 ESTXXXXXXXXXXXXXRLSACXXXXXXXXXXXXXXXXXXXXXXNKCDLPGALSLQQIREA 141
++T R+S NK DLP A++ +I +
Sbjct: 83 QNTQGIIFVVDSNDRERISEAHEELQRMLNEDELRDALLLVFANKQDLPNAMNAAEITDK 142
Query: 142 LDLDSIKSHHWRIVRCSAVTGENLLEGIDWMLDDIASR 179
L L S++ W I A +G+ L EG++W+ ++ ++
Sbjct: 143 LGLHSLRHRQWYIQATCATSGDGLYEGLEWLSTNLKNQ 180
>SPBC31F10.06c |sar1||ADP-ribosylation factor
Sar1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 190
Score = 58.4 bits (135), Expect = 6e-10
Identities = 34/122 (27%), Positives = 49/122 (40%)
Query: 23 GLDNAGKTTILKRFNGEPIDTISPTLGFNIKTLEHKGFKLNIWDVGGQKSLRSYWKNYFE 82
GLDNAGKTT+L + + + PTL + L + +D+GG + R W++YF
Sbjct: 27 GLDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELAIGNVRFTTFDLGGHQQARRLWRDYFP 86
Query: 83 STXXXXXXXXXXXXXRLSACXXXXXXXXXXXXXXXXXXXXXXNKCDLPGALSLQQIREAL 142
RLS NK D PGA+S +++ AL
Sbjct: 87 EVNGIVYLVDCCDFERLSESKAELDALLAMEELARVPFLILGNKIDAPGAISEDELKAAL 146
Query: 143 DL 144
L
Sbjct: 147 GL 148
>SPAC1B3.11c |ypt4||GTPase Ypt4|Schizosaccharomyces pombe|chr
1|||Manual
Length = 234
Score = 39.1 bits (87), Expect = 4e-04
Identities = 23/74 (31%), Positives = 37/74 (50%), Gaps = 9/74 (12%)
Query: 17 ISIKVRGLDNAGKTTILKRF-NGEPIDTISPTLGFNIKTL--------EHKGFKLNIWDV 67
+ I + G GK+ +L+RF + D +S T+G + + + K KL IWD
Sbjct: 10 VKIVLAGPSGTGKSCLLQRFVKNQWDDQVSHTVGIDFASRIISVGMGNQQKRIKLQIWDT 69
Query: 68 GGQKSLRSYWKNYF 81
GQ+ RS +NY+
Sbjct: 70 AGQEKFRSVARNYY 83
>SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 201
Score = 37.5 bits (83), Expect = 0.001
Identities = 22/67 (32%), Positives = 35/67 (52%), Gaps = 5/67 (7%)
Query: 23 GLDNAGKTTILKRFNGEPID-TISPTLGFNI--KT--LEHKGFKLNIWDVGGQKSLRSYW 77
G + GKT+++ RF + D T T+G + KT LE + +L +WD GQ+ RS
Sbjct: 18 GEQSVGKTSLITRFMYDQFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLI 77
Query: 78 KNYFEST 84
+Y +
Sbjct: 78 PSYIRDS 84
>SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 203
Score = 36.7 bits (81), Expect = 0.002
Identities = 23/64 (35%), Positives = 34/64 (53%), Gaps = 5/64 (7%)
Query: 23 GLDNAGKTTILKRFNGEPI-DTISPTLG--FNIKT--LEHKGFKLNIWDVGGQKSLRSYW 77
G GK+ +L RF + ++ T+G F I+T LE K KL IWD GQ+ R+
Sbjct: 15 GDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTFELEGKTVKLQIWDTAGQERFRTIT 74
Query: 78 KNYF 81
+Y+
Sbjct: 75 SSYY 78
>SPAC9E9.07c |ypt2||GTPase Ypt2 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 200
Score = 36.3 bits (80), Expect = 0.003
Identities = 23/70 (32%), Positives = 36/70 (51%), Gaps = 5/70 (7%)
Query: 17 ISIKVRGLDNAGKTTILKRFNGEPID-TISPTLG--FNIKTLEHKG--FKLNIWDVGGQK 71
I + + G GK+ +L RF+ + + T+G F I+T+E G KL IWD GQ+
Sbjct: 10 IKLLLIGDSGVGKSCLLLRFSEDSFTPSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQE 69
Query: 72 SLRSYWKNYF 81
R+ Y+
Sbjct: 70 RFRTITTAYY 79
>SPAC18G6.03 |ypt3||GTPase Ypt3|Schizosaccharomyces pombe|chr
1|||Manual
Length = 214
