Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000699-TA|BGIBMGA000699-PA|IPR000159|RA,
IPR001849|Pleckstrin-like
(1023 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g37020.1 68414.m04616 Ulp1 protease family protein 38 0.026
At2g30880.2 68415.m03764 pleckstrin homology (PH) domain-contain... 31 3.9
At2g30880.1 68415.m03763 pleckstrin homology (PH) domain-contain... 31 3.9
At4g16120.1 68417.m02443 phytochelatin synthetase-related contai... 30 6.9
At3g48190.1 68416.m05257 ataxia-telangiectasia mutated protein (... 30 6.9
At1g14690.1 68414.m01756 microtubule associated protein (MAP65/A... 30 6.9
At5g45730.1 68418.m05622 DC1 domain-containing protein contains ... 30 9.1
At3g47890.1 68416.m05222 ubiquitin carboxyl-terminal hydrolase-r... 30 9.1
At2g27120.1 68415.m03259 DNA-directed DNA polymerase epsilon cat... 30 9.1
>At1g37020.1 68414.m04616 Ulp1 protease family protein
Length = 611
Score = 38.3 bits (85), Expect = 0.026
Identities = 41/174 (23%), Positives = 68/174 (39%), Gaps = 12/174 (6%)
Query: 210 KSLLVDEKMTCGYVTRLLADKNHVNMEPKWAIVEHLPDLNMERVYED--HEMLVDNLMLW 267
K + EK+ V +L D NM PKW+ + D+ ++ + D H L +N W
Sbjct: 135 KKFIETEKVAHEEVRHMLPDSLE-NMYPKWSDLPENQDMTLDNLIRDIIHNRLRNNA--W 191
Query: 268 TRESKNKILFAERPDKISLFQAPEKFLLNDNDRGRSSEQDDHSRQVMLEEFFGQNGAGAS 327
E P + ++ +ND + ++ H V + + N +
Sbjct: 192 KHEKNGN---ESTPIDDERAELSKRLAAVENDL--KALKESHPADVSINDVTSNNKGDDA 246
Query: 328 TVPSVSGHPVPPMEGPLYLK-NDSKKGWKKIYCVLRPSGLYYSPKDKVKTLKEL 380
T + P E L LK D+ KG KK ++ P PK K+ +KE+
Sbjct: 247 TFNNDKEDETPNKELELQLKIKDATKGKKKKVAIM-PKKASKIPKKKMAKVKEM 299
>At2g30880.2 68415.m03764 pleckstrin homology (PH) domain-containing
protein contains Pfam profile PF00169: PH domain
Length = 381
Score = 31.1 bits (67), Expect = 3.9
Identities = 31/129 (24%), Positives = 56/129 (43%), Gaps = 18/129 (13%)
Query: 340 MEGPLYLKNDSKKGWKKIYCVLRPS--GLYYSPKDKVKTLKELVCLATFDTNEVY-IGLN 396
++GPL+ ++++ + W + + +L P+ + Y + T+K + T + +
Sbjct: 34 LQGPLFKRSETLRKWNERWVILDPTTGKMEYKTRRNEPTIKGTILFDENSTISISPVNFQ 93
Query: 397 WKKKYKSPTDYCFAIKHPRLQQPKSVKFIKFLCAEDQQTMERWMTAMRIAKHGRQL-LEN 455
KY C I P Q K FLCAE + W+T + H QL L+
Sbjct: 94 GLPKYNG---CCIYIGTP---QKKDY----FLCAETPGAAKAWVTTL----HATQLVLKA 139
Query: 456 YRSLVEELT 464
++ VE L+
Sbjct: 140 HKEAVESLS 148
>At2g30880.1 68415.m03763 pleckstrin homology (PH) domain-containing
protein contains Pfam profile PF00169: PH domain
Length = 504
Score = 31.1 bits (67), Expect = 3.9
Identities = 31/129 (24%), Positives = 56/129 (43%), Gaps = 18/129 (13%)
Query: 340 MEGPLYLKNDSKKGWKKIYCVLRPS--GLYYSPKDKVKTLKELVCLATFDTNEVY-IGLN 396
++GPL+ ++++ + W + + +L P+ + Y + T+K + T + +
Sbjct: 34 LQGPLFKRSETLRKWNERWVILDPTTGKMEYKTRRNEPTIKGTILFDENSTISISPVNFQ 93
Query: 397 WKKKYKSPTDYCFAIKHPRLQQPKSVKFIKFLCAEDQQTMERWMTAMRIAKHGRQL-LEN 455
KY C I P Q K FLCAE + W+T + H QL L+
Sbjct: 94 GLPKYNG---CCIYIGTP---QKKDY----FLCAETPGAAKAWVTTL----HATQLVLKA 139
Query: 456 YRSLVEELT 464
++ VE L+
Sbjct: 140 HKEAVESLS 148
>At4g16120.1 68417.m02443 phytochelatin synthetase-related contains
Pfam PF04833: Phytochelatin synthetase-like conserved
region; supporting cDNA gi|26449620|dbj|AK117261.1|
Length = 661
Score = 30.3 bits (65), Expect = 6.9
Identities = 17/70 (24%), Positives = 32/70 (45%)
Query: 438 RWMTAMRIAKHGRQLLENYRSLVEELTQEDLDHLAHARSCSITSIPTKTNNGVTNINSPA 497
R+ + + + HGR L+++R V+ +E L ++A +S+P NG P+
Sbjct: 72 RFESVITVLNHGRDELKSWRVFVKFAHREILVSASNAVLSDGSSLPVSVENGTVFAGYPS 131
Query: 498 QSTSSNMSVA 507
