Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000696-TA|BGIBMGA000696-PA|IPR001849|Pleckstrin-like,
IPR001895|Guanine-nucleotide dissociation stimulator CDC25,
IPR008937|Ras guanine nucleotide exchange factor
(576 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_5795| Best HMM Match : RasGEF (HMM E-Value=5.3e-06) 101 2e-21
SB_29257| Best HMM Match : No HMM Matches (HMM E-Value=.) 77 3e-14
SB_2866| Best HMM Match : RasGEF (HMM E-Value=1.3e-26) 70 6e-12
SB_48676| Best HMM Match : No HMM Matches (HMM E-Value=.) 69 8e-12
SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24) 67 3e-11
SB_54230| Best HMM Match : EGF (HMM E-Value=0) 67 4e-11
SB_54793| Best HMM Match : No HMM Matches (HMM E-Value=.) 63 7e-10
SB_10256| Best HMM Match : RasGEF (HMM E-Value=1.8e-34) 60 4e-09
SB_49937| Best HMM Match : No HMM Matches (HMM E-Value=.) 58 3e-08
SB_23730| Best HMM Match : No HMM Matches (HMM E-Value=.) 57 3e-08
SB_25073| Best HMM Match : Extensin_2 (HMM E-Value=0.27) 46 6e-05
SB_8431| Best HMM Match : Spt4 (HMM E-Value=4.6) 42 0.002
SB_57255| Best HMM Match : Atrophin-1 (HMM E-Value=0.91) 41 0.003
SB_41416| Best HMM Match : RasGEF (HMM E-Value=1e-04) 38 0.022
SB_42044| Best HMM Match : ubiquitin (HMM E-Value=1.2e-06) 37 0.038
SB_33848| Best HMM Match : RasGEF_N (HMM E-Value=2.3e-13) 35 0.16
SB_7844| Best HMM Match : RasGEF (HMM E-Value=0.00058) 33 0.47
SB_19200| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.47
SB_6176| Best HMM Match : Bromo_TP (HMM E-Value=1.5e-23) 30 5.8
>SB_5795| Best HMM Match : RasGEF (HMM E-Value=5.3e-06)
Length = 155
Score = 101 bits (242), Expect = 2e-21
Identities = 45/83 (54%), Positives = 61/83 (73%)
Query: 154 VSFWTVQEILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHSASIYRLTKTW 213
VSFW V+EILN + K R +M+HFI++AK+L+E+NNLHSL AVI+ L SA +YRL +TW
Sbjct: 13 VSFWVVREILNAKKLKTRVAVMSHFIRIAKRLYEMNNLHSLKAVIAGLQSAPVYRLNQTW 72
Query: 214 ACLSKKDKQQFDKLAELFGETDN 236
LS++D F+KL +L E N
Sbjct: 73 EQLSRRDHTIFEKLEDLLSEDQN 95
>SB_29257| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 494
Score = 77.4 bits (182), Expect = 3e-14
Identities = 37/115 (32%), Positives = 65/115 (56%), Gaps = 1/115 (0%)
Query: 101 RLSPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLN-KLTVAPNVVAFTKRFNRVSFWTV 159
+L P ++ QLTLL+ ++A++P EL WTK + K +PN++ N+++ W
Sbjct: 124 KLHPIEIARQLTLLESELYRAVRPSELVGSVWTKEDIKHLTSPNLLKMIHHTNKITVWFE 183
Query: 160 QEILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHSASIYRLTKTWA 214
+ I+ + + R ++ I + ELNN + + V+SAL+SA I+RL T+A
Sbjct: 184 KSIVEMSNLEERVAVLTRIIDILTVFQELNNFNGILEVVSALNSAPIFRLQHTFA 238
