SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000699-TA|BGIBMGA000699-PA|IPR000159|RA,
IPR001849|Pleckstrin-like
         (1023 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_6245| Best HMM Match : No HMM Matches (HMM E-Value=.)              141   3e-33
SB_14836| Best HMM Match : No HMM Matches (HMM E-Value=.)              55   3e-07
SB_48713| Best HMM Match : No HMM Matches (HMM E-Value=.)              41   0.006
SB_13705| Best HMM Match : No HMM Matches (HMM E-Value=.)              37   0.095
SB_34168| Best HMM Match : No HMM Matches (HMM E-Value=.)              36   0.17 
SB_53813| Best HMM Match : RA (HMM E-Value=1.1)                        36   0.17 
SB_48451| Best HMM Match : No HMM Matches (HMM E-Value=.)              32   2.7  
SB_35735| Best HMM Match : FF (HMM E-Value=2.29995e-41)                31   4.7  
SB_34373| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   4.7  
SB_31792| Best HMM Match : DUF1373 (HMM E-Value=2.5)                   31   4.7  
SB_37749| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   6.3  
SB_12515| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   6.3  
SB_25304| Best HMM Match : HDV_ag (HMM E-Value=0.55)                   31   6.3  
SB_3976| Best HMM Match : No HMM Matches (HMM E-Value=.)               30   8.3  
SB_55241| Best HMM Match : PH (HMM E-Value=6.1e-12)                    30   8.3  
SB_46064| Best HMM Match : PH (HMM E-Value=1.9e-21)                    30   8.3  
SB_16158| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   8.3  

>SB_6245| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1000

 Score =  141 bits (341), Expect = 3e-33
 Identities = 66/137 (48%), Positives = 96/137 (70%), Gaps = 8/137 (5%)

Query: 337 VPPMEGPLYLKNDSKKGWKKIYCVLRPSGLYYSPKDKVKTLKELVCLATFDTNEVYIG-L 395
           +P ++G LYLK + KK WKK Y  LR SG+YY+PK K K+ ++LVCL  F+   +Y G  
Sbjct: 458 LPELDGVLYLK-EGKKSWKKHYFALRASGIYYTPKGKTKSSRDLVCLIKFEFVNIYYGNA 516

Query: 396 NWKKKYKSPTDYCFAIKHPRLQQPKSVKFIKFLCAEDQQTMERWMTAMRIAKHGRQLLEN 455
           ++KKK+K+PTDYCF +KHP   + +S K IK+ CAED +T  RW+  +++AK+G QLLEN
Sbjct: 517 SFKKKFKAPTDYCFILKHPEYHEVES-KAIKYFCAEDVKTFNRWVVCIKLAKYGYQLLEN 575

Query: 456 YRSLVEELTQEDLDHLA 472
           Y       TQ+++++LA
Sbjct: 576 YSE-----TQDEMENLA 587



 Score =  115 bits (276), Expect = 2e-25
 Identities = 78/315 (24%), Positives = 153/315 (48%), Gaps = 13/315 (4%)

Query: 14  DDPEALLNEWLGELTVLTAGLNSTGDGSLALRPLEIVAPRIDTYRFSMANLEETQDADLD 73
           DD + +LNE  G    L A  ++  D S      E+ +  +D +RFS+  +E++ + DLD
Sbjct: 55  DDIDKMLNELQGFQDEL-AHESAFDDSSSPEAAPELPSKPLDEFRFSLTAMEDSGEVDLD 113

Query: 74  AILGELCALDSEYD----EELSRVSSGFASGSKDRVPRTTEPCTVRQEKDNSDAASGITR 129
           A+L +LC ++ + +    E ++  +         ++     P    Q      A+    R
Sbjct: 114 ALLEDLCVMEKDLNANKTENVNDPTPAMEPTFPVKLNSPISPTAKIQGFGRLFASHEPPR 173

Query: 130 --TESPDNDSAFSDTVXXXXXXXXXXXXXXXXXXXXXXNLHQNQKDASFQQKADKIK--- 184
             T      + F   V                      ++ +  K+ + +++ ++IK   
Sbjct: 174 VTTHGLSGAAFFEIGVFPNFQIRQLLCRISADVRSRLESVQEESKELTPEEQQERIKAEK 233

