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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000754-TA|BGIBMGA000754-PA|IPR005289|GTP-binding,
IPR002917|GTP-binding protein, HSR1-related
         (333 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC25B2.04c |||mitochondrial ribosome assembly protein|Schizosa...   130   3e-31
SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr 2...    72   1e-13
SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|...    58   2e-09
SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyce...    40   4e-04
SPAC3F10.16c |||GTP binding protein, HSR1-related|Schizosaccharo...    36   0.006
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos...    32   0.13 
SPAC4F10.11 |spn1||septin Spn1|Schizosaccharomyces pombe|chr 1||...    29   0.93 
SPBC3B9.03 |||signal recognition particle receptor alpha subunit...    29   1.2  
SPAC821.06 |spn2||septin Spn2|Schizosaccharomyces pombe|chr 1|||...    29   1.2  
SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces ...    28   1.6  
SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe...    28   1.6  
SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||M...    28   1.6  
SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1 |Schizosa...    28   2.1  
SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch...    27   2.8  
SPAC4D7.04c |||cis-prenyltransferase |Schizosaccharomyces pombe|...    27   3.7  
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig...    27   4.9  
SPAC6B12.10c |spp1|pri1|DNA primase catalytic subunit Spp1 |Schi...    27   4.9  
SPBC16D10.04c |dna2||DNA replication endonuclease-helicase Dna2|...    26   6.5  
SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr 2||...    26   6.5  
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo...    26   6.5  
SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom...    26   8.6  

>SPBC25B2.04c |||mitochondrial ribosome assembly
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 328

 Score =  130 bits (314), Expect = 3e-31
 Identities = 99/304 (32%), Positives = 147/304 (48%), Gaps = 25/304 (8%)

Query: 26  WFPGHMNKGLKQMQRKLKSVDCVIEVHDARIPFTGRNPIFTSTLTGAKPHILVLNKRDLV 85
           W+PGHMNK LK+++    S D  +EV DARIP T RN +    L   K  I+V NK DL 
Sbjct: 17  WYPGHMNKTLKRLKNLTSSNDIFVEVRDARIPLTSRNYVMEDFLN-KKNRIIVYNKCDLA 75

Query: 86  ITSLIP------RIKDQLKAEQNVD---NVVFTNSKDQFCRGLKTIKPLMVDLIKNSNRY 136
            T          RI++  +  QNV+       T  K  F     +  P     +    R 
Sbjct: 76  DTFHTKAKVSKHRIQNLAQQFQNVECWFKETSTPEKSAFITPYVSKAPYFAKELLRLIRT 135

Query: 137 ---NRSEELEYNVMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKMRIN 193
                S      V  +G+PN GKSS++N LR+  +       VG   GVT+ +   +R+ 
Sbjct: 136 LVDQASANGRVYVYFVGMPNTGKSSILNSLRNVALRKSKSAIVGNYPGVTKRISEIVRLF 195

Query: 194 NDPCIFMLDTPGILEPSVTNIEMGLKLALCAALQDHLVGEEIIADYLLYWLNKHRKFKYV 253
           ND  ++MLDTPGI+ PS+T  E  LKL+L   +++ +V    + DYLL+ LN+     Y 
Sbjct: 196 NDMDVYMLDTPGIMTPSITKPEDMLKLSLVGCVKEGIVHPVTVVDYLLFHLNRIDPSLYS 255

Query: 254 DFMGLDEPCDDINKVLLSGAIKYNRIRKVRDFDGKVRDVPDLLETSRHIIKAFRTGELGK 313
            +     P +D+++ L + A K  ++ K   FD            S ++I+ +R G LG+
Sbjct: 256 KW---SLPTNDVDEFLQNTAYKARKLTK-GGFDENF--------VSNYVIQQYRIGRLGR 303

Query: 314 VILD 317
             LD
Sbjct: 304 FQLD 307


>SPBC26H8.08c |grn1||GTPase Grn1 |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 470

