Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000721-TA|BGIBMGA000721-PA|IPR011904|Acetate--CoA
ligase, IPR000873|AMP-dependent synthetase and ligase
(714 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 383 e-107
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 80 9e-16
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy... 40 0.001
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 38 0.005
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 38 0.006
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 38 0.006
SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces po... 29 3.0
SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 28 3.9
SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog Taz1|Schizosacc... 27 6.8
SPAC13G6.07c |rps601|rps6-1|40S ribosomal protein S6|Schizosacch... 27 9.0
SPAPB1E7.12 |rps602|rps6-2, rps6|40S ribosomal protein S6|Schizo... 27 9.0
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 383 bits (943), Expect = e-107
Identities = 194/453 (42%), Positives = 275/453 (60%), Gaps = 29/453 (6%)
Query: 10 DHVIKKSYLPSIQKYKEMYERSLTESELFWTEVAQEFQ-WHVAPKPGGFMKYNFDITKGR 68
D + K + S+ +YK MYE S+ + FW +A++ W + ++ + D
Sbjct: 21 DPTVPKPNIASLDEYKRMYEESINDPSTFWGNMARDMMTWD--KQFSTVVQGSIDKADSA 78
Query: 69 VFVKFMEEAELNVCYNLLDRNVKNGFGDKIAFFWEGNSPDDYTRITYRKLLDLVCQFANG 128
F + ++ CYNL+DR+ D +A +E + P+ ITYR+LL V Q A
Sbjct: 79 WFA----DGAISPCYNLVDRHAI-ARPDAVALIYEADEPNQGRYITYRELLASVSQCAGA 133
Query: 129 LLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALAERLSDAKAKLL 188
L + G+ GDRVA+YMPMI E +I MLA R+GA+H+V+FAGFS++++A+R++D++ K++
Sbjct: 134 LQSMGVGMGDRVAIYMPMIPETIIAMLAIVRLGAIHSVIFAGFSAESVADRVNDSECKVI 193
Query: 189 VTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVNHLDRVTPGGSLKSEIKDLY 248
+TAD + RG + K + A+ + T K V+ P S+
Sbjct: 194 ITADESHRGGKRIPLKGVVNKALTECPTIKK-------VLVFQRSAEPTASMV------- 239
Query: 249 NWNEETDIWWHEVLEGESNICPPEWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAA 308
E D+WWH+++ CPP V E PLF+LYTSGSTGKPKGVVH T GYL+ AAA
Sbjct: 240 ---EGRDVWWHDIIPKFPRYCPPAVVNPEHPLFLLYTSGSTGKPKGVVHCTGGYLLGAAA 296
Query: 309 TFRYVFDYHRRDVYWCTADVGWITGHTYVVYAPLANAATSVMFEGTPYFPKVDRYWSIIN 368
T +YVFD H D C DVGWITGHTY+VY PL A +++FE TP +P RYWS++
Sbjct: 297 TCKYVFDLHPTDRMGCAGDVGWITGHTYIVYGPLMLGAATLVFESTPAYPDYSRYWSVVE 356
Query: 369 KYKVTQFYTAPTAIRALMAHYNVEDGNLTMPDLSSLRVLGSVGEPINPAAWLWYYTRIGK 428
++++TQ+Y APTAIR L N D SSLRVLGSVGEPI P +++WYY +G+
Sbjct: 357 RHRLTQWYIAPTAIRLLQRAGN----EFVKHDRSSLRVLGSVGEPIAPESFMWYYEVVGE 412
Query: 429 ERCSIVDTFWQTETGGHVITGLPGATPMKPGAA 461
+RC++ DT+WQTETG H++T L TPMKPG+A
Sbjct: 413 KRCAVADTYWQTETGSHIVTSLGPVTPMKPGSA 445
Score = 198 bits (482), Expect = 3e-51
Identities = 100/206 (48%), Positives = 135/206 (65%), Gaps = 4/206 (1%)
Query: 501 PFFGVEPKILNE-DGVEIEGPG-EGYLVFARPWPGMMRTLLEDQDRFERVYFSKFPGYYC 558