>SB_2866| Best HMM Match : RasGEF (HMM E-Value=1.3e-26)
Length = 566
Score = 69.7 bits (163), Expect = 6e-12
Identities = 53/215 (24%), Positives = 99/215 (46%), Gaps = 18/215 (8%)
Query: 140 VAPNVVAFTKRFNRVSFWTVQEILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVIS 199
++ N+ RFN V +W + EI R ++ FIK+A + NL+S FA+I
Sbjct: 297 ISANLDICLSRFNEVHYWVITEICLATQLSKRVFLLKKFIKIASYCKDYKNLNSAFAIIM 356
Query: 200 ALHSASIYRLTKTWACLSKKDKQQFDKLAELFGETDNWTALREYLKSISLPCIPYLG-IF 258
AL+ A+I RLT+TW + K K+ + +L + + N R Y + + I Y F
Sbjct: 357 ALNCAAISRLTQTWEKIPNKYKKLYRELDAILDPSGNH---RVYRLVVDITIINYGNKTF 413
Query: 259 LTDLVYIDIAHPAGSPHRVTKMNIVLKALERYQSSEYEIAPLPHVANFL-----NSVRYI 313
+ +LV + ++ + I L+ +E +S+ + + P+ H A + + R+
Sbjct: 414 VNELVNFE---------KMRMITISLRLVEYCRSAPFAVHPVCHDAVSVRLGSGTAARFF 464
Query: 314 EELQKFVEDDQYKLSLKLEPPSPVGSCAGSKESVK 348
+ + + D +S+K + + + G VK
Sbjct: 465 DAMNTVITDSSIGVSIKTVDVNSLAAACGIAFIVK 499
>SB_48676| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 453
Score = 69.3 bits (162), Expect = 8e-12
Identities = 43/167 (25%), Positives = 85/167 (50%), Gaps = 4/167 (2%)
Query: 102 LSPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLNKLTVAPNVVAFTKRFNRVSFWTVQE 161
++P + ++T++D AI EE+T ++K+ +P+VV +N ++ T E
Sbjct: 75 IAPRAMAEEITMIDSEMLSAIGYEEVTGGALMSIDKVK-SPHVVQMIDFYNSIAAITATE 133
Query: 162 ILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHSASIYRLTKTWACLSKKDK 221
+L + + RA +++ I++A + + NN +S A++S L +I RLTKTW + +K K
Sbjct: 134 VLVKEGSRDRAYVISRVIQMADECVDFNNFNSAKALMSGLRYVAITRLTKTWKKVPEKHK 193
Query: 222 QQFDKLAELFGETDNWTALREYLKSISLP---CIPYLGIFLTDLVYI 265
+ L+ + D+ + ++ P + +LG L + YI
Sbjct: 194 RTLRHLSIITTSADDIAIFYRHRRACQNPSSSSVHFLGDILGQMAYI 240
>SB_45114| Best HMM Match : RasGEF (HMM E-Value=3.7e-24)
Length = 315
Score = 67.3 bits (157), Expect = 3e-11
Identities = 62/244 (25%), Positives = 109/244 (44%), Gaps = 22/244 (9%)
Query: 101 RLSPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLNKLTVAPNVVAFTKRFNRVSFWTVQ 160
++S + QLT ++ F + E W K +K APN++ ++RFN S
Sbjct: 67 KISSSQLAEQLTFVEHLLFSRVPAHEFLNLAWMKQDKHIRAPNILRVSQRFNETSALVTS 126
Query: 161 EILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHSASIYRLTKTWACLSKKD 220
EIL QSP ARA ++ + VA+ L+N +S+ + A S S R + + K+
Sbjct: 127 EILKRQSPSARATVIEKWASVAEVCRNLHNFNSVLEITPARKSKSSKR-----SLVLKEG 181
Query: 221 KQQFDKLA-ELFGETDNWTALREYLKSISLPCIPYLGIF----LTDLVYIDIAHPAGSPH 275
+ + L+ E F + + + K +L C + ++ DL +I+ P +
Sbjct: 182 SRTCETLSDECFAIRKDIFVVVQSFKEWTL-CFEAMEMYPEDQNKDLAFIEDGTPDCNED 240
Query: 276 ---RVTKMNI---VLKALERYQSSEYEIAPLPHVANFLNSVRYIEELQKFVEDDQYKLSL 329
+KM + V++ + YQ + Y I V ++L + + + ED Y+ SL