Score = 32.7 bits (71), Expect = 0.034
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 5/64 (7%)
Query: 23 GLDNAGKTTILKRFNGEPIDTISP-TLGFNIKT----LEHKGFKLNIWDVGGQKSLRSYW 77
G GK+ +L RF + S T+G T L++K K IWD GQ+ R+
Sbjct: 17 GDSGVGKSNLLMRFTRNEFNIESKSTIGVEFATRNIVLDNKKIKAQIWDTAGQERYRAIT 76
Query: 78 KNYF 81
Y+
Sbjct: 77 SAYY 80
>SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 216
Score = 31.9 bits (69), Expect = 0.059
Identities = 22/64 (34%), Positives = 27/64 (42%), Gaps = 5/64 (7%)
Query: 23 GLDNAGKTTILKR-FNGEPIDTISPTLGFNIKTLE-HKGFK---LNIWDVGGQKSLRSYW 77
G GKTT +KR GE TLG + L H F N+WD GQ+ L
Sbjct: 16 GDGGTGKTTFVKRHLTGEFEKKYIATLGVEVHPLHFHTNFGEICFNVWDTAGQEKLGGLR 75
Query: 78 KNYF 81
Y+
Sbjct: 76 DGYY 79
>SPAC6F6.15 |ypt5||GTPase Ypt5|Schizosaccharomyces pombe|chr
1|||Manual
Length = 211
Score = 31.5 bits (68), Expect = 0.078
Identities = 22/78 (28%), Positives = 39/78 (50%), Gaps = 6/78 (7%)
Query: 12 MVPVSISIKVRGLDNAGKTTILKRFNGEPIDTI-SPTLG--FNIKTL---EHKGFKLNIW 65
+V ++ + + G GK++++ RF + D T+G F +TL E+ KL IW
Sbjct: 10 VVTINQKLVLLGDSAVGKSSLVLRFVKDQFDDYRESTIGAAFLTQTLPIDENTSVKLEIW 69
Query: 66 DVGGQKSLRSYWKNYFES 83
D GQ+ +S Y+ +
Sbjct: 70 DTAGQERYKSLAPMYYRN 87
>SPAC1565.06c |spg1|sid3|GTPase Spg1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 198
Score = 30.7 bits (66), Expect = 0.14
Identities = 19/62 (30%), Positives = 36/62 (58%), Gaps = 5/62 (8%)
Query: 15 VSISIKVRGLDNAGKTTILKRF-NGEPIDTISPTLGFNI--KTLEHKGFKL--NIWDVGG 69
V+I + + G + GKT+++ + G + + TLG N KT+ + ++ +IWD+GG
Sbjct: 9 VTIKVGMIGDSSIGKTSLMVTYVQGSFDEESTQTLGVNFMEKTISIRNTEITFSIWDLGG 68
Query: 70 QK 71
Q+
Sbjct: 69 QR 70
>SPCC417.08 |tef3||translation elongation factor
eEF3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1047
Score = 27.1 bits (57), Expect = 1.7
Identities = 13/32 (40%), Positives = 20/32 (62%)
Query: 13 VPVSISIKVRGLDNAGKTTILKRFNGEPIDTI 44
V +S I V G + AGK+T++K GE + T+
Sbjct: 697 VSLSSRIAVIGPNGAGKSTLIKVLTGELLPTV 728
>SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr
1|||Manual
Length = 208
Score = 27.1 bits (57), Expect = 1.7
Identities = 17/72 (23%), Positives = 31/72 (43%), Gaps = 5/72 (6%)
Query: 9 MGTMVPVSISIKVRGLDNAGKTTILKRFNGEPIDT-ISPTLGFNIKT----LEHKGFKLN 63
M V + + + G GKT ++ +F + T+G + T ++ K L
Sbjct: 1 MSAQKRVFLKVVILGDSGVGKTCLMNQFVNQKFSREYKATIGADFLTKDVVVDDKLVTLQ 60
Query: 64 IWDVGGQKSLRS 75
+WD GQ+ +S
Sbjct: 61 LWDTAGQERFQS 72
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 26.6 bits (56), Expect = 2.2
Identities = 12/30 (40%), Positives = 20/30 (66%), Gaps = 2/30 (6%)
Query: 54 TLEHKGFKLNIWDVGGQKSLRSYWKNYFES 83
TL+H ++ ++DVGGQ++ R W FE+
Sbjct: 188 TLDH--LQIRMFDVGGQRTERRKWIYCFEN 215
>SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 205
Score = 26.6 bits (56), Expect = 2.2
Identities = 17/64 (26%), Positives = 30/64 (46%), Gaps = 5/64 (7%)
