Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000721-TA|BGIBMGA000721-PA|IPR011904|Acetate--CoA
ligase, IPR000873|AMP-dependent synthetase and ligase
(714 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative / aceta... 459 e-129
At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearl... 117 3e-26
At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase fam... 84 3e-16
At5g16370.1 68418.m01913 AMP-binding protein, putative similar t... 83 8e-16
At1g75960.1 68414.m08822 AMP-binding protein, putative similar t... 82 1e-15
At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearl... 81 3e-15
At5g16340.1 68418.m01910 AMP-binding protein, putative similar t... 79 1e-14
At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein ... 77 5e-14
At1g76290.1 68414.m08860 AMP-dependent synthetase and ligase fam... 75 2e-13
At1g21540.1 68414.m02694 AMP-binding protein, putative strong si... 71 3e-12
At1g21530.1 68414.m02693 AMP-binding protein, putative strong si... 71 3e-12
At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 71 4e-12
At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase fam... 70 5e-12
At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ... 70 6e-12
At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase fam... 69 8e-12
At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase fam... 69 1e-11
At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 69 1e-11
At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein ... 69 1e-11
At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein ... 68 3e-11
At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) simil... 68 3e-11
At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ... 67 4e-11
At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase fam... 66 6e-11
At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) simil... 65 2e-10
At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c... 63 7e-10
At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) simil... 63 7e-10
At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase famil... 62 9e-10
At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase fam... 62 9e-10
At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein ... 62 1e-09
At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 / 4-coumaroyl... 62 1e-09
At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl... 61 3e-09
At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 60 7e-09
At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase fam... 52 1e-06
At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase famil... 52 1e-06
At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase famil... 52 1e-06
At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein ... 51 3e-06
At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase fam... 48 2e-05
At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl... 46 7e-05
At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase / lon... 42 0.001
At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase famil... 42 0.001
At1g20500.1 68414.m02554 4-coumarate--CoA ligase family / 4-coum... 42 0.001
At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase, puta... 42 0.002
At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase, puta... 42 0.002
At4g14070.1 68417.m02172 AMP-binding protein, putative similar t... 41 0.002
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 40 0.004
At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14) ident... 39 0.010
At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 / 4-coumaroyl... 38 0.023
At3g23790.1 68416.m02990 AMP-binding protein, putative similar t... 38 0.031
At5g27600.1 68418.m03305 AMP-binding protein, putative similar t... 37 0.053
At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase / lon... 34 0.29
At3g58200.1 68416.m06489 meprin and TRAF homology domain-contain... 32 1.5
At5g15680.1 68418.m01834 expressed protein 30 6.1
At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NA... 30 6.1
At3g49260.2 68416.m05384 calmodulin-binding family protein low s... 30 6.1
At3g49260.1 68416.m05383 calmodulin-binding family protein low s... 30 6.1
At3g54940.3 68416.m06091 cysteine proteinase, putative contains ... 29 8.1
At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu... 29 8.1
>At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative /
acetate-CoA ligase, putative similar to SP|P27550
(Escherichia coli) and gi:8439651 (Homo sapiens);
contains Pfam AMP-binding enzyme domain PF00501
Length = 693
Score = 459 bits (1132), Expect = e-129
Identities = 216/454 (47%), Positives = 292/454 (64%), Gaps = 14/454 (3%)
Query: 8 PTDHVIKKSYLPSIQKYKEMYERSLTESELFWTEVAQEFQWHVAPKPGGFMKYNFDITKG 67
P+ ++ + S Q+Y EM++RS+ + FW+++A EF W F + N D+ KG
Sbjct: 44 PSKEFSGQALVSSPQQYMEMHKRSMDDPAAFWSDIASEFYWKQKWGDQVFSE-NLDVRKG 102
Query: 68 RVFVKFMEEAELNVCYNLLDRNVKNGFGDKIAFFWEGNSPDDYTRITYRKLLDLVCQFAN 127
+ +++ + N+CYN LD+NV+ G GDK A WEGN +TY +LL VCQ AN
Sbjct: 103 PISIEWFKGGITNICYNCLDKNVEAGLGDKTAIHWEGNELGVDASLTYSELLQRVCQLAN 162
Query: 128 GLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALAERLSDAKAKL 187
L G++KGD V +Y+PM++E I MLACARIGAVH+VVFAGFS+D+LA+R+ D K +
Sbjct: 163 YLKDNGVKKGDAVVIYLPMLMELPIAMLACARIGAVHSVVFAGFSADSLAQRIVDCKPNV 222
Query: 188 LVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNHLDRVTPGGSLKSEIKDL 247
++T + RG + K D A++++ V +C+ ++ T ++
Sbjct: 223 ILTCNAVKRGPKTINLKAIVDAALDQSSKDGVS-VGICLTYDNSLATT---------REN 272
Query: 248 YNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAA 307
W D+WW +V+ C EWV AEDPLF+LYTSGSTGKPKGV+HTT GY++Y A
Sbjct: 273 TKWQNGRDVWWQDVISQYPTSCEVEWVDAEDPLFLLYTSGSTGKPKGVLHTTGGYMIYTA 332
Query: 308 ATFRYVFDYHRRDVYWCTADVGWITGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSII 367
TF+Y FDY DVYWCTAD GWITGH+YV Y P+ N AT V+FEG P +P R W I+
Sbjct: 333 TTFKYAFDYKSTDVYWCTADCGWITGHSYVTYGPMLNGATVVVFEGAPNYPDPGRCWDIV 392
Query: 368 NKYKVTQFYTAPTAIRALMAHYNVEDGNLTMPDLSSLRVLGSVGEPINPAAWLWYYTRIG 427
+KYKV+ FYTAPT +R+LM +D +T SLRVLGSVGEPINP+AW W++ +G
Sbjct: 393 DKYKVSIFYTAPTLVRSLMRD---DDKFVTRHSRKSLRVLGSVGEPINPSAWRWFFNVVG 449
Query: 428 KERCSIVDTFWQTETGGHVITGLPGATPMKPGAA 461
RC I DT+WQTETGG +IT LPGA P KPG+A
Sbjct: 450 DSRCPISDTWWQTETGGFMITPLPGAWPQKPGSA 483
Score = 255 bits (625), Expect = 6e-68
Identities = 127/220 (57%), Positives = 153/220 (69%), Gaps = 4/220 (1%)
Query: 486 MIVHLPNKSNRTRG---FPFFGVEPKILNEDGVEIEGPGEGYLVFARPWPGMMRTLLEDQ 542
MI LP + G FPFFGV+P I++E G EIEG GYL WPG RTL D
Sbjct: 468 MITPLPGAWPQKPGSATFPFFGVQPVIVDEKGNEIEGECSGYLCVKGSWPGAFRTLFGDH 527
Query: 543 DRFERVYFSKFPGYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPR 602
+R+E YF F GYY +GDG RD+DGY W+TGR+DD++NVSGH + TAEVE L P+
Sbjct: 528 ERYETTYFKPFAGYYFSGDGCSRDKDGYYWLTGRVDDVINVSGHRIGTAEVESALVLHPQ 587
Query: 603 VSEAAVVPKPHEVKGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPG 662
+EAAVV HEVKG+ +Y FV L EG+ + +L K+L L+VR +IGAFAAPD I APG
Sbjct: 588 CAEAAVVGIEHEVKGQGIYAFVTLLEGVPYSEELRKSLVLMVRNQIGAFAAPDRIHWAPG 647
Query: 663 LPKTRSGKIMRRILRKIAVGD-HEVGDISTLADPGVVEEL 701
LPKTRSGKIMRRILRKIA E+GD STLADP VV++L
Sbjct: 648 LPKTRSGKIMRRILRKIASRQLEELGDTSTLADPSVVDQL 687
>At5g23050.1 68418.m02695 acyl-activating enzyme 17 (AAE17) nearly
identical to acyl-activating enzyme 17 [Arabidopsis
thaliana] GI:29893266; similar to acetyl-CoA synthetase
[SP|P27095] from Methanothrix soehngenii; contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-activating enzyme 17 (At5g23050) GI:29893265
Length = 721
Score = 117 bits (281), Expect = 3e-26
Identities = 118/440 (26%), Positives = 188/440 (42%), Gaps = 43/440 (9%)
Query: 16 SYLPSIQKYKEMYERSLTESELFWTEVAQEFQWHVAPKPGGFMKYNFDITKGRVFVKFME 75
+Y + + E S++ E++W V E + P ++ D + K++
Sbjct: 107 NYKNPVSSFSSFQEFSVSNPEVYWKTVLDELNILFSVPPKCILEK--DTSGDNPGGKWLP 164
Query: 76 EAELNVCYNLLDRNVKNGFGDKIAFFWEGNSPDDY--TRITYRKLLDLVCQFANGLLAKG 133
A LN N L K D I W DD +T +L V A+ L A G
Sbjct: 165 GAYLNPARNCLTNGFKRRLDD-IVIRWRDEGSDDLPVNTMTLLELRSQVWLAAHALSALG 223
Query: 134 IRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALAERLSDAKAKLLVTADG 193
+ + +AV MPM +E+VI LA G V + FS ++ RL +KAK + T D
Sbjct: 224 LEEESAIAVDMPMNVESVIIYLAIVLAGHVVVSIADSFSPREISTRLKISKAKAIFTQDV 283
Query: 194 AWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNHLDRVTPGGSLKSEIKDLYNWNEE 253
RG++ R DA + +VV G S + +++ E
Sbjct: 284 IIRGDKSIPLYRRVVDA----------EAPLAIVVP-----ARGSSCRMKLR-------E 321
Query: 254 TDIWWHEVLEGESNICPPEWVAAEDPLF----VLYTSGSTGKPKGVVHTTAGYLMYAAAT 309
D+ W+ L N+ E+VAAE P +L++SG+TG+PK + T L AA
Sbjct: 322 KDLSWNNFLGNARNLRGVEYVAAEKPAGAYTNILFSSGTTGEPKAIPWTNISPLKSAADA 381
Query: 310 FRYVFDYHRRDVYWCTADVGWITGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINK 369
+ ++ D R DV ++GW+ G ++VYA L N A ++ G+P P ++ +
Sbjct: 382 WCHL-DVQRGDVVAWPTNLGWMMG-PWLVYASLINGACMGLYNGSPLGPTFAKF---VQD 436
Query: 370 YKVTQFYTAPTAIRALMAHYNVEDGNLTMPDLSSLRVLGSVGEPINPAAWLWYYTRIGKE 429
+V+ P+ +R + N G D S +R GS GE N +LW R +
Sbjct: 437 AEVSVLGVIPSIVRT-WQNSNSTSGY----DWSRIRCFGSTGEASNIDEYLWLMGRAHYK 491
Query: 430 RCSIVDTFWQTETGGHVITG 449
+++ TE GG I+G
Sbjct: 492 --PVIEYCGGTEIGGSFISG 509
Score = 60.5 bits (140), Expect = 4e-09
Identities = 57/182 (31%), Positives = 80/182 (43%), Gaps = 14/182 (7%)
Query: 509 ILNEDGVEIE--GPGEGYLVFARPWPGMMRTLLEDQDRFERVYFSKFPGYYCT-----GD 561
IL+ED I G G L G TLL + F +VYF P + GD
Sbjct: 531 ILDEDSNPIPPYAAGVGELALCPHMFGASSTLLNG-NHF-KVYFQGMPTFQGQILRRHGD 588
Query: 562 GVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLAS-DPRVSEAAVVPKPHEVKG-EA 619
R GY GR DD +N+ G + + E+E V S D V E A + P G E
Sbjct: 589 LFERTSKGYYRAHGRADDTMNLGGIKVGSIEIERVCNSVDDSVLETAAIGVPPPSGGPEQ 648
Query: 620 LYCFVVLKEGLQFDADL---VKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRIL 676
