BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000696-TA|BGIBMGA000696-PA|IPR001849|Pleckstrin-like, IPR001895|Guanine-nucleotide dissociation stimulator CDC25, IPR008937|Ras guanine nucleotide exchange factor (576 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 130 4e-31 SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 113 8e-26 SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccha... 29 1.8 SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase |Schizosacchar... 29 1.8 SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1... 29 1.8 SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomy... 29 2.3 SPCC622.21 |wtf12||wtf element Wtf12|Schizosaccharomyces pombe|c... 29 2.3 SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm type|Schizos... 29 2.3 SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po... 27 5.4 SPBC25B2.07c |mug164||microtubule-associated protein|Schizosacch... 27 5.4 SPBC83.16c |||conserved fungal protein|Schizosaccharomyces pombe... 27 5.4 SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomy... 27 9.4 SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual 27 9.4 SPBC119.04 |mei3||meiosis inducing protein Mei3 |Schizosaccharom... 27 9.4 >SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor Ste6|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 130 bits (315), Expect = 4e-31 Identities = 81/255 (31%), Positives = 132/255 (51%), Gaps = 13/255 (5%) Query: 87 SINQSWDDIVCS---ALRLSPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLNKLTVAPN 143 S++ S D I+ L L P ++ QL +L+ F I + T W LN+ + Sbjct: 644 SLHNSPDPIIYKDELVLLLPPREIAKQLCILEFQSFSHISRIQFLTKIWDNLNRFSPKEK 703 Query: 144 VVAFTKRFNRVSFWTVQEILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHS 203 F + V+F T + I+ + P+ R ++A+FI+V L ELNN SLF++ISAL+S Sbjct: 704 TSTFYLSNHLVNFVT-ETIVQEEEPRRRTNVLAYFIQVCDYLRELNNFASLFSIISALNS 762 Query: 204 ASIYRLTKTWACLSKKDKQQFDKLAELFGETDNWTALREYLKSISLPCIPYLGIFLTDLV 263 + I+RL KTWA L+ K F+ L L N++ R+ L++ LPC+P+LG++ TDL Sbjct: 763 SPIHRLRKTWANLNSKTLASFELLNNLTEARKNFSNYRDCLENCVLPCVPFLGVYFTDLT 822 Query: 264 YIDIAHPAGSPHRV-----TKMNIVLKALERYQSSEYEIAPLPHVANFLNSVRYIEELQK 318 ++ + + + TK+ +L ++++QS Y P+ V LN V E Sbjct: 823 FLKTGNKDNFQNMINFDKRTKVTRILNEIKKFQSVGYMFNPINEVQELLNEVISRERNTN 882 Query: 319 FVEDDQYKLSLKLEP 333 + Y+ SL +EP Sbjct: 883 NI----YQRSLTVEP 893 >SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces pombe|chr 2|||Manual Length = 987 Score = 113 bits (271), Expect = 8e-26 Identities = 71/242 (29%), Positives = 125/242 (51%), Gaps = 16/242 (6%) Query: 103 SPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLNKLTVAPNVVAFTKRFNRVSFWTVQEI 162 +P++ +Q+TLL+ K I E W + + N + F+ F ++W + I Sbjct: 752 TPEEFASQMTLLEFDYLKQIPSREWIFRSWVSRDSRSAVRNYINFSNCF---TYWIINCI 808 Query: 163 LNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHSASIYRLTKTWACLSKKDKQ 