GeneModel BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000704-TA|BGIBMGA000704-PA|IPR005123|2OG-Fe(II)
oxygenase, IPR001006|Procollagen-lysine 5-dioxygenase,
IPR006620|Prolyl 4-hydroxylase, alpha subunit
         (271 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g18210.1 68416.m02316 oxidoreductase, 2OG-Fe(II) oxygenase fa...    41   8e-04
At1g22950.1 68414.m02868 oxidoreductase, 2OG-Fe(II) oxygenase fa...    36   0.022
At3g06280.1 68416.m00721 hypothetical protein                          31   1.1  
At5g46490.2 68418.m05725 disease resistance protein (TIR-NBS cla...    28   7.7  

>At3g18210.1 68416.m02316 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein contains Pfam domain PF03171 2OG-Fe(II)
           oxygenase superfamily
          Length = 394

 Score = 41.1 bits (92), Expect = 8e-04
 Identities = 34/142 (23%), Positives = 62/142 (43%), Gaps = 11/142 (7%)

Query: 84  NFEEDRKHLM--PCTDVYWFPLMSDRFCDEWIAIMEAYGKWSDGTN---NDKRLESGYEA 138
           N EE  ++++  P   V+ F ++   FC+  +A ++ + +W   T          + Y A
Sbjct: 153 NTEESFRNIISEPSPGVFVFDMLQPSFCEMMLAEIDNFERWVGETKFRIMRPNTMNKYGA 212

Query: 139 VPTRDIHMSQVGLERHWLQILKDYVRPLQELVFTGYYHNPPASIMNFVVRYRPDEQPSLR 198
           V      +   GL+    ++++ ++RP+ ++ F+        S   FVV Y  D    L 
Sbjct: 213 V------LDDFGLDTMLDKLMEGFIRPISKVFFSDVGGATLDSHHGFVVEYGKDRDVDLG 266

Query: 199 PHHDSSTYTINLALNTPNVDYE 220
            H D S  T+N+ L    V  E
Sbjct: 267 FHVDDSEVTLNVCLGNQFVGGE 288


>At1g22950.1 68414.m02868 oxidoreductase, 2OG-Fe(II) oxygenase
           family protein contains Pfam domain PF03171 2OG-Fe(II)
           oxygenase superfamily
          Length = 388

 Score = 36.3 bits (80), Expect = 0.022
 Identities = 39/183 (21%), Positives = 72/183 (39%), Gaps = 23/183 (12%)

Query: 98  VYWFPLMSDRFCDEWIAIMEAYGKWS-DGTNNDKRLESGYEAVPTRDIHMSQVGLERHWL 156
           ++ F +   +FC+  +A +E   KW  D  +   R  +    +    + +   G +    
Sbjct: 152 IFTFEMFKPQFCEMLLAEVEHMEKWVYDSRSTIMRPNT----MNNFGVVLDDFGFDSMLQ 207

Query: 157 QILKDYVRPLQELVFTGYYHNPPASIMNFVVRYRPDEQPSLRPHHDSSTYTINLALNTPN 216
           +++ D++ P+ +++F         S   ++V Y  D    L  H D S  ++N+ L    
Sbjct: 208 KLVDDFISPIAQVLFPEVCGTSLDSHHGYIVEYGKDRDVDLGFHVDDSEVSLNVCLGK-- 265

Query: 217 VDYEGGGCRFIRYNCSVR----NTKK---------GWLLMHPGRLTHYHEGLLVTKGTRY 263
             + GG   F    C       +T+K         G  ++H GR  H H     T G R 
Sbjct: 266 -QFSGGELYFRGVRCDKHVNSDSTEKEVYDYSHVPGHAILHRGR--HRHGARATTSGHRA 322

Query: 264 IMI 266
            +I
Sbjct: 323 NLI 325


>At3g06280.1 68416.m00721 hypothetical protein 
          Length = 198

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 1/53 (1%)

Query: 197 LRPHHDSSTYTINLALNTPNVDYEGGGCRFIRYNCSV-RNTKKGWLLMHPGRL 248
           L+ H D S   +N+AL T   D   G C     N  V R + +G+LL + G+L
Sbjct: 29  LKNHGDLSKVALNIALTTLVDDASTGFCIMNLVNLIVLRESSQGYLLTYEGKL 81


>At5g46490.2 68418.m05725 disease resistance protein (TIR-NBS
           class), putative domain signature TIR-NBS exists,
           suggestive of a disease resistance protein.
          Length = 858

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 19/66 (28%), Positives = 29/66 (43%), Gaps = 2/66 (3%)

Query: 22  DMAFCASLRELNIFMYVSNEE--DFGHLVNPETYDITKTHPDMYQLFENNMEWTTRYLHP 79
           +MAFC SL  L     + + +   F      +T+    T+  +  LF  N+E    +LH 
Sbjct: 744 NMAFCNSLETLPTGFNLKSLDRLSFSECTKLKTFPKFSTNISVLNLFGTNIEEYPSHLHL 803

Query: 80  EYLANF 85
           E L  F
Sbjct: 804 ENLVEF 809


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.322    0.138    0.446 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,379,161
Number of Sequences: 28952
Number of extensions: 331116
Number of successful extensions: 620
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 617
Number of HSP's gapped (non-prelim): 4
length of query: 271
length of database: 12,070,560
effective HSP length: 80
effective length of query: 191
effective length of database: 9,754,400
effective search space: 1863090400
effective search space used: 1863090400
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 59 (27.9 bits)