L VV K + DL K+ +++K+ ++ P LP+T + K+MRR+L
Sbjct: 649 LVIAVVFKSPEFSNPDLNLLKKSFNSEIQKKLNPLFKVSSVVTLPSLPRTATNKVMRRVL 708
Query: 677 RK 678
R+
Sbjct: 709 RQ 710
>At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase family
protein similar to peroxisomal-coenzyme A synthetase
(FAT2) [gi:586339] from Saccharomyces cerevisiae;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA; identical to cDNA adenosine
monophosphate binding protein 3 AMPBP3
(AMPBP3)GI:20799714
Length = 514
Score = 84.2 bits (199), Expect = 3e-16
Identities = 59/173 (34%), Positives = 87/173 (50%), Gaps = 8/173 (4%)
Query: 504 GVEPKILNEDGVEIEGPGEGYLVFARPWPGMMRTLLEDQDRFERVYFSKFPGYYCTGDGV 563
G E ILNE G EI+ P V R P + + + + + + +F G++ TGD
Sbjct: 342 GQEMAILNEKG-EIQEPNNKGEVCIRG-PNVTKGYKNNPEANKAGF--EF-GWFHTGDIG 396
Query: 564 RRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVKGEALYCF 623
D DGYL + GRI +++N G +S EV+ VL + P VS+ P E GE + C
Sbjct: 397 YFDTDGYLHLVGRIKELINRGGEKISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCA 456
Query: 624 VVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRIL 676
V+ +EG + +K ++ + AF P + LPKT SGKI RRI+
Sbjct: 457 VIPREGTTVTEEDIKA---FCKKNLAAFKVPKRVFITDNLPKTASGKIQRRIV 506
Score = 45.6 bits (103), Expect = 1e-04
Identities = 28/80 (35%), Positives = 48/80 (60%), Gaps = 1/80 (1%)
Query: 113 ITYRKLLDLVCQFANGLLAK-GIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGF 171
+T+ +L DL+ + A+ L++ GI+ GD VA+ P +E VI LA R A + A +
Sbjct: 31 LTHARLHDLIERAASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAAY 90
Query: 172 SSDALAERLSDAKAKLLVTA 191
+++ LSD+ +KLL+T+
Sbjct: 91 TAEEFEFYLSDSDSKLLLTS 110
Score = 32.7 bits (71), Expect = 0.87
Identities = 43/189 (22%), Positives = 71/189 (37%), Gaps = 13/189 (6%)
Query: 277 EDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTY 336
+D L+TSG+T +PKGV T L + + V+ D + + G
Sbjct: 162 DDGALFLHTSGTTSRPKGVPLTQLN-LASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLA 220
Query: 337 VVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVEDGNL 396
+ + L A +V F +W + KY T +YTA I ++ +
Sbjct: 221 GLLSSL-GAGAAVTLPAAGRF-SATTFWPDMKKYNAT-WYTAVPTIHQIILDRHASHPET 277
Query: 397 TMPDLSSLRVLGSVGEPINPAAWLWYYTRIGKERCSIVDTFWQTETGGHVITG--LPGAT 454
P LR + S + P G +++ + TE H+++ LP
Sbjct: 278 EYP---KLRFIRSCSASLAPVILSRLEEAFG---APVLEAYAMTE-ATHLMSSNPLPEEG 330
Query: 455 PMKPGAAEK 463
P KPG+ K
Sbjct: 331 PHKPGSVGK 339
>At5g16370.1 68418.m01913 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA adenosine monophosphate binding protein 5 AMPBP5
(AMPBP5) GI:20799718
Length = 552
Score = 82.6 bits (195), Expect = 8e-16
Identities = 58/169 (34%), Positives = 84/169 (49%), Gaps = 4/169 (2%)
Query: 512 EDGVEIEGPGEGYLVFARPWPGMMRTLLEDQDRFERVYFSKFPGYYCTGDGVRRDEDGYL 571
E G +E GE +M L+D E+ + G++ TGD DGYL
Sbjct: 377 ESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALKN---GWFYTGDVGVIHSDGYL 433
Query: 572 WITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVKGEALYCFVVLKEGLQ 631
I R D++ G +S+ EVE VL ++P V+E AVV +P GE FV LK GL
Sbjct: 434 EIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDVFWGETPCAFVSLKSGLT 493
Query: 632 FDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRILRKIA 680
V+ + R+K+ + P T+ LPKT +GK+M+ +LR+IA
Sbjct: 494 QRPTEVEMIE-YCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFVLREIA 541
Score = 35.5 bits (78), Expect = 0.12
Identities = 33/119 (27%), Positives = 51/119 (42%), Gaps = 9/119 (7%)
Query: 272 EWVAAE---DPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADV 328
+W+ E DP+ + YTSG+T PKGVVH G + + + + + VY T +
Sbjct: 177 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSL-IDWTVPKNPVYLWTLPI 235
Query: 329 GWITGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMA 387
G +Y + A T+V P + R +I + VT AP + L A
Sbjct: 236 FHANGWSY-PWGIAAVGGTNVCLRKFD-APLIYR---LIRDHGVTHMCGAPVVLNMLSA 289
>At1g75960.1 68414.m08822 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam profile: PF00501 AMP-binding enzyme;
identical to cDNA adenosine monophosphate binding
protein 8 AMPBP8 (AMPBP8) GI:20799724
Length = 544
Score = 81.8 bits (193), Expect = 1e-15
Identities = 50/126 (39%), Positives = 71/126 (56%), Gaps = 1/126 (0%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G++ TGD DGYL I R D++ G +S+ EVE VL ++P V+EAAVV +P E
Sbjct: 417 GWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPDE 476
Query: 615 VKGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRR 674
GE FV LK GL K + + K+ + AP T+ LPKT +GKI++
Sbjct: 477 FWGETPCAFVSLKPGLT-RKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 535
Query: 675 ILRKIA 680
+L++IA
Sbjct: 536 LLKEIA 541
Score = 41.9 bits (94), Expect = 0.001
Identities = 33/110 (30%), Positives = 49/110 (44%), Gaps = 6/110 (5%)
Query: 278 DPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTYV 337
DP+ V YTSG+T PKGVVH G + + + + VY T + G TY
Sbjct: 185 DPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLT-DWAVPKTPVYLWTLPIFHANGWTY- 242
Query: 338 VYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMA 387
+ A T+V + P + + +I + VT Y AP ++ L A
Sbjct: 243 PWGIAAVGGTNVCVRKL-HAPSI---YHLIRDHGVTHMYGAPIVLQILSA 288
>At1g55320.1 68414.m06319 acyl-activating enzyme 18 (AAE18) nearly
identical to acyl-activating enzyme 18 [Arabidopsis
thaliana] GI:29893268; similar to acetyl-CoA synthetase
[SP|P27095] from Methanothrix soehngenii; contains Pfam
AMP-binding enzyme domain PF00501l; identical to cDNA
acyl-activating enzyme 18 (At1g55320) GI: 29893267
Length = 725
Score = 81.0 bits (191), Expect = 3e-15
Identities = 100/448 (22%), Positives = 184/448 (41%), Gaps = 58/448 (12%)
Query: 16 SYLPSIQKYKEMYERSLTESELFWTEVAQEFQ--WHVAPKPGGFMKYNFDITKGRVFVKF 73
SY ++ ++ S+ E++W+ V E +H P+ + K + +
Sbjct: 113 SYKNPLESFELFRRFSVEHPEVYWSFVIDELSLVFHTPPR--------CILNKSKPEGTW 164
Query: 74 MEEAELNVCYN-LLDRNVKNGFGDKIAFFWEGNSPDD--YTRITYRKLLDLVCQFANGLL 130
+ +A LN+ L+ + D +A W DD R+T ++L + V AN +
Sbjct: 165 LPDAVLNIAECCLMPSSHPKKEDDSVAVVWRNEGFDDSPVNRMTIKELREQVMLVANAI- 223
Query: 131 AKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALAERLSDAKAKLLVT 190
+ KGD +A+ MPM ++AVI LA G + + F++ +A RL +KAK + T
Sbjct: 224 SGSFEKGDTIAIDMPMTVDAVIIYLAIILAGCIVVSIADSFAAKEIATRLKISKAKGIFT 283
Query: 191 ADGAWRGERMY-VYKRTCDDAIEKARTFFKHRVDMCMVVNHLDRVTPGGSLKSEIKDLYN 249
D RG R + +Y R + A K ++V L + +L+
Sbjct: 284 QDYILRGGRRFPLYSRVVEAAPSK------------VIV-----------LPASGTELHV 320
Query: 250 WNEETDIWWHEVLEGE------SNICPPEWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYL 303
E D+ W + L N P ++ E + +L++SG+TG+PK + T +
Sbjct: 321 QLREQDVSWMDFLSNAKPHSSGENYYRPIYLPVESVINILFSSGTTGEPKAIPWTQLSPI 380
Query: 304 MYAAATFRYVFDYHRRDVYWCTADVGWITGHTYVVYAPLANAATSVMFEGTPYFPKVDRY 363
A + ++ D Y ++GW+ G T ++++ AT ++ G+P ++
Sbjct: 381 RSACDGWAHL-DVQVGHTYCWPTNLGWVMGPT-LMFSCFLTGATLALYSGSPLGRGFGKF 438
Query: 364 WSIINKYKVTQFYTAPTAIRALMAHYNVEDGNLTMPDLSSLRVLGSVGEPINPAAWLWYY 423
VT T P+ ++ +E N T ++ + GE N LW
Sbjct: 439 -----DAGVTVLGTVPSLVKTWKRTNCMEGLNWT-----KIKFFATTGEASNVDDVLWLS 488
Query: 424 TRIGKERCSIVDTFWQTETGGHVITGLP 451
++ + +++ TE I G P
Sbjct: 489 SKADYK--PVIECCGGTELASSYIIGSP 514
Score = 66.5 bits (155), Expect = 6e-11
Identities = 48/141 (34%), Positives = 69/141 (48%), Gaps = 9/141 (6%)
Query: 546 ERVYFSKFPGYYCT-----GDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVL-AS 599
+ VYF P Y T GD V+R GY + GR DD +N+ G S+ E+E V +
Sbjct: 571 DEVYFKGMPMYKETRLRRHGDIVKRTVGGYYNVQGRADDTMNLGGIKTSSIEIERVCDQA 630
Query: 600 DPRVSE-AAVVPKPHEVKGEALYCFVVLKEGL--QFDADLVKTLTLLVRQKIGAFAAPDT 656
D +SE AAV P E L F VLKEG Q +L + +++ +
Sbjct: 631 DECISETAAVTLTPPNGGPELLVIFAVLKEGFKQQSGEELKMKFSRTIQKDLNPLFKVSF 690
Query: 657 IQNAPGLPKTRSGKIMRRILR 677
++ P P+T S K++RR+LR
Sbjct: 691 VKIVPEFPRTASSKLLRRVLR 711
>At5g16340.1 68418.m01910 AMP-binding protein, putative similar to
AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
adenosine monophosphate binding protein 6 AMPBP6
(AMPBP6) GI:20799720
Length = 550
Score = 79.0 bits (186), Expect = 1e-14
Identities = 58/177 (32%), Positives = 85/177 (48%), Gaps = 6/177 (3%)
Query: 512 EDGVEIEGPGEGYLVFARPWPGMMRTLLEDQDRFERVYFSKFPGYYCTGDGVRRDEDGYL 571
E G+ +E GE +M L+D E+ + G++ TGD DGYL
Sbjct: 377 ESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTEKALKN---GWFYTGDVGVIHSDGYL 433
Query: 572 WITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVKGEALYCFVVLKEGLQ 631
I R D++ G +S+ EVE VL + P V+E AVV +P E GE FV LK G
Sbjct: 434 EIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAVVARPDEFWGETPCAFVSLKNGFS 493
Query: 632 FDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRILRKIA--VGDHEV 686
+ L R+K+ + P T+ LPK+ +GK+ + +LR IA +GD +
Sbjct: 494 -GKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTGKVTKFVLRDIAKKMGDKTI 549
Score = 35.1 bits (77), Expect = 0.16
Identities = 16/33 (48%), Positives = 21/33 (63%), Gaps = 3/33 (9%)
Query: 272 EWVAAE---DPLFVLYTSGSTGKPKGVVHTTAG 301
+W+ E DP+ + YTSG+T PKGVVH G
Sbjct: 177 KWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRG 209
>At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 546
Score = 76.6 bits (180), Expect = 5e-14
Identities = 47/182 (25%), Positives = 96/182 (52%), Gaps = 6/182 (3%)
Query: 505 VEPKILNEDGVEIEGPGEGYLVFARPWPGMMRTLLEDQDRFERVYFSKFPGYYCTGDGVR 564
+E +I++ +I GP + ++ + P +M+ +++ S+ G+ TGD
Sbjct: 371 MEGRIVDPVTGQILGPKQTGELWLKG-PSIMKGYFSNEEATSSTLDSE--GWLRTGDLCY 427
Query: 565 RDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVKGEALYCFV 624
DEDG++++ R+ +++ G+ ++ AE+E +L + P +++AAV+P P + G+ +V
Sbjct: 428 IDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYV 487
Query: 625 VLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRILRKIAVGDH 684
V K G KT+ V +++ + + +PK SGKI+R+ L KIA +
Sbjct: 488 VRKTGSSLSE---KTIMEFVAKQVAPYKRIRKVAFVSSIPKNPSGKILRKDLIKIATSNS 544