S + A
Sbjct: 132 SDLKSAIQTA 141
>At3g48190.1 68416.m05257 ataxia-telangiectasia mutated protein (Atm)
identical to ataxia-telangiectasia mutated protein (Atm)
[Arabidopsis thaliana] GI:7529272; contains Pfam profile
PF00855: PWWP domain; contains GA donor splice site at
exon 73
Length = 3255
Score = 30.3 bits (65), Expect = 6.9
Identities = 22/82 (26%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
Query: 218 MTCGYVTRLLADKNHVNMEPKWAIVEHLPDLNMERVYEDHEMLVDNLMLWTRESKNKILF 277
+ G+ L K H+ +W + E + D++ ER + E V+ L S +KI
Sbjct: 1404 LCAGFKVSLPLPKEHLPTPSQWDVCEQIDDVDRERNFGLFECSVEALTRICSNS-SKISG 1462
Query: 278 AERPDKISLFQAPEKFLLNDND 299
+ PD + L LL+D D
Sbjct: 1463 CQVPDVVQLPLVLRDPLLHDMD 1484
>At1g14690.1 68414.m01756 microtubule associated protein
(MAP65/ASE1) family protein low similarity to SP|P32380
NUF1 protein (Spindle poly body spacer protein SPC110)
{Saccharomyces cerevisiae}, smooth muscle myosin heavy
chain [Homo sapiens] GI:4417214; contains Pfam profile
PF03999: Microtubule associated protein (MAP65/ASE1
family)
Length = 707
Score = 30.3 bits (65), Expect = 6.9
Identities = 16/62 (25%), Positives = 35/62 (56%)
Query: 15 DPEALLNEWLGELTVLTAGLNSTGDGSLALRPLEIVAPRIDTYRFSMANLEETQDADLDA 74
D +A + + GE++ + LN T GSLAL ++ +++ Y+ + +L++ + L+
Sbjct: 114 DIKAQIEKMSGEISGYSDQLNKTMVGSLALDEQDLTLRKLNEYQTHLRSLQKEKSDRLNK 173
Query: 75 IL 76
+L
Sbjct: 174 VL 175
>At5g45730.1 68418.m05622 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 519
Score = 29.9 bits (64), Expect = 9.1
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Query: 585 DSGTLTRKPRKNRCDDSTLKRHHNIDSTDTSVY 617
D TL RK K+RCDD L HH S++ ++
Sbjct: 422 DCATLPRKV-KHRCDDHVLSLHHGAGSSNGQLW 453
>At3g47890.1 68416.m05222 ubiquitin carboxyl-terminal
hydrolase-related contains Pfam profiles PF00443:
Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
of unknown function (DUF629), PF04781: Protein of unknown
function (DUF627)
Length = 1568
Score = 29.9 bits (64), Expect = 9.1
Identities = 18/41 (43%), Positives = 25/41 (60%), Gaps = 3/41 (7%)
Query: 601 STLKRHHNIDSTDT-SVYGTATTVSITSTESPVRKEPVSPT 640
STL+ HH D S+Y T +S S+E+ RKEPV+P+
Sbjct: 1269 STLEHHHFGDPCVVCSLYAIFTALSTASSET--RKEPVAPS 1307
>At2g27120.1 68415.m03259 DNA-directed DNA polymerase epsilon
catalytic subunit, putative similar to SP|Q07864 DNA
polymerase epsilon, catalytic subunit A (EC 2.7.7.7) (DNA
polymerase II subunit A) {Homo sapiens}; contains Pfam
profiles: PF03175 DNA polymerase type B, organellar and
viral, PF00136 DNA polymerase family B, PF03104 DNA
polymerase family B, exonuclease domain
Length = 2138
Score = 29.9 bits (64), Expect = 9.1
Identities = 9/17 (52%), Positives = 13/17 (76%)
Query: 231 NHVNMEPKWAIVEHLPD 247
N V +EPKW++ HLP+
Sbjct: 1858 NEVTIEPKWSVARHLPE 1874
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.312 0.127 0.365
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 21,474,560
Number of Sequences: 28952
Number of extensions: 874119
Number of successful extensions: 1936
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1929
Number of HSP's gapped (non-prelim): 11
length of query: 1023
length of database: 12,070,560
effective HSP length: 88
effective length of query: 935
effective length of database: 9,522,784
effective search space: 8903803040
effective search space used: 8903803040
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 64 (29.9 bits)
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