Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000686-TA|BGIBMGA000686-PA|IPR005399|KCNAB voltage-gated
K+ channel, beta subunit, IPR001395|Aldo/keto reductase
(529 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC965.06 |||potassium channel subunit |Schizosaccharomyces pom... 134 4e-32
SPAC977.14c |||aldo/keto reductase, unknown biological role|Schi... 81 3e-16
SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase YakC|Schizo... 46 1e-05
SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism... 46 1e-05
SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyc... 43 9e-05
SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde reduc... 43 9e-05
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 39 0.002
SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyc... 35 0.032
SPBC215.11c |||aldo/keto reductase, unknown biological role|Schi... 34 0.056
SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|c... 32 0.17
SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 31 0.30
SPAC9E9.11 |plr1|plr|pyridoxal reductase |Schizosaccharomyces po... 29 1.2
SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces pom... 28 3.7
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 27 4.9
>SPCC965.06 |||potassium channel subunit |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 344
Score = 134 bits (323), Expect = 4e-32
Identities = 72/207 (34%), Positives = 112/207 (54%), Gaps = 9/207 (4%)
Query: 104 YKNLGKSGLRVPNIGLGLWTMLSEESIE-----DVVMTALENGINLFDLSEAHC-AKGEA 157
++ LG+SGL+V LG W E + + + A + GIN FD +E + E
Sbjct: 15 FRFLGRSGLKVSAFSLGGWLTYGNEGYDVEHTKNCLKQAWDLGINTFDTAEIYSNGNSET 74
Query: 158 ELGRILKKRNVRRTSIIITTKIYWSTKFHTNNRSDERGLSRKHVIESVKASLGRLQVEYI 217
+G+ +K+ R+ +ITTK+++ N + GLSRKH+IE + ASL RL + Y+
Sbjct: 75 VMGKAIKELGWDRSEYVITTKVFFGAGTKLPNTT---GLSRKHIIEGLNASLKRLGLPYV 131
Query: 218 DVVLLHKVDPVCPMEELVRVMNYVINEGWVMYWGTARWSPSEIMDAYTNCRQFNCITPIV 277
DV++ H+ DP PMEE+VR +I +G YWGT+ WS EI A+ ++N I P+
Sbjct: 132 DVIMAHRPDPSVPMEEVVRAFTQLIQDGKAFYWGTSEWSAFEIEHAHHIATKYNLIAPVA 191
Query: 278 EQTEYHMFCREKPELYMPELYHKIGVG 304
+Q +Y+ R+ E + L G G
Sbjct: 192 DQPQYNYLTRDHFEKDLLPLQQIYGYG 218
Score = 56.4 bits (130), Expect = 9e-09
Identities = 29/85 (34%), Positives = 50/85 (58%)
Query: 407 QVTGEEPRTYGDKLRDLANLANSLNCSMSQLAVAWCLKNESVNCLLLGATSVEQFKENIH 466
Q+ E +T D++R ++ +A + + SQLA+AW LKN V+ +LGA+ EQ EN+
Sbjct: 254 QLQTPEGKTQLDQVRQISKIAEQIGATPSQLALAWTLKNPYVSTTILGASKPEQIVENVK 313
Query: 467 ALQVYIKKIFKVCVKSFKLLQQSPL 491
A++ K ++ K ++L +PL
Sbjct: 314 AVEFIDKLTPEILKKIDEILNFTPL 338