Query: 685 EV 686
++
Sbjct: 545 KL 546
Score = 34.7 bits (76), Expect = 0.22
Identities = 35/144 (24%), Positives = 55/144 (38%), Gaps = 7/144 (4%)
Query: 272 EWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWI 331
E V +D +LY+SG+TG KGV+ + + + CT + I
Sbjct: 184 ERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHI 243
Query: 332 TGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNV 391
G LA +T ++ ++ S I KY+ T P L+A N
Sbjct: 244 YGLAAFATGLLAYGSTIIVLSKF----EMHEMMSAIGKYQATSLPLVPP---ILVAMVNG 296
Query: 392 EDGNLTMPDLSSLRVLGSVGEPIN 415
D DLSS+ + G P++
Sbjct: 297 ADQIKAKYDLSSMHTVLCGGAPLS 320
Score = 32.3 bits (70), Expect = 1.2
Identities = 25/79 (31%), Positives = 41/79 (51%), Gaps = 2/79 (2%)
Query: 113 ITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMP-MILEAVICMLACARIGAVHTVVFAGF 171
+T+ +L V A+ L GIRKG V + P IL V+C L+ +GA+ T
Sbjct: 58 LTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVC-LSVMSLGAIITTTNPLN 116
Query: 172 SSDALAERLSDAKAKLLVT 190
+S+ +A+++ D+ L T
Sbjct: 117 TSNEIAKQIKDSNPVLAFT 135
>At1g76290.1 68414.m08860 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 546
Score = 74.5 bits (175), Expect = 2e-13
Identities = 43/127 (33%), Positives = 71/127 (55%), Gaps = 3/127 (2%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+Y + D D DGY+ R D++ G ++ + E+EG+L S P V +A VV +P E
Sbjct: 410 GWYWSRDMGVIDPDGYIQFKDRSQDVITCGGEIVGSKEIEGILYSHPAVYDAGVVGRPDE 469
Query: 615 VKGEALYCFVVLKEGLQF-DADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMR 673
GE++ FV LKEG + + ++++ + K P T+ + +PKT +GKI +
Sbjct: 470 TLGESMCAFVKLKEGAEAREEEIIEFCKRKLGNK-NMKMIPKTVVFS-DVPKTPTGKIRK 527
Query: 674 RILRKIA 680
+LRK+A
Sbjct: 528 NVLRKMA 534
Score = 35.5 bits (78), Expect = 0.12
Identities = 23/74 (31%), Positives = 35/74 (47%), Gaps = 1/74 (1%)
Query: 264 GESNICPPEWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYW 323
G N P V DP+ + +TSG+T PK VV++ G + A A + + VY
Sbjct: 164 GNPNFKPIRPVDECDPIALNFTSGTTSTPKCVVYSHRGAYLNATAV-GVMNEMKPMPVYL 222
Query: 324 CTADVGWITGHTYV 337
CT + +G Y+
Sbjct: 223 CTVPMYHCSGWCYI 236
>At1g21540.1 68414.m02694 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 9 AMPBP9 (AMPBP9) GI:20799726
Length = 550
Score = 70.9 bits (166), Expect = 3e-12
Identities = 44/127 (34%), Positives = 70/127 (55%), Gaps = 1/127 (0%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G++ +GD EDGYL + R D++ G +S+AEVE VL ++P V EAAVV KP +
Sbjct: 420 GWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKPDK 479
Query: 615 VKGEALYCFVVLKEGLQFDADLV-KTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMR 673
+ GE FV LK + + + + + ++ + P + LPKT +GKI +
Sbjct: 480 MWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGKIQK 539
Query: 674 RILRKIA 680
+LR++A
Sbjct: 540 FLLRQMA 546
Score = 37.9 bits (84), Expect = 0.023
Identities = 32/109 (29%), Positives = 49/109 (44%), Gaps = 10/109 (9%)
Query: 279 PLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYH--RRDVYWCTADVGWITGHTY 336
P+ + YTSG+T PKGVV + M ++ + D+H R VY T + G Y
Sbjct: 189 PMILNYTSGTTSSPKGVVLSHRAIFMLTVSS---LLDWHFPNRPVYLWTLPMFHANGWGY 245
Query: 337 VVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRAL 385
+ A AT+V P + + +I+K+ VT AP + L
Sbjct: 246 -TWGTAAVGATNVCTRRVD-APTI---YDLIDKHHVTHMCAAPMVLNML 289
>At1g21530.1 68414.m02693 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 10 AMPBP10 (AMPBP10) GI:20799728
Length = 547
Score = 70.9 bits (166), Expect = 3e-12
Identities = 46/129 (35%), Positives = 70/129 (54%), Gaps = 3/129 (2%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G++ +GD +DGYL I R D++ G +S+AE+E VL ++P V EAAVV KP +
Sbjct: 415 GWFYSGDIGVIHKDGYLEIKDRSKDVIICGGENISSAEIETVLYTNPVVKEAAVVAKPDK 474
Query: 615 VKGEALYCFVVLKEGLQFDADLVKT---LTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKI 671
+ GE FV LK D + T + + K+ + P + LPKT +GKI
Sbjct: 475 MWGETPCAFVSLKCDNNGDGSVPVTEREIREFCKTKLPKYMVPRKVIFQEELPKTSTGKI 534
Query: 672 MRRILRKIA 680
+ +LR++A
Sbjct: 535 QKFLLRQMA 543
Score = 36.3 bits (80), Expect = 0.071
Identities = 34/133 (25%), Positives = 59/133 (44%), Gaps = 13/133 (9%)
Query: 258 WHEVLE-GESNICPPEWVAAE---DPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYV 313
+ E++E G S +W+ + P+ + YTSG+T PKGVV + M ++
Sbjct: 164 YEEIMERGNSRF---KWIRPQTEWQPMVLNYTSGTTSSPKGVVLSHRAIFMLTVSSL-LD 219
Query: 314 FDYHRRDVYWCTADVGWITGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVT 373
+ R VY T + G Y + A AT++ P + +++I+K+ VT
Sbjct: 220 WSVPNRPVYLWTLPMFHANGWGY-TWGTAAVGATNICTRRVD-APTI---YNLIDKHNVT 274
Query: 374 QFYTAPTAIRALM 386
AP + L+
Sbjct: 275 HMCAAPMVLNMLI 287
>At1g77240.1 68414.m08996 AMP-binding protein, putative strong
similarity to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 545
Score = 70.5 bits (165), Expect = 4e-12
Identities = 43/127 (33%), Positives = 68/127 (53%), Gaps = 4/127 (3%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G++ TGD DGYL + R D++ G +S+ E+E VL ++P + EAAVV KP +
Sbjct: 418 GWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKPDK 477
Query: 615 VKGEALYCFVVLKEGLQFDADLV-KTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMR 673
+ GE FV LK D + + + + K+ + P + LPKT +GKI +
Sbjct: 478 MWGETPCAFVSLK---YHDGSVTEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQK 534
Query: 674 RILRKIA 680
+LR++A
Sbjct: 535 FLLRQMA 541
Score = 45.6 bits (103), Expect = 1e-04
Identities = 34/112 (30%), Positives = 52/112 (46%), Gaps = 10/112 (8%)
Query: 279 PLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYH--RRDVYWCTADVGWITGHTY 336
P+ + YTSG+T PKGVVH+ M +T + D+ R VY T + G +Y
Sbjct: 187 PMVLNYTSGTTSSPKGVVHSHRSVFM---STINSLLDWSLPNRPVYLWTLPMFHANGWSY 243
Query: 337 VVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAH 388
+A A A ++ V +++I+KY+VT AP + L H
Sbjct: 244 -TWATAAVGARNICVTRV----DVPTIFNLIDKYQVTHMCAAPMVLNMLTNH 290
>At1g20560.1 68414.m02563 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to adenosine monophosphate binding
protein 1 AMPBP1 (AMPBP1) GI:20799710
Length = 556
Score = 70.1 bits (164), Expect = 5e-12
Identities = 44/123 (35%), Positives = 67/123 (54%), Gaps = 4/123 (3%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G++ +GD + DGY+ + R D++ G +S+ EVE L + P V EAAVV +P E
Sbjct: 421 GWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDE 480
Query: 615 VKGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRR 674
GE FV LK+G + A+ L R ++ + AP +I LPKT +GK+ +
Sbjct: 481 YWGETACAFVKLKDGSKASAE---ELISYCRDRLPHYMAPRSIV-FEDLPKTSTGKVQKF 536
Query: 675 ILR 677
+LR
Sbjct: 537 VLR 539
Score = 35.1 bits (77), Expect = 0.16
Identities = 61/276 (22%), Positives = 111/276 (40%), Gaps = 27/276 (9%)
Query: 112 RITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGF 171
+ T+R+ D + A+ L GI GD V+V P + V GA+ +
Sbjct: 41 KYTWRQTRDRCVRIASALSQLGISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRH 100
Query: 172 SSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNHL 231
S +A L + K++ AD + + + + C+ K +V + +++
Sbjct: 101 DSSLVAVLLRHSGTKVIF-ADHQF----LQIAEGACEILSNKG-----DKVPILVLIP-- 148
Query: 232 DRVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGSTGK 291
+ +T S K +++ + + V G+S+ D + V YTSG+T
Sbjct: 149 EPLTQSVSRKKRSEEMMEYEDV-------VAMGKSDFEVIRPTDECDAISVNYTSGTTSS 201
Query: 292 PKGVVHTTAG-YLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTYVVYAPLANAATSVM 350
PKGVV++ G YL AA + + H Y T + G +++ A T++
Sbjct: 202 PKGVVYSHRGAYLNSLAAVL--LNEMHSSPTYLWTNPMFHCNGWC-LLWGVTAIGGTNIC 258
Query: 351 FEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALM 386
D I+++KVT APT + ++
Sbjct: 259 LRNVTAKAIFDN----ISQHKVTHMGGAPTILNMII 290
>At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
gi:112801 from Petroselinum crispum, GB:AAD40664 from
[Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559
(1991)); contains Pfam AMP-binding enzyme domain PF00501
Length = 542
Score = 69.7 bits (163), Expect = 6e-12
Identities = 40/124 (32%), Positives = 65/124 (52%), Gaps = 5/124 (4%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD D+DG ++I RI +++ G ++ AE+E +L + P V + AVVP P E
Sbjct: 414 GWLHTGDIGYIDDDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDE 473
Query: 615 VKGE-ALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMR 673
GE C V+ + + + D++ V + + + +PK+ SGKIMR
Sbjct: 474 EAGEIPAACVVINPKATEKEEDILN----FVAANVAHYKKVRAVHFVDSIPKSLSGKIMR 529
Query: 674 RILR 677
R+LR
Sbjct: 530 RLLR 533
>At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 2 AMPBP2 (AMPBP2) GI:20799712
Length = 603
Score = 69.3 bits (162), Expect = 8e-12
Identities = 46/125 (36%), Positives = 67/125 (53%), Gaps = 3/125 (2%)
Query: 556 YYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEV 615
++ +GD + DGY+ I R+ D++ G +S+ EVE VL S V EAAVV +P
Sbjct: 476 WFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHH 535
Query: 616 KGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRI 675
G+ FV LKEG FD + + R + + AP TI +PKT +GK+ + +
Sbjct: 536 WGQTPCGFVKLKEG--FDTIKPEEIIGFCRDHLPHYMAPKTIVFG-DIPKTSTGKVQKYL 592
Query: 676 LRKIA 680
LRK A
Sbjct: 593 LRKKA 597
Score = 42.7 bits (96), Expect = 8e-04
Identities = 68/283 (24%), Positives = 115/283 (40%), Gaps = 27/283 (9%)
Query: 114 TYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSS 173
TY++ L L A+ L GI +GD VA P + A G + +
Sbjct: 92 TYQRCLRL----ASALTNLGISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDP 147
Query: 174 DALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNHLDR 233
L+ L+ ++AK+L + + + D + +T ++ + N D
Sbjct: 148 STLSVLLAHSEAKILFVDH-----QLLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDD 202
Query: 234 VTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPP--EWVAAEDPLFVLYTSGSTGK 291
S K +++ ET + + + E I P EW DP+ + YTSG+T +
Sbjct: 203 SDEDSSSTFASKYSFDYEYETLL---KSGDSEFEIIKPRCEW----DPISINYTSGTTSR 255
