Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000710-TA|BGIBMGA000710-PA|IPR000535|Major sperm
protein, IPR008962|PapD-like
(248 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60600.1 68416.m06781 vesicle-associated membrane protein, pu... 75 5e-14
At2g45140.1 68415.m05618 vesicle-associated membrane protein, pu... 73 2e-13
At4g00170.1 68417.m00018 vesicle-associated membrane family prot... 70 2e-12
At5g47180.2 68418.m05818 vesicle-associated membrane family prot... 66 2e-11
At5g47180.1 68418.m05817 vesicle-associated membrane family prot... 66 2e-11
At1g08820.1 68414.m00982 vesicle-associated membrane family prot... 65 4e-11
At2g23830.1 68415.m02847 vesicle-associated membrane protein, pu... 65 5e-11
At1g51270.1 68414.m05766 vesicle-associated membrane protein, pu... 54 9e-08
At5g54110.1 68418.m06737 vesicle-associated membrane family prot... 42 5e-04
At4g21450.1 68417.m03103 vesicle-associated membrane family prot... 38 0.006
At4g21450.2 68417.m03102 vesicle-associated membrane family prot... 37 0.015
At4g05060.1 68417.m00746 vesicle-associated membrane family prot... 36 0.034
At1g77500.1 68414.m09025 expressed protein contains Pfam domains... 30 1.3
At3g57710.1 68416.m06429 protein kinase family protein contains ... 29 2.9
At5g54070.1 68418.m06731 heat shock transcription factor family ... 29 3.9
At4g15890.1 68417.m02415 expressed protein 29 3.9
At5g11430.1 68418.m01335 transcription elongation factor-related... 28 6.8
At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative / phos... 27 9.0
>At3g60600.1 68416.m06781 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 256
Score = 74.9 bits (176), Expect = 5e-14
Identities = 40/82 (48%), Positives = 53/82 (64%), Gaps = 6/82 (7%)
Query: 3 LTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPVYLDPNENH-KHKFMV 61
LTN T++ V FK+KTT PKKYCVRPN+GV+ P S E+ +T Q P++ K KF++
Sbjct: 46 LTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRSTCEVLVTMQAQKEAPSDMQCKDKFLL 105
Query: 62 QSVIAPEGKTNIDQVWKEISPE 83
Q VIA G T KE++PE
Sbjct: 106 QGVIASPGVTA-----KEVTPE 122
>At2g45140.1 68415.m05618 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 239
Score = 72.9 bits (171), Expect = 2e-13
Identities = 41/99 (41%), Positives = 58/99 (58%), Gaps = 4/99 (4%)
Query: 3 LTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPVYLDPNE-NHKHKFMV 61
L N T++ V FK+KTT PKKYCVRPN+GV+ P S E+ +T Q P + K KF++
Sbjct: 29 LGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPRSSSEVLVTMQAQKEAPADLQCKDKFLL 88
Query: 62 QSVIAPEGKTNIDQVWKEISPE---QLMDYKLKCVFETP 97
Q V+A G T D + S E ++ + KL+ V+ P
Sbjct: 89 QCVVASPGATPKDVTHEMFSKEAGHRVEETKLRVVYVAP 127
>At4g00170.1 68417.m00018 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 239
Score = 69.7 bits (163), Expect = 2e-12
Identities = 37/99 (37%), Positives = 60/99 (60%), Gaps = 5/99 (5%)
Query: 1 MRLTNPTN-DTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPVYLDPNENH-KHK 58
