BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000709-TA|BGIBMGA000709-PA|IPR002048|Calcium-binding EF-hand (155 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schi... 191 4e-50 SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyce... 88 6e-19 SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 41 7e-05 SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 38 5e-04 SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 38 0.001 SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces ... 29 0.42 SPAC1687.14c |||EF hand family protein, unknown role|Schizosacch... 28 0.73 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 27 0.97 SPBC3B9.09 |vps36||RBZ zinc finger protein Vps36|Schizosaccharom... 27 1.3 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 27 1.7 SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccha... 26 2.2 SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomy... 25 3.9 SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 25 3.9 SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 5.2 SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosacch... 25 5.2 SPBC2A9.05c |||DUF846 family protein|Schizosaccharomyces pombe|c... 25 5.2 SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 6.8 SPAC1783.03 |fta2|sma2|Sim4 and Mal2 associated |Schizosaccharom... 25 6.8 SPAC25A8.03c ||SPAC3C7.15c|DUF185 protein|Schizosaccharomyces po... 25 6.8 SPBC2G5.07c |rpc25||DNA-directed RNA polymerase III complex subu... 24 9.0 SPAC1805.01c |ppk6|SPAPJ736.02c|serine/threonine protein kinase ... 24 9.0 SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 24 9.0 SPAC29B12.04 |snz1||pyridoxine biosynthesis protein|Schizosaccha... 24 9.0 >SPAC18B11.04 |ncs1||related to neuronal calcium sensor Ncs1|Schizosaccharomyces pombe|chr 1|||Manual Length = 190 Score = 191 bits (466), Expect = 4e-50 Identities = 94/138 (68%), Positives = 111/138 (80%), Gaps = 1/138 (0%) Query: 16 FIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSRGNLDEKLHWAFRL 75 F KIYKQFFP GDPS FA VF VFD + +G I+F+EFI ALSVTSRG L++KL WAF+L Sbjct: 48 FQKIYKQFFPFGDPSAFAEYVFNVFDADKNGYIDFKEFICALSVTSRGELNDKLIWAFQL 107 Query: 76 YDVDNDGYITRDEMYNIVDAIYQMVGQTPQ-PEDENTPQKRVDKIFDQMDKNHDDRLTLE 134 YD+DN+G I+ DEM IVDAIY+MVG + PEDE+TP+KRV+KIF+ MDKN D +LTLE Sbjct: 108 YDLDNNGLISYDEMLRIVDAIYKMVGSMVKLPEDEDTPEKRVNKIFNMMDKNKDGQLTLE 167 Query: 135 EFREGSKADPRIVQALSL 152 EF EGSK DP IV ALSL Sbjct: 168 EFCEGSKRDPTIVSALSL 185 >SPCC830.06 |||calcineurin regulatory subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 174 Score = 87.8 bits (208), Expect = 6e-19 Identities = 48/115 (41%), Positives = 74/115 (64%), Gaps = 7/115 (6%) Query: 25 PQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTS-RGNLDEKLHWAFRLYDVDNDGY 83 P + AS +F V DE+ G ++F+EFI +LSV S GN +EKL +AF++YD+D DGY Sbjct: 50 PSVASNPLASRLFSVVDEDGGGDVDFQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGY 109 Query: 84 ITRDEMYNIVDAIYQMVGQTPQPEDENTPQKRVDKIFDQMDKNHDDRLTLEEFRE 138 I+ E+Y + + MVG T ED+ Q+ VDK ++DK+ D +++ EEF++ Sbjct: 110 ISNGELYLV---LKMMVG-TNLREDQ--LQQIVDKTIMEVDKDRDGKISFEEFKD 158 Score = 31.1 bits (67), Expect = 0.079 Identities = 24/86 (27%), Positives = 35/86 (40%), Gaps = 5/86 (5%) Query: 13 FQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSRGNLDEK---- 68 FQ FI F G+ + F+++D + DG I E L + NL E Sbjct: 75 FQEFINSLSVFSVHGNKEEKLKFAFKIYDIDRDGYISNGELYLVLKMMVGTNLREDQLQQ 134 Query: 69 -LHWAFRLYDVDNDGYITRDEMYNIV 93 + D D DG I+ +E +IV Sbjct: 135 IVDKTIMEVDKDRDGKISFEEFKDIV 160 Score = 24.6 bits (51), Expect = 6.8 Identities = 19/55 (34%), Positives = 24/55 (43%), Gaps = 4/55 (7%) Query: 37 FRVFDENNDGSIEFEEFIRALSVTSRGNLDEKLHWAFRLYDVDNDGYITRDEMYN 91 F D N GSI+ EF+ SV S L +L F + D D G + E N Sbjct: 30 FIKIDANQSGSIDRNEFLSIPSVAS-NPLASRL---FSVVDEDGGGDVDFQEFIN 80 >SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 41.1 bits (92), Expect = 7e-05 Identities = 17/63 (26%), Positives = 42/63 (66%), Gaps = 1/63 (1%) Query: 41 DENNDGSIEFEEFIRALSVTSRG-NLDEKLHWAFRLYDVDNDGYITRDEMYNIVDAIYQM 99 D + +G+I+F EF+ ++ + + +E++ AF+++D D +GYIT +E+ +++ ++ + Sbjct: 58 DADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGER 117 Query: 100 VGQ 102 + Q Sbjct: 118 LSQ 120 Score = 34.7 bits (76), Expect = 0.006 Identities = 20/83 (24%), Positives = 35/83 (42%) Query: 13 FQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSRGNLDEKLHWA 72 F F+ + + D + F+VFD++ +G I EE L+ E++ Sbjct: 67 FTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADM 126 Query: 73 FRLYDVDNDGYITRDEMYNIVDA 95 R D D DG I +E ++ + Sbjct: 127 IREADTDGDGVINYEEFSRVISS 149 Score = 27.9 bits (59), Expect = 0.73 Identities = 17/49 (34%), Positives = 28/49 (57%), Gaps = 7/49 (14%) Query: 59 VTSRGNLDEKL---HWAFRLYDVDNDGYITRDEMYNIVDAIYQMVGQTP 104 +T+R DE++ AF L+D D DG IT +E+ + + +GQ+P Sbjct: 1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNEL----GVVMRSLGQSP 45 >SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 38.3 bits (85), Expect = 5e-04 Identities = 19/86 (22%), Positives = 36/86 (41%) Query: 8 KPFCVFQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSRGNLDE 67 K + + F+++ + + DP + F +FD++ G I R + D+ Sbjct: 87 KGYLQMEDFVRVMTEKIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQ 146 Query: 68 KLHWAFRLYDVDNDGYITRDEMYNIV 93 +L +D+D DG I E I+ Sbjct: 147 ELEAMIEEFDLDQDGEINEQEFIAIM 172 Score = 36.7 bits (81), Expect = 0.002 Identities = 23/103 (22%), Positives = 49/103 (47%), Gaps = 14/103 (13%) Query: 36 VFRVFDENNDGSIEFEEFIRALS--VTSRGNLDEKLHWAFRLYDVDNDGYITRDEMYNIV 93 + R FD+ G ++ E+F+R ++ + R L+E + AF L+D D G I+ + + Sbjct: 78 ILRDFDKTGKGYLQMEDFVRVMTEKIVERDPLEE-IKRAFELFDDDETGKISLRNLRRVA 