Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000722-TA|BGIBMGA000722-PA|IPR002994|Surfeit locus 1
         (257 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g17910.1 68416.m02281 surfeit 1 (SURF1) identical to Surfeit ...    82   4e-16
At1g48510.1 68414.m05422 cytochrome c oxidase assembly protein s...    31   0.58 
At2g17290.1 68415.m01997 calcium-dependent protein kinase isofor...    30   1.8  
At1g66500.1 68414.m07554 zinc finger (C2H2-type) family protein ...    29   3.1  
At5g43620.1 68418.m05332 S-locus protein-related contains some s...    29   4.1  
At3g26300.1 68416.m03282 cytochrome P450 family protein contains...    28   5.4  
At2g25470.1 68415.m03050 leucine-rich repeat family protein cont...    28   5.4  
At1g50820.1 68414.m05715 hypothetical protein                          28   5.4  

>At3g17910.1 68416.m02281 surfeit 1 (SURF1) identical to Surfeit 1
           GB:AAF19609 from [Arabidopsis thaliana]
          Length = 354

 Score = 81.8 bits (193), Expect = 4e-16
 Identities = 80/276 (28%), Positives = 125/276 (45%), Gaps = 46/276 (16%)

Query: 17  KW--ILLMIP-VTSFTLGSWQVYRWQWKLGLIDMMQAKSNAVPI----DMPKDFSELEKM 69
           KW  +LL +P   +F LGSWQ+ R + K   ++  Q + N  PI    D P D   L  +
Sbjct: 72  KWSQLLLFLPGAITFGLGSWQIVRREEKFKTLEYQQQRLNMEPIKLNIDHPLD-KNLNAL 130

Query: 70  EYLPVKVKGEFLHEKEILIGPRAL----IEESS---------ITNRVGSLVSDPKKNQGW 116
           E+  V  KG F  ++ I +GPR+     I E+          I   + S+ S    N+GW
Sbjct: 131 EFRRVSCKGVFDEQRSIYLGPRSRSISGITENGFFVITPLMPIPGDLDSMQSPILVNRGW 190

Query: 117 LVIT-PFKLADTGEVILI-NRG-------------WIHQNLRPKEKREPSLIKGPVELTG 161
           +  +   K  ++ E   I N+              W   +  P   +E      PVE+ G
Sbjct: 191 VPRSWREKSQESAEAEFIANQSTKAKSPSNEPKSWWKFWSKTPVITKEHISAVKPVEVVG 250

Query: 162 VVRLTEKRAPFMPKNNPEKGSWFYRDLDQMSAHIGCLP---IWL-DAKGIPDPPTGWPIP 217
           V+R  E  + F+P N+P  G WFY D+  M+  +G LP   I++ D     D    +P+P
Sbjct: 251 VIRGGENPSIFVPSNDPSTGQWFYVDVPAMARAVG-LPENTIYVEDVHEHVDRSRPYPVP 309

Query: 218 NQTRVTLRN-----EHFSYIVTWYSLFAFTSIMWHR 248
                 +R+     +H +Y +TWYSL A  + M ++
Sbjct: 310 KDINTLIRSKVMPQDHLNYSITWYSLSAAVTFMAYK 345


>At1g48510.1 68414.m05422 cytochrome c oxidase assembly protein
           surfeit-related contains similarity to Swiss-Prot:Q9QXU2
           surfeit locus protein 1 [Rattus norvegicus]
          Length = 384

 Score = 31.5 bits (68), Expect = 0.58
 Identities = 14/40 (35%), Positives = 23/40 (57%)

Query: 157 VELTGVVRLTEKRAPFMPKNNPEKGSWFYRDLDQMSAHIG 196
           VE+ GVVR +E    +   N P   +WFY D+ +++  +G
Sbjct: 227 VEVVGVVRKSETPGIYTLVNYPSSLAWFYLDVPKLALAMG 266



 Score = 29.1 bits (62), Expect = 3.1
 Identities = 31/140 (22%), Positives = 61/140 (43%), Gaps = 11/140 (7%)

Query: 1   MRSQKVKRKEEPTEIYKWILLMIPVTSFTL-GSWQVYRW-QWKLGLIDMMQAKSNAVPID 58
           + SQ +     P +  +   L+  +  FT  G  + Y++ Q ++  +D  +      P+ 
Sbjct: 36  LESQLLSSAPPPAKKKRGSALLWYLVGFTTYGLGETYKFLQTQVEHLDSRKQCLEMKPMK 95

Query: 59  MPKDFSELEKMEYLPVKVKGEFLHEKEILIGPRALIEESSITNRVGSLVSDPKKNQGWLV 118
           +     +L+ + +  V  KG F  ++ I +GP+      S ++ +G  V  P      L+
Sbjct: 96  L-NTTKDLDGLGFRRVVCKGIFDEQRSIYVGPKP--RSMSKSSEIGFYVITP------LL 146

Query: 119 ITPFKLADTGEVILINRGWI 138
             P +       IL+NRGW+
Sbjct: 147 PIPNEPNSMKSPILVNRGWV 166


>At2g17290.1 68415.m01997 calcium-dependent protein kinase isoform 6
           (CPK6) identical to calmodulin-domain protein kinase
           CDPK isoform 6 [Arabidopsis thaliana]
           gi|1399275|gb|AAB03246; contains protein kinase domain,
           Pfam:PF00069; contains EF hand domain (calcium-binding
           EF-hand), Pfam:PF00036, INTERPRO:IPR002048
          Length = 544

