BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000772-TA|BGIBMGA000772-PA|IPR002018|Carboxylesterase, type B (567 letters) Database: bee 429 sequences; 140,377 total letters Searching.....................................................done Score E Sequences producing significant alignments: (bits) Value AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone este... 158 1e-40 AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein. 158 1e-40 AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 133 4e-33 AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 128 9e-32 AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein. 71 3e-14 AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein. 36 0.001 DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride... 23 8.9 DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 23 8.9 DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 23 8.9 >AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone esterase protein. Length = 567 Score = 158 bits (383), Expect = 1e-40 Identities = 143/550 (26%), Positives = 231/550 (42%), Gaps = 34/550 (6%) Query: 33 QTESGWVCGRSRRAEASTLYASFRGVPYAKQPVGELRFKELQPAEPWTDYLDATEEGPVC 92 +T G + G + + Y ++ G+PYA PVG+ RFK Q W L AT+ G C Sbjct: 26 KTPLGAIKGYYKISGNGKQYEAYEGIPYALPPVGKFRFKAPQKIPAWIGELSATKFGFPC 85 Query: 93 YQ-TDVLYGSLMKPHGMNEACIYANIHVPLYALPAAGETPTKPGLPILVFIHXXXXXXXX 151 Q T + K G E C+Y N++VP A TP++ LP++ +IH Sbjct: 86 LQYTQLPVNPRDKIEGA-EDCLYLNVYVP------ADRTPSQ-SLPVIFWIHGGAFQFGS 137 Query: 152 XXXXLYGPEYLVTRNVVVITFNYRLNFFGFFSLDTPKVPGNNGLRDMVTLLRWVKRNARA 211 G +YL+ +V+ +T NYRL GF S + VPGN GL+D LRWV N Sbjct: 138 GIPM--GAKYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEW 195 Query: 212 FGGNPDNVXXXXXXXXXXXXXXXXXXKATEGLVSRAILMSGAGXXXXXXXXXXXXXXINK 271 FGGNP + + GL I +SG K Sbjct: 196 FGGNPKRITLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSLEKA--K 253 Query: 272 LLFSILGVNSTNPDEIHEKLVAMPVEKLNE--ANRILIDQIGLTTFFPVVETPHPGITTI 329 + + +G + N E+ L P + E AN + T F PV E + ++ Sbjct: 254 QVGAFMGCPTRNVKEMIRCLRYRPARAIVETLANFMRFYYNPFTPFGPVTEKVNNDSNSL 313 Query: 330 --LDEDPNILVQQGRGKDIPLIIGFTNSE-CHMFQRRFEQIDIVSKINENPAILVPSNLL 386 +D P ++ G +D+P + G T+ E + + + + ++EN ++ P L Sbjct: 314 PFIDRTPIEIINSGDVQDVPWVTGVTSEEGLYPVAEFIAKPEALKLLDENWDLIAPYFLD 373 Query: 387 YSST--PETIALVSNQISQRYF-NGSVD---LEGFINMCTDSYYKYPALNLAEKRSTAGD 440 Y+ T E V+ I YF + +D L+ I++ +D ++ A ++ Sbjct: 374 YNYTIPKEKHVEVARLIRNYYFESNKIDETTLKHLIDVASDRFFITDGEKAARMQAKVNR 433 Query: 441 APVFLYQFSYDGYSVFKQAFHLHFN--GAGHADDLTYVLKVNSASGTSSSQKADDEMKYW 498 PV+ Y ++Y G + N G HADD V+ + T+++ D +M+ Sbjct: 434 QPVWFYYYTYKGAHSISEIMSGTSNKYGVCHADDAYMVVDTPFLASTTTTN--DIKMQKV 491 Query: 499 MTTFVTNFMRCSAPMCDETTAWPPVTPRE--LQYQDIITPNLCHQTSLTKEQLEMKNFFD 