Sbjct: 241 GLVNFSKMRMIAQVIQEIRHYQQTPYAIQGDRKVIHYLTAKELLMD-----EDALYRTSL 295
Query: 330 KLEP 333
LEP
Sbjct: 296 LLEP 299
>SB_54230| Best HMM Match : EGF (HMM E-Value=0)
Length = 1359
Score = 66.9 bits (156), Expect = 4e-11
Identities = 45/143 (31%), Positives = 78/143 (54%), Gaps = 7/143 (4%)
Query: 100 LRLSPDDVGNQLTLLDLPCFKAIKPEELT---TCGWTKLNKLTVAPNVVA--FTKRFNRV 154
L P ++ Q+TLLD F A+ +++ G + L P ++ RFN++
Sbjct: 360 LLFHPKELARQITLLDHEFFCAVTSDDIQKKIAMGTGRRKNLDSQPRLLCERVADRFNQL 419
Query: 155 SFWTVQEILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFA-VISALHSASIYRLTKTW 213
S W V IL +S + RA++ +FI+ AK+ +L N +++ A V++AL S+SI RL +T
Sbjct: 420 STWVVASILTEESVEKRAQMFINFIETAKQCLKLRNFNAVMAIVVAALGSSSIRRLARTK 479
Query: 214 ACLSKKDKQQFDKLAELFGETDN 236
+ K+ +Q K+ E+ +T N
Sbjct: 480 QLVPKEFLEQCAKI-EMLMDTKN 501
Score = 31.9 bits (69), Expect = 1.4
Identities = 12/36 (33%), Positives = 21/36 (58%)
Query: 235 DNWTALREYLKSISLPCIPYLGIFLTDLVYIDIAHP 270
+N+ R L++ P +PY GI++ DL +I +P
Sbjct: 542 NNYKKYRTTLRASPTPAVPYFGIYMKDLTFIAEGNP 577
>SB_54793| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 523
Score = 62.9 bits (146), Expect = 7e-10
Identities = 30/80 (37%), Positives = 50/80 (62%)
Query: 134 KLNKLTVAPNVVAFTKRFNRVSFWTVQEILNGQSPKARAEIMAHFIKVAKKLHELNNLHS 193
K+ K +P+V+ + FNR++ EIL ++ +ARA +++ I++A K L N +S
Sbjct: 66 KVEKYVRSPDVMEMVEFFNRIAMLVASEILAEETTQARARVVSKVIQIADKCRLLGNYNS 125
Query: 194 LFAVISALHSASIYRLTKTW 213
L A+++ L SA IYRL +TW
Sbjct: 126 LKALMAGLQSAPIYRLKQTW 145
>SB_10256| Best HMM Match : RasGEF (HMM E-Value=1.8e-34)
Length = 792
Score = 60.5 bits (140), Expect = 4e-09
Identities = 37/102 (36%), Positives = 53/102 (51%), Gaps = 3/102 (2%)
Query: 146 AFTKRFNRVSFWTVQEILN---GQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALH 202
A +FN VS + IL +SP R + +I +A+ L N SL A+ISAL
Sbjct: 37 ATVDQFNAVSLKVLSSILRTPENKSPAQRGRYINQWINIAQCCRNLKNFSSLKAIISALQ 96
Query: 203 SASIYRLTKTWACLSKKDKQQFDKLAELFGETDNWTALREYL 244
SASI+RL K+W + ++ F++LA L E N R+ L
Sbjct: 97 SASIHRLKKSWQHVPRESTALFNELASLCSEEKNQQMSRDVL 138
>SB_49937| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 850
Score = 57.6 bits (133), Expect = 3e-08
Identities = 36/121 (29%), Positives = 57/121 (47%), Gaps = 12/121 (9%)
Query: 115 DLPCFKAIKPEELTTCGWTKLNKLTVAPNVVAFTKRFNRVSFWTVQEILNGQSPKARAEI 174
D F+AI P E W + + PN+ F + NR +FW V EI + R ++
Sbjct: 715 DFELFRAIDPREYIYDVWEFGPEFS--PNLAKFEETVNRETFWVVTEICGESNVVKRMKL 772
Query: 175 MAHFIKVAKKL----------HELNNLHSLFAVISALHSASIYRLTKTWACLSKKDKQQF 224
+ HFIK+A L + N++S FA+IS L ++ RL TW L + + +
Sbjct: 773 IKHFIKIASMLKTWSFVVQYCKDCKNINSAFAIISGLGHTAVKRLKNTWEKLPNRYYKMY 832