PFFG++ I++ G IEG EG L PWP RT+ DR+ Y +PG+Y
Sbjct: 448 PFFGIDAVIIDPLTGKIIEGNDVEGVLAIRSPWPSAARTVWRGHDRYIDTYLKPYPGFYF 507
Query: 559 TGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVKGE 618
TGDG RD+DGY+WI GR+DD++N+SGH +STAE+E L S V+E+AVV E+ G+
Sbjct: 508 TGDGATRDKDGYIWIRGRVDDVVNISGHRLSTAEIEAALLSHDAVAESAVVGVHDELTGQ 567
Query: 619 ALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRILRK 678
A+ F++LK G + +L K L + VR IG FA+P + + LPKTRSGKIMRRILRK
Sbjct: 568 AVNAFILLKPGYEATVELEKELIMAVRSTIGPFASPRKLIFS-DLPKTRSGKIMRRILRK 626
Query: 679 IAVGD-HEVGDISTLADPGVVEELFN 703
I G+ ++GD+STLADP VVE + +
Sbjct: 627 ILAGEVDQIGDLSTLADPKVVEHIIH 652
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 80.2 bits (189), Expect = 9e-16
Identities = 56/176 (31%), Positives = 85/176 (48%), Gaps = 11/176 (6%)
Query: 503 FGVEPKILNEDGVEIEGPGEGYLVFA--RPWPGMMRTLLEDQDRFERVYFSKFPGYYCTG 560
FGVE KIL++ G E+ EG + G + ++ F + F + TG
Sbjct: 334 FGVELKILDQKGNEMPQGKEGEICVRGINVTKGYLNNPAANKSSFTKDRFFR------TG 387
Query: 561 DGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVKGEAL 620
D + D+DGY++ITGRI +++N G +S AE++ VL P VSEA P E G+ +
Sbjct: 388 DEGKLDKDGYVFITGRIKELVNRGGEKISPAEIDAVLMQHPDVSEAVCFAVPDEKYGQDI 447
Query: 621 YCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRRIL 676
+ G K L + QK+ AF P +PKT +GK+ RR++
Sbjct: 448 QAAINPVAGKTVTP---KQLHDYLEQKVAAFKIPKKFYFTDRIPKTATGKVQRRLV 500
Score = 33.5 bits (73), Expect = 0.10
Identities = 28/110 (25%), Positives = 48/110 (43%), Gaps = 5/110 (4%)
Query: 277 EDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTY 336
+D + VL+TSG+TG+PK VV T L + + RD + + + G
Sbjct: 161 DDVMLVLHTSGTTGRPK-VVPLTHKNLCRSIHNITTSYRLDPRDTSYVVMPLFHVHGLLC 219
Query: 337 VVYAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALM 386
+ + LA+ +V+ P F +W +Y T + PT + L+
Sbjct: 220 GLLSTLASGGCAVV---PPKF-SAHSFWKEFIQYGATWYTAVPTIHQILL 265
>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 4924
Score = 39.9 bits (89), Expect = 0.001
Identities = 22/79 (27%), Positives = 41/79 (51%)
Query: 112 RITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGF 171
R+TY +L + + A+ L + G R G +AVY +EA I MLA + G +
Sbjct: 2751 RLTYSELNERANKLAHNLKSYGFRVGSIIAVYFDKCIEAFISMLAILKAGCCFLALDVSA 2810
Query: 172 SSDALAERLSDAKAKLLVT 190
++ + ++D+ A L+++
Sbjct: 2811 PTERIRYIVTDSTAVLVMS 2829
Score = 34.7 bits (76), Expect = 0.045
Identities = 30/114 (26%), Positives = 46/114 (40%), Gaps = 6/114 (5%)
Query: 281 FVLYTSGSTGKPKGVV--HTTAGYLMYAAATFRYVFDYHRRDVYWCTADVGWITGHTYVV 338
+VLYTSGSTG PKGV H A + + +F R D + A+V + + +
Sbjct: 363 YVLYTSGSTGNPKGVAISHRAATNSIKSHGYLYPMFGLERGDAWLQFANVTFDVS-VFEI 421
Query: 339 YAPLANAATSVMFEGTPYFPKVDRYWSIINKYKVTQFYTAPTAIRALMAHYNVE 392
+ N T V + ++ +I YK+ PT + N E
Sbjct: 422 FGNWNNGLTLVTSKRQNLIGNLE---YLIYDYKIAALELTPTVANVISLDENKE 472
Score = 34.3 bits (75), Expect = 0.059
Identities = 40/138 (28%), Positives = 59/138 (42%), Gaps = 20/138 (14%)