222 L ++ KAR +++ FI+ A K L N +L +++SAL+SA IYRL + + +D Sbjct: 809 LEKKNTKARTAVISFFIQTAYKCLSLQNFSTLMSIVSALNSAPIYRLHAAYKLVKAEDII 868 Query: 223 QFDKLAELFGETDNWTALREYLKSISLPCIPYLGIFLTDLVYIDIAHP---AGSPHRVT- 278 L E+ N++ R L+ LPC+P+LG+ L+DL +ID +P SPH ++ Sbjct: 869 CLSGLREIVETKKNFSTYRALLRKAELPCVPFLGVILSDLTFIDEGNPDVLDSSPHLLSF 928 Query: 279 ----KMNIVLKALERYQSSEYEIAPLPHVANF-LNSVRYIEELQKFVEDDQYKLSLKLEP 333 ++ V+ + R+QSS YE+ + ++ L+ R++ + ++ D SL LEP Sbjct: 929 NKRHRLADVVADVCRFQSSSYEMQSNTDLQSYILHRCRFVNQDLSYLFDK----SLSLEP 984 Query: 334 PS 335 S Sbjct: 985 RS 986 >SPCC1322.14c |vtc4||vacuolar transporter chaperone |Schizosaccharomyces pombe|chr 3|||Manual Length = 721 Score = 29.1 bits (62), Expect = 1.8 Identities = 13/52 (25%), Positives = 26/52 (50%) Query: 520 WADGDAADAFQLINHHSGTVYKFKAGSEYAAKQWIAKIEEVTNKIVKPLPAN 571 W++ D +D +L+ VY F+ + I EE T+++V+ L ++ Sbjct: 38 WSEEDESDFVELLEKELDKVYSFQKNKSAEVMERIRFCEEQTDEVVRRLDSD 89 >SPCC576.01c ||SPCPB1C11.04c|sulfonate dioxygenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 413 Score = 29.1 bits (62), Expect = 1.8 Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Query: 38 QSTIPLNSQQSFTKDDCACYYNYGHGENGSNRQHDSIFHYKTNSLP 83 Q +P +Q + T YGHG N +N+Q++SI H ++LP Sbjct: 85 QHNLPPETQYAITHRFDPESSTYGHG-NRTNQQNNSILHPDLHTLP 129 >SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1|||Manual Length = 881 Score = 29.1 bits (62), Expect = 1.8 Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 6/48 (12%) Query: 44 NSQQSFTKDDCACYYNYGHGENGSNRQHDSIFHYKTNSLPAACSINQS 91 NSQ +DD + G NGS +++S+ H TN LP +NQS Sbjct: 667 NSQHHHNRDDASS----GLSSNGS--ENESLEHEPTNELPQRPLVNQS 708 >SPAC1A6.04c |plb1||phospholipase B homolog Plb1|Schizosaccharomyces pombe|chr 1|||Manual Length = 613 Score = 28.7 bits (61), Expect = 2.3 Identities = 17/57 (29%), Positives = 27/57 (47%), Gaps = 1/57 (1%) Query: 402 HTEDKMDEKTPAGSVNLLDDTLLESPSSVACAKMSQSLPHSNIT-KPEDIQCDFQGC 457 +TED + + G ++ D ACA + ++L +NIT PE QC + C Sbjct: 531 YTEDMIQQLLTNGLISSTVDNDTYFGQCFACAVVKRTLERNNITASPECQQCYYNYC 587 >SPCC622.21 |wtf12||wtf element Wtf12|Schizosaccharomyces pombe|chr 3|||Manual Length = 197 Score = 28.7 bits (61), Expect = 2.3 Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 3/46 (6%) Query: 183 KKLHELNNLHSLFAVISALHSASIYRLTKTWACLSKKDKQQFDKLA 228 K LH N++SL ++ A+ + S+ T W C++ +K F K+A Sbjct: 78 KDLHP--NIYSLLRLLIAVLAVSVVFFT-AWGCVNPLEKSTFGKIA 120 >SPBC23E6.01c ||SPBPJ758.01|RNA-binding protein, rrm type|Schizosaccharomyces pombe|chr 2|||Manual Length = 473 Score = 28.7 bits (61), Expect = 2.3 Identities = 17/65 (26%), Positives = 32/65 (49%), Gaps = 3/65 (4%) Query: 263 VYIDIAHPAGSPHRVTKMNIVLKALERYQSSEYEIA-PLPHVANFLNSVRYIEELQKFVE 321 + + +A P H + +N+V ++ Y A P+P A+ NS ++ L KFV Sbjct: 258 IRVGLATPKSKAHVFSPVNVVPVSMPPV--GFYSAAQPVPQFADTANSTVFVGGLSKFVS 