Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000767-TA|BGIBMGA000767-PA|IPR003191|Guanylate-binding
protein
(564 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g38840.1 68415.m04772 guanylate-binding family protein simila... 52 1e-06
At5g46070.1 68418.m05665 guanylate-binding family protein contai... 42 0.001
At5g44270.1 68418.m05418 hypothetical protein 31 2.7
At4g13460.1 68417.m02102 SET domain-containing protein (SUVH9) i... 31 2.7
At1g51965.1 68414.m05859 pentatricopeptide (PPR) repeat-containi... 30 3.6
At4g20400.1 68417.m02978 transcription factor jumonji (jmj) fami... 30 4.7
At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative i... 30 4.7
At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 29 6.2
At5g57035.1 68418.m07119 protein kinase family protein contains ... 29 6.2
At1g05320.1 68414.m00539 myosin-related similar to non-muscle my... 29 8.2
>At2g38840.1 68415.m04772 guanylate-binding family protein similar
to SP|Q01514 Interferon-induced guanylate-binding
protein 1 (Guanine nucleotide-binding protein 1)
(Interferon-gamma inducible protein MAG-1) {Mus
musculus}; contains Pfam profile PF02263:
Guanylate-binding protein, N-terminal domain
Length = 679
Score = 51.6 bits (118), Expect = 1e-06
Identities = 48/154 (31%), Positives = 74/154 (48%), Gaps = 29/154 (18%)
Query: 21 VVDTGPDHT-FTLDEEALKSLLLKEDVRDRAVVVISVAGAFRKGKSFLLDFFLRYMHYKY 79
+V+ P HT L E L+++ + ++V G +R GKSFLL+ L Y
Sbjct: 33 IVEPAPGHTKLQLSREGLEAI----SRITTPISAVAVIGPYRSGKSFLLNQLLSLSCY-- 86
Query: 80 XXXXXXXXXXXXXXDPLQGFSWRGGSERDTTGILMWSEIFKATLDDGEKVAIILLDTQGA 139
+GF + T GI +W + +D G K ++I LDT+G
Sbjct: 87 -----------------EGFGVGHMRDTKTKGIWVWGTPLELEID-GVKTSVIYLDTEG- 127
Query: 140 FDS--ESTVRDCATVFALSTMLSSVQIYNLSQNI 171
F+S +S V D +FAL+T++SSV IYNL + +
Sbjct: 128 FESVGKSNVYD-DRIFALATVMSSVLIYNLPETV 160
Score = 35.9 bits (79), Expect = 0.071
Identities = 34/173 (19%), Positives = 73/173 (42%), Gaps = 14/173 (8%)
Query: 238 ELQSLRKHITACFSELACFLMPHPGLKVATNPQFDGRLADIESEFKRSLQQFVPMLLAPE 297
++ +R + F +P P L D + D++S + Q ++ +
Sbjct: 307 QVNQIRDSLAIMGDNSTAFSLPQPHLM--RTKLCDLKDEDLDSTYVARRDQLKKLVASI- 363
Query: 298 NLVPKLINGQKVKAKDLVQYFKSYMNIYKGNELPEPKSMLVATAEANNLTAVAEAKEVYT 357
+ PK++ G+ + K+ + + + ++ E+P S++ E N V ++Y
Sbjct: 364 -IRPKIVQGKTLNGKEFISFLEQILDALNKGEIPSTGSLV----EVFNKDIVERCVKLYN 418
Query: 358 RLMEEVCGGGRPYLHADLLLTEHQRVRDKAMYAFNAKRKMGGRDFSQAYFDQL 410
M V R + + L + H+ ++A+ AF+A+ GR ++ DQL
Sbjct: 419 EKMVRV----RLPMSEESLQSAHEMAHNEAIKAFDAQH--FGRQHAKKSVDQL 465
>At5g46070.1 68418.m05665 guanylate-binding family protein contains
Pfam domains PF02263: Guanylate-binding protein,
N-terminal domain and PF02841: Guanylate-binding
protein, C-terminal domain
Length = 1060
Score = 41.5 bits (93), Expect = 0.001
Identities = 85/381 (22%), Positives = 137/381 (35%), Gaps = 47/381 (12%)
Query: 16 PHGVQVVDTGPDHTFTLDEEALKSLLLKEDVRDRAVVVISVAGAFRKGKSFLLDFFLRYM 75
P +++V F +D EA+ +L L ++ + V+SV G R+GKSF+L+ L
Sbjct: 39 PRPIRLVYCDEKGKFRMDPEAVATLQLVKE----PIGVVSVCGRARQGKSFILNQLLGRS 94