Query: 185 --LALERMREANIKKLFIKAFS-MDGSSKSLLVDEKMTCGYVTRLLADKNHVNMEPKWAI 241
             +ALE++R A +KKL +K ++  D +SK++ +D+  T   V + +  KN    +P W +
Sbjct: 234 IRIALEKLRAARVKKLVVKVYNDEDPTSKTIAIDQTWTSWEVCKKMMRKNDAEPDPNWVL 293

Query: 242 VEHLPDLNMERVYEDHEMLVDNLMLWTRESKNKILFAERPDKISLFQAPEKFLLNDNDRG 301
           VE LP+L +ER  EDHE +VD +  W  E+ N+I+   + +K +LF+ P+ +LL+ +   
Sbjct: 294 VERLPELCIERSLEDHESVVDVVSSWPWENNNRIVINNKREKYALFKNPQNYLLSSDTSA 353

Query: 302 RSSEQDDHSRQVMLE 316
            +++  + S+ ++L+
Sbjct: 354 GAAQLAEKSKDILLQ 368


>SB_14836| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 92

 Score = 54.8 bits (126), Expect = 3e-07
 Identities = 26/60 (43%), Positives = 40/60 (66%), Gaps = 6/60 (10%)

Query: 413 HPRLQQPKSVKFIKFLCAEDQQTMERWMTAMRIAKHGRQLLENYRSLVEELTQEDLDHLA 472
           HP   + +S K IK+ CAED +T  RW+  +++AK+G QLLENY       TQ+++++LA
Sbjct: 1   HPEYHEVES-KAIKYFCAEDVKTFNRWVVCIKLAKYGYQLLENYSE-----TQDEMENLA 54


>SB_48713| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 663

 Score = 40.7 bits (91), Expect = 0.006
 Identities = 43/165 (26%), Positives = 76/165 (46%), Gaps = 17/165 (10%)

Query: 341 EGPLYLKNDSK-KGWKKIYCVLRPSGLYYSPKDKVKTLKELVCLATFDTNE-------VY 392
           EG L++++  K K WK +  +L+     Y   DK K+  + +     DT++       VY
Sbjct: 263 EGMLHVRDPEKRKDWKPLNVILKNG---YMAVDKSKSFFKSIKSDKSDTDQWSLTNLLVY 319

Query: 393 IGLNWKKKYKSPTDYC-FAIKHPRLQQPKSVKFIKFLCAEDQQTMERWMTA-MRIAKHG- 449
           +G+  +K+ K     C F +    ++   S   I+FLCA+D+  M RW+ A + + ++G 
Sbjct: 320 LGVPAEKQTKGVDTSCGFTVV---VKAEGSGNIIRFLCAKDKDDMYRWVAAILSMKEYGM 376

Query: 450 RQLLENYRSLVEELTQEDLDHLAHARSCSITSIPTKTNNGVTNIN 494
            + LE+   L E  + E     A  R+  +  I    N     +N
Sbjct: 377 TRPLESPEPLFERDSPETEVSTAINRAPGVNQIDPGVNQIDPGVN 421


>SB_13705| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 781

 Score = 36.7 bits (81), Expect = 0.095
 Identities = 15/30 (50%), Positives = 19/30 (63%)

Query: 336 PVPPMEGPLYLKNDSKKGWKKIYCVLRPSG 365
           PV  ++G  +LK    K WKK+YCVLR  G
Sbjct: 133 PVEDVQGKNWLKKSFTKKWKKVYCVLRKYG 162


>SB_34168| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 454

 Score = 35.9 bits (79), Expect = 0.17
 Identities = 19/57 (33%), Positives = 26/57 (45%)

Query: 200 IKAFSMDGSSKSLLVDEKMTCGYVTRLLADKNHVNMEPKWAIVEHLPDLNMERVYED 256
           IK F+ D SS +  V +  T       L   N +  +P W +VEHL DL +     D
Sbjct: 93  IKVFNEDNSSVTFSVGQNTTAMEACYKLMLLNAIKEDPTWVLVEHLTDLGIGEYVTD 149


>SB_53813| Best HMM Match : RA (HMM E-Value=1.1)
          Length = 553

 Score = 35.9 bits (79), Expect = 0.17
 Identities = 19/57 (33%), Positives = 26/57 (45%)

