Silkworm BLAST Result against nematostella

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000705-TA|BGIBMGA000705-PA|IPR003929|Calcium-activated
BK potassium channel, alpha subunit
         (1061 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_50107| Best HMM Match : SpoVT_AbrB (HMM E-Value=6.1)               121   4e-27
SB_50109| Best HMM Match : Ion_trans_2 (HMM E-Value=1.4e-10)           62   3e-09
SB_41405| Best HMM Match : RVT_1 (HMM E-Value=0)                       32   2.8  
SB_31410| Best HMM Match : PDZ (HMM E-Value=1.1e-15)                   31   6.5  

>SB_50107| Best HMM Match : SpoVT_AbrB (HMM E-Value=6.1)
          Length = 677

 Score =  121 bits (291), Expect = 4e-27
 Identities = 88/260 (33%), Positives = 135/260 (51%), Gaps = 24/260 (9%)

Query: 667 ITELSQSSNMRFMQFRAHDKYALHLS----------KMEKREKERGSHISYMFRLPFAAG 716
           I EL+ +SNMRFM+F+A   +    S          KM++R K    H++YMFR PF+AG
Sbjct: 400 IVELTYASNMRFMKFQAAPHHVTGESMASLRESTKRKMKERNKNSKDHLNYMFREPFSAG 459

Query: 717 SVFSASMLDTLLYQAFVKDYMITFVRLLLGIDQAPGSGFLASIKITKEDMWIRTYGRLYQ 776
            VFS SM+DTLLYQ FVKDYMIT + LLLG +Q+PGSG+L S         +R  G+   
Sbjct: 460 YVFSMSMIDTLLYQTFVKDYMITLISLLLGCEQSPGSGYLCS-------NTMRARGKRTG 512

Query: 777 KLCSTSCEIPIGIYRTQDTSMADASHHQEELDGGDFPACGRQEATSCLGGCYGERTPVYS 836
            LC    E     Y  ++  +     ++  L     P+   ++  S           VY 
Sbjct: 513 YLCKGRKE--NNTY--EENVVVLGVWYRGNLVSVQGPSQLTKDGHSNCFSLLVRSHRVYE 568

Query: 837 TSLADEARDNHIQQIERAEIANLVRSRMESLNLTGVDYDDVSEKRNSLSYVIINPSCDLN 896
                E       +IE+ +I +++ +RM+ L   G++     +K+   S+V++NP+ + +
Sbjct: 569 APGDCEDACPRGNKIEKRDINDIIGNRMKML---GLNEGPELKKKVGTSFVVVNPAPEFD 625

Query: 897 LQEGDIMNLVIDADTTGKFS 916
           LQEGD++ L+   + TG  S
Sbjct: 626 LQEGDVIYLIRPTNMTGSTS 645



 Score = 86.2 bits (204), Expect = 1e-16
 Identities = 41/82 (50%), Positives = 55/82 (67%), Gaps = 4/82 (4%)

Query: 253 NEIYHIVLGDSRFFGEYEGKSFTYASFHSHRKYGVALVGVRPAELPEFYEDTILLNPGPR 312
           NEIY I LGDS  F  Y  KSFT+A+FH+H+KYGV L+GV+           ++LNPGP 
Sbjct: 11  NEIYDIRLGDSMIFRPYAYKSFTHAAFHAHKKYGVTLIGVK----RHGKGGRLMLNPGPS 66

Query: 313 HIMKSTDTCYYMSITKEENSAF 334
           H M   D C+Y++I+ EE++AF
Sbjct: 67  HNMAYDDRCFYIAISSEEDTAF 88



 Score = 81.8 bits (193), Expect = 3e-15
 Identities = 43/95 (45%), Positives = 61/95 (64%), Gaps = 6/95 (6%)

Query: 550 VKGFPPVSPFIGVSPTLCFLLKEKKPLCCLQLAQV-----CEHCAYRNA-KEYQWQNKTI 603
           + G  PVSP+IG +P  C L +  KP CCL+L +V     CEH    ++ + ++ Q+  I
Sbjct: 247 IVGVLPVSPYIGRTPMRCHLYQTPKPFCCLRLNEVKALIDCEHREMSDSVRMHRQQHGGI 306

Query: 604 ILAADYASNGIYNFIIPLRAHFRSKTTLNPIILLL 638
           I+++  A  G+YNFI+PLRA+ R K TL PIILLL
Sbjct: 307 IVSSPVAEAGLYNFILPLRAYHRPKITLKPIILLL 341


>SB_50109| Best HMM Match : Ion_trans_2 (HMM E-Value=1.4e-10)
          Length = 315

 Score = 61.7 bits (143), Expect = 3e-09
 Identities = 27/49 (55%), Positives = 37/49 (75%)

Query: 41  EQLAFTWMERQKLGGSYSSHRAQSEKHVVVCSTTLHADTIMDFLNEFYA 89
           E+LAF    RQK GG+Y+   A +++HVV+C+TTL   T++DFLNEFYA
Sbjct: 211 ERLAFLLFSRQKEGGAYNHLLAGTDRHVVLCATTLRTGTLIDFLNEFYA 259


>SB_41405| Best HMM Match : RVT_1 (HMM E-Value=0)
          Length = 2639

 Score = 31.9 bits (69), Expect = 2.8
 Identities = 15/38 (39%), Positives = 20/38 (52%)

Query: 1015 ITPPEETPRMALEPFQDIAAALPSTSGGSTMPVNVTPD 1052
            +T  E T    +EP  D+ A+LP+ S   T P   TPD
Sbjct: 567  VTIREHTNLETIEPVTDVVASLPTHSTSYTNPPTTTPD 604


>SB_31410| Best HMM Match : PDZ (HMM E-Value=1.1e-15)
          Length = 556

 Score = 30.7 bits (66), Expect = 6.5
 Identities = 21/75 (28%), Positives = 33/75 (44%), Gaps = 1/75 (1%)

Query: 417 FASSKLADRSTSESRTCLKESPGIESVVDSHLLLPKPETSNFLSPDTLSHRGGNSRRPSI 476
           + + K  ++  S  R+CL     ++ + D   L+PK   S F S     H+  +  +P  
Sbjct: 39  YQTYKSVEKLVSALRSCLDTPRKLDLLADIRNLIPKAHLSKFDSL-VPYHKMAHPIKPPN 97

Query: 477 LPVPDMVTSSLTLTN 491
              PD  T SL L N
Sbjct: 98  QIAPDRKTQSLNLRN 112


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.320    0.134    0.399 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 34,046,922
Number of Sequences: 59808
Number of extensions: 1386210
Number of successful extensions: 2951
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2942
Number of HSP's gapped (non-prelim): 7
length of query: 1061
length of database: 16,821,457
effective HSP length: 90
effective length of query: 971
effective length of database: 11,438,737
effective search space: 11107013627
effective search space used: 11107013627
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 65 (30.3 bits)