Query: 225 D 225
+
Sbjct: 833 E 833
>SB_23730| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 76
Score = 57.2 bits (132), Expect = 3e-08
Identities = 34/86 (39%), Positives = 50/86 (58%), Gaps = 11/86 (12%)
Query: 310 VRYIEELQKFVEDDQYKLSLKLEPPSPVGSCAGSKESVKETVLQGNVTTFNISPSHKLGS 369
+ YIEELQKF+ED+ Y+LSL++EP S V S + V + + +++T+ +SP
Sbjct: 1 MNYIEELQKFLEDNNYRLSLQIEPKS-VKSSGEATSGVNLSRSRESLSTYPVSPK----- 54
Query: 370 GSLRLQGTSHQGKFIPTHRKCRSLGS 395
TS KF+P HRK SLG+
Sbjct: 55 -----ASTSQVSKFVPGHRKAYSLGT 75
>SB_25073| Best HMM Match : Extensin_2 (HMM E-Value=0.27)
Length = 744
Score = 46.4 bits (105), Expect = 6e-05
Identities = 24/79 (30%), Positives = 45/79 (56%)
Query: 102 LSPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLNKLTVAPNVVAFTKRFNRVSFWTVQE 161
++P +LT++D +AI+ +E+ W +++ +P+V A + +NR+S V
Sbjct: 666 VNPSAFAEELTMIDSEMLRAIEFDEIKNGAWREIDVEQRSPHVHAMIEFYNRLSAICVAN 725
Query: 162 ILNGQSPKARAEIMAHFIK 180
IL+ +S K RA+I+ IK
Sbjct: 726 ILSKESAKDRAKIITKIIK 744
>SB_8431| Best HMM Match : Spt4 (HMM E-Value=4.6)
Length = 62
Score = 41.5 bits (93), Expect = 0.002
Identities = 23/61 (37%), Positives = 36/61 (59%)
Query: 162 ILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHSASIYRLTKTWACLSKKDK 221
+LN S + RA+ + F KVA+ L EL N + L AV L+++ + RL +T +SK K
Sbjct: 2 VLNHTSAEDRAKCIEKFQKVAQSLKELQNFNGLLAVTGGLNNSVLSRLLQTREYVSKDCK 61
Query: 222 Q 222
+
Sbjct: 62 E 62
>SB_57255| Best HMM Match : Atrophin-1 (HMM E-Value=0.91)
Length = 1249
Score = 40.7 bits (91), Expect = 0.003
Identities = 23/87 (26%), Positives = 44/87 (50%)
Query: 94 DIVCSALRLSPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLNKLTVAPNVVAFTKRFNR 153
+++ S + P + +LT++D + I+ +E+ W +P+V A + +N
Sbjct: 1163 NVLLSLSVIDPSALAEELTMIDSEMLREIEFDEIKGGVWRTSEMEQRSPHVHAMIQFYNN 1222
Query: 154 VSFWTVQEILNGQSPKARAEIMAHFIK 180
+S V EIL+ + K RA ++A IK
Sbjct: 1223 LSAICVTEILSKEKTKDRAHVIAKIIK 1249
>SB_41416| Best HMM Match : RasGEF (HMM E-Value=1e-04)
Length = 141
Score = 37.9 bits (84), Expect = 0.022
Identities = 36/134 (26%), Positives = 64/134 (47%), Gaps = 25/134 (18%)
Query: 187 ELNNLHSLFA-VISALHSASIYRLTKTWACLSKKDKQQFDKLAELF------------GE 233
+L N +++ A V++AL S+SI RL +T + K+ +Q K+ L GE
Sbjct: 4 KLRNFNAVMAIVVAALGSSSIRRLARTKQLVPKEFLEQCAKIEMLMDTKVPALFGLVVGE 63
Query: 234 ------TDNWTALREYLKSISLPCIPYLGIFLTDLVYIDIAHP---AGSPHRVTK---MN 281
+N+ R L++ P +PY GI++ DL +I +P G ++K +
Sbjct: 64 YLVDVYANNYKKYRTTLRASPTPAVPYFGIYMKDLTFIAEGNPDFLKGGLVTISKRRQVY 123
Query: 282 IVLKALERYQSSEY 295
IV+ + ++Q Y
Sbjct: 124 IVINEIRQFQKKSY 137
>SB_42044| Best HMM Match : ubiquitin (HMM E-Value=1.2e-06)
Length = 1425