 Score = 29.9 bits (64), Expect = 1.8
 Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 2/52 (3%)

Query: 61  KDFSELEKMEYLPVKVKGEFLHEKEILIGPRALIEESSITNRVGSLVSDPKK 112
           K FS + K++ + +KV  E L E+EI  G RA+ E    T+  G++  D  K
Sbjct: 363 KQFSAMNKLKKMALKVIAESLSEEEI-AGLRAMFEAMD-TDNSGAITFDELK 412


>At1g66500.1 68414.m07554 zinc finger (C2H2-type) family protein
           contains Prosite PS00028: Zinc finger, C2H2 type,
           domain; similar to S-locus protein 4 (GI:6069478)
           [Brassica rapa]; similar to Pre-mRNA cleavage complex II
           protein Pcf11 (Fragment) (Swiss-Prot:O94913) [Homo
           sapiens]
          Length = 416

 Score = 29.1 bits (62), Expect = 3.1
 Identities = 31/119 (26%), Positives = 50/119 (42%), Gaps = 13/119 (10%)

Query: 100 TNRVGSLVSDPKKNQGWLVITPFKL--ADTGEVI-LINRGWIHQNLRPKEKREPSLIKGP 156
           T R+G     PKK++GWL      L  A  GE + + + G   Q  + K++ EP  +  P
Sbjct: 286 TTRLGQ---QPKKSRGWLASASLWLCAATGGETVEVASFGGEMQKKKGKDE-EPKQLMVP 341

Query: 157 VELTGVVRLTEKRAPFMPKNNPEKGSWFYRDLDQMS-----AHIGCLPIWLDAKGIPDP 210
            +            PF    + E   W Y+D   ++      H+ C+P    AK + +P
Sbjct: 342 AD-EDQKNCALCVEPFEEFFSHEDDDWMYKDAVYLTKNGRIVHVKCMPEPRPAKDLREP 399


>At5g43620.1 68418.m05332 S-locus protein-related contains some
           similarity to S-locus protein 4 GI:6069478 from
           [Brassica rapa]
          Length = 410

 Score = 28.7 bits (61), Expect = 4.1
 Identities = 29/121 (23%), Positives = 48/121 (39%), Gaps = 16/121 (13%)

Query: 100 TNRVGSLVSDPKKNQGWLVITPFKL-ADTG----EVILINRGWIHQNLRPKEKREPSLIK 154
           T R+G     PKK++GWL      L A TG    EV     G + +     + ++  ++ 
Sbjct: 279 TTRLGQ---QPKKSRGWLASASLWLCAPTGGGTVEVASFGGGEMQKKNEKDQVQKQHMVP 335

Query: 155 GPVELTGVVRLTEKRAPFMPKNNPEKGSWFYRDLDQMS-----AHIGCLPIWLDAKGIPD 209
              +        E   PF    + E   W Y+D   ++      H+ C+P    AK + +
Sbjct: 336 ADEDQKNCALCVE---PFEEFFSHEADDWMYKDAVYLTKNGRIVHVKCMPEPRPAKDLRE 392

Query: 210 P 210
           P
Sbjct: 393 P 393


>At3g26300.1 68416.m03282 cytochrome P450 family protein contains
           Pfam profile: PF00067 cytochrome P450
          Length = 500

 Score = 28.3 bits (60), Expect = 5.4
 Identities = 21/73 (28%), Positives = 37/73 (50%)

Query: 7   KRKEEPTEIYKWILLMIPVTSFTLGSWQVYRWQWKLGLIDMMQAKSNAVPIDMPKDFSEL 66
           ++KEE +E +  +LL +      LG+ ++ R   K  L+D++ A  +   I M    +EL
Sbjct: 258 QKKEEGSEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAGMDTSAITMTWAMAEL 317

Query: 67  EKMEYLPVKVKGE 79
            K   +  KV+ E
Sbjct: 318 AKNPRVMKKVQSE 330


>At2g25470.1 68415.m03050 leucine-rich repeat family protein
           contains leucine rich-repeat (LRR) domains Pfam:PF00560,
           INTERPRO:IPR001611; contains similarity to disease
           resistance protein [Lycopersicon esculentum]
           gi|3894383|gb|AAC78591
          Length = 910

 Score = 28.3 bits (60), Expect = 5.4
 Identities = 14/32 (43%), Positives = 20/32 (62%), Gaps = 3/32 (9%)

Query: 41  KLGLIDMMQAKSNAVPIDMPKDFSELEKMEYL 72
           KL ++D+    SN +  D+P  FS LE +EYL
Sbjct: 246 KLRVLDL---SSNQLSGDLPSSFSSLESLEYL 274


>At1g50820.1 68414.m05715 hypothetical protein
          Length = 528

 Score = 28.3 bits (60), Expect = 5.4
 Identities = 10/21 (47%), Positives = 14/21 (66%)

Query: 227 EHFSYIVTWYSLFAFTSIMWH 247
           EH  ++V W S FAF S ++H
Sbjct: 186 EHVGFLVLWLSYFAFPSHLFH 206


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.321    0.139    0.445 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,727,476
Number of Sequences: 28952
Number of extensions: 300234
Number of successful extensions: 619
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 610
Number of HSP's gapped (non-prelim): 10
length of query: 257
length of database: 12,070,560
effective HSP length: 80
effective length of query: 177
effective length of database: 9,754,400
effective search space: 1726528800
effective search space used: 1726528800
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 58 (27.5 bits)