Query: 76 HYKYXXXXXXXXXXXXXXDPLQGFSWRGGSERDTTGILMWSEIFKATLDDGEKVAIILLD 135
+ GF + T G+ +WS K T DG + ++LLD
Sbjct: 95 N---------------------GFQVASTHKPCTKGLWLWSSPIKRTALDGTEYNLLLLD 133
Query: 136 TQG--AFDSESTVRDCATVFALSTMLSSVQIYNLSQNIQEDDLQHLQLFTEYGRLALEDG 193
++G A+D D A++ LS + + + + L F+ L D
Sbjct: 134 SEGIDAYDQTMGGIDEASLDRLSLVTQMTKHIRVKASGGTSSRSELGQFSPIFVWLLRD- 192
Query: 194 GRTPFQRLQFLVRDWSFPYEAPYGADGGMQILSRRLKVSDKQHPELQSLRKHITACFSEL 253
LV D Y ++I R ++ S +R I A F +
Sbjct: 193 ------FYLDLVEDNRKISPRDY-----LEIALRPVQGSGGDIGAKNEIRDSIRALFPDR 241
Query: 254 ACFLMPHPGLKVATNPQFDG-RLADIESEFKRSLQQFVPMLLAPENLVPKLINGQKVKAK 312
CF + P + D L + EF L F + E PK + G +
Sbjct: 242 ECFTLVRPLNNEKDLQRLDQISLEKLRPEFGAGLDAFTKFVF--EKTRPKQLGGTVMTGP 299
Query: 313 DLVQYFKSYMNIYKGNELPEPKSMLVATAEANNLTAVAEAKEVYTRLMEEVCGGGRPYLH 372
LV +SY++ +P S + E A E Y ++ P
Sbjct: 300 ILVGITQSYLDALNNGAVPTITSSWQSVEETECRRAYDSGVEAYMAAFDQ---SKAP--E 354
Query: 373 ADLLLTEHQRVRDKAMYAFNA 393
L EH+ KA+ FN+
Sbjct: 355 EGALREEHEEAVRKALAIFNS 375
>At5g44270.1 68418.m05418 hypothetical protein
Length = 309
Score = 30.7 bits (66), Expect = 2.7
Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 3/69 (4%)
Query: 224 ILSRRLKVSDKQHPELQSLR-KH-ITACFSE-LACFLMPHPGLKVATNPQFDGRLADIES 280
+ S+R++ + E++ L+ KH + CF++ LA MP P P+F D +
Sbjct: 119 VTSQRVRPKQQSCTEMRKLKTKHALPLCFTKILAAPAMPTPQRSTPHPPEFQVNHTDQTT 178
Query: 281 EFKRSLQQF 289
FKRS ++
Sbjct: 179 RFKRSYPKY 187
>At4g13460.1 68417.m02102 SET domain-containing protein (SUVH9)
identical to SUVH9 [Arabidopsis thaliana] GI:13517759;
contains Pfam profiles PF00856: SET domain, PF05033:
Pre-SET motif, PF02182: YDG/SRA domain; identical to
cDNA SUVH9 (SUVH9) GI:13517758
Length = 650
Score = 30.7 bits (66), Expect = 2.7
Identities = 12/42 (28%), Positives = 22/42 (52%)
Query: 6 TEKPAPAPRGPHGVQVVDTGPDHTFTLDEEALKSLLLKEDVR 47
T++P P PR V++ D GP+ E K+ ++ + +R
Sbjct: 118 TQRPRPQPRSSELVRITDVGPESERQFREHVRKTRMIYDSLR 159
>At1g51965.1 68414.m05859 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 650
Score = 30.3 bits (65), Expect = 3.6
Identities = 28/102 (27%), Positives = 46/102 (45%), Gaps = 10/102 (9%)
Query: 306 GQKVKAKDLVQYFKSYMNIYKGNELPEPKSMLVATAEANNLTA------VAEAKEVYTRL 359
GQ V+ +V+ K YM + L S L +EA+ L V ++ Y +
Sbjct: 354 GQLVRLDGVVEISKRYMTQGIYSYLVRTLSKLGHVSEAHRLFCDMWSFPVKGERDSYMSM 413
Query: 360 MEEVCGGGRPYLHADLLLTEHQR--VRDKAMY--AFNAKRKM 397
+E +CG G+ ++L H++ V D MY F+A K+
Sbjct: 414 LESLCGAGKTIEAIEMLSKIHEKGVVTDTMMYNTVFSALGKL 455
>At4g20400.1 68417.m02978 transcription factor jumonji (jmj) family
protein / zinc finger (C5HC2 type) family protein
contains Pfam domains, PF02375: jmjN domain, PF02373:
jmjC domain and PF02928: C5HC2 zinc finger
Length = 954
Score = 29.9 bits (64), Expect = 4.7
Identities = 11/29 (37%), Positives = 19/29 (65%)
Query: 260 HPGLKVATNPQFDGRLADIESEFKRSLQQ 288
HPG K + N +F ++ D+E E+ R ++Q
Sbjct: 199 HPGSKASENKKFKPKVKDLEGEYWRIVEQ 227
>At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative
identical to RNA polymerase subunit [Arabidopsis
