BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000767-TA|BGIBMGA000767-PA|IPR003191|Guanylate-binding protein (564 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g38840.1 68415.m04772 guanylate-binding family protein simila... 52 1e-06 At5g46070.1 68418.m05665 guanylate-binding family protein contai... 42 0.001 At5g44270.1 68418.m05418 hypothetical protein 31 2.7 At4g13460.1 68417.m02102 SET domain-containing protein (SUVH9) i... 31 2.7 At1g51965.1 68414.m05859 pentatricopeptide (PPR) repeat-containi... 30 3.6 At4g20400.1 68417.m02978 transcription factor jumonji (jmj) fami... 30 4.7 At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative i... 30 4.7 At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 29 6.2 At5g57035.1 68418.m07119 protein kinase family protein contains ... 29 6.2 At1g05320.1 68414.m00539 myosin-related similar to non-muscle my... 29 8.2 >At2g38840.1 68415.m04772 guanylate-binding family protein similar to SP|Q01514 Interferon-induced guanylate-binding protein 1 (Guanine nucleotide-binding protein 1) (Interferon-gamma inducible protein MAG-1) {Mus musculus}; contains Pfam profile PF02263: Guanylate-binding protein, N-terminal domain Length = 679 Score = 51.6 bits (118), Expect = 1e-06 Identities = 48/154 (31%), Positives = 74/154 (48%), Gaps = 29/154 (18%) Query: 21 VVDTGPDHT-FTLDEEALKSLLLKEDVRDRAVVVISVAGAFRKGKSFLLDFFLRYMHYKY 79 +V+ P HT L E L+++ + ++V G +R GKSFLL+ L Y Sbjct: 33 IVEPAPGHTKLQLSREGLEAI----SRITTPISAVAVIGPYRSGKSFLLNQLLSLSCY-- 86 Query: 80 XXXXXXXXXXXXXXDPLQGFSWRGGSERDTTGILMWSEIFKATLDDGEKVAIILLDTQGA 139 +GF + T GI +W + +D G K ++I LDT+G Sbjct: 87 -----------------EGFGVGHMRDTKTKGIWVWGTPLELEID-GVKTSVIYLDTEG- 127 Query: 140 FDS--ESTVRDCATVFALSTMLSSVQIYNLSQNI 171 F+S +S V D +FAL+T++SSV IYNL + + Sbjct: 128 FESVGKSNVYD-DRIFALATVMSSVLIYNLPETV 160 Score = 35.9 bits (79), Expect = 0.071 Identities = 34/173 (19%), Positives = 73/173 (42%), Gaps = 14/173 (8%) Query: 238 ELQSLRKHITACFSELACFLMPHPGLKVATNPQFDGRLADIESEFKRSLQQFVPMLLAPE 297 ++ +R + F +P P L D + D++S + Q ++ + Sbjct: 307 QVNQIRDSLAIMGDNSTAFSLPQPHLM--RTKLCDLKDEDLDSTYVARRDQLKKLVASI- 363 Query: 298 NLVPKLINGQKVKAKDLVQYFKSYMNIYKGNELPEPKSMLVATAEANNLTAVAEAKEVYT 357 + PK++ G+ + K+ + + + ++ E+P S++ E N V ++Y Sbjct: 364 -IRPKIVQGKTLNGKEFISFLEQILDALNKGEIPSTGSLV----EVFNKDIVERCVKLYN 418 Query: 358 RLMEEVCGGGRPYLHADLLLTEHQRVRDKAMYAFNAKRKMGGRDFSQAYFDQL 410 M V R + + L + H+ ++A+ AF+A+ GR ++ DQL Sbjct: 419 EKMVRV----RLPMSEESLQSAHEMAHNEAIKAFDAQH--FGRQHAKKSVDQL 465 >At5g46070.1 68418.m05665 guanylate-binding family protein contains Pfam domains PF02263: Guanylate-binding protein, N-terminal domain and PF02841: Guanylate-binding protein, C-terminal domain Length = 1060 Score = 41.5 bits (93), Expect = 0.001 Identities = 85/381 (22%), Positives = 137/381 (35%), Gaps = 47/381 (12%) Query: 16 PHGVQVVDTGPDHTFTLDEEALKSLLLKEDVRDRAVVVISVAGAFRKGKSFLLDFFLRYM 75 P +++V F +D EA+ +L L ++ + V+SV G R+GKSF+L+ L Sbjct: 39 PRPIRLVYCDEKGKFRMDPEAVATLQLVKE----PIGVVSVCGRARQGKSFILNQLLGRS 94 Query: 76 