136 Query: 94 DAIYQMVGQTPQPEDENTPQKRVDKIFDQMDKNHDDRLTLEEF 136 + +EN + ++ + ++ D + D + +EF Sbjct: 137 KEL-----------NENIDDQELEAMIEEFDLDQDGEINEQEF 168 >SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 834 Score = 37.5 bits (83), Expect = 0.001 Identities = 15/67 (22%), Positives = 36/67 (53%) Query: 30 SKFASLVFRVFDENNDGSIEFEEFIRALSVTSRGNLDEKLHWAFRLYDVDNDGYITRDEM 89 S F +F FD++ GS+ ++ + ++ ++ + + F LYD + DG++ + ++ Sbjct: 578 SSFLRHLFLRFDKSMTGSLSLQDLVSGIAELKFRDVMRNISFIFELYDFNGDGFMDKPDV 637 Query: 90 YNIVDAI 96 + +AI Sbjct: 638 LKVSEAI 644 >SPAPB1E7.07 |glt1||glutamate synthase Glt1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2111 Score = 28.7 bits (61), Expect = 0.42 Identities = 19/80 (23%), Positives = 37/80 (46%), Gaps = 3/80 (3%) Query: 77 DVDNDGYITRDEMYNIVDAIYQMVGQTPQPEDENTPQKRVDKIFDQMDKNHDDRLTL--E 134 +V +G ++ DE++ + DA+ G T P D P + I M+ H + +L Sbjct: 1827 EVGKNGDVSLDELHKVYDAVVLASGST-VPRDLPIPNRDSKGIHFAMEFLHKNTKSLLDS 1885 Query: 135 EFREGSKADPRIVQALSLGG 154 E ++G+ + + +GG Sbjct: 1886 ELKDGNYISAKGKDVIVIGG 1905 >SPAC1687.14c |||EF hand family protein, unknown role|Schizosaccharomyces pombe|chr 1|||Manual Length = 76 Score = 27.9 bits (59), Expect = 0.73 Identities = 12/30 (40%), Positives = 19/30 (63%) Query: 60 TSRGNLDEKLHWAFRLYDVDNDGYITRDEM 89 T R +DE+ AF L+DV + GYI +++ Sbjct: 5 TKRLEMDEEAEEAFDLFDVTHKGYIDFEDL 34 Score = 24.2 bits (50), Expect = 9.0 Identities = 15/56 (26%), Positives = 26/56 (46%), Gaps = 2/56 (3%) Query: 33 ASLVFRVFDENNDGSIEFEEFIRALSVTSRGNLDEKLHWAFRLYDVDNDGYITRDE 88 A F +FD + G I+FE+ R+ + E+L L +G ++R+E Sbjct: 14 AEEAFDLFDVTHKGYIDFEDLRRSCAQLGENLTKEQLQLMLDL--AGTNGKVSREE 67 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 27.5 bits (58), Expect = 0.97 Identities = 20/83 (24%), Positives = 40/83 (48%), Gaps = 4/83 (4%) Query: 12 VFQGFIKIYKQFFPQGDPSKFASLVFRVFDENNDGSIEFEEFIRALSVTSRGNLDEKLHW 71 +F Y FP D S L +VF+ ++ +I+F+E R L++ + +++ +L Sbjct: 400 IFSVLFSEYFMIFPHSDQS----LQNKVFELHSTLAIKFDELFRLLNLENEESVEWRLIK 455 Query: 72 AFRLYDVDNDGYITRDEMYNIVD 94 Y ++ D YI + + +D Sbjct: 456 KVFEYRLNLDLYILKQFYCHYLD 478 >SPBC3B9.09 |vps36||RBZ zinc finger protein Vps36|Schizosaccharomyces pombe|chr 2|||Manual Length = 467 Score = 27.1 bits (57), Expect = 1.3 Identities = 12/25 (48%), Positives = 15/25 (60%), Gaps = 5/25 (20%) Query: 68 KLHWAF-----RLYDVDNDGYITRD 87 +LHW+ RLY + DGYI RD Sbjct: 413 RLHWSIGVTLERLYQAEMDGYIVRD 437 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 26.6 bits (56), Expect = 1.7 Identities = 14/46 (30%), Positives = 21/46 (45%) Query: 37 FRVFDENNDGSIEFEEFIRALSVTSRGNLDEKLHWAFRLYDVDNDG 82 FRVFD++N G IE +F + D ++ + D N G Sbjct: 84 FRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSG 129 Score = 25.4 bits (53), Expect = 3.9 Identities = 10/19 (52%), Positives = 14/19 (73%) Query: 