556 + F +F+ P + + WP + P E L Y I P S T ++F++ Sbjct: 492 LIDFWVSFVNNGVPNVN-SVQWPRLNPNEKSLHYLHIAGPGKIQMDSST--NFGREDFWN 548 Query: 557 KIHNGGESRL 566 I N E++L Sbjct: 549 SI-NFNENKL 557 >AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein. Length = 567 Score = 158 bits (383), Expect = 1e-40 Identities = 143/550 (26%), Positives = 231/550 (42%), Gaps = 34/550 (6%) Query: 33 QTESGWVCGRSRRAEASTLYASFRGVPYAKQPVGELRFKELQPAEPWTDYLDATEEGPVC 92 +T G + G + + Y ++ G+PYA PVG+ RFK Q W L AT+ G C Sbjct: 26 KTPLGAIKGYYKISGNGKQYEAYEGIPYALPPVGKFRFKAPQKIPAWIGELSATKFGFPC 85 Query: 93 YQ-TDVLYGSLMKPHGMNEACIYANIHVPLYALPAAGETPTKPGLPILVFIHXXXXXXXX 151 Q T + K G E C+Y N++VP A TP++ LP++ +IH Sbjct: 86 LQYTQLPVNPRDKIEGA-EDCLYLNVYVP------ADRTPSQ-SLPVIFWIHGGAFQFGS 137 Query: 152 XXXXLYGPEYLVTRNVVVITFNYRLNFFGFFSLDTPKVPGNNGLRDMVTLLRWVKRNARA 211 G +YL+ +V+ +T NYRL GF S + VPGN GL+D LRWV N Sbjct: 138 GIPM--GAKYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEW 195 Query: 212 FGGNPDNVXXXXXXXXXXXXXXXXXXKATEGLVSRAILMSGAGXXXXXXXXXXXXXXINK 271 FGGNP + + GL I +SG K Sbjct: 196 FGGNPKRITLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSLEKA--K 253 Query: 272 LLFSILGVNSTNPDEIHEKLVAMPVEKLNE--ANRILIDQIGLTTFFPVVETPHPGITTI 329 + + +G + N E+ L P + E AN + T F PV E + ++ Sbjct: 254 QVGAFMGCPTRNVKEMIRCLRYRPARAIVETLANFMRFYYNPFTPFGPVTEKVNNDSNSL 313 Query: 330 --LDEDPNILVQQGRGKDIPLIIGFTNSE-CHMFQRRFEQIDIVSKINENPAILVPSNLL 386 +D P ++ G +D+P + G T+ E + + + + ++EN ++ P L Sbjct: 314 PFIDRTPIEIINSGDVQDVPWVTGVTSEEGLYPVAEFIAKPEALKLLDENWDLIAPYFLD 373 Query: 387 YSST--PETIALVSNQISQRYF-NGSVD---LEGFINMCTDSYYKYPALNLAEKRSTAGD 440 Y+ T E V+ I YF + +D L+ I++ +D ++ A ++ Sbjct: 374 YNYTIPKEKHVEVARLIRNYYFESNKIDETTLKHLIDVASDRFFITDGEKAARMQAKVNR 433 Query: 441 APVFLYQFSYDGYSVFKQAFHLHFN--GAGHADDLTYVLKVNSASGTSSSQKADDEMKYW 498 PV+ Y ++Y G + N G HADD V+ + T+++ D +M+ Sbjct: 434 QPVWFYYYTYKGAHSISEIMSGTSNKYGVCHADDAYMVVDTPFLASTTTTN--DIKMQKV 491 Query: 499 MTTFVTNFMRCSAPMCDETTAWPPVTPRE--LQYQDIITPNLCHQTSLTKEQLEMKNFFD 556 + F +F+ P + + WP + P E L Y I P S T ++F++ Sbjct: 492 LIDFWVSFVNNGVPNVN-SVQWPRLNPNEKSLHYLHIAGPGKIQMDSST--NFGREDFWN 548 Query: 557 KIHNGGESRL 566 I N E++L Sbjct: 549 SI-NFNENKL 557 >AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase protein. Length = 628 Score = 133 bits (321), Expect = 4e-33 Identities = 135/533 (25%), Positives = 228/533 (42%), Gaps = 47/533 (8%) Query: 30 VVAQTESGWVCGRSRRAEASTLYASFRGVPYAKQPVGELRFKELQPAEPWTDYLDATEEG 89 +V +T SG V G R ++ F G+P+AK P+G LRF++ P EPW L+AT Sbjct: 38 LVVETTSGLVRGFPRTVLDKEVHV-FYGIPFAKPPIGPLRFRKPLPIEPWHGVLNATVLP 96 Query: 90 PVCYQTDVLY------GSLMKPH-GMNEACIYANIHVPL-YAL-------PAAGETPTKP 134 CYQ Y + P+ ++E C+Y NI VP Y L P P Sbjct: 97 NSCYQERYEYFPGFPGEEMWNPNTNISEDCLYLNIWVPQKYRLRHKGDGSPGGNGGPRNG 