Query: 17 ISIKVRGLDNAGKTTILKRF-NGEPIDTISPTLGFNIKTLE----HKGFKLNIWDVGGQK 71
+ + + G GKT+I+ ++ N + T+G + T E K L +WD GQ+
Sbjct: 9 LKVIILGESGVGKTSIMNQYVNRKFSKDYKATIGADFLTKEVLVDDKVVTLQLWDTAGQE 68
Query: 72 SLRS 75
+S
Sbjct: 69 RFQS 72
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 26.2 bits (55), Expect = 2.9
Identities = 13/37 (35%), Positives = 19/37 (51%)
Query: 47 TLGFNIKTLEHKGFKLNIWDVGGQKSLRSYWKNYFES 83
T G + +T +DVGGQ+S R W + FE+
Sbjct: 221 TTGISEETFLLNRHHYRFFDVGGQRSERRKWIHCFEN 257
>SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 25.4 bits (53), Expect = 5.1
Identities = 11/40 (27%), Positives = 20/40 (50%)
Query: 17 ISIKVRGLDNAGKTTILKRFNGEPIDTISPTLGFNIKTLE 56
I++ + G NAGK++++ I +SP G +E
Sbjct: 240 INVAILGPSNAGKSSLINLLANRRISIVSPQSGTTRDAIE 279
>SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 419
Score = 25.4 bits (53), Expect = 5.1
Identities = 22/57 (38%), Positives = 29/57 (50%), Gaps = 11/57 (19%)
Query: 23 GLDNAGKTTIL------KRFNGE-PIDTISPTLGFNIKTL---EHKGFKLNIWDVGG 69
GL NAGK+T+L K GE TI P +G IKT +H F+ + D+ G
Sbjct: 243 GLPNAGKSTLLNCLTASKSKVGEYEFTTIYPKIG-TIKTTMPDDHSSFQYRLADIPG 298
>SPCC1223.13 |cbf12||CBF1/Su|Schizosaccharomyces pombe|chr
3|||Manual
Length = 963
Score = 25.4 bits (53), Expect = 5.1
Identities = 11/23 (47%), Positives = 14/23 (60%)
Query: 39 EPIDTISPTLGFNIKTLEHKGFK 61
EP +SP L F +TL H G+K
Sbjct: 935 EPTVELSPILLFQYETLFHSGYK 957
>SPCC825.01 |||ribosome biogenesis ATPase, Arb family
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 822
Score = 24.6 bits (51), Expect = 8.9
Identities = 17/72 (23%), Positives = 36/72 (50%), Gaps = 9/72 (12%)
Query: 5 IFFSMGTMVPVSISIKVRGLDNAGKTTILKRFNGEPIDTISPTLGFNIKTLEHKGFKLNI 64
IF + + + + + G + AGKTT++K ++ + P+ G + H G +L +
Sbjct: 609 IFSKLNFGLDLKSRVALVGPNGAGKTTLIKLI----LEKVQPSTG---SVVRHHGLRLAL 661
Query: 65 WD--VGGQKSLR 74
++ +G Q +R
Sbjct: 662 FNQHMGDQLDMR 673
>SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 537
Score = 24.6 bits (51), Expect = 8.9
Identities = 10/33 (30%), Positives = 19/33 (57%)
Query: 17 ISIKVRGLDNAGKTTILKRFNGEPIDTISPTLG 49
IS+ + G NAGK++I+ + + ++P G
Sbjct: 311 ISVGLIGFPNAGKSSIINTLRKKKVCNVAPIPG 343
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.322 0.138 0.434
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 653,060
Number of Sequences: 5004
Number of extensions: 20507
Number of successful extensions: 73
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 52
Number of HSP's gapped (non-prelim): 23
length of query: 184
length of database: 2,362,478
effective HSP length: 69
effective length of query: 115
effective length of database: 2,017,202
effective search space: 231978230
effective search space used: 231978230
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 51 (24.6 bits)
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