Query: 200 IKAFSMDGSSKSLLVDEKMTCGYVTRLLADKNHVNMEPKWAIVEHLPDLNMERVYED 256
           IK F+ D SS +  V +  T       L   N +  +P W +VEHL DL +     D
Sbjct: 201 IKVFNEDNSSVTFSVGQNTTAMEACYKLMLLNAIKEDPTWVLVEHLTDLGIGEYVTD 257


>SB_48451| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2851

 Score = 31.9 bits (69), Expect = 2.7
 Identities = 15/37 (40%), Positives = 20/37 (54%)

Query: 88  EELSRVSSGFASGSKDRVPRTTEPCTVRQEKDNSDAA 124
           EE +R SS F S  +   PR   P   R + D+SDA+
Sbjct: 632 EERNRESSSFPSDQRPSAPRHDGPLRTRNDSDSSDAS 668


>SB_35735| Best HMM Match : FF (HMM E-Value=2.29995e-41)
          Length = 251

 Score = 31.1 bits (67), Expect = 4.7
 Identities = 21/89 (23%), Positives = 43/89 (48%), Gaps = 4/89 (4%)

Query: 230 KNHVNMEPKWAIVEHLPDLNMERVYEDHEMLVDNLMLWTRESKNKILFAERPDKI-SLFQ 288
           K  +  + +W++VE LP    E+++ DH   +  L    RE   K+L       + S ++
Sbjct: 128 KRTLRKDHRWSMVEPLPKEEREKIFNDH---ISQLHERKREQFRKLLDETTELTLTSSWR 184

Query: 289 APEKFLLNDNDRGRSSEQDDHSRQVMLEE 317
           + +K + +D    + S  D + R+ ++ E
Sbjct: 185 SIKKIIRDDPRYSKFSSHDRNDRRYLILE 213


>SB_34373| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 538

 Score = 31.1 bits (67), Expect = 4.7
 Identities = 17/42 (40%), Positives = 27/42 (64%), Gaps = 2/42 (4%)

Query: 271 SKNKILFAERPDKISLFQAPEKFLLNDNDRGRS--SEQDDHS 310
           S N + F++  DK++LF++ ++ L ND  + RS  SE D HS
Sbjct: 346 SGNMLGFSKPEDKMTLFESIQRSLKNDIQKRRSHQSEGDSHS 387


>SB_31792| Best HMM Match : DUF1373 (HMM E-Value=2.5)
          Length = 696

 Score = 31.1 bits (67), Expect = 4.7
 Identities = 20/61 (32%), Positives = 25/61 (40%)

Query: 309 HSRQVMLEEFFGQNGAGASTVPSVSGHPVPPMEGPLYLKNDSKKGWKKIYCVLRPSGLYY 368
           H RQ ++E     +   AS   S  G  V P  G       S+KG  K Y  LR  G  +
Sbjct: 102 HQRQGVMEALSAAHQMAASQNKSDKGSKVKPQHGHKSQSQRSRKGKTKTYGFLRNHGAKF 161

Query: 369 S 369
           S
Sbjct: 162 S 162


>SB_37749| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1103

 Score = 30.7 bits (66), Expect = 6.3
 Identities = 22/93 (23%), Positives = 40/93 (43%), Gaps = 2/93 (2%)

Query: 817 LVELQTKKLSSPSIQKKVITQTEPTKERTGSIKKVNFADDLPTCSEKKVKKISFNLKEQX 876
           L ++QT   S+P+ + K  ++ E T+E+  S  K    D+    ++K    I  + KE+ 
Sbjct: 237 LKDVQTPAKSAPNKEDK--SKDESTEEKEESATKAEVDDNKKAKADKASISIISDEKEES 294

Query: 877 XXXXXXXXXXXXNESTRLTSPKKLVDSNSNPPK 909
                       +++ +    KK  DS   P K
Sbjct: 295 KDSTKKTDSKKPSDTKKTPDGKKTPDSKKTPAK 327


>SB_12515| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 769

 Score = 30.7 bits (66), Expect = 6.3
 Identities = 25/80 (31%), Positives = 42/80 (52%), Gaps = 3/80 (3%)

Query: 747 QDIVSQLTAQQIEQASRAGQCRVTARTSEPTRSFPRQPSLD--SVHSEASRSSSLPSDKS 804
           QDI++ L    + Q S AG   +  RT   +    RQ ++   +V SEA+   + P+ ++
Sbjct: 20  QDILTGLWNYALVQRSTAGT-PIRNRTDIRSDRRMRQVNVGDPTVPSEATDQPTGPTSEA 78