Query: 557 YCTGDGVRRDEDGYLWITGRI--DDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHE 614
Y TGD + R +G L+I GRI D + + G + E+E LA P V A+ E
Sbjct: 593 YRTGD-LGRIINGKLYIFGRIAGDMQIKIRGRRIEIGEIESKLA--PSVDSLAI-----E 644
Query: 615 VKGEALYCFVVLKEGLQFDADLVKTLTLLVRQKIGAFAAPDTIQNAPGLPKTRSGKIMRR 674
G+ L F V D +K L + ++ P PG P+ SGK R+
Sbjct: 645 KIGDNLVAFYV--------GDEMK-LRKHAESHLASWMCPTKYVGLPGFPRLASGKTDRK 695
Query: 675 ILR-KIAVGDHEVGDIST 691
L+ + + D D S+
Sbjct: 696 ALKIQFSASDKHSTDFSS 713
Score = 32.7 bits (71), Expect = 0.18
Identities = 13/14 (92%), Positives = 14/14 (100%)
Query: 281 FVLYTSGSTGKPKG 294
+VLYTSGSTGKPKG
Sbjct: 2874 YVLYTSGSTGKPKG 2887
Score = 29.5 bits (63), Expect = 1.7
Identities = 20/57 (35%), Positives = 27/57 (47%)
Query: 133 GIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAGFSSDALAERLSDAKAKLLV 189
GI+K D VAV++ L+ ML RIGA + S + E L KLL+
Sbjct: 1801 GIKKNDVVAVFIDKSLDYYSLMLGVLRIGAFFFPLEHCSSLNFAKENLLRTNVKLLI 1857
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 37.9 bits (84), Expect = 0.005
Identities = 24/84 (28%), Positives = 41/84 (48%)
Query: 111 TRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILEAVICMLACARIGAVHTVVFAG 170
T TYR++ + A+ L+ GI +GD V VY ++ V+ ++ + GA +V+
Sbjct: 311 TSYTYRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPA 370
Query: 171 FSSDALAERLSDAKAKLLVTADGA 194
+ LS AK + LV + A
Sbjct: 371 YPPARQIIYLSVAKPRALVVLEDA 394
Score = 30.7 bits (66), Expect = 0.73
Identities = 20/77 (25%), Positives = 35/77 (45%), Gaps = 1/77 (1%)
Query: 557 YCTGDGVRRDEDGYLWITGRIDDMLNVSGHLMSTAEVEGVLASDPRVSEAAVVPKPHEVK 616
Y +GD R G + +GR DD + + G + E+ L+ P V E + + + +
Sbjct: 721 YRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRENITLVRRDKDE 780
Query: 617 GEALYCFVVLKEGLQFD 633
L ++V +GL D
Sbjct: 781 EPTLVAYIV-PQGLNKD 796
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 37.5 bits (83), Expect = 0.006
Identities = 18/33 (54%), Positives = 21/33 (63%)
Query: 264 GESNICPPEWVAAEDPLFVLYTSGSTGKPKGVV 296
G+ PP A+D +YTSGSTGKPKGVV
Sbjct: 232 GKEKPQPPHPPKADDICCYMYTSGSTGKPKGVV 264
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 37.5 bits (83), Expect = 0.006
Identities = 52/232 (22%), Positives = 88/232 (37%), Gaps = 24/232 (10%)
Query: 90 VKNGFGDKIAFFWEGNSPDDYTRITYRKLLDLVCQFANGLLAKGIRKGDRVAVYMPMILE 149
V +G ++ W DY +++ + D ++A L G+ KGD+ +Y P
Sbjct: 74 VVDGEKKEVPKSWSYFELSDYNYLSFNDIYDKALRYAGALRKLGLNKGDKFELYAPTSAF 133
Query: 150 AVICMLACARIGAVHTVVFAGFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDD 209
++ AC + T+V A D L E + L + RG MY
Sbjct: 134 WLLTAEAC--LSQSMTIVTA---YDTLGE-----EGLLHSLRESGVRG--MYTEGH---- 177
Query: 210 AIEKARTFFKHRVDMCMVVNHLDRVTPGGSLKSE-IKDLYNWNEETDIWWHEVLEGESNI 268
K V+ + L+ + K E IK + + E S
Sbjct: 178 -------LLKTLVNPLKEIESLEVIIYRNDAKEEDIKTIQEIRPNLKLIKFADFEKMSPP 230