Score = 37.1 bits (82), Expect = 0.038
Identities = 19/72 (26%), Positives = 36/72 (50%), Gaps = 1/72 (1%)
Query: 146 AFTKRFNRVSFWTVQEILNGQSPKARAEIMAHFIKVAKKLHELN-NLHSLFAVISALHSA 204
A +R+ +++W ++ R +++ I++A L + + NL S AV+ L
Sbjct: 737 ALIERYTNLTYWVGALVVAAGDLGPRTQVVTKLIELADVLSDKHGNLVSFMAVLEGLALP 796
Query: 205 SIYRLTKTWACL 216
+ RL TW+CL
Sbjct: 797 QVTRLHHTWSCL 808
>SB_33848| Best HMM Match : RasGEF_N (HMM E-Value=2.3e-13)
Length = 899
Score = 35.1 bits (77), Expect = 0.16
Identities = 15/52 (28%), Positives = 26/52 (50%)
Query: 101 RLSPDDVGNQLTLLDLPCFKAIKPEELTTCGWTKLNKLTVAPNVVAFTKRFN 152
++S + QLT ++ F + E W K +K APN++ ++RFN
Sbjct: 847 KISSSQLAEQLTFVEHLLFSRVPAHEFLNLAWMKQDKHIRAPNILRVSQRFN 898
>SB_7844| Best HMM Match : RasGEF (HMM E-Value=0.00058)
Length = 1299
Score = 33.5 bits (73), Expect = 0.47
Identities = 24/89 (26%), Positives = 41/89 (46%), Gaps = 3/89 (3%)
Query: 181 VAKKLHELNNLHSLFAVISALHSASIYRLTKTWACLSKKDKQQFDKLAELFGETDNWTAL 240
+A+K +NN +S A++S+ I RL+ TW +S K K L D+ +
Sbjct: 954 IAEKCALMNNFNSCKALLSSFRFHVISRLSDTWKKVSTKAKSTKAHLLAQMSSLDDIAIM 1013
Query: 241 REYLK---SISLPCIPYLGIFLTDLVYID 266
R + + + I +LG L + YI+
Sbjct: 1014 RRHQRVCLKRGVNSIQFLGDILHQVHYIE 1042
>SB_19200| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 852
Score = 33.5 bits (73), Expect = 0.47
Identities = 21/72 (29%), Positives = 35/72 (48%)
Query: 149 KRFNRVSFWTVQEILNGQSPKARAEIMAHFIKVAKKLHELNNLHSLFAVISALHSASIYR 208
+RF + E+ N S ++E+ A K + NN+ + V S+++ +I R
Sbjct: 652 QRFLKPLSAAYHEVANSYSSNNQSELQAVVTKHQETFTRDNNMGLVKQVCSSVYKKNILR 711
Query: 209 LTKTWACLSKKD 220
LTKT+ LS D
Sbjct: 712 LTKTFLTLSLSD 723
>SB_6176| Best HMM Match : Bromo_TP (HMM E-Value=1.5e-23)
Length = 684
Score = 29.9 bits (64), Expect = 5.8
Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Query: 326 KLSLKLEPPSPVGSCAGSKESVKETVLQGNVTTFNISPSHKLGSGSLRLQGTS 378
++ LKLEPPS A +KE+ K+ Q V N P K S + L G++
Sbjct: 527 RIKLKLEPPSTTDMLATTKEA-KQPTAQKIVIHTNKEPKIKKTSSHVGLPGST 578
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.132 0.407
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,612,420
Number of Sequences: 59808
Number of extensions: 891639
Number of successful extensions: 1853
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1829
Number of HSP's gapped (non-prelim): 22
length of query: 576
length of database: 16,821,457
effective HSP length: 86
effective length of query: 490
effective length of database: 11,677,969
effective search space: 5722204810
effective search space used: 5722204810
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 63 (29.5 bits)
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