 Score = 72.1 bits (169), Expect = 1e-13
 Identities = 65/225 (28%), Positives = 121/225 (53%), Gaps = 21/225 (9%)

Query: 36  KQMQRKLKSVDCVIEVHDARIPFTGRNP-IFTSTLTGA---KPHILVLNKRDLVITSLIP 91
           K+ ++ +++ D ++ V DAR P   R+  +    L  +   K  I V+NK DLV + ++ 
Sbjct: 154 KEFKKVVEASDVILYVLDARDPEGTRSKDVERQVLASSAEEKRLIFVINKIDLVPSEVLN 213

Query: 92  RIKDQLK------AEQNVDNVVFTNSKDQFCRGLKTIKPLMVDLIKNSNRYNRSEELEYN 145
           +    L+        ++      +N K Q      TI     +L+K+   Y+  ++L+ +
Sbjct: 214 KWVTYLRNFFPTIPMRSASGSGNSNLKHQSASASSTIS----NLLKSLKSYSAKKKLKSS 269

Query: 146 --VMIIGVPNVGKSSMINMLRSRNISGRHV-LPVGAVAGVTRSLMMKMRINNDPCIFMLD 202
             V +IG PNVGKSS+IN L +R+ +GR    P G VAG+T SL  +++++N   + ++D
Sbjct: 270 LTVGVIGYPNVGKSSVINALVNRSANGRSAPCPAGNVAGMTTSL-REVKLDNK--LRLVD 326

Query: 203 TPGILEPSVTNIEMGLKLALCAALQDHLVGEEI-IADYLLYWLNK 246
           +PGI+ PS  + +   +L +  A+    V + + +A Y+L +L++
Sbjct: 327 SPGIVFPSSDSKDDLYRLVMLNAVSSTKVDDPVAVASYILQFLSR 371


>SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 537

 Score = 58.0 bits (134), Expect = 2e-09
 Identities = 63/247 (25%), Positives = 112/247 (45%), Gaps = 20/247 (8%)

Query: 29  GHMNKGLKQMQRKLKSVDCVIEVHDARIPFTGRNPIFTSTLTGAKPH---ILVLNKRDLV 85
           G   +   ++ + + S D +I+V DAR P   R       L     H   ILVLNK DLV
Sbjct: 201 GQSKRIWNELYKVIDSSDVLIQVLDARDPVGTRCGTVERYLRNEASHKHMILVLNKVDLV 260

Query: 86  ITSLIPRIKDQLKAEQNVDNVVFTNSKDQFCRGLKTIKPLMVDLIKNSNRYNRSEELEYN 145
            TS +     ++ A++        +  + F +G       ++ +++     + S++ + +
Sbjct: 261 PTS-VAAAWVKILAKEYPTIAFHASINNSFGKGS------LIQILRQFASLH-SDKKQIS 312

Query: 146 VMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKMRINNDPCIFMLDTPG 205
           V +IG PN GKSS+IN LR + +   +V P+     V + + +  R      IF++D PG
Sbjct: 313 VGLIGFPNAGKSSIINTLRKKKVC--NVAPIPGETKVWQYVALMKR------IFLIDCPG 364

Query: 206 ILEPSVTNIEMGLKLALCAALQDHLVGEEIIADYLLYWLNKHRKFKYVDFMGLDEPCDDI 265
           I+ PS  + +  L L     +++    E  I   L     KH +  Y +  G ++  + +
Sbjct: 365 IVPPSSNDSDAELLLKGVVRVENVSNPEAYIPTVLSRCKVKHLERTY-EISGWNDSTEFL 423

Query: 266 NKVLLSG 272
            K+   G
Sbjct: 424 AKLAKKG 430


>SPAC222.05c |mss1||COX RNA-associated protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 496

 Score = 40.3 bits (90), Expect = 4e-04
 Identities = 32/89 (35%), Positives = 50/89 (56%), Gaps = 9/89 (10%)

