BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000754-TA|BGIBMGA000754-PA|IPR005289|GTP-binding, IPR002917|GTP-binding protein, HSR1-related (333 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC25B2.04c |||mitochondrial ribosome assembly protein|Schizosa... 130 3e-31 SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2... 72 1e-13 SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 58 2e-09 SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyce... 40 4e-04 SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo... 36 0.006 SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 32 0.13 SPAC4F10.11 |spn1||septin Spn1|Schizosaccharomyces pombe|chr 1||... 29 0.93 SPBC3B9.03 |||signal recognition particle receptor alpha subunit... 29 1.2 SPAC821.06 |spn2||septin Spn2|Schizosaccharomyces pombe|chr 1|||... 29 1.2 SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces ... 28 1.6 SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe... 28 1.6 SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||M... 28 1.6 SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa... 28 2.1 SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch... 27 2.8 SPAC4D7.04c |||cis-prenyltransferase |Schizosaccharomyces pombe|... 27 3.7 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 27 4.9 SPAC6B12.10c |spp1|pri1|DNA primase catalytic subunit Spp1 |Schi... 27 4.9 SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|... 26 6.5 SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||... 26 6.5 SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 26 6.5 SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom... 26 8.6 >SPBC25B2.04c |||mitochondrial ribosome assembly protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 328 Score = 130 bits (314), Expect = 3e-31 Identities = 99/304 (32%), Positives = 147/304 (48%), Gaps = 25/304 (8%) Query: 26 WFPGHMNKGLKQMQRKLKSVDCVIEVHDARIPFTGRNPIFTSTLTGAKPHILVLNKRDLV 85 W+PGHMNK LK+++ S D +EV DARIP T RN + L K I+V NK DL Sbjct: 17 WYPGHMNKTLKRLKNLTSSNDIFVEVRDARIPLTSRNYVMEDFLN-KKNRIIVYNKCDLA 75 Query: 86 ITSLIP------RIKDQLKAEQNVD---NVVFTNSKDQFCRGLKTIKPLMVDLIKNSNRY 136 T RI++ + QNV+ T K F + P + R Sbjct: 76 DTFHTKAKVSKHRIQNLAQQFQNVECWFKETSTPEKSAFITPYVSKAPYFAKELLRLIRT 135 Query: 137 ---NRSEELEYNVMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKMRIN 193 S V +G+PN GKSS++N LR+ + VG GVT+ + +R+ Sbjct: 136 LVDQASANGRVYVYFVGMPNTGKSSILNSLRNVALRKSKSAIVGNYPGVTKRISEIVRLF 195 Query: 194 NDPCIFMLDTPGILEPSVTNIEMGLKLALCAALQDHLVGEEIIADYLLYWLNKHRKFKYV 253 ND ++MLDTPGI+ PS+T E LKL+L +++ +V + DYLL+ LN+ Y Sbjct: 196 NDMDVYMLDTPGIMTPSITKPEDMLKLSLVGCVKEGIVHPVTVVDYLLFHLNRIDPSLYS 255 Query: 254 DFMGLDEPCDDINKVLLSGAIKYNRIRKVRDFDGKVRDVPDLLETSRHIIKAFRTGELGK 313 + P +D+++ L + A K ++ K FD S ++I+ +R G LG+ Sbjct: 256 KW---SLPTNDVDEFLQNTAYKARKLTK-GGFDENF--------VSNYVIQQYRIGRLGR 303 Query: 314 VILD 317 LD Sbjct: 304 FQLD 307 >SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 470 Score = 72.1 bits (169), Expect = 1e-13 Identities = 65/225 (28%), Positives = 121/225 (53%), Gaps = 21/225 (9%) Query: 36 KQMQRKLKSVDCVIEVHDARIPFTGRNP-IFTSTLTGA---KPHILVLNKRDLVITSLIP 