Query: 292 PKGVVHTTAG-YLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTYVVYAPLANAATSVM 350
PKGVV++ G YL A F + + VY T + G +V+ A T++
Sbjct: 256 PKGVVYSHRGAYLNSLATVFLHQMSVY--PVYLWTVPMFHCNGWC-LVWGVAAQGGTNIC 312
Query: 351 FEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVED 393
PK+ + I +KVT APT + ++ +Y V +
Sbjct: 313 LRKVS--PKM--IFKNIAMHKVTHMGGAPTVLN-MIVNYTVTE 350
>At3g16910.1 68416.m02162 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501; identical to cDNA adenosine monophosphate
binding protein 7 AMPBP7 (AMPBP7) GI:20799722
Length = 569
Score = 68.9 bits (161), Expect = 1e-11
Identities = 44/125 (35%), Positives = 68/125 (54%), Gaps = 3/125 (2%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G++ +GD + D Y+ I R D++ G +S+ EVE V+ P V EA+VV +P E
Sbjct: 432 GWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASVVARPDE 491
Query: 615 VKGEALYCFVVLKEGLQ-FDAD-LVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIM 672
E+ FV LK + D + L + + R+K+ A+ P ++ P LPKT +GKI
Sbjct: 492 RWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGP-LPKTATGKIQ 550
Query: 673 RRILR 677
+ ILR
Sbjct: 551 KHILR 555
Score = 41.5 bits (93), Expect = 0.002
Identities = 37/131 (28%), Positives = 57/131 (43%), Gaps = 9/131 (6%)
Query: 284 YTSGSTGKPKGVV-HTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTYVVYAPL 342
YTSG+T PKGVV H Y+M A + ++ VY T + G + ++
Sbjct: 204 YTSGTTASPKGVVLHHRGAYIM--ALSNPLIWGMQDGAVYLWTLPMFHCNGWCFP-WSLA 260
Query: 343 ANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVEDGNLTMPDLS 402
+ TS+ +S+I KYKVT F AP + A++ + ED L +P
Sbjct: 261 VLSGTSICLRQVT----AKEVYSMIAKYKVTHFCAAPVVLNAIV-NAPKEDTILPLPHTV 315
Query: 403 SLRVLGSVGEP 413
+ G+ P
Sbjct: 316 HVMTAGAAPPP 326
>At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501; acyl-activating
enzyme superfamily; identical to cDNA 4-coumarate-CoA
ligase-like protein (At4g05160) GI:29893226
Length = 544
Score = 68.5 bits (160), Expect = 1e-11
Identities = 50/188 (26%), Positives = 92/188 (48%), Gaps = 8/188 (4%)
Query: 490 LPNKSNRTRGFPFFGVEPKILNEDGVEIEGPGEGYLVFARPWPGMMRTLLEDQDRFERVY 549
L +++ + G GVE +I++ + + + P + ++ R P MM+ L + +
Sbjct: 356 LGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRG-PNMMKGYLNNPQATKETI 414
Query: 550 FSKFPGYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVV 609
K + TGD +EDG L++ RI +++ G ++ AE+EG+L S P + +A V+
Sbjct: 415 DKK--SWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVI 472
Query: 610 PKPHEVKGEALYCFVVLKEGLQF-DADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRS 668
P P E GE FVV + D+ K + +++ + + +PK+ +
Sbjct: 473 PFPDEEAGEVPIAFVVRSPNSSITEQDIQK----FIAKQVAPYKRLRRVSFISLVPKSAA 528
Query: 669 GKIMRRIL 676
GKI+RR L
Sbjct: 529 GKILRREL 536
Score = 47.2 bits (107), Expect = 4e-05
Identities = 21/80 (26%), Positives = 44/80 (55%)
Query: 113 ITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFS 172
+T+ +L V + A+G GIRK D V ++ P + +C LA IG V T ++
Sbjct: 55 LTFSQLKSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYT 114
Query: 173 SDALAERLSDAKAKLLVTAD 192
+ +++++ D+ K++++ +
Sbjct: 115 VNEVSKQIKDSNPKIIISVN 134
Score = 36.3 bits (80), Expect = 0.071
Identities = 35/144 (24%), Positives = 59/144 (40%), Gaps = 14/144 (9%)
Query: 274 VAAEDPLFVLYTSGSTGKPKGVVHTTAGYL---MYAAATFRYVFDYHRRDVYWCTADVGW 330
+ D +LY+SG+TG KGV T ++ + + +YH V+ C +
Sbjct: 187 IKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYH--GVFLCFLPMFH 244
Query: 331 ITGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYN 390
+ G + Y+ L V +++ I K++VT + P AL
Sbjct: 245 VFGLAVITYSQLQRGNALVSMARF----ELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 300
Query: 391 VEDGNLTMPDLSSLRVLGSVGEPI 414
V+ DLSSL+ +GS P+
Sbjct: 301 VKKF-----DLSSLKYIGSGAAPL 319
>At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|Q9S725 from Arabidopsis thaliana and SP|P17814 from
Oryza sativa; contains Pfam AMP-binding enzyme domain
PF00501
Length = 565
Score = 68.5 bits (160), Expect = 1e-11
Identities = 44/175 (25%), Positives = 89/175 (50%), Gaps = 6/175 (3%)
Query: 505 VEPKILNEDGVEIEGPGEGYLVFARPWPGMMRTLLEDQDRFERVYFSKFPGYYCTGDGVR 564
VE KI++ D + G + ++ R P +M+ ++++ S+ G+ TGD
Sbjct: 392 VEGKIVDPDTGRVLGVNQTGELWIRS-PTVMKGYFKNKEATASTIDSE--GWLKTGDLCY 448
Query: 565 RDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVKGEALYCFV 624
D DG++++ R+ +++ +G+ ++ AE+E +L + P +++AAV+P P G+ ++
Sbjct: 449 IDGDGFVFVVDRLKELIKCNGYQVAPAELEALLLAHPEIADAAVIPIPDMKAGQYPMAYI 508
Query: 625 VLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRILRKI 679
V K G + V +++ + + +PK SGKI+RR L K+
Sbjct: 509 VRKVGSNLSESEIMG---FVAKQVSPYKKIRKVTFLASIPKNPSGKILRRELTKL 560
Score = 34.3 bits (75), Expect = 0.29
Identities = 22/79 (27%), Positives = 42/79 (53%)
Query: 112 RITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGF 171
R+++ +L V + A L A G+RKG+ V + P + I L+ +GA+ T
Sbjct: 74 RLSFPELWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPIN 133
Query: 172 SSDALAERLSDAKAKLLVT 190
+SD +++++ D++ L T
Sbjct: 134 TSDEISKQIGDSRPVLAFT 152
Score = 30.3 bits (65), Expect = 4.6
Identities = 35/142 (24%), Positives = 62/142 (43%), Gaps = 10/142 (7%)
Query: 274 VAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITG 333
V +D +LY+SG+TG KGV+ + + A +R F +R + CT + I G
Sbjct: 210 VNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQA-YRARFGLEQRTI--CTIPMCHIFG 266
Query: 334 HTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVED 393
+A T V+ P F + + S + ++ + P + A++ N +
Sbjct: 267 FGGFATGLIALGWTIVVL---PKF-DMAKLLSAVETHRSSYLSLVPPIVVAMVNGANEIN 322
Query: 394 GNLTMPDLSSLRVLGSVGEPIN 415
DLSSL + + G P++
Sbjct: 323 SKY---DLSSLHTVVAGGAPLS 341
>At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL
from Pinus taeda, gi:515503, gi:1143308; contains Pfam
AMP-binding enzyme domain PF00501
Length = 566
Score = 67.7 bits (158), Expect = 3e-11
Identities = 39/121 (32%), Positives = 65/121 (53%), Gaps = 3/121 (2%)
Query: 559 TGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVKGE 618
TGD DEDGYL+I RI +++ G ++ A++E VL S P + +AAV P+E GE
Sbjct: 440 TGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGE 499
Query: 619 ALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRILRK 678
FVV ++ + V + V ++ + + +PK+ +GKI+R+ L++
Sbjct: 500 IPVAFVVRRQETTLSEEDVIS---YVASQVAPYRKVRKVVMVNSIPKSPTGKILRKELKR 556
Query: 679 I 679
I
Sbjct: 557 I 557
Score = 35.5 bits (78), Expect = 0.12
Identities = 30/132 (22%), Positives = 59/132 (44%), Gaps = 8/132 (6%)
Query: 258 WHEVLEGESNICPPEWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRY---VF 314
++ +++ P + +D ++Y+SG+TG KGV+ T + R+ +
Sbjct: 185 FYSIMKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQY 244
Query: 315 DY-HRRDVYWCTADVGWITGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVT 373
+Y +VY + I G + V L+ +T V+ + F D ++I ++K+T
Sbjct: 245 EYPGSSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMK---RFDASD-VVNVIERFKIT 300
Query: 374 QFYTAPTAIRAL 385
F P + AL
Sbjct: 301 HFPVVPPMLMAL 312
Score = 29.9 bits (64), Expect = 6.1
Identities = 19/80 (23%), Positives = 41/80 (51%), Gaps = 1/80 (1%)
Query: 113 ITYRKLLDLVCQFANGLL-AKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGF 171
I++ +L +V A G+ G+R+GD V++ +P + + L+ +GA+ T +
Sbjct: 73 ISHTELQIMVQSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSS 132
Query: 172 SSDALAERLSDAKAKLLVTA 191
S + +++S+ L T+
Sbjct: 133 SLGEIKKQVSECSVGLAFTS 152
>At1g66120.1 68414.m07504 acyl-activating enzyme 11 (AAE11) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 11 (At1g66120) GI:29893230,
acyl-activating enzyme 11 [Arabidopsis thaliana]
GI:29893231
Length = 572
Score = 67.7 bits (158), Expect = 3e-11
Identities = 47/126 (37%), Positives = 62/126 (49%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD DGY+ I R D++ G +S+ EVE VL V EAAVV PH
Sbjct: 420 GWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHP 479
Query: 615 VKGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRR 674
+ GE FVVLK+G + L R+ + F P + LPK +GKI++
Sbjct: 480 LWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILKS 539
Query: 675 ILRKIA 680
LR IA
Sbjct: 540 KLRDIA 545
Score = 42.7 bits (96), Expect = 8e-04
Identities = 59/276 (21%), Positives = 105/276 (38%), Gaps = 24/276 (8%)
Query: 111 TRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAG 170
TR T+ + D C+ A LL+ I + D V++ P + + GAV +
Sbjct: 38 TRFTWPQTYDRCCRLAASLLSLNITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTR 97
Query: 171 FSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNH 230
+ +A L A+ K+L Y + + + T+ +++N
Sbjct: 98 LDAKTIAIILRHAEPKILFVD---------YEFAPLIQEVLRLIPTYQSQPHPRIILINE 148
Query: 231 LDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGSTG 290
+D T S + + + L E T + + DP+ + YTSG+T
Sbjct: 149 IDSTTKPFSKELDYEGLIRKGEPTPSSSASMFRVHNE---------HDPISLNYTSGTTA 199
Query: 291 KPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTYVVYAPLANAATSVM 350
PKGVV + G + A ++ ++ VY T + G T+ ++ A T+V
Sbjct: 200 DPKGVVISHQGAYLSALSSI-IGWEMGIFPVYLWTLPMFHCNGWTH-TWSVAARGGTNVC 257
Query: 351 FEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALM 386
P++ + I + VT PT R L+
Sbjct: 258 IRHVT-APEI---YKNIELHGVTHMSCVPTVFRFLL 289
>At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501
Length = 562
Score = 66.9 bits (156), Expect = 4e-11
Identities = 41/128 (32%), Positives = 67/128 (52%), Gaps = 5/128 (3%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD D + +L+I R+ +++ + + E+E +L S+P V +AAVVP P E
Sbjct: 432 GWLKTGDLCYFDSEDFLYIVDRLKELIKYKAYQVPPVELEQILHSNPDVIDAAVVPFPDE 491
Query: 615 VKGEALYCFVVLKEGLQF-DADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMR 673
GE F+V K G +A ++ V +++ + + +PK +GKI+R
Sbjct: 492 DAGEIPMAFIVRKPGSNLNEAQIID----FVAKQVTPYKKVRRVAFINAIPKNPAGKILR 547