M+LTN T V FK+KTT P+KYCVRPN+GV+ P + +T Q P + K K
Sbjct: 28 MQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDSCNVTVTMQAQKEAPLDMQCKDK 87
Query: 59 FMVQSVIAPEGKTN---IDQVWKEISPEQLMDYKLKCVF 94
F+VQ+V+ +G T+ + +++ + + + D+KL+ V+
Sbjct: 88 FLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVVY 126
>At5g47180.2 68418.m05818 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 66.1 bits (154), Expect = 2e-11
Identities = 43/118 (36%), Positives = 64/118 (54%), Gaps = 7/118 (5%)
Query: 1 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPV-YLDPNENHKHKF 59
+++ N T + V FK+KTT+PKKY VRPN+GV+ P I +T Q P+ K KF
Sbjct: 31 LKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDSCIIRVTLQAQREYPPDMQCKDKF 90
Query: 60 MVQSVIAPEGKTNIDQV----WKEISPEQLMDYKLKCVFETPRGTNLNDSGDNAAQND 113
++QS I P T++D++ + + S + L + KLK + TP T S A D
Sbjct: 91 LLQSTIVPP-HTDVDELPQDTFTKDSGKTLTECKLKVSYITP-STTQRSSESGATNGD 146
>At5g47180.1 68418.m05817 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia], to VAMP-associated protein
B GI:4240464 [Rattus norvegicus] and to
Vesicle-associated membrane protein/synaptobrevin
binding protein (VAP-33) (SP:Q16943)[Aplysia
californica]
Length = 220
Score = 66.1 bits (154), Expect = 2e-11
Identities = 43/118 (36%), Positives = 64/118 (54%), Gaps = 7/118 (5%)
Query: 1 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPV-YLDPNENHKHKF 59
+++ N T + V FK+KTT+PKKY VRPN+GV+ P I +T Q P+ K KF
Sbjct: 31 LKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDSCIIRVTLQAQREYPPDMQCKDKF 90
Query: 60 MVQSVIAPEGKTNIDQV----WKEISPEQLMDYKLKCVFETPRGTNLNDSGDNAAQND 113
++QS I P T++D++ + + S + L + KLK + TP T S A D
Sbjct: 91 LLQSTIVPP-HTDVDELPQDTFTKDSGKTLTECKLKVSYITP-STTQRSSESGATNGD 146
>At1g08820.1 68414.m00982 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 386
Score = 65.3 bits (152), Expect = 4e-11
Identities = 34/75 (45%), Positives = 45/75 (60%), Gaps = 1/75 (1%)
Query: 1 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPVYLDPNEN-HKHKF 59
++LTN T+ V FK+KTT+PKKYCVRPN GV+AP S E + Q P + K KF
Sbjct: 27 VQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAPKSTCEFTVIMQAFKEPPPDMVCKDKF 86
Query: 60 MVQSVIAPEGKTNID 74
++QS T+ D
Sbjct: 87 LIQSTAVSAETTDED 101
>At2g23830.1 68415.m02847 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 149
Score = 64.9 bits (151), Expect = 5e-11
Identities = 37/107 (34%), Positives = 59/107 (55%), Gaps = 4/107 (3%)
Query: 3 LTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPVYLDPNENHK-HKFMV 61
LTN T++ V FK+KTT YCVRPN G++ P S ++ +T Q P++ KFM+
Sbjct: 30 LTNKTDNNVAFKVKTTNRNNYCVRPNYGLILPKSTCKVLVTMQAQKEVPSDMQSFEKFMI 89
Query: 62 QSVIAPEGKTNID---QVWKEISPEQLMDYKLKCVFETPRGTNLNDS 105
QSV+A G T + +++ + S + + KL+ + TN+ S
Sbjct: 90 QSVLASPGVTAKEVTREMFSKESGHVVEETKLRVTYVCSTTTNITSS 136
>At1g51270.1 68414.m05766 vesicle-associated membrane protein,
putative / VAMP, putative similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 289
Score = 54.0 bits (124), Expect = 9e-08