Query: 145 NVMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTR-SLMMKMRINNDPCIFMLDT 203
           NV I+G  N GKSS+IN+L +R IS      V   +G TR ++ + + IN  P + + DT
Sbjct: 241 NVAILGPSNAGKSSLINLLANRRIS-----IVSPQSGTTRDAIEVLVDINGFP-VLLSDT 294

Query: 204 PGILE-PSVTNIE-MGLKLALCAALQDHL 230
            G+ +   V  IE +G+++A   A +  L
Sbjct: 295 AGLRKGEDVQEIEKIGIEIAKARAEESQL 323


>SPAC3F10.16c |||GTP binding protein,
           HSR1-related|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 616

 Score = 36.3 bits (80), Expect = 0.006
 Identities = 25/73 (34%), Positives = 35/73 (47%), Gaps = 8/73 (10%)

Query: 148 IIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKMRINNDPCIFMLDTPGIL 207
           ++G PNVGKSS IN L      G   + V +  G T+       IN    + +LD PG++
Sbjct: 306 LVGYPNVGKSSTINAL-----VGSKKVSVSSTPGKTKHF---QTINLSEKVSLLDCPGLV 357

Query: 208 EPSVTNIEMGLKL 220
            PS    +  L L
Sbjct: 358 FPSFATTQADLVL 370


>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
           Rad50|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1290

 Score = 31.9 bits (69), Expect = 0.13
 Identities = 20/75 (26%), Positives = 41/75 (54%), Gaps = 2/75 (2%)

Query: 259 DEPCDDINKVLLSGAIKYNRIRKV-RDFDGKVRDVPDLLETSRHIIKAFRTGELGKVILD 317
           DE  +D+ K+  + A K    +++ +  + K  D+  LL++ R +++   TG+LGK+  +
Sbjct: 295 DESSEDLEKLHSNFAEKVKEEQELYKSLEKKRSDLESLLKSRRELLEKL-TGDLGKIQGE 353

Query: 318 IDLLENRPSNKQEVL 332
           I+ LE     K  ++
Sbjct: 354 IESLEKLKVKKSTMI 368


>SPAC4F10.11 |spn1||septin Spn1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 469

 Score = 29.1 bits (62), Expect = 0.93
 Identities = 17/82 (20%), Positives = 42/82 (51%), Gaps = 6/82 (7%)

Query: 93  IKDQLKAEQNVDNVVFTNSKDQFCRGLKTIKPLM---VDLIKNSNRYNRS---EELEYNV 146
           ++  +   +N +  +  NS+ +F   +  I+  +   V      N+++R    +   +NV
Sbjct: 39  VEAAISENKNEEKPIQDNSEQEFNPHVSIIQRQLNGYVGFASLPNQWHRRCVRQGFNFNV 98

Query: 147 MIIGVPNVGKSSMINMLRSRNI 168
           +++G    GKS+++N L +R++
Sbjct: 99  LVLGESGSGKSTLVNTLLNRDV 120


>SPBC3B9.03 |||signal recognition particle receptor alpha subunit
           Srp101|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 547

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 23/89 (25%), Positives = 40/89 (44%), Gaps = 3/89 (3%)

Query: 75  HILVLNKRDLVITSLIPRIKDQL--KAEQNVDNVVFTNSKDQFCRGLKTIKP-LMVDLIK 131
           H+   N  + +   L   +K  L  K   + D V  T +K    R  + + P   +DL+ 
Sbjct: 271 HLTKKNVANSIALELCESVKASLINKKVGSFDTVKNTVNKAFRDRLTQILTPSTSLDLLH 330

Query: 132 NSNRYNRSEELEYNVMIIGVPNVGKSSMI 160
           +     ++E   Y + +IGV  VGKS+ +
Sbjct: 331 SIRSVRKNENRPYTISLIGVNGVGKSTTL 359


