GeneModel BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000696-TA|BGIBMGA000696-PA|IPR001849|Pleckstrin-like,
IPR001895|Guanine-nucleotide dissociation stimulator CDC25,
IPR008937|Ras guanine nucleotide exchange factor
         (576 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g43470.1 68415.m05402 expressed protein                             32   0.90 
At1g65130.1 68414.m07384 ubiquitin carboxyl-terminal hydrolase-r...    31   2.8  
At1g14185.1 68414.m01678 glucose-methanol-choline (GMC) oxidored...    30   4.8  
At5g61940.1 68418.m07775 ubiquitin carboxyl-terminal hydrolase-r...    29   6.4  
At1g62110.1 68414.m07008 mitochondrial transcription termination...    29   6.4  
At1g58025.1 68414.m06576 DNA-binding bromodomain-containing prot...    29   6.4  

>At2g43470.1 68415.m05402 expressed protein
          Length = 498

 Score = 32.3 bits (70), Expect = 0.90
 Identities = 25/96 (26%), Positives = 44/96 (45%), Gaps = 5/96 (5%)

Query: 180 KVAKKLHELNNLHSLFAVISALHSASIYRLTKTWACLSKKDKQQFDKLAELFGETDNWTA 239
           K ++ ++EL+   S + ++ A  S  ++  TKT     ++  Q F KL +   E  N   
Sbjct: 279 KGSELVNELHQYASFYELLKAESSEKVFPGTKT----VEEGMQMFRKLYDTDQENFNGVV 334

Query: 240 LREYLKSISLPCIPYLGIFLTDLVYIDIAHPAGSPH 275
                KS++ PC+  L   L+ L Y  + +  G  H
Sbjct: 335 AIHLSKSVAQPCVA-LAHILSGLSYTGVQNLLGLSH 369


>At1g65130.1 68414.m07384 ubiquitin carboxyl-terminal
           hydrolase-related contains Pfam profiles PF00443:
           Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
           of unknown function (DUF629), PF04781: Protein of
           unknown function (DUF627)
          Length = 1086

 Score = 30.7 bits (66), Expect = 2.8
 Identities = 23/84 (27%), Positives = 38/84 (45%), Gaps = 1/84 (1%)

Query: 492 AKSFKGTNRSDFRSERCKVLTLVGWIAVWADGDAADAFQLINHHSGTVYKFKAGSEYAAK 551
           A  FK ++  D      KV      +  W   DAA A ++I +    V  F   + ++ K
Sbjct: 254 AAEFKPSSTKDMPQTVTKVWGHKISVGGWDPVDAAAAVEMIKNRLQDVKAFAYENGWS-K 312

Query: 552 QWIAKIEEVTNKIVKPLPANLMSF 575
            W   I+E  +K++K +   L+SF
Sbjct: 313 DWPLAIDEERSKLLKEIKLLLVSF 336


>At1g14185.1 68414.m01678 glucose-methanol-choline (GMC)
           oxidoreductase family protein similar to mandelonitrile
           lyase from Prunus serotina [SP|P52706, SP|P52707];
           contains Pfam profile PF00732 GMC oxidoreductase
          Length = 503

 Score = 29.9 bits (64), Expect = 4.8
 Identities = 18/60 (30%), Positives = 30/60 (50%), Gaps = 4/60 (6%)

Query: 323 DQYKLSLKLEPPSPVGSCAGSKESVKETVLQGNVTTFNISPSHKL----GSGSLRLQGTS 378
           D++  +L ++PP  V    G K  ++  VL  N+TT   + S K+      G L+L  T+
Sbjct: 317 DRFSQNLTVDPPQVVAITEGFKFILQSLVLPTNITTTRTAISAKIAFPKSKGRLKLNNTN 376


>At5g61940.1 68418.m07775 ubiquitin carboxyl-terminal
           hydrolase-related contains Pfam profiles PF00443:
           Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
           of unknown function (DUF629), PF04781: Protein of
           unknown function (DUF627)
          Length = 1094

 Score = 29.5 bits (63), Expect = 6.4
 Identities = 15/60 (25%), Positives = 31/60 (51%), Gaps = 1/60 (1%)

Query: 517 IAVWADGDAADAFQLINHHSGTVYKFKAGSEYA-AKQWIAKIEEVTNKIVKPLPANLMSF 575
           +  W   DA  A ++I +    V  F + ++   +K+W   ++E  +K++K +   L+SF
Sbjct: 302 VGSWEPVDAVAATEMIKNQLADVKAFASKAKNGWSKEWPLAVDEERSKLLKEIKLLLVSF 361


>At1g62110.1 68414.m07008 mitochondrial transcription termination
           factor family protein / mTERF family protein contains
           Pfam profile PF02536: mTERF
          Length = 462

 Score = 29.5 bits (63), Expect = 6.4
 Identities = 21/76 (27%), Positives = 34/76 (44%), Gaps = 2/76 (2%)

Query: 281 NIVLKALERYQSSEYEIA--PLPHVANFLNSVRYIEELQKFVEDDQYKLSLKLEPPSPVG 338
           + V + +E  +SS+YE    PLP      N +R +  L+      +   SL +    PV 
Sbjct: 161 DFVKEIIEADKSSKYETLCQPLPEANRQGNKIRNVSVLRDLGVPQKLLFSLLISDAQPVC 220

Query: 339 SCAGSKESVKETVLQG 354
                +ES+K+ V  G
Sbjct: 221 GKENFEESLKKVVEMG 236


>At1g58025.1 68414.m06576 DNA-binding bromodomain-containing protein
           contains bromodomain, INTERPRO:IPR001487; contains
           prenyl group binding site (CAAX box) Prosite:PS00294
          Length = 769

 Score = 29.5 bits (63), Expect = 6.4
 Identities = 21/72 (29%), Positives = 31/72 (43%), Gaps = 3/72 (4%)

Query: 401 SHTEDKMDEKTP---AGSVNLLDDTLLESPSSVACAKMSQSLPHSNITKPEDIQCDFQGC 457
           S  E KM+   P   AGSV+L D+   +S + V     +   P  N T  +DI       
Sbjct: 47  SELESKMEVDAPSPIAGSVDLADNIAAKSVARVKVKLKTSKAPEPNETLRDDIDKSSSQA 106

Query: 458 VKRKTILKEGRK 469
           V  K ++   +K
Sbjct: 107 VLEKPVVPVEKK 118


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.132    0.407 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,724,057
Number of Sequences: 28952
Number of extensions: 651940
Number of successful extensions: 1551
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1551
Number of HSP's gapped (non-prelim): 6
length of query: 576
length of database: 12,070,560
effective HSP length: 85
effective length of query: 491
effective length of database: 9,609,640
effective search space: 4718333240
effective search space used: 4718333240
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 62 (29.1 bits)