66 DEKLHWAFRLYDVDNDGYI 84 +E+ AFR++D DN GYI Sbjct: 77 EEEYIKAFRVFDKDNSGYI 95 Score = 24.6 bits (51), Expect = 6.8 Identities = 9/30 (30%), Positives = 19/30 (63%) Query: 67 EKLHWAFRLYDVDNDGYITRDEMYNIVDAI 96 +++ AF LYD+D DG I + +++ ++ Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSL 38 >SPCC1259.11c |gyp2||GTPase activating protein Gyp2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 720 Score = 26.2 bits (55), Expect = 2.2 Identities = 21/102 (20%), Positives = 44/102 (43%), Gaps = 9/102 (8%) Query: 42 ENNDGSIEFEEFIRALSVTSRGNLDEKLH---WAFRLYDVDNDGYITRDEMYNIVDAIYQ 98 ++ + S+ F+ I L R D LH F+LYD+ DG + +E+ + +++ Sbjct: 592 DSTETSLTFKRIIHGLE---RLKADIALHSEILCFQLYDLKRDGTLRTEEVVELSESLIL 648 Query: 99 MVGQTPQPEDE---NTPQKRVDKIFDQMDKNHDDRLTLEEFR 137 + +DE + + F ++T+E+F+ Sbjct: 649 LCCYEGDEKDEERLTVISEFLKSCFSGCQDRRSFQITMEDFQ 690 >SPAC15A10.11 |ubr11||N-end-recognizing protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 2052 Score = 25.4 bits (53), Expect = 3.9 Identities = 11/36 (30%), Positives = 19/36 (52%) Query: 83 YITRDEMYNIVDAIYQMVGQTPQPEDENTPQKRVDK 118 Y +DE Y++VD Y + + E + +KR+ K Sbjct: 1070 YSLKDEYYDLVDPYYFHYNKNEREESDTILKKRLAK 1105 >SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 661 Score = 25.4 bits (53), Expect = 3.9 Identities = 16/51 (31%), Positives = 25/51 (49%), Gaps = 1/51 (1%) Query: 96 IYQMVGQTPQPEDENTPQKRVDKIFDQ-MDKNHDDRLTLEEFREGSKADPR 145 I +MV Q+P + ++ V + + KNH TL R+GS+ PR Sbjct: 468 IDRMVNQSPDTRSVSKSKRLVRETSSGCLFKNHSKSNTLSPRRKGSRHGPR 518 >SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a Pol2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 2199 Score = 25.0 bits (52), Expect = 5.2 Identities = 16/56 (28%), Positives = 29/56 (51%), Gaps = 2/56 (3%) Query: 81 DGYITRDEMY--NIVDAIYQMVGQTPQPEDENTPQKRVDKIFDQMDKNHDDRLTLE 134 DG++ E Y V+++ V ++ P + N K +++ Q+DK D LT+E Sbjct: 530 DGHLLASETYVGGHVESLESGVFRSDLPTNFNMDPKVYEELILQLDKALDFSLTVE 585 >SPBC16D10.05 |mok13||alpha-1,3-glucan synthase Mok13|Schizosaccharomyces pombe|chr 2|||Manual Length = 2358 Score = 25.0 bits (52), Expect = 5.2 Identities = 15/78 (19%), Positives = 31/78 (39%) Query: 42 ENNDGSIEFEEFIRALSVTSRGNLDEKLHWAFRLYDVDNDGYITRDEMYNIVDAIYQMVG 101 E ND IE+ EF L ++ + F V +D + D + + V + + Sbjct: 1695 ETNDQDIEYNEFYSQLDTSTSDIFQDTSVDGFPDLQVSSDINVRNDRLSSFVMSSEDLRS 1754 Query: 102 QTPQPEDENTPQKRVDKI 119 PE+ ++ + + + Sbjct: 1755 SDGHPENSDSVLETISSV 1772 >SPBC2A9.05c |||DUF846 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 219 Score = 25.0 bits (52), Expect = 5.2 Identities = 10/26 (38%), Positives = 16/26 (61%) Query: 61 SRGNLDEKLHWAFRLYDVDNDGYITR 86 SR + D K WA L D ++ G+++R Sbjct: 182 SRCDKDAKRRWATELADSNSSGFVSR 207 >SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 630 Score = 24.6 bits (51), Expect = 6.8 Identities = 10/26 (38%), Positives = 18/26 (69%) Query: 116 VDKIFDQMDKNHDDRLTLEEFREGSK 141 + +IF +DKN+DD L+++E S+ Sbjct: 189 LSRIFFLIDKNNDDLLSVDELNSLSE 214 >SPAC1783.03 |fta2|sma2|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 1|||Manual Length = 351 Score = 24.6 bits (51), Expect = 6.8 Identities = 10/23 (43%), Positives = 16/23 (69%) Query: 118 KIFDQMDKNHDDRLTLEEFREGS 140 K+ +Q+DK + ++L FREGS Sbjct: 316 KLLNQLDKRFSEFVSLFGFREGS 338 >SPAC25A8.03c ||SPAC3C7.15c|DUF185 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 24.6 bits (51), Expect = 6.8 Identities = 13/44 (29%), Positives = 20/44 (45%) Query: 55 RALSVTSRGNLDEKLHWAFRLYDVDNDGYITRDEMYNIVDAIYQ 98 R +S + N LH R Y DN + RD ++ D I++ Sbjct: 24 RVISALAFTNTGLVLHGPRRWYTTDNGFLLHRDSKVSLADYIHE 67 >SPBC2G5.07c |rpc25||DNA-directed RNA polymerase III complex subunit Rpc25|Schizosaccharomyces pombe|chr 2|||Manual Length = 203 Score = 24.2 bits (50), Expect = 9.0 Identities = 11/29 (37%), Positives = 18/29 (62%), Gaps = 1/29 (3%) Query: 11 CVFQGFIKIYKQFFPQGDPSKFASLVFRV 39 CV+ F+KI + GD S + ++VFR+ Sbjct: 47 CVYD-FLKIGEGIIKYGDGSSYMNVVFRL 74 >SPAC1805.01c |ppk6|SPAPJ736.02c|serine/threonine protein kinase Ppk6|Schizosaccharomyces pombe|chr 1|||Manual Length = 775 Score = 24.2 bits (50), Expect = 9.0 Identities = 10/20 (50%), Positives = 14/20 (70%) Query: 116 VDKIFDQMDKNHDDRLTLEE 135 VD I +D+N DR+T+EE Sbjct: 732 VDLICRMLDRNVHDRITIEE 751 >SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces pombe|chr 3|||Manual Length = 587 Score = 24.2 bits (50), Expect = 9.0 Identities = 10/34 (29%), Positives = 17/34 (50%) Query: 87 DEMYNIVDAIYQMVGQTPQPEDENTPQKRVDKIF 120 DE + I++ +MV P D P K +K++ Sbjct: 158 DERHRIIERAMKMVPGFRAPSDYRRPAKTQEKVY 191 >SPAC29B12.04 |snz1||pyridoxine biosynthesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 296 Score = 24.2 bits (50), Expect = 9.0 Identities = 20/54 (37%), Positives = 25/54 (46%), Gaps = 3/54 (5%) Query: 24 FPQGDPSKFASLVFRVFDENNDGSI--EFEEFIRALSV-TSRGNLDEKLHWAFR 74 F GDP+K A + R ND I E E + A V S +L+EK A R Sbjct: 241 FLSGDPAKRARAIVRAVTHYNDPKILAEVSENLGAAMVGRSVSSLEEKEKLATR 294 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.321 0.140 0.420 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 794,677 Number of Sequences: 5004 Number of extensions: 35278 Number of successful extensions: 112 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 17 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 76 Number of HSP's gapped (non-prelim): 35 length of query: 155 length of database: 2,362,478 effective HSP length: 67 effective length of query: 88 effective length of database: 2,027,210 effective search space: 178394480 effective search space used: 178394480 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 50 (24.2 bits)
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