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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000696-TA|BGIBMGA000696-PA|IPR001849|Pleckstrin-like,
IPR001895|Guanine-nucleotide dissociation stimulator CDC25,
IPR008937|Ras guanine nucleotide exchange factor
         (576 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ...   130   4e-31
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc...   113   8e-26
SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha...    29   1.8  
SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase |Schizosacchar...    29   1.8  
SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1...    29   1.8  
SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomy...    29   2.3  
SPCC622.21 |wtf12||wtf element Wtf12|Schizosaccharomyces pombe|c...    29   2.3  
SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm type|Schizos...    29   2.3  
SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po...    27   5.4  
SPBC25B2.07c |mug164||microtubule-associated protein|Schizosacch...    27   5.4  
SPBC83.16c |||conserved fungal protein|Schizosaccharomyces pombe...    27   5.4  
SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy...    27   9.4  
SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual     27   9.4  
SPBC119.04 |mei3||meiosis inducing protein Mei3 |Schizosaccharom...    27   9.4  

>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
           Ste6|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 911

 Score =  130 bits (315), Expect = 4e-31
 Identities = 81/255 (31%), Positives = 132/255 (51%), Gaps = 13/255 (5%)

Query: 87  SINQSWDDIVCS---ALRLSPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLNKLTVAPN 143
           S++ S D I+      L L P ++  QL +L+   F  I   +  T  W  LN+ +    
Sbjct: 644 SLHNSPDPIIYKDELVLLLPPREIAKQLCILEFQSFSHISRIQFLTKIWDNLNRFSPKEK 703

Query: 144 VVAFTKRFNRVSFWTVQEILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHS 203
              F    + V+F T + I+  + P+ R  ++A+FI+V   L ELNN  SLF++ISAL+S
Sbjct: 704 TSTFYLSNHLVNFVT-ETIVQEEEPRRRTNVLAYFIQVCDYLRELNNFASLFSIISALNS 762

Query: 204 ASIYRLTKTWACLSKKDKQQFDKLAELFGETDNWTALREYLKSISLPCIPYLGIFLTDLV 263
           + I+RL KTWA L+ K    F+ L  L     N++  R+ L++  LPC+P+LG++ TDL 
Sbjct: 763 SPIHRLRKTWANLNSKTLASFELLNNLTEARKNFSNYRDCLENCVLPCVPFLGVYFTDLT 822

Query: 264 YIDIAHPAGSPHRV-----TKMNIVLKALERYQSSEYEIAPLPHVANFLNSVRYIEELQK 318
           ++   +     + +     TK+  +L  ++++QS  Y   P+  V   LN V   E    
Sbjct: 823 FLKTGNKDNFQNMINFDKRTKVTRILNEIKKFQSVGYMFNPINEVQELLNEVISRERNTN 882

Query: 319 FVEDDQYKLSLKLEP 333
            +    Y+ SL +EP
Sbjct: 883 NI----YQRSLTVEP 893


>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 987

 Score =  113 bits (271), Expect = 8e-26
 Identities = 71/242 (29%), Positives = 125/242 (51%), Gaps = 16/242 (6%)

Query: 103 SPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLNKLTVAPNVVAFTKRFNRVSFWTVQEI 162
           +P++  +Q+TLL+    K I   E     W   +  +   N + F+  F   ++W +  I
Sbjct: 752 TPEEFASQMTLLEFDYLKQIPSREWIFRSWVSRDSRSAVRNYINFSNCF---TYWIINCI 808

Query: 163 LNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHSASIYRLTKTWACLSKKDKQ 222
           L  ++ KAR  +++ FI+ A K   L N  +L +++SAL+SA IYRL   +  +  +D  
Sbjct: 809 LEKKNTKARTAVISFFIQTAYKCLSLQNFSTLMSIVSALNSAPIYRLHAAYKLVKAEDII 868

Query: 223 QFDKLAELFGETDNWTALREYLKSISLPCIPYLGIFLTDLVYIDIAHP---AGSPHRVT- 278
               L E+     N++  R  L+   LPC+P+LG+ L+DL +ID  +P     SPH ++ 
Sbjct: 869 CLSGLREIVETKKNFSTYRALLRKAELPCVPFLGVILSDLTFIDEGNPDVLDSSPHLLSF 928

Query: 279 ----KMNIVLKALERYQSSEYEIAPLPHVANF-LNSVRYIEELQKFVEDDQYKLSLKLEP 333
               ++  V+  + R+QSS YE+     + ++ L+  R++ +   ++ D     SL LEP
Sbjct: 929 NKRHRLADVVADVCRFQSSSYEMQSNTDLQSYILHRCRFVNQDLSYLFDK----SLSLEP 984

Query: 334 PS 335
            S
Sbjct: 985 RS 986


>SPCC1322.14c |vtc4||vacuolar transporter chaperone
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 721

 Score = 29.1 bits (62), Expect = 1.8
 Identities = 13/52 (25%), Positives = 26/52 (50%)

Query: 520 WADGDAADAFQLINHHSGTVYKFKAGSEYAAKQWIAKIEEVTNKIVKPLPAN 571
           W++ D +D  +L+      VY F+        + I   EE T+++V+ L ++
Sbjct: 38  WSEEDESDFVELLEKELDKVYSFQKNKSAEVMERIRFCEEQTDEVVRRLDSD 89


>SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 413

 Score = 29.1 bits (62), Expect = 1.8
 Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 1/46 (2%)

Query: 38  QSTIPLNSQQSFTKDDCACYYNYGHGENGSNRQHDSIFHYKTNSLP 83
           Q  +P  +Q + T         YGHG N +N+Q++SI H   ++LP
Sbjct: 85  QHNLPPETQYAITHRFDPESSTYGHG-NRTNQQNNSILHPDLHTLP 129


>SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 881

 Score = 29.1 bits (62), Expect = 1.8
 Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 6/48 (12%)

Query: 44  NSQQSFTKDDCACYYNYGHGENGSNRQHDSIFHYKTNSLPAACSINQS 91
           NSQ    +DD +     G   NGS  +++S+ H  TN LP    +NQS
Sbjct: 667 NSQHHHNRDDASS----GLSSNGS--ENESLEHEPTNELPQRPLVNQS 708


>SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 613

 Score = 28.7 bits (61), Expect = 2.3
 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 1/57 (1%)

Query: 402 HTEDKMDEKTPAGSVNLLDDTLLESPSSVACAKMSQSLPHSNIT-KPEDIQCDFQGC 457
           +TED + +    G ++   D         ACA + ++L  +NIT  PE  QC +  C
Sbjct: 531 YTEDMIQQLLTNGLISSTVDNDTYFGQCFACAVVKRTLERNNITASPECQQCYYNYC 587


>SPCC622.21 |wtf12||wtf element Wtf12|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 197

 Score = 28.7 bits (61), Expect = 2.3
 Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 3/46 (6%)

Query: 183 KKLHELNNLHSLFAVISALHSASIYRLTKTWACLSKKDKQQFDKLA 228
           K LH   N++SL  ++ A+ + S+   T  W C++  +K  F K+A
Sbjct: 78  KDLHP--NIYSLLRLLIAVLAVSVVFFT-AWGCVNPLEKSTFGKIA 120


>SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm
           type|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 473

 Score = 28.7 bits (61), Expect = 2.3
 Identities = 17/65 (26%), Positives = 32/65 (49%), Gaps = 3/65 (4%)

Query: 263 VYIDIAHPAGSPHRVTKMNIVLKALERYQSSEYEIA-PLPHVANFLNSVRYIEELQKFVE 321
           + + +A P    H  + +N+V  ++       Y  A P+P  A+  NS  ++  L KFV 
Sbjct: 258 IRVGLATPKSKAHVFSPVNVVPVSMPPV--GFYSAAQPVPQFADTANSTVFVGGLSKFVS 315

Query: 322 DDQYK 326
           +++ K
Sbjct: 316 EEELK 320


>SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1313

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 17/55 (30%), Positives = 26/55 (47%), Gaps = 1/55 (1%)

Query: 310  VRYIEELQKFVEDDQYKLSLKLEPPSPVGSCAGSKESVK-ETVLQGNVTTFNISP 363
            +R    LQ F  D+    SL +   +PV +   S +S+K    L  N+ TF  +P
Sbjct: 1208 IRMNHSLQTFSTDNSGLTSLTVHSHAPVYATGSSNQSIKIWDTLGQNINTFRENP 1262


>SPBC25B2.07c |mug164||microtubule-associated
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 501

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 15/53 (28%), Positives = 28/53 (52%)

Query: 395 SNIFGKSHTEDKMDEKTPAGSVNLLDDTLLESPSSVACAKMSQSLPHSNITKP 447
           SNI  + H+     + T +   N  +++++++  S A  K+S SL   NI+ P
Sbjct: 13  SNILKELHSTSIFTDSTTSQLHNGGENSVMDTEESSAIDKLSASLQQVNISSP 65


>SPBC83.16c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 563

 Score = 27.5 bits (58), Expect = 5.4
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 2/49 (4%)

Query: 205 SIYRLTKTWACLSKKDKQQFDKLAELFGET--DNWTALREYLKSISLPC 251
           S+Y+L K  +  SK +K+ FD  +  F  T   +     EY+++ +L C
Sbjct: 127 SVYKLRKAHSIFSKINKRHFDHFSAAFHSTGRSDVDLANEYVQTGTLLC 175


>SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 589

 Score = 26.6 bits (56), Expect = 9.4
 Identities = 12/41 (29%), Positives = 22/41 (53%), Gaps = 1/41 (2%)

Query: 455 QGCVKRKTILKEGRKISLSSWQRYWLQMSGNMLFFY-GAKS 494
           +G ++   ++ +  K+    W + WL +S N L  Y G+KS
Sbjct: 22  KGIIRSGWVMLKEDKMKYLPWTKKWLVLSSNSLSIYKGSKS 62


>SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1029

 Score = 26.6 bits (56), Expect = 9.4
 Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 1/56 (1%)

Query: 522 DGDAADAFQLINHHSGTVYKFKAGSEYAAKQWIAK-IEEVTNKIVKPLPANLMSFE 576
           D D+A+  +LI     +V   +   E  A   I   I+ +   + KPLP +LMS E
Sbjct: 189 DNDSAEMLRLILKTFKSVIALELPPELLANDMILSWIQLLLAVVQKPLPESLMSLE 244


>SPBC119.04 |mei3||meiosis inducing protein Mei3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 148

 Score = 26.6 bits (56), Expect = 9.4
 Identities = 13/25 (52%), Positives = 17/25 (68%), Gaps = 2/25 (8%)

Query: 102 LSPDDVGNQLTLLDLPCFKAIKPEE 126
           LSP D+ N+LT+LDL  F   +P E
Sbjct: 113 LSPSDLTNELTILDL--FHVPRPNE 135


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.132    0.407 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,836,930
Number of Sequences: 5004
Number of extensions: 126651
Number of successful extensions: 323
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 315
Number of HSP's gapped (non-prelim): 15
length of query: 576
length of database: 2,362,478
effective HSP length: 77
effective length of query: 499
effective length of database: 1,977,170
effective search space: 986607830
effective search space used: 986607830
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 56 (26.6 bits)

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