>SPAC821.06 |spn2||septin Spn2|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 331

 Score = 28.7 bits (61), Expect = 1.2
 Identities = 11/26 (42%), Positives = 19/26 (73%)

Query: 143 EYNVMIIGVPNVGKSSMINMLRSRNI 168
           ++NVM++G    GKS++IN L S ++
Sbjct: 32  QFNVMVVGPSGSGKSTLINTLFSAHL 57


>SPBC1773.14 |arg7||argininosuccinate lyase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 461

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 21/105 (20%), Positives = 52/105 (49%), Gaps = 6/105 (5%)

Query: 86  ITSLIPRIKDQLKAEQNVDNVVFTNSKDQFCRGLKTIKPLMVDLIKNSNRYNRSEELEYN 145
           +T +I  +  +L   ++ ++ V T+ +   CR +K ++  +++L+K    +    E+E +
Sbjct: 97  LTEIIGDVAGKLHTGRSRNDQVTTDLRLWLCRKIKEVEVYVINLLK---VFTNRAEMEID 153

Query: 146 VMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKM 190
           V++ G  ++ ++  +   R  +    H LP+    G  R L  ++
Sbjct: 154 VIMSGYTHLQRAQPV---RWSHFLMSHALPLLGDLGRLRQLYTRV 195


>SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 675

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 22/78 (28%), Positives = 38/78 (48%), Gaps = 5/78 (6%)

Query: 258 LDEPCDDINKVL-LSGAIKYNRIRKVRDFDGKVRDVPDLLETSRHIIKAFRTGELGKVIL 316
           L E  D++  +L  S      RIR V DF+ + +     L   RH++K  R  ++   +L
Sbjct: 245 LKETVDELIDILRYSPCTHQKRIRSVSDFERRWKLWRSRLANLRHVVKKHR--DIDSEVL 302

Query: 317 D--IDLLENRPSNKQEVL 332
           D  + LL+    NK+ ++
Sbjct: 303 DDFVVLLDILNGNKEVIM 320


>SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 407

 Score = 28.3 bits (60), Expect = 1.6
 Identities = 10/32 (31%), Positives = 18/32 (56%)

Query: 148 IIGVPNVGKSSMINMLRSRNISGRHVLPVGAV 179
           I+G+PN+GKS++  +L   N+      P   +
Sbjct: 50  IVGMPNIGKSTLFQILTKTNLGNPANYPFATI 81


>SPBC651.01c |nog1|SPBC725.18c|GTP binding protein Nog1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 642

 Score = 27.9 bits (59), Expect = 2.1
 Identities = 21/63 (33%), Positives = 32/63 (50%), Gaps = 6/63 (9%)

Query: 146 VMIIGVPNVGKSSMINMLRSRNISGRHVLPVGAVAGVTRSLMMKMRINNDPCIFMLDTPG 205
           +++ G PNVGKSS +N +       R  + V   A  T+SL +           ++DTPG
Sbjct: 171 LLVCGYPNVGKSSFMNKVT------RAQVDVQPYAFTTKSLFVGHFDYKYLRWQVIDTPG 224

Query: 206 ILE 208
           IL+
Sbjct: 225 ILD 227


>SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 219

 Score = 27.5 bits (58), Expect = 2.8
 Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 5/68 (7%)

Query: 74  PHILVLNKRDLVITSLIPRIKDQLKAEQNVDNVVFTNSKDQFCRGLKTIKPLMVDLIKNS 133
           P +LV NK DL    ++ R + +  A+      V T++K +       ++     L++  
Sbjct: 115 PVVLVANKCDLEAERVVSRAEGEQLAKSMHCLYVETSAKLRL-----NVEEAFYSLVRTI 169

Query: 134 NRYNRSEE 141
            RYN+SEE
Sbjct: 170 RRYNKSEE 177


>SPAC4D7.04c |||cis-prenyltransferase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 264