Identities = 34/101 (33%), Positives = 53/101 (52%), Gaps = 4/101 (3%)
Query: 1 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPVYLDPNENH-KHKF 59
+ LTN T + V FK KTT KKY VRPN GV+ P S E+ + Q + P + + K
Sbjct: 150 LNLTNKTENYVAFKAKTTNAKKYYVRPNVGVVLPRSSCEVLVIMQALKEAPADMQCRDKL 209
Query: 60 MVQ-SVIAPE--GKTNIDQVWKEISPEQLMDYKLKCVFETP 97
+ Q V+ PE K +++ + + + +LK ++ TP
Sbjct: 210 LFQCKVVEPETTAKDVTSEMFSKEAGHPAEETRLKVMYVTP 250
Score = 31.1 bits (67), Expect = 0.73
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Query: 23 YCVRPNSGVLAPNSKQEIAITPQPVYLDPNENH-KHKFMVQSVIAPEG 69
YCV+P+ GV+ P S E+ + Q + P + K K + Q + G
Sbjct: 2 YCVKPSVGVVLPRSSCEVLVVMQALKEAPADRQCKDKLLFQCKVVEPG 49
>At5g54110.1 68418.m06737 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 266
Score = 41.5 bits (93), Expect = 5e-04
Identities = 27/100 (27%), Positives = 46/100 (46%), Gaps = 6/100 (6%)
Query: 1 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAI-----TPQPVYLDP-NEN 54
++L N + FK +TTAPK +RP GVLAP + P+ P N+
Sbjct: 99 IKLKNTSKSHTAFKFQTTAPKSCYMRPPGGVLAPGESVFATVFKFVEHPENNEKQPLNQK 158
Query: 55 HKHKFMVQSVIAPEGKTNIDQVWKEISPEQLMDYKLKCVF 94
K KF + S+ G + +++ E + ++ L+ +F
Sbjct: 159 SKVKFKIMSLKVKPGVEYVPELFDEQKDQVAVEQVLRVIF 198
>At4g21450.1 68417.m03103 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 295
Score = 37.9 bits (84), Expect = 0.006
Identities = 27/109 (24%), Positives = 50/109 (45%), Gaps = 11/109 (10%)
Query: 1 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPVYLDPNENHKH--- 57
+++ N + V FK +TTAPK +RP +LAP E I +++P EN++
Sbjct: 130 IKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPG---ETIIATVFKFVEPPENNEKPMD 186
Query: 58 -----KFMVQSVIAPEGKTNIDQVWKEISPEQLMDYKLKCVFETPRGTN 101
KF + S+ + +++ E + + L+ +F P +N
Sbjct: 187 QRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERSN 235
>At4g21450.2 68417.m03102 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 212
Score = 36.7 bits (81), Expect = 0.015
Identities = 19/56 (33%), Positives = 31/56 (55%), Gaps = 3/56 (5%)
Query: 1 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPVYLDPNENHK 56
+++ N + V FK +TTAPK +RP +LAP E I +++P EN++
Sbjct: 130 IKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPG---ETIIATVFKFVEPPENNE 182
>At4g05060.1 68417.m00746 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 287
Score = 35.5 bits (78), Expect = 0.034
Identities = 19/56 (33%), Positives = 32/56 (57%), Gaps = 3/56 (5%)
Query: 1 MRLTNPTNDTVLFKIKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPVYLDPNENHK 56
+++ N + V FK +TT PK +RP +LAP +EI T +++P EN++
Sbjct: 122 IKIKNTSKSHVAFKFQTTVPKSCFMRPAGAILAPG--EEIIATVFK-FVEPPENNE 174
>At1g77500.1 68414.m09025 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 879
Score = 30.3 bits (65), Expect = 1.3
Identities = 20/85 (23%), Positives = 33/85 (38%), Gaps = 2/85 (2%)
Query: 138 QHEDKNKDDDHPVASINLKTTNTKSENVESDLQKATLEVIHLREEESKLRHENXXXXXXX 197
+H D++K + P +IN + + ES L T+ + ES+ + N
