Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000742-TA|BGIBMGA000742-PA|IPR003877|SPla/RYanodine
receptor SPRY, IPR001870|B302, (SPRY)-like
         (546 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g22010.1 68415.m02614 zinc finger (C3HC4-type RING finger) fa...    83   6e-16
At1g51450.1 68414.m05791 SPla/RYanodine receptor (SPRY) domain-c...    42   8e-04
At4g09340.1 68417.m01543 SPla/RYanodine receptor (SPRY) domain-c...    34   0.28 
At4g30990.1 68417.m04398 expressed protein ; expression supporte...    32   1.1  
At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl...    30   4.5  
At2g38185.4 68415.m04692 zinc finger (C3HC4-type RING finger) fa...    29   6.0  

>At2g22010.1 68415.m02614 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profile: PF00097 zinc
           finger, C3HC4 type (RING finger)
          Length = 1280

 Score = 82.6 bits (195), Expect = 6e-16
 Identities = 53/147 (36%), Positives = 66/147 (44%), Gaps = 12/147 (8%)

Query: 297 FESVRCTFQVDNGCWYYEVTIVTPGVMQIGWATKNSHFLNHEGYGIGDDLYSLSYDGCRK 356
           F S R    +  G W YEV + T G+ Q+GWAT    F + +G G  DD Y  ++DG R 
Sbjct: 135 FSSARANACIWKGKWMYEVALETSGIQQLGWATLACPFTDQKGVGDADDSY--AFDGRRV 192

Query: 357 LIWYK-AKPASVQDLPEWQPGDILGCLIDLNTREAIFSLNGRCLTPCTEIFETTRCCKQG 415
             W K A+P        W  GD++GC IDLN  E  F  NG  L      F   R    G
Sbjct: 193 SKWNKEAEPYG----QSWVAGDVIGCCIDLNCDEIYFYRNGVSLGAA---FTGIRKLGPG 245

Query: 416 XXXXXXXXXXQ--QCRFNFGHLPFSFP 440
                     Q  +C  NFG  PF +P
Sbjct: 246 FGYYPAISLSQGERCELNFGAYPFKYP 272


>At1g51450.1 68414.m05791 SPla/RYanodine receptor (SPRY)
           domain-containing protein low similarity to DEAD box
           protein DDX1 [Gallus gallus] GI:16323037,  ryanodine
           receptor [Caenorhabditis elegans] GI:1871447; contains
           Pfam profile PF00622: SPRY domain
          Length = 509

 Score = 42.3 bits (95), Expect = 8e-04
 Identities = 39/149 (26%), Positives = 71/149 (47%), Gaps = 10/149 (6%)

Query: 246 ALDNLFVIENRPLSYETVDMTP-INVILNSHDVSEYLKISCNGLEARCDSYSFESVRCTF 304
           A+++  +I   P   +  D TP + + L+    +E ++IS + L A   S  +  VR T 
Sbjct: 248 AVEDKVLITPVPRFPDKGDDTPDLEICLSKVYKAEKVEISEDRLTAG-SSKGYRMVRATR 306

Query: 305 QVDNGCWYYEVTIVT---PGVMQIGWATKNSHFLNHEGYGIGDDLYSLSYDGCRKLIWYK 361
            V  G WY+E+ +++    G  ++GW+T         GY  G+       DGC+    +K
Sbjct: 307 GVVEGAWYFEIKVLSLGETGHTRLGWSTDKGDLQAPVGYD-GNSFGFRDIDGCK---IHK 362

Query: 362 AKPASVQDLPEWQPGDILGCLIDLNTREA 390
           A   +  +   ++ GD++G  I+L   E+
Sbjct: 363 ALRETYAE-EGYKEGDVIGFYINLPDGES 390


>At4g09340.1 68417.m01543 SPla/RYanodine receptor (SPRY)
           domain-containing protein low similarity to RanBPM [Homo
           sapiens] GI:15080674; contains Pfam profile PF00622:
           SPRY domain
          Length = 447

 Score = 33.9 bits (74), Expect = 0.28
 Identities = 25/89 (28%), Positives = 43/89 (48%), Gaps = 8/89 (8%)

Query: 311 WYYEVTIVTPGV---MQIGWATKNSHFLNHEGYGIGDDLYSLSYDGCRKLIWYKAKPASV 367
           +Y+E+ +   GV   + IG+ T +     H G+    +L +  Y G   LI Y  K    
Sbjct: 79  YYFEIYVKDAGVKGRVSIGFTTNSFRIARHPGW----ELNTCGYHGEDGLI-YLGKRQGE 133

Query: 368 QDLPEWQPGDILGCLIDLNTREAIFSLNG 396
              P +  GD +G  I+ +++E  F++NG
Sbjct: 134 AFGPTYTTGDTVGGGINYDSQEFFFTVNG 162


>At4g30990.1 68417.m04398 expressed protein ; expression supported by
            MPSS
          Length = 2138

 Score = 31.9 bits (69), Expect = 1.1
 Identities = 19/73 (26%), Positives = 41/73 (56%)

Query: 24   SASMSLSPGDARLRAMKALDPSIVDQLVMEMLTLIASFVDNDEESPVLLVKLHVIADKEE 83
            S +++++   A L+ +K L   ++D  +  ++  IASF+ N  ES     +L ++A  +E
Sbjct: 1586 SDNVNVNSSVAALKVLKLLPEDVLDSNLSSIVHKIASFLKNRLESTRDEARLALVACLKE 1645

Query: 84   GWLQVVSSMVNVI 96
              L+ +  +VN++
Sbjct: 1646 LGLEYLQVVVNIL 1658


>At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly
            identical to ubiquitin-protein ligase 2 [Arabidopsis
            thaliana] GI:7108523; E3, HECT-domain protein family;
            similar to ubiquitin-protein ligase 2 GI:7108523 from
            [Arabidopsis thaliana]
          Length = 3658

 Score = 29.9 bits (64), Expect = 4.5
 Identities = 11/27 (40%), Positives = 20/27 (74%)

Query: 84   GWLQVVSSMVNVIPLDNPLGPSAITIV 110
            G +Q ++S++NVI LD+P  P  +T++
Sbjct: 1964 GTIQCLTSILNVIDLDHPDAPKLVTLI 1990


>At2g38185.4 68415.m04692 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam profile PF00097: Zinc
           finger, C3HC4 type (RING finger)
          Length = 872

 Score = 29.5 bits (63), Expect = 6.0
 Identities = 9/21 (42%), Positives = 13/21 (61%)

Query: 479 ENACTLCYDAVADCTLEPCFH 499
           ++ C +C+DA  DC   PC H
Sbjct: 818 KSLCAICFDAPRDCCFLPCGH 838


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.322    0.137    0.429 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,196,809
Number of Sequences: 28952
Number of extensions: 561107
Number of successful extensions: 1177
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1169
Number of HSP's gapped (non-prelim): 8
length of query: 546
length of database: 12,070,560
effective HSP length: 85
effective length of query: 461
effective length of database: 9,609,640
effective search space: 4430044040
effective search space used: 4430044040
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 62 (29.1 bits)