 Score = 27.1 bits (57), Expect = 3.7
 Identities = 13/53 (24%), Positives = 29/53 (54%)

Query: 203 TPGILEPSVTNIEMGLKLALCAALQDHLVGEEIIADYLLYWLNKHRKFKYVDF 255
           TP  ++  +    + +K +L   L     G E ++D++L+  +K+ + K++DF
Sbjct: 188 TPEDIDEDIFEKNLLIKDSLPLDLLIRTSGVERLSDFMLWQCHKNTEIKFIDF 240


>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
            E3 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1647

 Score = 26.6 bits (56), Expect = 4.9
 Identities = 11/31 (35%), Positives = 17/31 (54%)

Query: 168  ISGRHVLPVGAVAGVTRSLMMKMRINNDPCI 198
            I+G   LP+G  AG+   L +  R+N  P +
Sbjct: 1573 ITGSRKLPIGGFAGLNPPLTVVRRLNEPPYV 1603


>SPAC6B12.10c |spp1|pri1|DNA primase catalytic subunit Spp1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 454

 Score = 26.6 bits (56), Expect = 4.9
 Identities = 9/37 (24%), Positives = 21/37 (56%)

Query: 66  TSTLTGAKPHILVLNKRDLVITSLIPRIKDQLKAEQN 102
           + ++    P ++ + K+D+V+T L PR+  ++    N
Sbjct: 305 SGSIASISPSVIAIAKQDIVLTYLYPRLDVEVSRHLN 341


>SPBC16D10.04c |dna2||DNA replication endonuclease-helicase
            Dna2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1398

 Score = 26.2 bits (55), Expect = 6.5
 Identities = 17/70 (24%), Positives = 36/70 (51%), Gaps = 2/70 (2%)

Query: 143  EYNVMIIGVPNVGKSSMI-NMLRSRNISGRHVLPVGAVAGVTRSLMMKMRINNDPCIFML 201
            E+  +I+G+P  GK++ I +++RS     + +L          ++++K++   D  I  L
Sbjct: 948  EHYSLILGMPGTGKTTTISSLIRSLLAKKKKILLTSFTHLAVDNILIKLK-GCDSTIVRL 1006

Query: 202  DTPGILEPSV 211
             +P  + P V
Sbjct: 1007 GSPHKIHPLV 1016


>SPBC405.04c |ypt7||GTPase Ypt7|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 205

 Score = 26.2 bits (55), Expect = 6.5
 Identities = 12/33 (36%), Positives = 20/33 (60%)

Query: 146 VMIIGVPNVGKSSMINMLRSRNISGRHVLPVGA 178
           V+I+G   VGK+S++N   +R  S  +   +GA
Sbjct: 11  VIILGESGVGKTSIMNQYVNRKFSKDYKATIGA 43


>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1336

 Score = 26.2 bits (55), Expect = 6.5
 Identities = 11/31 (35%), Positives = 18/31 (58%)

Query: 134  NRYNRSEELEYNVMIIGVPNVGKSSMINMLR 164
            N  + S E    V I+G+   GKS+++ +LR
Sbjct: 1119 NNVSLSIEAREKVAIVGISGSGKSTLVELLR 1149


>SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 419

 Score = 25.8 bits (54), Expect = 8.6
 Identities = 8/16 (50%), Positives = 14/16 (87%)

Query: 148 IIGVPNVGKSSMINML 163
           ++G+PN GKS+++N L
Sbjct: 241 LVGLPNAGKSTLLNCL 256


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.323    0.140    0.412 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,486,957
Number of Sequences: 5004
Number of extensions: 61743
Number of successful extensions: 180
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 159
Number of HSP's gapped (non-prelim): 24
length of query: 333
length of database: 2,362,478
effective HSP length: 73
effective length of query: 260
effective length of database: 1,997,186
effective search space: 519268360
effective search space used: 519268360
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 54 (25.8 bits)

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