Query: 269 CPPEWVAAEDPLFVLYTSGSTGKPKGVVHTTAGYLMYAAATFRYVFDYHRRD 320
P+ + E+ ++YTSGSTG PKGV+ + + A ++V + +D
Sbjct: 231 VEPDPPSPEEICCIMYTSGSTGLPKGVILSHKNMVAIVTAIVKHVPEVTSKD 282
>SPAC30D11.04c |nup124||nucleoporin Nup124|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1159
Score = 28.7 bits (61), Expect = 3.0
Identities = 21/63 (33%), Positives = 28/63 (44%), Gaps = 6/63 (9%)
Query: 450 LPGATPMKPGAAEKKHPVYYSSMDTFRPKQGWDRTVMIVHLPNKSNRTRGFPFFGVEPKI 509
LP T P E K P ++S + PK+ + T + P+ T GF F G PK
Sbjct: 527 LPSKTQETPSITENK-PSFFSQLS---PKR--EETEKKDNAPSAPASTSGFSFGGFAPKT 580
Query: 510 LNE 512
L E
Sbjct: 581 LEE 583
>SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1316
Score = 28.3 bits (60), Expect = 3.9
Identities = 17/63 (26%), Positives = 32/63 (50%), Gaps = 6/63 (9%)
Query: 170 GFSSDALAERLSDAKAKLLVTADGAWRGERMYVYKRTCDDAIEKARTFFKHRVDMCMVVN 229
GF + LA+ L K + + + D E V R CD I+ +F+H ++C++++
Sbjct: 154 GFGAQLLAQHLE--KLQTVPSTDSLPPFET--VLCRVCDHEIQN--WYFEHHTELCLLIH 207
Query: 230 HLD 232
H +
Sbjct: 208 HAE 210
>SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog
Taz1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 663
Score = 27.5 bits (58), Expect = 6.8
Identities = 13/53 (24%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Query: 549 YFSKFPGYYCTGDGVRRDEDGYLWITGRIDDMLNVSGHL-MSTAEVEGVLASD 600
Y K+ Y+C + + + + TG ID + N + HL + + ++ +L++D
Sbjct: 206 YIRKYDAYWCLFEDLEKPLKSIQFFTGLIDLLDNTNKHLTLRSIVLDALLSAD 258
>SPAC13G6.07c |rps601|rps6-1|40S ribosomal protein
S6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 239
Score = 27.1 bits (57), Expect = 9.0
Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Query: 530 PWPGMMRTLLEDQDRFERVYFSKFPGYYCTGDGVRRDEDGYLW-ITGRID 578
P G + + D DR RV+ K G GD V + GY++ ITG D
Sbjct: 8 PANGTQKLIEIDDDRRLRVFMEKRMGQEVPGDSVGPEFAGYVFKITGGND 57
>SPAPB1E7.12 |rps602|rps6-2, rps6|40S ribosomal protein
S6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 239
Score = 27.1 bits (57), Expect = 9.0
Identities = 18/50 (36%), Positives = 24/50 (48%), Gaps = 1/50 (2%)
Query: 530 PWPGMMRTLLEDQDRFERVYFSKFPGYYCTGDGVRRDEDGYLW-ITGRID 578
P G + + D DR RV+ K G GD V + GY++ ITG D
Sbjct: 8 PANGTQKLIEIDDDRRLRVFMEKRMGQEVPGDSVGPEFAGYVFKITGGND 57
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.321 0.138 0.434
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,442,294
Number of Sequences: 5004
Number of extensions: 152396
Number of successful extensions: 316
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 291
Number of HSP's gapped (non-prelim): 23
length of query: 714
length of database: 2,362,478
effective HSP length: 78
effective length of query: 636
effective length of database: 1,972,166
effective search space: 1254297576
effective search space used: 1254297576
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 57 (27.1 bits)
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