>SPAC977.14c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 1|||Manual
Length = 351
Score = 81.4 bits (192), Expect = 3e-16
Identities = 66/240 (27%), Positives = 114/240 (47%), Gaps = 32/240 (13%)
Query: 96 SAVTPGLRYKNLGKSGLRVPNIGLGL-----------WTMLSEESIEDVVMTALENGINL 144
S+++P L Y LG SGL+V + LG W + EE + ++ A + GI
Sbjct: 2 SSLSPEL-YGCLGNSGLKVSKLILGCMSYGKKEYWEDWVLEDEEEVFKIMKAAYDAGIRT 60
Query: 145 FDLSEAHCAKGEAEL-GRILKKRNVRRTSIIITTKIYWSTK-----------------FH 186
FD + + A EL G+ ++K + R+SI+I +K ++ +
Sbjct: 61 FDTANCYSAGVSEELVGKFIRKYEIPRSSIVILSKCFFPVRKDLIKIFGDLSSRGVHFLD 120
Query: 187 TNNRSDERGLSRKHVIESVKASLGRLQVEYIDVVLLHKVDPVCPMEELVRVMNYVINEGW 246
+ +++ GLSRKH+ ++V+ S+ RL YIDV+ +H+ DP EE++R +N V+ G
Sbjct: 121 SPELANQCGLSRKHIFDAVEDSVKRLGT-YIDVLQIHRYDPHVSAEEVMRALNDVVESGK 179
Query: 247 VMYWGTARWSPSEIMDAYTNCRQFNCITPIVEQTEYHMFCREKPELYMPELYHKIGVGKI 306
V Y G + + ++ + I Q +++ RE+ +P K GVG I
Sbjct: 180 VRYIGASTMRCYQFIELQNTAEKHGWHKFISMQNYHNLLYREEEREMIP-YCQKTGVGLI 238
>SPAC1F7.12 |yak3|yakC, SPAC21E11.01|aldose reductase
YakC|Schizosaccharomyces pombe|chr 1|||Manual
Length = 340
Score = 46.4 bits (105), Expect = 1e-05
Identities = 50/191 (26%), Positives = 89/191 (46%), Gaps = 24/191 (12%)
Query: 114 VPNIG---LGLWTML---SEESIEDVVMTALENGINLFDLSEAHCAKGEAE-LGRILKKR 166
VP IG +GL M SEE+ + V+ A + G +D S+ + E +GR K+
Sbjct: 13 VPAIGFGCMGLHAMYGPSSEEANQAVLTHAADLGCTFWDSSDMYGFGANEECIGRWFKQT 72
Query: 167 NVRRTSIIITTKIYWSTKFHTNNRSDERGLSRK--HVIESVKASLGRLQVEYIDVVLLHK 224
RR I + TK + N + E L+ + ++ +++ SL RL ++ ID+ +H+
Sbjct: 73 G-RRKEIFLATKF----GYEKNPETGELSLNNEPDYIEKALDLSLKRLGIDCIDLYYVHR 127
Query: 225 VDPVCPMEELVRVMNYVINEGWVMYWGTARWSPSEIMDAYTNCRQFNCITPI-VEQTEYH 283
P+E+++ + + G + Y G + S + I R+ + P+ Q EY
Sbjct: 128 FSGETPIEKIMGALKKCVEAGKIRYIGLSECSANTI-------RRAAAVYPVSAVQVEYS 180
Query: 284 MFCR--EKPEL 292
F E+PE+
Sbjct: 181 PFSLEIERPEI 191
>SPBC8E4.04 |||aldo/keto reductase involved in pentose catabolism
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 325
Score = 46.4 bits (105), Expect = 1e-05
Identities = 36/114 (31%), Positives = 59/114 (51%), Gaps = 15/114 (13%)
Query: 110 SGLRVPNIGLGLWTMLSEESIEDVVMTALENGINLFDLSEAHCAKGEAELGRILKKRNVR 169
+G ++P+IGLG W +E+ ++ V AL+ G D AH E E+G +++ V
Sbjct: 19 NGDKIPSIGLGTWRSGKDET-KNAVCAALKAGYRHIDT--AHIYGNEKEIGEGIRESGVP 75
Query: 170 RTSIIITTKIYWSTKFHTNNRSDERGLSRKHVIESVKASLGRLQVEYIDVVLLH 223
RT I +T+K+ W + GL V +++ +L L +EYID L+H
Sbjct: 76 RTDIWVTSKL-WC-------NAHRAGL----VPLALEKTLQDLNLEYIDAYLIH 117
>SPAC2F3.05c |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 275