thaliana] GI:514322; contains Pfam profile PF01000: RNA
polymerase Rpb3/RpoA insert domain
Length = 302
Score = 29.9 bits (64), Expect = 4.7
Identities = 12/34 (35%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Query: 234 KQHPELQSLRKHITACFSELA-CFLMPHPGLKVA 266
+ HPE++S+ +++ E A CF+ PHP + V+
Sbjct: 269 RMHPEVRSIYINLSYALWETALCFVFPHPNIYVS 302
>At5g67270.1 68418.m08480 microtubule-associated EB1 family protein
similar to SP|Q9UPY8 Microtubule-associated protein
RP/EB family member 3 (Protein EB3) {Homo sapiens};
contains Pfam profiles PF00307: Calponin homology (CH)
domain, PF03271: EB1 protein
Length = 329
Score = 29.5 bits (63), Expect = 6.2
Identities = 22/92 (23%), Positives = 38/92 (41%), Gaps = 3/92 (3%)
Query: 241 SLRKHITACFSELACFLMP--HPGLKVATNPQFDGRLA-DIESEFKRSLQQFVPMLLAPE 297
+L K AC + C LM HPG FD + ++ +K F + +
Sbjct: 30 NLSKVEEACSGAVHCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITKH 89
Query: 298 NLVPKLINGQKVKAKDLVQYFKSYMNIYKGNE 329
V KL+ G+ + + +Q+ K Y + G +
Sbjct: 90 IEVSKLVKGRPLDNLEFMQWMKKYCDSVNGGQ 121
>At5g57035.1 68418.m07119 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 786
Score = 29.5 bits (63), Expect = 6.2
Identities = 47/202 (23%), Positives = 81/202 (40%), Gaps = 15/202 (7%)
Query: 206 RDWSFPYEAPYG-ADGGMQILSRRLKVSDKQHPELQSLRKHITACFSELACFLMPHPGLK 264
R S P+ P Q S + + D +H +S+ +H T S++ + P P LK
Sbjct: 230 RPGSLPHSHPTSRVYSDAQSSSTDIVLVDDEH--CRSILRHSTVSTSKIQ--MDPRPHLK 285
Query: 265 VATNPQFDGRLADIESEFKRSLQQFVPMLL-APENLVPKLINGQKVKAKDLVQYFKSYMN 323
+ G A++E + ++ +Q + M A E LV K Q + ++ + K
Sbjct: 286 TPKS----GVRAEVE-QLRKEVQTTLSMYKQACEELVHKQTQVQSLSSECI----KETER 336
Query: 324 IYKGNELPEPKSMLVATAEANNLTAVAEAKEVYTRLMEEVCGGGRPYLHADLLLTEHQRV 383
+ E E + A + +L AV E +E + L +E C L A E Q+V
Sbjct: 337 VITALEKEEMRRKAAAEEKEKHLKAVKEVEEAKSMLAKEFCERQLAELDALKQSIEKQKV 396
Query: 384 RDKAMYAFNAKRKMGGRDFSQA 405
++ RK + + A
Sbjct: 397 IEQLFLRDGRYRKYTKEEIAAA 418
>At1g05320.1 68414.m00539 myosin-related similar to non-muscle
myosin II heavy chain (GI:19879404) [Loligo pealei];
ESTs gb|AA042402,gb|ATTS1380 come from this gene
Length = 828
Score = 29.1 bits (62), Expect = 8.2
Identities = 14/50 (28%), Positives = 27/50 (54%)
Query: 275 LADIESEFKRSLQQFVPMLLAPENLVPKLINGQKVKAKDLVQYFKSYMNI 324
LAD ++ K +Q+ L + + + +N + +AKDL+ KS+ N+
Sbjct: 392 LADTNNQLKIKIQELEGYLDSEKETAIEKLNQKDTEAKDLITKLKSHENV 441
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.321 0.136 0.405
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,850,056
Number of Sequences: 28952
Number of extensions: 456037
Number of successful extensions: 968
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 961
Number of HSP's gapped (non-prelim): 13
length of query: 564
length of database: 12,070,560
effective HSP length: 85
effective length of query: 479
effective length of database: 9,609,640
effective search space: 4603017560
effective search space used: 4603017560
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 62 (29.1 bits)
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