315 Query: 322 DDQYK 326 +++ K Sbjct: 316 EEELK 320 >SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1313 Score = 27.5 bits (58), Expect = 5.4 Identities = 17/55 (30%), Positives = 26/55 (47%), Gaps = 1/55 (1%) Query: 310 VRYIEELQKFVEDDQYKLSLKLEPPSPVGSCAGSKESVK-ETVLQGNVTTFNISP 363 +R LQ F D+ SL + +PV + S +S+K L N+ TF +P Sbjct: 1208 IRMNHSLQTFSTDNSGLTSLTVHSHAPVYATGSSNQSIKIWDTLGQNINTFRENP 1262 >SPBC25B2.07c |mug164||microtubule-associated protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 501 Score = 27.5 bits (58), Expect = 5.4 Identities = 15/53 (28%), Positives = 28/53 (52%) Query: 395 SNIFGKSHTEDKMDEKTPAGSVNLLDDTLLESPSSVACAKMSQSLPHSNITKP 447 SNI + H+ + T + N +++++++ S A K+S SL NI+ P Sbjct: 13 SNILKELHSTSIFTDSTTSQLHNGGENSVMDTEESSAIDKLSASLQQVNISSP 65 >SPBC83.16c |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 563 Score = 27.5 bits (58), Expect = 5.4 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 2/49 (4%) Query: 205 SIYRLTKTWACLSKKDKQQFDKLAELFGET--DNWTALREYLKSISLPC 251 S+Y+L K + SK +K+ FD + F T + EY+++ +L C Sbjct: 127 SVYKLRKAHSIFSKINKRHFDHFSAAFHSTGRSDVDLANEYVQTGTLLC 175 >SPAC1F5.09c |shk2|pak2|PAK-related kinase Shk2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 589 Score = 26.6 bits (56), Expect = 9.4 Identities = 12/41 (29%), Positives = 22/41 (53%), Gaps = 1/41 (2%) Query: 455 QGCVKRKTILKEGRKISLSSWQRYWLQMSGNMLFFY-GAKS 494 +G ++ ++ + K+ W + WL +S N L Y G+KS Sbjct: 22 KGIIRSGWVMLKEDKMKYLPWTKKWLVLSSNSLSIYKGSKS 62 >SPCC550.11 |||karyopherin|Schizosaccharomyces pombe|chr 3|||Manual Length = 1029 Score = 26.6 bits (56), Expect = 9.4 Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 1/56 (1%) Query: 522 DGDAADAFQLINHHSGTVYKFKAGSEYAAKQWIAK-IEEVTNKIVKPLPANLMSFE 576 D D+A+ +LI +V + E A I I+ + + KPLP +LMS E Sbjct: 189 DNDSAEMLRLILKTFKSVIALELPPELLANDMILSWIQLLLAVVQKPLPESLMSLE 244 >SPBC119.04 |mei3||meiosis inducing protein Mei3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 26.6 bits (56), Expect = 9.4 Identities = 13/25 (52%), Positives = 17/25 (68%), Gaps = 2/25 (8%) Query: 102 LSPDDVGNQLTLLDLPCFKAIKPEE 126 LSP D+ N+LT+LDL F +P E Sbjct: 113 LSPSDLTNELTILDL--FHVPRPNE 135 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.132 0.407 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,836,930 Number of Sequences: 5004 Number of extensions: 126651 Number of successful extensions: 323 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 315 Number of HSP's gapped (non-prelim): 15 length of query: 576 length of database: 2,362,478 effective HSP length: 77 effective length of query: 499 effective length of database: 1,977,170 effective search space: 986607830 effective search space used: 986607830 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 56 (26.6 bits)
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