Query: 674 RILRKIAV 681
R L KIAV
Sbjct: 548 RELTKIAV 555
Score = 35.5 bits (78), Expect = 0.12
Identities = 39/145 (26%), Positives = 66/145 (45%), Gaps = 16/145 (11%)
Query: 274 VAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAA----TFRYVFDYHRRDVYWCTADVG 329
V DP +L++SG+TG+ KGV+ T + A T + +Y R ++ + +
Sbjct: 201 VNQSDPAAILFSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQDPVNYDRVGLF--SLPLF 258
Query: 330 WITGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHY 389
+ G ++ A ++ T V+ +++ + + KYKVT +P I AL
Sbjct: 259 HVFGFMMMIRA-ISLGETLVLLGRF----ELEAMFKAVEKYKVTGMPVSPPLIVAL---- 309
Query: 390 NVEDGNLTMPDLSSLRVLGSVGEPI 414
V+ DL SLR LG G P+
Sbjct: 310 -VKSELTKKYDLRSLRSLGCGGAPL 333
>At1g68270.1 68414.m07798 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 535
Score = 66.5 bits (155), Expect = 6e-11
Identities = 47/135 (34%), Positives = 64/135 (47%), Gaps = 7/135 (5%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD DG++ I R D++ G +S+ EVE +L PRV E AVV PH
Sbjct: 390 GWLNTGDVGVIHPDGHIEIKDRSKDIIISGGENISSVEVENILYKHPRVFEVAVVAMPHR 449
Query: 615 VKGEALYCFVVLKEGLQFDAD-------LVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTR 667
V GE F+VL++G D K L R+ + F P + LPK
Sbjct: 450 VWGETPCAFIVLQKGETNKEDDEYKFVAREKELIDYCRENLPHFMCPRKVVFLEELPKNG 509
Query: 668 SGKIMRRILRKIAVG 682
+GKI++ LR I G
Sbjct: 510 NGKILKPNLRAITKG 524
Score = 39.1 bits (87), Expect = 0.010
Identities = 49/191 (25%), Positives = 76/191 (39%), Gaps = 18/191 (9%)
Query: 111 TRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAG 170
TR T+ + D C+ A L++ I K D V+V P A GAV +
Sbjct: 38 TRFTWPQTYDRCCRLAASLISLNIAKNDVVSVVAPNTPAIYEMHFAVPMAGAVLNPINTR 97
Query: 171 FSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNH 230
+ ++ L A+ K+L R ++ +CDD F +D N
Sbjct: 98 LDATSITTILRHAQPKILFIHRNFEPLAREILHLLSCDDLQLNLLVIF---ID---EYNS 151
Query: 231 LDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGSTG 290
RV+ S + + + L E T ++E I +DP+ + YTSG+T
Sbjct: 152 AKRVS---SEELDYESLIQMGEPTS----PLVENMFRI-----QNEQDPISLNYTSGTTA 199
Query: 291 KPKGVVHTTAG 301
PKGVV + G
Sbjct: 200 DPKGVVISHRG 210
>At3g16170.1 68416.m02041 acyl-activating enzyme 13 (AAE13) similar
to malonyl CoA synthetase GB:AAF28840 from
[Bradyrhizobium japonicum]; contains Pfam AMP-binding
enzyme domain PF00501; identical to cDNA acyl-activating
enzyme 13 (At3g16170) GI:29893232, acyl-activating
enzyme 13 [Arabidopsis thaliana] GI:29893233
Length = 544
Score = 64.9 bits (151), Expect = 2e-10
Identities = 63/214 (29%), Positives = 95/214 (44%), Gaps = 22/214 (10%)
Query: 478 KQGWDRTVMIVHLPNKSNR---TRGFPFFGVEPKILNEDGVEIEGPGEGYLVFARPWPGM 534
+ G VM + P + R T G P GVE KI ED + G GE + P
Sbjct: 334 RYGMTEFVMAMSNPLRGARNAGTVGKPLPGVEAKI-KEDENDANGVGE--ICVKSP---- 386
Query: 535 MRTLLEDQDRFERVYFSKFP--GYYCTGDGVRRDEDGYLWITGRID-DMLNVSGHLMSTA 591
+L ++ V F GY+ TGD R DEDGY I GR D++ V G+ +S
Sbjct: 387 --SLFKEYWNLPEVTKESFTEDGYFKTGDAGRVDEDGYYVILGRNSADIMKVGGYKLSAL 444
Query: 592 EVEGVLASDPRVSEAAVVPKPHEVKGEALYCFVV----LKEGLQFDADLVKTLTLL---V 644
E+E L P V+E V+ GEA+ ++ K+ + ++ V TL L
Sbjct: 445 EIESTLLEHPTVAECCVLGLTDNDYGEAVTAIIIAESAAKKRREDESKPVITLEELCGWA 504
Query: 645 RQKIGAFAAPDTIQNAPGLPKTRSGKIMRRILRK 678
+ K+ + P + LP+ GK+ ++ L+K
Sbjct: 505 KDKLAPYKLPTRLLIWESLPRNAMGKVNKKELKK 538
Score = 42.7 bits (96), Expect = 8e-04
Identities = 16/22 (72%), Positives = 20/22 (90%)
Query: 277 EDPLFVLYTSGSTGKPKGVVHT 298
+DP ++YTSG+TGKPKGVVHT
Sbjct: 170 DDPALIVYTSGTTGKPKGVVHT 191
>At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2
[gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis
thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum
Length = 570
Score = 62.9 bits (146), Expect = 7e-10
Identities = 39/123 (31%), Positives = 64/123 (52%), Gaps = 3/123 (2%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD D+D ++I R+ +++ G+ ++ AE+E +L S P + +AAVV E
Sbjct: 442 GWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDE 501
Query: 615 VKGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRR 674
V E FV +G Q D VK+ V +++ + + +PK SGKI+R+
Sbjct: 502 VADEVPVAFVARSQGSQLTEDDVKS---YVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 558
Query: 675 ILR 677
LR
Sbjct: 559 DLR 561
Score = 51.2 bits (117), Expect = 2e-06
Identities = 73/299 (24%), Positives = 126/299 (42%), Gaps = 42/299 (14%)
Query: 113 ITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFS 172
+TY + + + A G+ GIR GD V + +P E + LA A +GAV T ++
Sbjct: 74 LTYADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYT 133
Query: 173 SDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNHLD 232
+A++ + AK+++T K+ D + + D ++V LD
Sbjct: 134 QPEIAKQAKASAAKMIIT-------------KKCLVDKLTNLKN------DGVLIV-CLD 173
Query: 233 RVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGSTGKP 292
G + S ++ E T E+ + P+ ++ ED + + Y+SG+TG P
Sbjct: 174 DDGDNGVVSSSDDGCVSFTELTQ-------ADETELLKPK-ISPEDTVAMPYSSGTTGLP 225
Query: 293 KGVVHTTAGYLMYAAATF---RYVFDYHRRDVYWCTADVGWITGHTYVVYAPLANA-ATS 348
KGV+ T G + A ++ DV C + H Y + A + +A T
Sbjct: 226 KGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCFLPM----FHIYALDALMLSAMRTG 281
Query: 349 VMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVEDGNLTMPDLSSLRVL 407
P F +++ +I +YKVT AP + A + E DLSS+R++
Sbjct: 282 AALLIVPRF-ELNLVMELIQRYKVTVVPVAPPVVLAFIKSPETE-----RYDLSSVRIM 334
>At1g65890.1 68414.m07477 acyl-activating enzyme 12 (AAE12) similar
to AMP-binding protein GI:1903034 from [Brassica napus];
contains Pfam AMP-binding domain PF00501; identical to
cDNA acyl-activating enzyme 12 (At1g65890) mRNA
GI:29893228, acyl-activating enzyme 12 [Arabidopsis
thaliana] GI:29893229
Length = 578
Score = 62.9 bits (146), Expect = 7e-10
Identities = 46/135 (34%), Positives = 67/135 (49%), Gaps = 7/135 (5%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ +GD DG++ I R D++ G +S+ EVE ++ P+V E AVV PH
Sbjct: 420 GWLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHP 479
Query: 615 VKGEALYCFVVLKEGLQFDAD----LV---KTLTLLVRQKIGAFAAPDTIQNAPGLPKTR 667
GE FVVL++G + D LV + L R+ + F P + LPK
Sbjct: 480 TWGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNG 539
Query: 668 SGKIMRRILRKIAVG 682
+GKI++ LR IA G
Sbjct: 540 NGKILKPKLRDIAKG 554
Score = 45.6 bits (103), Expect = 1e-04
Identities = 80/338 (23%), Positives = 131/338 (38%), Gaps = 35/338 (10%)
Query: 111 TRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAG 170
TR T+ + D C+ A L++ I K D V+V P A GAV +
Sbjct: 38 TRFTWPQTYDRCCRLAASLISLNIGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTR 97
Query: 171 FSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNH 230
+ ++A L AK K+L R + + +D+ F H +D V+
Sbjct: 98 LDATSIAAILRHAKPKILFIYRSFEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVS- 156
Query: 231 LDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGSTG 290
S +S+ + L E T + + C + DP+ + YTSG+T
Sbjct: 157 --------SEESDYECLIQRGEPTPLLLARMF------CIQD---EHDPISLNYTSGTTA 199
Query: 291 KPKGVVHTTAG-YLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTYVVYAPLANAATSV 349
PKGVV + G YL +A + + VY T + G T+ + A TSV
Sbjct: 200 DPKGVVISHRGAYLSTLSAIIGW--EMGTCPVYLWTLPMFHCNGWTF-TWGTAARGGTSV 256
Query: 350 MFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVEDGNLTMPDLSSLRVLGS 409
P++ + I + VT PT L+ +++ + + P + VL
Sbjct: 257 CMRHVT-APEI---YKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRSGP----VHVLTG 308
Query: 410 VGEPINPAAWLWYYTRIGKERCSIVDTFWQTETGGHVI 447
P PAA + R+G + ++ + TE G V+
Sbjct: 309 GSPP--PAALVKKVQRLGFQ---VMHAYGLTEATGPVL 341
>At1g77590.1 68414.m09034 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS9) similar to LACS 3 [SP|O95573] from Homo Sapiens,
LACS 3 [SP|Q63151] from Rattus norvegicus; contains Pfam
HMM hit: AMP-binding enzymes PF00501
Length = 691
Score = 62.5 bits (145), Expect = 9e-10
Identities = 57/214 (26%), Positives = 98/214 (45%), Gaps = 26/214 (12%)
Query: 109 DYTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVF 168
DY +T+ K L+ VC FA+GL+ G + +RVA++ E I + C R ++
Sbjct: 103 DYEWLTFGKTLEAVCDFASGLVQIGHKTEERVAIFADTREEWFISLQGCFRRNVTVVTIY 162
Query: 169 AGFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVV 228
+ +AL L++ + ++ G+ +++ D ++ T RV +CM
Sbjct: 163 SSLGEEALCHSLNETEVTTVIC--GSKELKKLM-------DISQQLETV--KRV-ICM-- 208
Query: 229 NHLDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGS 288
D P S++ + TD+ + L E+ + P + + D ++YTSGS
Sbjct: 209 ---DDEFP-----SDVNSNWMATSFTDV---QKLGRENPV-DPNFPLSADVAVIMYTSGS 256
Query: 289 TGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVY 322
TG PKGV+ T L +A V D +RD+Y
Sbjct: 257 TGLPKGVMMTHGNVLATVSAVMTIVPDLGKRDIY 290
>At1g65880.1 68414.m07476 AMP-dependent synthetase and ligase family
protein similar to AMP-binding protein GI:1903034 from
[Brassica napus]; contains Pfam AMP-binding domain
PF00501
Length = 580
Score = 62.5 bits (145), Expect = 9e-10
Identities = 50/147 (34%), Positives = 68/147 (46%), Gaps = 8/147 (5%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD DG++ I R D++ G +S+ EVE VL P+V E AVV PH
Sbjct: 420 GWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHP 479
Query: 615 VKGEALYCFVVL-KEGLQFDADLV-------KTLTLLVRQKIGAFAAPDTIQNAPGLPKT 666
GE FVVL K D V + L R+ + F P + LPK
Sbjct: 480 TWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKN 539
Query: 667 RSGKIMRRILRKIAVGDHEVGDISTLA 693
+GKI++ LR IA G +I+ +A
Sbjct: 540 GNGKILKPKLRDIAKGLVVEDEINVIA 566
Score = 37.9 bits (84), Expect = 0.023
Identities = 45/171 (26%), Positives = 72/171 (42%), Gaps = 17/171 (9%)
Query: 278 DPLFVLYTSGSTGKPKGVVHTTAG-YLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTY 336
DP+ + YTSG+T PKGVV + G YL +A + + VY T + G T+
Sbjct: 187 DPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGW--EMGTCPVYLWTLPMFHCNGWTF 244
Query: 337 VVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVEDGNL 396