Sbjct: 331 EHRDEHKHNVFPERNINKREVPMPEQVTESSLDSETISSFSGSDVESEFHYVNGGEGKSS 390
Query: 198 XXXXXSAGGDGRVSR--PHSYGPEK 220
S G + SR YG +K
Sbjct: 391 SISSISHGAGTKSSREVEEQYGRKK 415
>At3g57710.1 68416.m06429 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 351
Score = 29.1 bits (62), Expect = 2.9
Identities = 13/36 (36%), Positives = 19/36 (52%)
Query: 42 ITPQPVYLDPNENHKHKFMVQSVIAPEGKTNIDQVW 77
+ P V+LD N K + S+ PEGK+ I+ W
Sbjct: 192 VKPMHVFLDKNWTAKLSDLSFSISLPEGKSRIEAEW 227
>At5g54070.1 68418.m06731 heat shock transcription factor family
protein contains Pfam profile: PF00447 HSF-type
DNA-binding domain
Length = 331
Score = 28.7 bits (61), Expect = 3.9
Identities = 15/42 (35%), Positives = 23/42 (54%)
Query: 148 HPVASINLKTTNTKSENVESDLQKATLEVIHLREEESKLRHE 189
H + +I ++ NTK N E+ EV L+EE+S +R E
Sbjct: 153 HLLKNIKRRSKNTKCCNKEASTTTTETEVESLKEEQSPMRLE 194
>At4g15890.1 68417.m02415 expressed protein
Length = 1314
Score = 28.7 bits (61), Expect = 3.9
Identities = 18/87 (20%), Positives = 36/87 (41%), Gaps = 4/87 (4%)
Query: 15 IKTTAPKKYCVRPNSGVLAPNSKQEIAITPQPV---YLDPNENHKHKFMVQSVIAPEGKT 71
+KT KK + +L +++++ V Y++ +H H F+ + +
Sbjct: 734 LKTLCKKKASTSEEADMLVKKWVEQVSLKASKVTEKYIEGVSSHNHSFVTPATLGSRRSK 793
Query: 72 NIDQVWKEISPEQLMDYKL-KCVFETP 97
+D V K++S Y + CV P
Sbjct: 794 RLDTVSKKLSKAVTAVYTIGSCVIIYP 820
>At5g11430.1 68418.m01335 transcription elongation factor-related
contains weak similarity to transcription elongation
factors
Length = 871
Score = 27.9 bits (59), Expect = 6.8
Identities = 11/40 (27%), Positives = 20/40 (50%)
Query: 29 SGVLAPNSKQEIAITPQPVYLDPNENHKHKFMVQSVIAPE 68
+ VL N KQ + +T +P+ + NH H ++ A +
Sbjct: 708 TSVLPVNKKQRVHVTEKPLVVASMRNHHHGYVKHDTAADD 747
>At3g24840.1 68416.m03116 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; similar to SEC14
CYTOSOLIC FACTOR (PHOSPHATIDYLINOSITOL/
PHOSPHATIDYLCHOLINE TRANSFER PROTEIN) GB:P46250 from
[Candida albicans] (Yeast (1996) 12(11), 1097-1105);
contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam
PF03765 : CRAL/TRIO, N-terminus
Length = 579
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/50 (28%), Positives = 25/50 (50%)
Query: 136 LIQHEDKNKDDDHPVASINLKTTNTKSENVESDLQKATLEVIHLREEESK 185
L+ DK K D+ S + K T T+S +++ KA+ ++ H + K
Sbjct: 9 LVPETDKGKSKDYIEVSEDEKITRTRSRSLKKKAIKASNKLTHSLRKRGK 58
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.312 0.128 0.373
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,440,930
Number of Sequences: 28952
Number of extensions: 207504
Number of successful extensions: 391
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 370
Number of HSP's gapped (non-prelim): 19
length of query: 248
length of database: 12,070,560
effective HSP length: 79
effective length of query: 169
effective length of database: 9,783,352
effective search space: 1653386488
effective search space used: 1653386488
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 58 (27.5 bits)
- SilkBase 1999-2023 -