91 K+ ++ +++ D ++ V DAR P R+ + L + K I V+NK DLV + ++ Sbjct: 154 KEFKKVVEASDVILYVLDARDPEGTRSKDVERQVLASSAEEKRLIFVINKIDLVPSEVLN 213 Query: 92 RIKDQLK------AEQNVDNVVFTNSKDQFCRGLKTIKPLMVDLIKNSNRYNRSEELEYN 145 + L+ ++ +N K Q TI +L+K+ Y+ ++L+ + Sbjct: 214 KWVTYLRNFFPTIPMRSASGSGNSNLKHQSASASSTIS----NLLKSLKSYSAKKKLKSS 269 Query: 146 --VMIIGVPNVGKSSMINMLRSRNISGRHV-LPVGAVAGVTRSLMMKMRINNDPCIFMLD 202 V +IG PNVGKSS+IN L +R+ +GR P G VAG+T SL +++++N + ++D Sbjct: 270 LTVGVIGYPNVGKSSVINALVNRSANGRSAPCPAGNVAGMTTSL-REVKLDNK--LRLVD 326 Query: 203 TPGILEPSVTNIEMGLKLALCAALQDHLVGEEI-IADYLLYWLNK 246 +PGI+ PS + + +L + A+ V + + +A Y+L +L++ Sbjct: 327 SPGIVFPSSDSKDDLYRLVMLNAVSSTKVDDPVAVASYILQFLSR 371 >SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 58.0 bits (134), Expect = 2e-09 Identities = 63/247 (25%), Positives = 112/247 (45%), Gaps = 20/247 (8%) Query: 29 GHMNKGLKQMQRKLKSVDCVIEVHDARIPFTGRNPIFTSTLTGAKPH---ILVLNKRDLV 85 G + ++ + + S D +I+V DAR P R L H ILVLNK DLV Sbjct: 201 GQSKRIWNELYKVIDSSDVLIQVLDARDPVGTRCGTVERYLRNEASHKHMILVLNKVDLV 260 Query: 86 ITSLIPRIKDQLKAEQNVDNVVFTNSKDQFCRGLKTIKPLMVDLIKNSNRYNRSEELEYN 145 TS + ++ A++ + + F +G ++ +++ + S++ + + Sbjct: 261 PTS-VAAAWVKILAKEYPTIAFHASINNSFGKGS------LIQILRQFASLH-SDKKQIS 312 Query: 146 VMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKMRINNDPCIFMLDTPG 205 V +IG PN GKSS+IN LR + + +V P+ V + + + R IF++D PG Sbjct: 313 VGLIGFPNAGKSSIINTLRKKKVC--NVAPIPGETKVWQYVALMKR------IFLIDCPG 364 Query: 206 ILEPSVTNIEMGLKLALCAALQDHLVGEEIIADYLLYWLNKHRKFKYVDFMGLDEPCDDI 265 I+ PS + + L L +++ E I L KH + Y + G ++ + + Sbjct: 365 IVPPSSNDSDAELLLKGVVRVENVSNPEAYIPTVLSRCKVKHLERTY-EISGWNDSTEFL 423 Query: 266 NKVLLSG 272 K+ G Sbjct: 424 AKLAKKG 430 >SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 496 Score = 40.3 bits (90), Expect = 4e-04 Identities = 32/89 (35%), Positives = 50/89 (56%), Gaps = 9/89 (10%) Query: 145 NVMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTR-SLMMKMRINNDPCIFMLDT 203 NV I+G N GKSS+IN+L +R IS V +G TR ++ + + IN P + + DT Sbjct: 241 NVAILGPSNAGKSSLINLLANRRIS-----IVSPQSGTTRDAIEVLVDINGFP-VLLSDT 294 Query: 204 PGILE-PSVTNIE-MGLKLALCAALQDHL 230 G+ + V IE +G+++A A + L Sbjct: 295 AGLRKGEDVQEIEKIGIEIAKARAEESQL 323 >SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual Length = 616 Score = 36.3 bits (80), Expect = 0.006 Identities = 25/73 (34%), Positives = 35/73 (47%), Gaps = 8/73 (10%) Query: 148 IIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKMRINNDPCIFMLDTPGIL 207 ++G PNVGKSS IN L G + V + G T+ IN + +LD PG++ Sbjct: 306 LVGYPNVGKSSTINAL-----VGSKKVSVSSTPGKTKHF---QTINLSEKVSLLDCPGLV 357 Query: 208 EPSVTNIEMGLKL 220 PS + L L Sbjct: 358 FPSFATTQADLVL 370 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 31.9 bits (69), Expect = 0.13 Identities = 20/75 (26%), Positives = 41/75 (54%), Gaps = 2/75 (2%) Query: 259 DEPCDDINKVLLSGAIKYNRIRKV-RDFDGKVRDVPDLLETSRHIIKAFRTGELGKVILD 317 DE +D+ K+ + A K +++ + + K D+ LL++ R +++ TG+LGK+ + Sbjct: 295 DESSEDLEKLHSNFAEKVKEEQELYKSLEKKRSDLESLLKSRRELLEKL-TGDLGKIQGE 353 Query: 318 IDLLENRPSNKQEVL 332 I+ LE K ++ Sbjct: 354 IESLEKLKVKKSTMI 368 >SPAC4F10.11 |spn1||septin Spn1|Schizosaccharomyces pombe|chr 