+ A TSV P++ + I + VT PT L+ +++
Sbjct: 245 -TWGTAARGGTSVCMRHVT-APEI---YKNIEMHNVTHMCCVPTVFNILLKGNSLD---- 295
Query: 397 TMPDLSSLRVLGSVGEPINPAAWLWYYTRIGKERCSIVDTFWQTETGGHVI 447
P + VL P PAA + R+G + ++ + QTE G ++
Sbjct: 296 LSPRSGPVHVLTGGSPP--PAALVKKVQRLGFQ---VMHAYGQTEATGPIL 341
Score = 34.3 bits (75), Expect = 0.29
Identities = 23/78 (29%), Positives = 34/78 (43%)
Query: 111 TRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAG 170
TR T+ + D C+ A L++ I K D V+V P A GAV +
Sbjct: 38 TRFTWPQTYDRCCRLAASLISLNISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTR 97
Query: 171 FSSDALAERLSDAKAKLL 188
+ ++A L AK K+L
Sbjct: 98 LDATSIAAILRHAKPKIL 115
>At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2,
Arabidopsis thaliana [gi:12229665], 4CL1, Nicotiana
tabacum [gi:12229631]; contains Pfam AMP-binding enzyme
domain PF00501
Length = 550
Score = 62.1 bits (144), Expect = 1e-09
Identities = 36/128 (28%), Positives = 68/128 (53%), Gaps = 5/128 (3%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD D DG+L+I R+ +++ G+ + AE+E +L + P + +AAV+P P +
Sbjct: 424 GWLKTGDLCYIDNDGFLFIVDRLKELIKYKGYQVPPAELEALLLNHPDILDAAVIPFPDK 483
Query: 615 VKGEALYCFVVLKEGLQFDADLV-KTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMR 673
G+ +V K +++L K + + +++ + + +PKT SGK +R
Sbjct: 484 EAGQFPMAYVARKP----ESNLCEKKVIDFISKQVAPYKKIRKVAFIDSIPKTPSGKTLR 539
Query: 674 RILRKIAV 681
+ L K A+
Sbjct: 540 KDLIKFAI 547
Score = 41.9 bits (94), Expect = 0.001
Identities = 37/142 (26%), Positives = 60/142 (42%), Gaps = 8/142 (5%)
Query: 274 VAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITG 333
V +D +LY+SG+TG+ KGV + + + A F+ + + CT + G
Sbjct: 196 VHKDDTAMLLYSSGTTGRSKGVNSSHGNLIAHVARYIAEPFE-QPQQTFICTVPLFHTFG 254
Query: 334 HTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVED 393
V A LA T V+ P F + + + KY+ T P L+ N D
Sbjct: 255 LLNFVLATLALGTTVVIL---PRF-DLGEMMAAVEKYRATTLILVPP---VLVTMINKAD 307
Query: 394 GNLTMPDLSSLRVLGSVGEPIN 415
+ D+S LR + G P++
Sbjct: 308 QIMKKYDVSFLRTVRCGGAPLS 329
Score = 34.3 bits (75), Expect = 0.29
Identities = 25/80 (31%), Positives = 40/80 (50%), Gaps = 1/80 (1%)
Query: 112 RITYRKLLDLVCQFANGLLAK-GIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAG 170
RI++ L V + A+ LL GIR+GD V V P + I L+ +GAV T
Sbjct: 64 RISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTANPL 123
Query: 171 FSSDALAERLSDAKAKLLVT 190
++ + +++D+ KL T
Sbjct: 124 NTASEILRQIADSNPKLAFT 143
>At1g51680.1 68414.m05822 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 561
Score = 62.1 bits (144), Expect = 1e-09
Identities = 40/129 (31%), Positives = 66/129 (51%), Gaps = 4/129 (3%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD D+D L+I R+ +++ G ++ AE+E +L P +++ AVV E
Sbjct: 435 GWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEE 494
Query: 615 VKGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRR 674
GE FVV + + D VK V +++ + + + +PK SGKI+R+
Sbjct: 495 AAGEVPVAFVVKSKDSELSEDDVKQ---FVSKQVVFYKRINKVFFTESIPKAPSGKILRK 551
Query: 675 ILR-KIAVG 682
LR K+A G
Sbjct: 552 DLRAKLANG 560
Score = 35.9 bits (79), Expect = 0.093
Identities = 39/145 (26%), Positives = 63/145 (43%), Gaps = 14/145 (9%)
Query: 274 VAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATF---RYVFDYHRRDVYWCTADVGW 330
++ +D + + Y+SG+TG PKGV+ T G + A +H DV C +
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258
Query: 331 ITGHTYVVYAPL-ANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHY 389
I ++ L AA +M P F +++ +I + KVT P + A+
Sbjct: 259 IYALNSIMLCGLRVGAAILIM----PKF-EINLLLELIQRCKVTVAPMVPPIVLAIAKSS 313
Query: 390 NVEDGNLTMPDLSSLRVLGSVGEPI 414
E DLSS+RV+ S P+
Sbjct: 314 ETE-----KYDLSSIRVVKSGAAPL 333
>At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 561
Score = 60.9 bits (141), Expect = 3e-09
Identities = 38/123 (30%), Positives = 64/123 (52%), Gaps = 3/123 (2%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD DED ++I R+ +++ G + AE+E +L + +++AAVVP+ E
Sbjct: 438 GWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDE 497
Query: 615 VKGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRR 674
V GE FVV G + VK V +++ + + +PK+ SGKI+R+
Sbjct: 498 VAGEVPVAFVVRSNGNDITEEDVKE---YVAKQVVFYKRLHKVFFVASIPKSPSGKILRK 554
Query: 675 ILR 677
L+
Sbjct: 555 DLK 557
Score = 46.4 bits (105), Expect = 7e-05
Identities = 25/71 (35%), Positives = 40/71 (56%), Gaps = 1/71 (1%)
Query: 121 LVCQ-FANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALAER 179
L+C+ A+GL GIRKGD + + + E V + + IGAV T ++S L ++
Sbjct: 83 LICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQ 142
Query: 180 LSDAKAKLLVT 190
L + AKL++T
Sbjct: 143 LKSSGAKLIIT 153
>At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 /
4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725
4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
(4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana}
Length = 556
Score = 59.7 bits (138), Expect = 7e-09
Identities = 36/123 (29%), Positives = 61/123 (49%), Gaps = 3/123 (2%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
G+ TGD D+D L+I R+ +++ G ++ AE+E +L P +++ AVV E
Sbjct: 428 GWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEE 487
Query: 615 VKGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRR 674
GE FVV + D +K V +++ + + + +PK SGKI+R+
Sbjct: 488 DAGEVPVAFVVRSKDSNISEDEIKQ---FVSKQVVFYKRINKVFFTDSIPKAPSGKILRK 544
Query: 675 ILR 677
LR
Sbjct: 545 DLR 547
Score = 37.5 bits (83), Expect = 0.031
Identities = 39/147 (26%), Positives = 64/147 (43%), Gaps = 12/147 (8%)
Query: 271 PEWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATF---RYVFDYHRRDVYWCTAD 327
PE ++ ED + + ++SG+TG PKGV+ T G + A ++R DV C
Sbjct: 189 PEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLP 248
Query: 328 VGWITGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMA 387
+ I ++ L AT ++ P F ++ I + KVT P + A+
Sbjct: 249 MFHIYALNSIMLCSLRVGATILIM---PKF-EITLLLEQIQRCKVTVAMVVPPIVLAIAK 304
Query: 388 HYNVEDGNLTMPDLSSLRVLGSVGEPI 414
E DLSS+R++ S P+
Sbjct: 305 SPETE-----KYDLSSVRMVKSGAAPL 326
Score = 37.1 bits (82), Expect = 0.040
Identities = 21/67 (31%), Positives = 37/67 (55%)
Query: 124 QFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALAERLSDA 183
+ A GL G+++ D V + +P E V+ LA + IGA+ T F+ ++++ +
Sbjct: 75 KLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKAS 134
Query: 184 KAKLLVT 190
AKL+VT
Sbjct: 135 AAKLIVT 141
>At5g35930.1 68418.m04322 AMP-dependent synthetase and ligase family
protein similar to iturin A synthetase C [Bacillus
subtilis] GI:16040972; contains Pfam profile PF00501:
AMP-binding enzyme
Length = 1040
Score = 52.0 bits (119), Expect = 1e-06
Identities = 35/124 (28%), Positives = 58/124 (46%)
Query: 556 YYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEV 615
YY TGD R+ G L GR D + ++G M+ E+E L +P ++EA V+ E
Sbjct: 352 YYRTGDYGRQLSSGDLIFIGRRDRTVKLNGKRMALEEIETTLELNPDIAEAVVLLSRDET 411
Query: 616 KGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRI 675
+ +L FVVL + ++ ++ + K+ P+ LP T SGK+
Sbjct: 412 ELASLKAFVVLNKESNSSDGIIFSIRNWMGGKLPPVMIPNHFVLVEKLPLTSSGKVDYEA 471
Query: 676 LRKI 679
L ++
Sbjct: 472 LARL 475
Score = 43.2 bits (97), Expect = 6e-04
Identities = 39/164 (23%), Positives = 69/164 (42%), Gaps = 6/164 (3%)
Query: 281 FVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTYVVYA 340
+++YTSGSTGKPKGV T G L+ + ++ + VG+I H
Sbjct: 106 YLMYTSGSTGKPKGVCGTEQG-LLNRFLWMQELYPVVGEQRFAFKTSVGFI-DHIQEFLG 163
Query: 341 PLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVEDGNLTMPD 400
+ ++ V+ T + + +Y +++ P+ IRA++ N +
Sbjct: 164 AILSSTALVIPPFTLLKENMISIIDFLEEYSISRLLAVPSMIRAILPTLQHRGHNNKLQS 223
Query: 401 LSSLRVLGSVGEPINPAAWLWYYTRIGKERCSIVDTFWQTETGG 444
L VL GEP + W ++ + E C ++ + TE G
Sbjct: 224 CLKLVVLS--GEPFPVSLWDSLHSLL-PETC-FLNLYGSTEVSG 263
>At2g04350.2 68415.m00434 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 52.0 bits (119), Expect = 1e-06
Identities = 50/215 (23%), Positives = 87/215 (40%), Gaps = 22/215 (10%)
Query: 109 DYTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVF 168
+Y +Y ++ + VC FA+GL+ G DRVA++ E I C R ++
Sbjct: 126 EYKWQSYGEVFERVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIY 185
Query: 169 AGFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVV 228
A +AL L++ + L+ CD K + + + +
Sbjct: 186 ASLGEEALIYSLNETRVSTLI-----------------CDSKQLKKLSAIQSSLKTVKNI 228
Query: 229 NHLDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLE-GESNICPPEWVAAEDPLFVLYTSG 287
+++ G + S D+ + + T EV + G+ N P + +++TSG
Sbjct: 229 IYIEE--DGVDVAS--SDVNSMGDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSG 284
Query: 288 STGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVY 322
STG PKGV+ T + AA + V + D Y
Sbjct: 285 STGLPKGVMITHGNLVATAAGVMKVVPKLDKNDTY 319
>At2g04350.1 68415.m00433 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
(LACS8) similar to LACS 4 [SP|O35547] from Rattus
norvegicus, LACS 4 [SP|O60488] from Homo sapiens;
contains Pfam HMM hit: AMP-binding enzymes PF00501
Length = 720
Score = 52.0 bits (119), Expect = 1e-06
Identities = 50/215 (23%), Positives = 87/215 (40%), Gaps = 22/215 (10%)
Query: 109 DYTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVF 168
+Y +Y ++ + VC FA+GL+ G DRVA++ E I C R ++
Sbjct: 126 EYKWQSYGEVFERVCNFASGLVNVGHNVDDRVAIFSDTRAEWFIAFQGCFRQSITVVTIY 185
Query: 169 AGFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVV 228
A +AL L++ + L+ CD K + + + +
Sbjct: 186 ASLGEEALIYSLNETRVSTLI-----------------CDSKQLKKLSAIQSSLKTVKNI 228
Query: 229 NHLDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLE-GESNICPPEWVAAEDPLFVLYTSG 287
+++ G + S D+ + + T EV + G+ N P + +++TSG
Sbjct: 229 IYIEE--DGVDVAS--SDVNSMGDITVSSISEVEKLGQKNAVQPILPSKNGVAVIMFTSG 284
Query: 288 STGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVY 322