156 Query: 135 GLPILVFIHXXXXXXXXXXXXLYGPEYLV-TRNVVVITFNYRLNFFGFFSLD-----TPK 188 LP+LV+I+ +Y + + T NV++ + YR+ FGF L+ + + Sbjct: 157 LLPLLVWIYGGGFMSGTATLDVYNADIMAATSNVIIASMQYRVGAFGFLYLNKHFTNSEE 216 Query: 189 VPGNNGLRDMVTLLRWVKRNARAFGGNPDNVXXXXXXXXXXXXXXXXXXKATEGLVSRAI 248 PGN GL D LRW++ NA AFGG+P+ + T GLV R I Sbjct: 217 APGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSVSLHLISPVTRGLVRRGI 276 Query: 249 LMSGAGXXXXXXXXXXXXXXINKLLFSILGVNSTNPDEIHEKLVAMPVEKLNEANRILID 308 L SG + +L G NST +E +++A + +A I + Sbjct: 277 LQSGTLNAPWSYMSGEKANEVATILVDDCGCNSTMLNENPARVMA--CMRSVDAKTISVQ 334 Query: 309 QIGLTTFFPVVETPH-PGITTI-LDEDPNILVQQGRGKDIPLIIGFTNSE-----CHMFQ 361 Q +++ ++ P P I I L + P L+++ KD ++IG +E + F Sbjct: 335 Q--WNSYWGILGFPSAPTIDGIFLPKHPLDLLREADFKDTEILIGNNENEGTYFILYDFN 392 Query: 362 RRFEQIDIVSKINENPAILVPSNLLYSSTPETIALVSNQIS--QRYFNGSVDLEGFINMC 419 FE+ D S + + + +N+ + + ++ Q + + +NG + + ++ Sbjct: 393 DIFEK-DQASFLERERFLGIINNIFKNMSQIEREAITFQYTDWEEVYNGYIYQKMVADVV 451 Query: 420 TDSYYKYPALNLAEKRSTAGDAPVFLYQFSYDGYSVFKQAFHL--HFNGAGHADDLTYVL 477 D ++ P+++ A+ + G V+ Y F+ + + +L + G H D++ YV Sbjct: 452 GDYFFICPSIHFAQLFADRG-MKVYYYFFTQ------RTSTNLWGEWMGVLHGDEVEYVF 504 Query: 478 KVNSASGTSSSQKADDEMKYWMTTFVTNFMRCSAPMCDETTAWPPVTPRELQY 530 S K D + M + + F P E + WP + E +Y Sbjct: 505 GHPLNKSLKYSDKERD-LSLRMILYFSEFAYLGKP-TKEDSEWPSYSRDEPKY 555 >AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase protein. Length = 492 Score = 128 bits (310), Expect = 9e-32 Identities = 118/450 (26%), Positives = 199/450 (44%), Gaps = 37/450 (8%) Query: 30 VVAQTESGWVCGRSRRAEASTLYASFRGVPYAKQPVGELRFKELQPAEPWTDYLDATEEG 89 +V +T SG V G R ++ F G+P+AK P+G LRF++ P EPW L+AT Sbjct: 38 LVVETTSGLVRGFPRTVLDKEVHV-FYGIPFAKPPIGPLRFRKPLPIEPWHGVLNATVLP 96 Query: 90 PVCYQTDVLY------GSLMKPH-GMNEACIYANIHVPL-YAL-------PAAGETPTKP 134 CYQ Y + P+ ++E C+Y NI VP Y L P P Sbjct: 97 NSCYQERYEYFPGFPGEEMWNPNTNISEDCLYLNIWVPQKYRLRHKGDGSPGGNGGPRNG 156 Query: 135 GLPILVFIHXXXXXXXXXXXXLYGPEYLV-TRNVVVITFNYRLNFFGFFSLD-----TPK 188 LP+LV+I+ +Y + + T NV++ + YR+ FGF L+ + + Sbjct: 157 LLPLLVWIYGGGFMSGTATLDVYNADIMAATSNVIIASMQYRVGAFGFLYLNKHFTNSEE 216 Query: 189 VPGNNGLRDMVTLLRWVKRNARAFGGNPDNVXXXXXXXXXXXXXXXXXXKATEGLVSRAI 248 PGN GL D LRW++ NA AFGG+P+ + T GLV R I Sbjct: 217 APGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSVSLHLISPVTRGLVRRGI 276 Query: 249 LMSGAGXXXXXXXXXXXXXXINKLLFSILGVNSTNPDEIHEKLVAMPVEKLNEANRILID 308 L SG + +L G NST +E +++A + +A I + Sbjct: 277 LQSGTLNAPWSYMSGEKANEVATILVDDCGCNSTMLNENPARVMA--CMRSVDAKTISVQ 334 Query: 309 QIGLTTFFPVVETPH-PGITTI-LDEDPNILVQQGRGKDIPLIIGFTNSE-----CHMFQ 361 Q +++ ++ P P I I L + P L+++ KD ++IG +E + F Sbjct: 335 Q--WNSYWGILGFPSAPTIDGIFLPKHPLDLLREADFKDTEILIGNNENEGTYFILYDFN 392 Query: 