1|||Manual Length = 469 Score = 29.1 bits (62), Expect = 0.93 Identities = 17/82 (20%), Positives = 42/82 (51%), Gaps = 6/82 (7%) Query: 93 IKDQLKAEQNVDNVVFTNSKDQFCRGLKTIKPLM---VDLIKNSNRYNRS---EELEYNV 146 ++ + +N + + NS+ +F + I+ + V N+++R + +NV Sbjct: 39 VEAAISENKNEEKPIQDNSEQEFNPHVSIIQRQLNGYVGFASLPNQWHRRCVRQGFNFNV 98 Query: 147 MIIGVPNVGKSSMINMLRSRNI 168 +++G GKS+++N L +R++ Sbjct: 99 LVLGESGSGKSTLVNTLLNRDV 120 >SPBC3B9.03 |||signal recognition particle receptor alpha subunit Srp101|Schizosaccharomyces pombe|chr 2|||Manual Length = 547 Score = 28.7 bits (61), Expect = 1.2 Identities = 23/89 (25%), Positives = 40/89 (44%), Gaps = 3/89 (3%) Query: 75 HILVLNKRDLVITSLIPRIKDQL--KAEQNVDNVVFTNSKDQFCRGLKTIKP-LMVDLIK 131 H+ N + + L +K L K + D V T +K R + + P +DL+ Sbjct: 271 HLTKKNVANSIALELCESVKASLINKKVGSFDTVKNTVNKAFRDRLTQILTPSTSLDLLH 330 Query: 132 NSNRYNRSEELEYNVMIIGVPNVGKSSMI 160 + ++E Y + +IGV VGKS+ + Sbjct: 331 SIRSVRKNENRPYTISLIGVNGVGKSTTL 359 >SPAC821.06 |spn2||septin Spn2|Schizosaccharomyces pombe|chr 1|||Manual Length = 331 Score = 28.7 bits (61), Expect = 1.2 Identities = 11/26 (42%), Positives = 19/26 (73%) Query: 143 EYNVMIIGVPNVGKSSMINMLRSRNI 168 ++NVM++G GKS++IN L S ++ Sbjct: 32 QFNVMVVGPSGSGKSTLINTLFSAHL 57 >SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces pombe|chr 2|||Manual Length = 461 Score = 28.3 bits (60), Expect = 1.6 Identities = 21/105 (20%), Positives = 52/105 (49%), Gaps = 6/105 (5%) Query: 86 ITSLIPRIKDQLKAEQNVDNVVFTNSKDQFCRGLKTIKPLMVDLIKNSNRYNRSEELEYN 145 +T +I + +L ++ ++ V T+ + CR +K ++ +++L+K + E+E + Sbjct: 97 LTEIIGDVAGKLHTGRSRNDQVTTDLRLWLCRKIKEVEVYVINLLK---VFTNRAEMEID 153 Query: 146 VMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKM 190 V++ G ++ ++ + R + H LP+ G R L ++ Sbjct: 154 VIMSGYTHLQRAQPV---RWSHFLMSHALPLLGDLGRLRQLYTRV 195 >SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe|chr 2|||Manual Length = 675 Score = 28.3 bits (60), Expect = 1.6 Identities = 22/78 (28%), Positives = 38/78 (48%), Gaps = 5/78 (6%) Query: 258 LDEPCDDINKVL-LSGAIKYNRIRKVRDFDGKVRDVPDLLETSRHIIKAFRTGELGKVIL 316 L E D++ +L S RIR V DF+ + + L RH++K R ++ +L Sbjct: 245 LKETVDELIDILRYSPCTHQKRIRSVSDFERRWKLWRSRLANLRHVVKKHR--DIDSEVL 302 Query: 317 D--IDLLENRPSNKQEVL 332 D + LL+ NK+ ++ Sbjct: 303 DDFVVLLDILNGNKEVIM 320 >SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 407 Score = 28.3 bits (60), Expect = 1.6 Identities = 10/32 (31%), Positives = 18/32 (56%) Query: 148 IIGVPNVGKSSMINMLRSRNISGRHVLPVGAV 179 I+G+PN+GKS++ +L N+ P + Sbjct: 50 IVGMPNIGKSTLFQILTKTNLGNPANYPFATI 81 >SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 27.9 bits (59), Expect = 2.1 Identities = 21/63 (33%), Positives = 32/63 (50%), Gaps = 6/63 (9%) Query: 146 VMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKMRINNDPCIFMLDTPG 205 +++ G PNVGKSS +N + R + V A T+SL + ++DTPG Sbjct: 171 LLVCGYPNVGKSSFMNKVT------RAQVDVQPYAFTTKSLFVGHFDYKYLRWQVIDTPG 224 Query: 206 ILE 208 IL+ Sbjct: 225 ILD 227 >SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr 1|||Manual Length = 219 Score = 27.5 bits (58), Expect = 2.8 Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 5/68 (7%) Query: 74 