HYKYXXXXXXXXXXXXXXDPLQGFSWRGGSERDTTGILMWSEIFKATLDDGEKVAIILLD 135 + GF + T G+ +WS K T DG + ++LLD Sbjct: 95 N---------------------GFQVASTHKPCTKGLWLWSSPIKRTALDGTEYNLLLLD 133 Query: 136 TQG--AFDSESTVRDCATVFALSTMLSSVQIYNLSQNIQEDDLQHLQLFTEYGRLALEDG 193 ++G A+D D A++ LS + + + + L F+ L D Sbjct: 134 SEGIDAYDQTMGGIDEASLDRLSLVTQMTKHIRVKASGGTSSRSELGQFSPIFVWLLRD- 192 Query: 194 GRTPFQRLQFLVRDWSFPYEAPYGADGGMQILSRRLKVSDKQHPELQSLRKHITACFSEL 253 LV D Y ++I R ++ S +R I A F + Sbjct: 193 ------FYLDLVEDNRKISPRDY-----LEIALRPVQGSGGDIGAKNEIRDSIRALFPDR 241 Query: 254 ACFLMPHPGLKVATNPQFDG-RLADIESEFKRSLQQFVPMLLAPENLVPKLINGQKVKAK 312 CF + P + D L + EF L F + E PK + G + Sbjct: 242 ECFTLVRPLNNEKDLQRLDQISLEKLRPEFGAGLDAFTKFVF--EKTRPKQLGGTVMTGP 299 Query: 313 DLVQYFKSYMNIYKGNELPEPKSMLVATAEANNLTAVAEAKEVYTRLMEEVCGGGRPYLH 372 LV +SY++ +P S + E A E Y ++ P Sbjct: 300 ILVGITQSYLDALNNGAVPTITSSWQSVEETECRRAYDSGVEAYMAAFDQ---SKAP--E 354 Query: 373 ADLLLTEHQRVRDKAMYAFNA 393 L EH+ KA+ FN+ Sbjct: 355 EGALREEHEEAVRKALAIFNS 375 >At5g44270.1 68418.m05418 hypothetical protein Length = 309 Score = 30.7 bits (66), Expect = 2.7 Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 3/69 (4%) Query: 224 ILSRRLKVSDKQHPELQSLR-KH-ITACFSE-LACFLMPHPGLKVATNPQFDGRLADIES 280 + S+R++ + E++ L+ KH + CF++ LA MP P P+F D + Sbjct: 119 VTSQRVRPKQQSCTEMRKLKTKHALPLCFTKILAAPAMPTPQRSTPHPPEFQVNHTDQTT 178 Query: 281 EFKRSLQQF 289 FKRS ++ Sbjct: 179 RFKRSYPKY 187 >At4g13460.1 68417.m02102 SET domain-containing protein (SUVH9) identical to SUVH9 [Arabidopsis thaliana] GI:13517759; contains Pfam profiles PF00856: SET domain, PF05033: Pre-SET motif, PF02182: YDG/SRA domain; identical to cDNA SUVH9 (SUVH9) GI:13517758 Length = 650 Score = 30.7 bits (66), Expect = 2.7 Identities = 12/42 (28%), Positives = 22/42 (52%) Query: 6 TEKPAPAPRGPHGVQVVDTGPDHTFTLDEEALKSLLLKEDVR 47 T++P P PR V++ D GP+ E K+ ++ + +R Sbjct: 118 TQRPRPQPRSSELVRITDVGPESERQFREHVRKTRMIYDSLR 159 >At1g51965.1 68414.m05859 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 650 Score = 30.3 bits (65), Expect = 3.6 Identities = 28/102 (27%), Positives = 46/102 (45%), Gaps = 10/102 (9%) Query: 306 GQKVKAKDLVQYFKSYMNIYKGNELPEPKSMLVATAEANNLTA------VAEAKEVYTRL 359 GQ V+ +V+ K YM + L S L +EA+ L V ++ Y + Sbjct: 354 GQLVRLDGVVEISKRYMTQGIYSYLVRTLSKLGHVSEAHRLFCDMWSFPVKGERDSYMSM 413 Query: 360 MEEVCGGGRPYLHADLLLTEHQR--VRDKAMY--AFNAKRKM 397 +E +CG G+ ++L H++ V D MY F+A K+ Sbjct: 414 LESLCGAGKTIEAIEMLSKIHEKGVVTDTMMYNTVFSALGKL 455 >At4g20400.1 68417.m02978 transcription factor jumonji (jmj) family protein / zinc finger (C5HC2 type) family protein contains Pfam domains, PF02375: jmjN domain, PF02373: jmjC domain and PF02928: C5HC2 zinc finger Length = 954 Score = 29.9 bits (64), Expect = 4.7 Identities = 11/29 (37%), Positives = 19/29 (65%) Query: 260 HPGLKVATNPQFDGRLADIESEFKRSLQQ 288 HPG K + N +F ++ D+E E+ R ++Q Sbjct: 199 HPGSKASENKKFKPKVKDLEGEYWRIVEQ 227 >At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative identical to RNA polymerase subunit [Arabidopsis thaliana] GI:514322; contains Pfam profile PF01000: RNA polymerase Rpb3/RpoA insert domain Length = 302 Score = 29.9 bits (64), Expect = 4.7 Identities = 12/34 (35%), Positives = 22/34 (64%), Gaps = 1/34 (2%) Query: 234 KQHPELQSLRKHITACFSELA-CFLMPHPGLKVA 266 + HPE++S+ +++ E A CF+ PHP + V+ Sbjct: 269 RMHPEVRSIYINLSYALWETALCFVFPHPNIYVS 302 >At5g67270.1 68418.m08480 microtubule-associated EB1 family protein similar to SP|Q9UPY8 Microtubule-associated protein RP/EB family member 3 (Protein EB3) {Homo sapiens}; contains Pfam profiles PF00307: Calponin homology (CH) domain, PF03271: EB1 protein Length = 329 Score = 29.5 bits (63), Expect = 6.2 Identities = 22/92 (23%), Positives = 38/92 (41%), Gaps = 3/92 (3%) Query: 241 SLRKHITACFSELACFLMP--HPGLKVATNPQFDGRLA-DIESEFKRSLQQFVPMLLAPE 297 +L K AC + C LM HPG FD + ++ +K F + + Sbjct: 30 NLSKVEEACSGAVHCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITKH 89 Query: 298 NLVPKLINGQKVKAKDLVQYFKSYMNIYKGNE 329 V KL+ G+ + + +Q+ K Y + G + Sbjct: 90 IEVSKLVKGRPLDNLEFMQWMKKYCDSVNGGQ 121 >At5g57035.1 68418.m07119 protein kinase family protein contains Pfam profile: PF00069 Eukaryotic protein kinase domain Length = 786 Score = 29.5 bits (63), Expect = 6.2 Identities = 47/202 (23%), Positives = 81/202 (40%), Gaps = 15/202 (7%) Query: 206 RDWSFPYEAPYG-ADGGMQILSRRLKVSDKQHPELQSLRKHITACFSELACFLMPHPGLK 264 R S P+ P Q S + + D +H +S+ +H T S++ + P P LK Sbjct: 230 RPGSLPHSHPTSRVYSDAQSSSTDIVLVDDEH--CRSILRHSTVSTSKIQ--MDPRPHLK 285 Query: 265 VATNPQFDGRLADIESEFKRSLQQFVPMLL-APENLVPKLINGQKVKAKDLVQYFKSYMN 323 + G A++E + ++ +Q + M A E LV K Q + ++ + K Sbjct: 286 TPKS----GVRAEVE-QLRKEVQTTLSMYKQACEELVHKQTQVQSLSSECI----KETER 336 Query: 324 IYKGNELPEPKSMLVATAEANNLTAVAEAKEVYTRLMEEVCGGGRPYLHADLLLTEHQRV 383 + E E + A + +L AV E +E + L +E C L A E Q+V Sbjct: 337 VITALEKEEMRRKAAAEEKEKHLKAVKEVEEAKSMLAKEFCERQLAELDALKQSIEKQKV 396 Query: 384 RDKAMYAFNAKRKMGGRDFSQA 405 ++ RK + + A Sbjct: 397 IEQLFLRDGRYRKYTKEEIAAA 418 >At1g05320.1 68414.m00539 myosin-related similar to non-muscle myosin II heavy chain (GI:19879404) [Loligo pealei]; ESTs gb|AA042402,gb|ATTS1380 come from this gene Length = 828 Score = 29.1 bits (62), Expect = 8.2 Identities = 14/50 (28%), Positives = 27/50 (54%) Query: 275 LADIESEFKRSLQQFVPMLLAPENLVPKLINGQKVKAKDLVQYFKSYMNI 324 LAD ++ K +Q+ L + + + +N + +AKDL+ KS+ N+ Sbjct: 392 LADTNNQLKIKIQELEGYLDSEKETAIEKLNQKDTEAKDLITKLKSHENV 441 Database: arabidopsis Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.321 0.136 0.405 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,850,056 Number of Sequences: 28952 Number of extensions: 456037 Number of successful extensions: 968 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 961 Number of HSP's gapped (non-prelim): 13 length of query: 564 length of database: 12,070,560 effective HSP length: 85 effective length of query: 479 effective length of database: 9,609,640 effective search space: 4603017560 effective search space used: 4603017560 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 62 (29.1 bits)
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