362 RRFEQIDIVSKINENPAILVPSNLLYSSTPETIALVSNQIS--QRYFNGSVDLEGFINMC 419 FE+ D S + + + +N+ + + ++ Q + + +NG + + ++ Sbjct: 393 DIFEK-DQASFLERERFLGIINNIFKNMSQIEREAITFQYTDWEEVYNGYIYQKMVADVV 451 Query: 420 TDSYYKYPALNLAEKRSTAGDAPVFLYQFS 449 D ++ P+++ A+ + G V+ Y F+ Sbjct: 452 GDYFFICPSIHFAQLFADRG-MKVYYYFFT 480 >AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein. Length = 169 Score = 70.9 bits (166), Expect = 3e-14 Identities = 34/95 (35%), Positives = 44/95 (46%) Query: 158 GPEYLVTRNVVVITFNYRLNFFGFFSLDTPKVPGNNGLRDMVTLLRWVKRNARAFGGNPD 217 G +YL+ +V+ +T NYRL GF S + VPGN GL+D LRWV N FGGNP Sbjct: 13 GAKYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGGNPK 72 Query: 218 NVXXXXXXXXXXXXXXXXXXKATEGLVSRAILMSG 252 + + GL I +SG Sbjct: 73 RITLIGLSAGGASVHYHYLSPLSAGLFQGGISISG 107 >AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein. Length = 62 Score = 35.5 bits (78), Expect = 0.001 Identities = 13/44 (29%), Positives = 24/44 (54%) Query: 137 PILVFIHXXXXXXXXXXXXLYGPEYLVTRNVVVITFNYRLNFFG 180 P++ ++H P+YL+ ++VVV++ NYR+ FG Sbjct: 19 PVMFYVHEGAFISGTSSFHEMRPDYLLPKDVVVVSSNYRVGAFG 62 >DQ667192-1|ABG75744.1| 489|Apis mellifera pH-sensitive chloride channel variant 4 protein. Length = 489 Score = 22.6 bits (46), Expect = 8.9 Identities = 8/15 (53%), Positives = 12/15 (80%) Query: 352 FTNSECHMFQRRFEQ 366 F +S+C + QRRFE+ Sbjct: 269 FGDSKCSLCQRRFEE 283 >DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 22.6 bits (46), Expect = 8.9 Identities = 13/48 (27%), Positives = 21/48 (43%), Gaps = 1/48 (2%) Query: 516 ETTAWPPVTPRELQ-YQDIITPNLCHQTSLTKEQLEMKNFFDKIHNGG 562 E T + P P + ++ +TP H + +KE L + D GG Sbjct: 385 EKTCYYPYHPSTQEDSEEHLTPKRFHSRAASKEDLSPSSLADGARFGG 432 >DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 22.6 bits (46), Expect = 8.9 Identities = 13/48 (27%), Positives = 21/48 (43%), Gaps = 1/48 (2%) Query: 516 ETTAWPPVTPRELQ-YQDIITPNLCHQTSLTKEQLEMKNFFDKIHNGG 562 E T + P P + ++ +TP H + +KE L + D GG Sbjct: 385 EKTCYYPYHPSTQEDSEEHLTPKRFHSRAASKEDLSPSSLADGARFGG 432 Database: bee Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 140,377 Number of sequences in database: 429 Lambda K H 0.320 0.137 0.417 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 150,435 Number of Sequences: 429 Number of extensions: 5970 Number of successful extensions: 27 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 6 Number of HSP's gapped (non-prelim): 9 length of query: 567 length of database: 140,377 effective HSP length: 61 effective length of query: 506 effective length of database: 114,208 effective search space: 57789248 effective search space used: 57789248 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 46 (22.6 bits)
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