Query: 805 IYGPQNVAYGACLVELQTKK 824
             GPQ V +G  +V  +TK+
Sbjct: 79  AVGPQLVIWGTDVVVSETKE 98


>SB_25304| Best HMM Match : HDV_ag (HMM E-Value=0.55)
          Length = 2153

 Score = 30.7 bits (66), Expect = 6.3
 Identities = 15/40 (37%), Positives = 23/40 (57%)

Query: 94  SSGFASGSKDRVPRTTEPCTVRQEKDNSDAASGITRTESP 133
           SS     SK+  P+TTE  + +Q K+N D+ +  + T SP
Sbjct: 840 SSATTPPSKNARPKTTESSSTQQFKNNGDSRTSASITPSP 879


>SB_3976| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 730

 Score = 30.3 bits (65), Expect = 8.3
 Identities = 18/65 (27%), Positives = 28/65 (43%)

Query: 410 AIKHPRLQQPKSVKFIKFLCAEDQQTMERWMTAMRIAKHGRQLLENYRSLVEELTQEDLD 469
           A+  P  + P+  K        D Q+  RW   +RI  +  + + N +S V    Q+   
Sbjct: 441 AVITPEEEDPEKKKETPEEPLLDPQSQSRWSRLVRITAYCMRFVSNLKSSVNNPEQKKTS 500

Query: 470 HLAHA 474
           HLA A
Sbjct: 501 HLAPA 505


>SB_55241| Best HMM Match : PH (HMM E-Value=6.1e-12)
          Length = 734

 Score = 30.3 bits (65), Expect = 8.3
 Identities = 13/31 (41%), Positives = 16/31 (51%)

Query: 338 PPMEGPLYLKNDSKKGWKKIYCVLRPSGLYY 368
           P   G L  +    K WK+ YCVL+   LYY
Sbjct: 648 PDCHGYLSKQGHKVKSWKRRYCVLKNGYLYY 678


>SB_46064| Best HMM Match : PH (HMM E-Value=1.9e-21)
          Length = 319

 Score = 30.3 bits (65), Expect = 8.3
 Identities = 42/151 (27%), Positives = 59/151 (39%), Gaps = 15/151 (9%)

Query: 352 KGWKKIYCVLRPSGLYYSPKDKVKTLKELVCLATFDTNEVYIGLNWKKKYKSPTDYCFAI 411
           K WK  Y VL+ +GL Y  K K       +   TF    +YI     KK K    YC  I
Sbjct: 25  KLWKARYFVLQKNGLIYF-KKKSDCAGRPLGAVTFKDISLYIDEIGDKKRK----YCLKI 79

Query: 412 KHPRLQQPKSVKFIKFLCAEDQQTMERWMTAMRIAKHGRQLLENYRSLVEELTQEDLDHL 471
                   K+     FLC   +     W++A+  A     L        EEL +E    L
Sbjct: 80  H----ADGKNY----FLCCFSEDERNAWLSAILTAISSYVLALKEGKTDEELLREPGSSL 131

Query: 472 AHARSCSITSIPTKTNNGVTNINSPAQSTSS 502
              RS S+ ++ T++   +  + S A   SS
Sbjct: 132 --PRSISLEALSTQSIRTLPQMRSRAAFGSS 160


>SB_16158| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 317

 Score = 30.3 bits (65), Expect = 8.3
 Identities = 13/45 (28%), Positives = 26/45 (57%)

Query: 736 EHAMEANSGRVQDIVSQLTAQQIEQASRAGQCRVTARTSEPTRSF 780
           + A++A S RV+ ++ +L AQQ++Q    G+ R+     +  + F
Sbjct: 109 DKAVKAESKRVEALMKKLAAQQVQQEKEEGEKRLEEALIQARKEF 153


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.312    0.127    0.365 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 30,370,802
Number of Sequences: 59808
Number of extensions: 1210050
Number of successful extensions: 3085
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 3065
Number of HSP's gapped (non-prelim): 24
length of query: 1023
length of database: 16,821,457
effective HSP length: 89
effective length of query: 934
effective length of database: 11,498,545
effective search space: 10739641030
effective search space used: 10739641030
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 65 (30.3 bits)

- SilkBase 1999-2023 -