STG PKGV+ T + AA + V + D Y
Sbjct: 285 STGLPKGVMITHGNLVATAAGVMKVVPKLDKNDTY 319
>At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|P14912 and SP|P14913 from Petroselinum crispum;
contains Pfam AMP-binding enzyme domain PF00501
Length = 473
Score = 50.8 bits (116), Expect = 3e-06
Identities = 27/106 (25%), Positives = 61/106 (57%), Gaps = 3/106 (2%)
Query: 505 VEPKILNEDGVEIEGPGEGYLVFARPWPGMMRTLLEDQDRFERVYFSKFPGYYCTGDGVR 564
+E +I++ +I GP + ++ + P +M+ +++ S+ G+ TGD
Sbjct: 371 MEGRIVDPVTGQILGPKQTGELWLKG-PSIMKGYFSNEEATSSTLDSE--GWLRTGDLCY 427
Query: 565 RDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVP 610
DEDG++++ R+ +++ G+ ++ AE+E +L + P +++AAV+P
Sbjct: 428 IDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIP 473
Score = 34.7 bits (76), Expect = 0.22
Identities = 35/144 (24%), Positives = 55/144 (38%), Gaps = 7/144 (4%)
Query: 272 EWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWI 331
E V +D +LY+SG+TG KGV+ + + + CT + I
Sbjct: 184 ERVDQDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHI 243
Query: 332 TGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNV 391
G LA +T ++ ++ S I KY+ T P L+A N
Sbjct: 244 YGLAAFATGLLAYGSTIIVLSKF----EMHEMMSAIGKYQATSLPLVPP---ILVAMVNG 296
Query: 392 EDGNLTMPDLSSLRVLGSVGEPIN 415
D DLSS+ + G P++
Sbjct: 297 ADQIKAKYDLSSMHTVLCGGAPLS 320
Score = 32.3 bits (70), Expect = 1.2
Identities = 25/79 (31%), Positives = 41/79 (51%), Gaps = 2/79 (2%)
Query: 113 ITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMP-MILEAVICMLACARIGAVHTVVFAGF 171
+T+ +L V A+ L GIRKG V + P IL V+C L+ +GA+ T
Sbjct: 58 LTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVC-LSVMSLGAIITTTNPLN 116
Query: 172 SSDALAERLSDAKAKLLVT 190
+S+ +A+++ D+ L T
Sbjct: 117 TSNEIAKQIKDSNPVLAFT 135
>At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase family
protein similar to SP|Q42524 and SP|Q9S725; contains
Pfam AMP-binding enzyme domain PF00501
Length = 447
Score = 48.0 bits (109), Expect = 2e-05
Identities = 41/143 (28%), Positives = 64/143 (44%), Gaps = 11/143 (7%)
Query: 274 VAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRD-VYWCTADVGWIT 332
V +D +LY+SG+TG KGV+ + Y A +Y+ D +RD ++ CT +
Sbjct: 196 VNQDDTAMMLYSSGTTGTSKGVISSHRNLTAYVA---KYIDDKWKRDEIFVCTVPMFHSF 252
Query: 333 GHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVE 392
G +A+ +T V+ +D + KYK T AP L+A N
Sbjct: 253 GLLAFAMGSVASGSTVVILRRF----GLDDMMQAVEKYKATILSLAPP---VLVAMINGA 305
Query: 393 DGNLTMPDLSSLRVLGSVGEPIN 415
D DL+SLR + G P++
Sbjct: 306 DQLKAKYDLTSLRKVRCGGAPLS 328
Score = 31.5 bits (68), Expect = 2.0
Identities = 21/80 (26%), Positives = 42/80 (52%), Gaps = 1/80 (1%)
Query: 112 RITYRKLLDLVCQFANGLLAK-GIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAG 170
R+T+ L +V + A+ L + GIR+GD V + P + + L+ +GAV T
Sbjct: 63 RLTFSDLWRVVDRVADCLYHEVGIRRGDVVLILSPNSIYIPVVCLSVMSLGAVVTTANTL 122
Query: 171 FSSDALAERLSDAKAKLLVT 190
+S ++++++ + L+ T
Sbjct: 123 NTSGEISKQIAQSNPTLVFT 142
>At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 495
Score = 46.4 bits (105), Expect = 7e-05
Identities = 25/71 (35%), Positives = 40/71 (56%), Gaps = 1/71 (1%)
Query: 121 LVCQ-FANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALAER 179
L+C+ A+GL GIRKGD + + + E V + + IGAV T ++S L ++
Sbjct: 83 LICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQ 142
Query: 180 LSDAKAKLLVT 190
L + AKL++T
Sbjct: 143 LKSSGAKLIIT 153
>At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to
acyl-CoA synthetase (MF7P) from Brassica napus
[gi:1617270]
Length = 666
Score = 42.3 bits (95), Expect = 0.001
Identities = 42/205 (20%), Positives = 83/205 (40%), Gaps = 21/205 (10%)
Query: 107 PDDYTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTV 166
P Y TY+++ D+V + N L + G++ + +Y E +I M AC G
Sbjct: 74 PGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGANSPEWIISMEACNAHGLYCVP 133
Query: 167 VFAGFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCM 226
++ +DA+ +S ++ ++ + +++ +TC ++ E +T
Sbjct: 134 LYDTLGADAVEFIISHSEVSIVFVEE-----KKISELFKTCPNSTEYMKTV--------- 179
Query: 227 VVNHLDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTS 286
V+ GG + + ++ + W + GE D ++YTS
Sbjct: 180 -------VSFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYDLPIKKKSDICTIMYTS 232
Query: 287 GSTGKPKGVVHTTAGYLMYAAATFR 311
G+TG PKGV+ + + A R
Sbjct: 233 GTTGDPKGVMISNESIVTLIAGVIR 257
>At2g47240.1 68415.m05899 long-chain-fatty-acid--CoA ligase family
protein / long-chain acyl-CoA synthetase family protein
similar to GI:1617270 (MF7P) and gi:1617628 (MF45P) from
[Brassica napus] ; contains Pfam AMP-binding enzyme
domain PF00501
Length = 660
Score = 42.3 bits (95), Expect = 0.001
Identities = 52/226 (23%), Positives = 92/226 (40%), Gaps = 25/226 (11%)
Query: 76 EAELNVCYNLLDRNVKNGFGDKIAFFWE---GNSPDDYTRITYRKLLDLVCQFANGLLAK 132
++E+ +++ ++V+ F D W Y TY+++ + V Q + L A
Sbjct: 38 DSEITTAWDIFSKSVEK-FPDNNMLGWRRIVDEKVGPYMWKTYKEVYEEVLQIGSALRAA 96
Query: 133 GIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALAERLSDAKAKLLVTAD 192
G G RV +Y + +I M ACA + ++ S A+ + A+ + D
Sbjct: 97 GAEPGSRVGIYGVNCPQWIIAMEACAAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQD 156
Query: 193 GAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNHLDRVTPGGSLKSEIKDLYNWNE 252
+G + + C K K V V + L +K+ Y+W +
Sbjct: 157 TKIKG----LLEPDC-----KCAKRLKAIVSFTNVSDELSHKASEIGVKT-----YSWID 202
Query: 253 ETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGSTGKPKGVVHT 298
+ H E + PP+ A + ++YTSG++G PKGVV T
Sbjct: 203 ----FLHMGREKPEDTNPPK---AFNICTIMYTSGTSGDPKGVVLT 241
>At1g20500.1 68414.m02554 4-coumarate--CoA ligase family /
4-coumaroyl-CoA synthase family similar to SP|Q42524 and
SP|Q9S725; contains Pfam AMP-binding enzyme domain
PF00501
Length = 412
Score = 42.3 bits (95), Expect = 0.001
Identities = 38/143 (26%), Positives = 63/143 (44%), Gaps = 11/143 (7%)
Query: 274 VAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRR-DVYWCTADVGWIT 332
V +D +LY+SG+TG KGV+ + + A R++ D +R D++ CT +
Sbjct: 194 VNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVA---RFISDNLKRDDIFICTVPMFHTY 250
Query: 333 GHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVE 392
G +A +T V+ +D + K++ T AP L+A N
Sbjct: 251 GLLTFAMGTVALGSTVVILRRFQLHDMMD----AVEKHRATALALAPP---VLVAMINDA 303
Query: 393 DGNLTMPDLSSLRVLGSVGEPIN 415
D DLSSL+ + G P++
Sbjct: 304 DLIKAKYDLSSLKTVRCGGAPLS 326
Score = 31.5 bits (68), Expect = 2.0
Identities = 21/79 (26%), Positives = 41/79 (51%), Gaps = 1/79 (1%)
Query: 113 ITYRKLLDLVCQFANGLLAK-GIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGF 171
+T+ L V + A+ L + GIR+GD V + P + + L+ +GAV T
Sbjct: 64 LTFSDLWRAVDRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLN 123
Query: 172 SSDALAERLSDAKAKLLVT 190
+S ++++++D+ L+ T
Sbjct: 124 TSGEISKQIADSNPTLVFT 142
>At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to acyl-CoA synthetase (MF7P) gi:1617270 from
Brassica napus
Length = 666
Score = 41.5 bits (93), Expect = 0.002
Identities = 44/188 (23%), Positives = 79/188 (42%), Gaps = 23/188 (12%)
Query: 110 YTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFA 169
Y TY+++ D+V + N L + GI++G++ +Y E +I M AC G ++
Sbjct: 77 YVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGINCCEWIISMEACNAHGLYCVPLYD 136
Query: 170 GFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVN 229
+ A+ +S A+ + + +++ +TC ++ + +T
Sbjct: 137 TLGAGAVEFIISHAEVSIAFVEE-----KKIPELFKTCPNSTKYMKT------------- 178
Query: 230 HLDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLE-GESNICPPEWVAAEDPLFVLYTSGS 288
V G +K E K+ W E L+ GE D ++YTSG+
Sbjct: 179 ----VVSFGGVKPEQKEEAEKLGLVIHSWDEFLKLGEGKQYELPIKKPSDICTIMYTSGT 234
Query: 289 TGKPKGVV 296
TG PKGV+
Sbjct: 235 TGDPKGVM 242
>At1g64400.1 68414.m07299 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to GI:1617270 (MF7P) from [Brassica napus]
Length = 665
Score = 41.5 bits (93), Expect = 0.002
Identities = 44/189 (23%), Positives = 83/189 (43%), Gaps = 21/189 (11%)
Query: 110 YTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFA 169
Y TY+++ ++V + N + G+ KGD+ +Y E +I M AC G ++
Sbjct: 77 YVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEWIISMEACNAHGLYCVPLYD 136
Query: 170 GFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVN 229
+ A+ + A+ L + ++ +T K+ + K+ V V N
Sbjct: 137 TLGAGAIEFIICHAEVSLAFAEE-----NKISELLKTA----PKSTKYLKYIVSFGEVTN 187
Query: 230 HLDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGST 289
+ RV + +Y+W++ + ++ EG+ P + D ++YTSG+T
Sbjct: 188 N-QRV----EAERHRLTIYSWDQ-----FLKLGEGKHYELPEK--RRSDVCTIMYTSGTT 235
Query: 290 GKPKGVVHT 298
G PKGV+ T
Sbjct: 236 GDPKGVLLT 244
>At4g14070.1 68417.m02172 AMP-binding protein, putative similar to
AMP-binding protein [gi:1617272] from Brassica napus;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA acyl-CoA synthetase-like protein
GI:20799730
Length = 727
Score = 41.1 bits (92), Expect = 0.002
Identities = 45/227 (19%), Positives = 95/227 (41%), Gaps = 16/227 (7%)
Query: 88 RNVKNGFGDKIAFFWEGNSPDDYTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMI 147
R+ +GD++A + P ++TY++L + FA GL G++ +++A++
Sbjct: 104 RSSAEKYGDRVALVDPYHDPP--LKLTYKQLEQEILDFAEGLRVLGVKADEKIALFADNS 161
Query: 148 LEAVICMLACARIGAVHTVVFAGFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTC 207
++ GAV+ V + S + L + +++ +V + + + R
Sbjct: 162 CRWLVSDQGIMATGAVNVVRGSRSSVEELLQIYRHSESVAIVVDNPEF-------FNRIA 214
Query: 208 DDAIEKARTFFKHRVDMCMVVNHLDRVTPGGSLKS-EIKDLYNWNEETDIWWHEVLEGES 266
+ KA F + + VT G + ++ N +E+ ++
Sbjct: 215 ESFTSKASLRFL----ILLWGEKSSLVTQGMQIPVYSYAEIINQGQESRAKLSA--SNDT 268
Query: 267 NICPPEWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYV 313
+++ ++D ++YTSG+TG PKGV+ T L +YV
Sbjct: 269 RSYRNQFIDSDDTAAIMYTSGTTGNPKGVMLTHRNLLHQIKHLSKYV 315