PHILVLNKRDLVITSLIPRIKDQLKAEQNVDNVVFTNSKDQFCRGLKTIKPLMVDLIKNS 133 P +LV NK DL ++ R + + A+ V T++K + ++ L++ Sbjct: 115 PVVLVANKCDLEAERVVSRAEGEQLAKSMHCLYVETSAKLRL-----NVEEAFYSLVRTI 169 Query: 134 NRYNRSEE 141 RYN+SEE Sbjct: 170 RRYNKSEE 177 >SPAC4D7.04c |||cis-prenyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 264 Score = 27.1 bits (57), Expect = 3.7 Identities = 13/53 (24%), Positives = 29/53 (54%) Query: 203 TPGILEPSVTNIEMGLKLALCAALQDHLVGEEIIADYLLYWLNKHRKFKYVDF 255 TP ++ + + +K +L L G E ++D++L+ +K+ + K++DF Sbjct: 188 TPEDIDEDIFEKNLLIKDSLPLDLLIRTSGVERLSDFMLWQCHKNTEIKFIDF 240 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 26.6 bits (56), Expect = 4.9 Identities = 11/31 (35%), Positives = 17/31 (54%) Query: 168 ISGRHVLPVGAVAGVTRSLMMKMRINNDPCI 198 I+G LP+G AG+ L + R+N P + Sbjct: 1573 ITGSRKLPIGGFAGLNPPLTVVRRLNEPPYV 1603 >SPAC6B12.10c |spp1|pri1|DNA primase catalytic subunit Spp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 454 Score = 26.6 bits (56), Expect = 4.9 Identities = 9/37 (24%), Positives = 21/37 (56%) Query: 66 TSTLTGAKPHILVLNKRDLVITSLIPRIKDQLKAEQN 102 + ++ P ++ + K+D+V+T L PR+ ++ N Sbjct: 305 SGSIASISPSVIAIAKQDIVLTYLYPRLDVEVSRHLN 341 >SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|Schizosaccharomyces pombe|chr 2|||Manual Length = 1398 Score = 26.2 bits (55), Expect = 6.5 Identities = 17/70 (24%), Positives = 36/70 (51%), Gaps = 2/70 (2%) Query: 143 EYNVMIIGVPNVGKSSMI-NMLRSRNISGRHVLPVGAVAGVTRSLMMKMRINNDPCIFML 201 E+ +I+G+P GK++ I +++RS + +L ++++K++ D I L Sbjct: 948 EHYSLILGMPGTGKTTTISSLIRSLLAKKKKILLTSFTHLAVDNILIKLK-GCDSTIVRL 1006 Query: 202 DTPGILEPSV 211 +P + P V Sbjct: 1007 GSPHKIHPLV 1016 >SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2|||Manual Length = 205 Score = 26.2 bits (55), Expect = 6.5 Identities = 12/33 (36%), Positives = 20/33 (60%) Query: 146 VMIIGVPNVGKSSMINMLRSRNISGRHVLPVGA 178 V+I+G VGK+S++N +R S + +GA Sbjct: 11 VIILGESGVGKTSIMNQYVNRKFSKDYKATIGA 43 >SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1336 Score = 26.2 bits (55), Expect = 6.5 Identities = 11/31 (35%), Positives = 18/31 (58%) Query: 134 NRYNRSEELEYNVMIIGVPNVGKSSMINMLR 164 N + S E V I+G+ GKS+++ +LR Sbjct: 1119 NNVSLSIEAREKVAIVGISGSGKSTLVELLR 1149 >SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 25.8 bits (54), Expect = 8.6 Identities = 8/16 (50%), Positives = 14/16 (87%) Query: 148 IIGVPNVGKSSMINML 163 ++G+PN GKS+++N L Sbjct: 241 LVGLPNAGKSTLLNCL 256 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.323 0.140 0.412 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,486,957 Number of Sequences: 5004 Number of extensions: 61743 Number of successful extensions: 180 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 5 Number of HSP's that attempted gapping in prelim test: 159 Number of HSP's gapped (non-prelim): 24 length of query: 333 length of database: 2,362,478 effective HSP length: 73 effective length of query: 260 effective length of database: 1,997,186 effective search space: 519268360 effective search space used: 519268360 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 54 (25.8 bits)
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