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 40.3 bits (90), Expect = 0.004
Identities = 41/191 (21%), Positives = 80/191 (41%), Gaps = 21/191 (10%)
Query: 110 YTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFA 169
YT ITY++ D + + + ++G+ G +Y E +I M AC G + ++
Sbjct: 77 YTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGANCPEWIIAMEACMSQGITYVPLYD 136
Query: 170 GFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVN 229
+A+ ++ A+ L+ + + ++ C ++ +F + V+
Sbjct: 137 SLGVNAVEFIINHAEVSLVFVQEKT--VSSILSCQKGCSSNLKTIVSFGE--------VS 186
Query: 230 HLDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGST 289
T K++ L++WNE + L G + D ++YTSG+T
Sbjct: 187 S----TQKEEAKNQCVSLFSWNEFS-------LMGNLDEANLPRKRKTDICTIMYTSGTT 235
Query: 290 GKPKGVVHTTA 300
G+PKGV+ A
Sbjct: 236 GEPKGVILNNA 246
>At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14)
identical to acyl-activating enzyme 14 [Arabidopsis
thaliana]; similar to SP|Q42524 4-coumarate--CoA ligase
1 (EC 6.2.1.12) (4-coumaroyl-CoA synthase 1)
{Arabidopsis thaliana}; contains Pfam profile PF00501:
AMP-binding enzyme; identical to cDNA acyl-activating
enzyme 14 (At1g30520) GI:29893263
Length = 560
Score = 39.1 bits (87), Expect = 0.010
Identities = 24/70 (34%), Positives = 32/70 (45%)
Query: 559 TGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVKGE 618
TGD DE G LW+ GR + + G + EVE VL P + A V+ GE
Sbjct: 417 TGDIGAFDEFGNLWLIGRSNGRIKTGGENVYPEEVEAVLVEHPGIVSAVVIGVIDTRLGE 476
Query: 619 ALYCFVVLKE 628
+ V L+E
Sbjct: 477 MVVACVRLQE 486
Score = 37.1 bits (82), Expect = 0.040
Identities = 31/114 (27%), Positives = 55/114 (48%), Gaps = 10/114 (8%)
Query: 275 AAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITGH 334
A++D + + +TSG+TG+PKGV + ++ + A + Y DVY T+ + I G
Sbjct: 170 ASDDAVVICFTSGTTGRPKGVTISHLAFITQSLAKIA-IAGYGEDDVYLHTSPLVHIGGL 228
Query: 335 TYVVYAPLANAATSVMFEGTPYFPKVDRYWS--IINKYKVTQFYTAPTAIRALM 386
+ + A L A V+ PK D + ++ + +T F T P + L+
Sbjct: 229 SSAM-AMLMVGACHVL------LPKFDAKTALQVMEQNHITCFITVPAMMADLI 275
>At1g51680.2 68414.m05823 4-coumarate--CoA ligase 1 /
4-coumaroyl-CoA synthase 1 (4CL1) identical to SP|Q42524
4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
(4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}
Length = 490
Score = 37.9 bits (84), Expect = 0.023
Identities = 18/55 (32%), Positives = 32/55 (58%)
Query: 555 GYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVV 609
G+ TGD D+D L+I R+ +++ G ++ AE+E +L P +++ AVV
Sbjct: 435 GWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVV 489
Score = 35.9 bits (79), Expect = 0.093
Identities = 39/145 (26%), Positives = 63/145 (43%), Gaps = 14/145 (9%)
Query: 274 VAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATF---RYVFDYHRRDVYWCTADVGW 330
++ +D + + Y+SG+TG PKGV+ T G + A +H DV C +
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258
Query: 331 ITGHTYVVYAPL-ANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHY 389
I ++ L AA +M P F +++ +I + KVT P + A+
Sbjct: 259 IYALNSIMLCGLRVGAAILIM----PKF-EINLLLELIQRCKVTVAPMVPPIVLAIAKSS 313
Query: 390 NVEDGNLTMPDLSSLRVLGSVGEPI 414
E DLSS+RV+ S P+
Sbjct: 314 ETE-----KYDLSSIRVVKSGAAPL 333
>At3g23790.1 68416.m02990 AMP-binding protein, putative similar to
AMP-binding protein GB:CAA96521 from [Brassica napus]
(Plant Mol. Biol. (1997) 33 (5), 911-922); contains Pfam
AMP-binding enzyme domain PF00501; identical to cDNA
acyl-CoA synthetase-like protein GI:20799732
Length = 722
Score = 37.5 bits (83), Expect = 0.031
Identities = 48/207 (23%), Positives = 90/207 (43%), Gaps = 18/207 (8%)
Query: 94 FGDKIAFFWEGNSPDDYTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVIC 153
+GD++A + P + TYR+L + F GL G++ +++A++ ++
Sbjct: 94 YGDRVAVVDPYHDPP--STFTYRQLEQEILDFVEGLRVVGVKADEKIALFADNSCRWLVA 151
Query: 154 MLACARIGAVHTVVFAGFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEK 213
GAV+ V + S + L + +++ LV + + + R + K
Sbjct: 152 DQGIMATGAVNVVRGSRSSVEELLQIYCHSESVALVVDNPEF-------FNRIAESFSYK 204
Query: 214 ARTFFKHRV--DMCMVVNHLDRVTPGGSLKSEIKDLYNWNEETDIWWHEVLEGESNICPP 271
A F + + +V R TP S +EIK + +E + +S
Sbjct: 205 AAPKFVILLWGEKSSLVT-AGRHTPVYSY-NEIK---KFGQERRAKFAR--SNDSGKYEY 257
Query: 272 EWVAAEDPLFVLYTSGSTGKPKGVVHT 298
E++ +D ++YTSG+TG PKGV+ T
Sbjct: 258 EYIDPDDIATIMYTSGTTGNPKGVMLT 284
>At5g27600.1 68418.m03305 AMP-binding protein, putative similar to
AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, long-chain-fatty-acid--CoA ligase - Brassica
napus, EMBL:Z72152; contains Pfam AMP-binding enzyme
domain PF00501
Length = 700
Score = 36.7 bits (81), Expect = 0.053
Identities = 23/60 (38%), Positives = 31/60 (51%), Gaps = 1/60 (1%)
Query: 263 EGESNICPPEWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVY 322
+G S++ P ED + YTSG+TG PKGVV T G L+ A ++ DVY
Sbjct: 247 QGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVV-LTHGNLIANVAGSSVEAEFFPSDVY 305
>At3g05970.1 68416.m00681 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase (LACS6) strong similarity
to AMP-binding protein (MF39P) gi:1617274 from Brassica
napus, similar to putative long-chain-fatty-acid--CoA
ligase (brain isozyme) GB:P33124 [Rattus norvegicus];
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA AtLACS6 for long-chain acyl-CoA
synthetase GI:22531705
Length = 701
Score = 34.3 bits (75), Expect = 0.29
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Query: 277 EDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVY 322
+D + YTSG+TG PKGVV T A + A + + + DVY
Sbjct: 261 DDVATICYTSGTTGTPKGVVLTHANLIANVAGS-SFSVKFFSSDVY 305
>At3g58200.1 68416.m06489 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 319
Score = 31.9 bits (69), Expect = 1.5
Identities = 16/48 (33%), Positives = 25/48 (52%)
Query: 162 AVHTVVFAGFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDD 209
A+ + F+GF D L ++L + K K G R + M V+K+ C D
Sbjct: 246 ALQYLKFSGFKVDWLEKKLEEVKEKKKEEQIGETRMQEMKVFKQKCSD 293
>At5g15680.1 68418.m01834 expressed protein
Length = 2151
Score = 29.9 bits (64), Expect = 6.1
Identities = 21/97 (21%), Positives = 42/97 (43%), Gaps = 1/97 (1%)
Query: 64 ITKGRVFVKFMEEAELNVCYNLLDRNVKNGFGDKIAFFWEGNSPDDYTRITYRKLLDLVC 123
+ KG + ++ + C + + N+ + + +SP + I R LL LV
Sbjct: 370 LRKGMLTQDVQQDKLVEFCVTIAEHNLDFAMNHMLLELLKQDSPSEAKIIGLRALLALVM 429
Query: 124 QFANGLLAKGIRKGDRVAVYMPMILEAVICML-ACAR 159
++ + I KG + Y+P + A+ +L +C R
Sbjct: 430 SPSSQYVGLEIFKGHGIGHYIPKVKAAIESILRSCHR 466
>At4g35260.1 68417.m05011 isocitrate dehydrogenase subunit 1 / NAD+
isocitrate dehydrogenase subunit 1 nearly identical to
NAD+ dependent isocitrate dehydrogenase subunit 1
[Arabidopsis thaliana] GI:1766046
Length = 367
Score = 29.9 bits (64), Expect = 6.1
Identities = 26/79 (32%), Positives = 39/79 (49%), Gaps = 4/79 (5%)
Query: 624 VVLKEGLQFDADL-VKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRILRKIAVG 682
V LK GL+ V +L + +R+++ FA+ N PGLP TR + ++R+ G
Sbjct: 94 VCLKGGLKTPVGGGVSSLNVQLRKELDLFASLVNCFNLPGLP-TRHENVDIVVIRENTEG 152
Query: 683 DHEVGDISTLADPGVVEEL 701
E + PGVVE L
Sbjct: 153 --EYAGLEHEVVPGVVESL 169
>At3g49260.2 68416.m05384 calmodulin-binding family protein low
similarity to SF16 protein [Helianthus annuus]
GI:560150; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 471
Score = 29.9 bits (64), Expect = 6.1
Identities = 14/42 (33%), Positives = 21/42 (50%)
Query: 440 TETGGHVITGLPGATPMKPGAAEKKHPVYYSSMDTFRPKQGW 481
+ +GG + TG PG P + ++ PV S T K+GW
Sbjct: 426 SSSGGAITTGYPGPRSPNPKSDIRRKPVSPSQSPTGFGKRGW 467
>At3g49260.1 68416.m05383 calmodulin-binding family protein low
similarity to SF16 protein [Helianthus annuus]
GI:560150; contains Pfam profile PF00612: IQ
calmodulin-binding motif
Length = 471
Score = 29.9 bits (64), Expect = 6.1
Identities = 14/42 (33%), Positives = 21/42 (50%)
Query: 440 TETGGHVITGLPGATPMKPGAAEKKHPVYYSSMDTFRPKQGW 481
+ +GG + TG PG P + ++ PV S T K+GW
Sbjct: 426 SSSGGAITTGYPGPRSPNPKSDIRRKPVSPSQSPTGFGKRGW 467
>At3g54940.3 68416.m06091 cysteine proteinase, putative contains
similarity to cysteine proteinase GI:479060 from
[Glycine max]
Length = 368
Score = 29.5 bits (63), Expect = 8.1
Identities = 24/82 (29%), Positives = 36/82 (43%), Gaps = 2/82 (2%)
Query: 124 QFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALA-ERLSD 182
Q A L+ G AV+M + V C L C++ H V+ G+ S + RLS+
Sbjct: 265 QIAANLVRHGPLAVGLNAVFMQTYIGGVSCPLICSKRNVNHGVLLVGYGSKGFSILRLSN 324
Query: 183 AKAKLLVTADGAWRGERMYVYK 204
++ + G GE Y YK
Sbjct: 325 KPYWIIKNSWGKKWGENGY-YK 345
>At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to
polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis
thaliana] (Genetics 139 (2), 921-939 (1995))
Length = 631
Score = 29.5 bits (63), Expect = 8.1
Identities = 26/86 (30%), Positives = 41/86 (47%), Gaps = 7/86 (8%)
Query: 616 KGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAP-DTIQNAPGLPKTRSGKIMRR 674
KG LY + G+Q VKTLT R++I DTI+N + + + G +
Sbjct: 305 KGSTLYLVTRFRCGMQI---FVKTLT---RKRINLEVESWDTIENVKAMVQDKEGIQPQP 358
Query: 675 ILRKIAVGDHEVGDISTLADPGVVEE 700
L+++ E+ D TLAD + +E
Sbjct: 359 NLQRLIFLGKELKDGCTLADYSIQKE 384
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.321 0.138 0.434
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,182,755
Number of Sequences: 28952
Number of extensions: 821855
Number of successful extensions: 1711
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 52
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1543
Number of HSP's gapped (non-prelim): 129
length of query: 714
length of database: 12,070,560
effective HSP length: 86
effective length of query: 628
effective length of database: 9,580,688
effective search space: 6016672064
effective search space used: 6016672064
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 63 (29.5 bits)
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