Score = 43.2 bits (97), Expect = 9e-05
Identities = 49/184 (26%), Positives = 80/184 (43%), Gaps = 25/184 (13%)
Query: 110 SGLRVPNIGLGLWTMLSEESIEDVVMTALENGINLFDLSEAHCAKGEAELGR-ILK---K 165
+GL+ P G + M++ D V AL+ G D ++ + EA+ GR ILK +
Sbjct: 10 NGLKCPQFAYGSY-MVNRTKCFDSVYAALQCGYRHIDSAQMY--HNEADCGRAILKFMEE 66
Query: 166 RNVRRTSIIITTKIYWSTKFHTNNRSDERGLSRKHVIESVKASLGRLQVEYIDVVLLHKV 225
+R I T+K+ +D G K + S+ AS+ + YID+ LLH
Sbjct: 67 TGTKREDIWFTSKL-----------NDLSGY--KSTLSSIDASVKACGLGYIDLFLLH-- 111
Query: 226 DPVCPMEELVRVMNYVINEGWVMYWGTARWSPSEIMDAYTNCRQFNCITPIVEQTEYHMF 285
P E + + + EG + G + + P I + + + I P V Q E H F
Sbjct: 112 SPYGDRIESWKALEKGVEEGKLRAIGVSNFGPHHIQELLDSHPK---IIPCVNQIELHPF 168
Query: 286 CREK 289
C ++
Sbjct: 169 CSQQ 172
>SPAC19G12.09 |||NADH/NADPH dependent indole-3-acetaldehyde
reductase AKR3C2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 284
Score = 43.2 bits (97), Expect = 9e-05
Identities = 47/179 (26%), Positives = 78/179 (43%), Gaps = 30/179 (16%)
Query: 114 VPNIGLGLWTMLSEE-----SIEDVVMTALENGINLFDLSEAHCAKGEAELGRILKKRNV 168
VP G+G E+ +I D V AL G D +E + E E+G LK+ NV
Sbjct: 12 VPAYGVGTALFKKEKGEINRTIVDSVKNALAAGFIHIDCAEVY--GNEEEVGVALKEANV 69
Query: 169 RRTSIIITTKIYWSTKFHTNNRSDERGLSRKHVIESVKASLGRLQVEYIDVVLLHKVDPV 228
R+ + IT+K+ + ++ E++ SL +L +Y+D+ LLH P
Sbjct: 70 PRSKLFITSKVMHNVD---------------NIPEALNESLRKLGTDYLDLYLLHSPIPF 114
Query: 229 ----CPMEELVRVMNYVINEGWVMYWGTARWSPSEIMDAYTNCRQFNCITPIVEQTEYH 283
P+ E + M + G V G + + ++ + + + ITP V Q E+H
Sbjct: 115 YEKKIPISEGWKAMETALGTGLVHSVGVSNFRIPDLEELL----KTSTITPRVNQIEFH 169
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 38.7 bits (86), Expect = 0.002
Identities = 30/113 (26%), Positives = 58/113 (51%), Gaps = 15/113 (13%)
Query: 111 GLRVPNIGLGLWTMLSEESIEDVVMTALENGINLFDLSEAHCAKGEAELGRILKKRNVRR 170
G ++P +GLG W ++ ++ V TAL+ G D + + E E+G +K+ V R
Sbjct: 21 GSKIPGLGLGTWRSEPNQT-KNAVKTALQYGYRHIDAAAIY--GNEDEVGDGIKESGVPR 77
Query: 171 TSIIITTKIYWSTKFHTNNRSDERGLSRKHVIESVKASLGRLQVEYIDVVLLH 223
I +T+K++ N + E V ++++ +L L+++Y+D L+H
Sbjct: 78 KDIWVTSKLW------CNAHAPEA------VPKALEKTLKDLKLDYLDEYLIH 118
>SPAP32A8.02 |||xylose and arabinose reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 283
Score = 34.7 bits (76), Expect = 0.032
Identities = 50/220 (22%), Positives = 93/220 (42%), Gaps = 31/220 (14%)
Query: 110 SGLRVPNIGLGLWTMLSEESIEDVVMTALENGINLFDLSEAHCAKGEAELGRIL----KK 165
+G+ +P IG G + ML +V AL++G D + + E G+ + +K
Sbjct: 15 NGMVIPRIGFGAF-MLKYNECYGLVTQALDSGYRHIDTAAVY--GNEDICGKAIVDWCEK 71
Query: 166 RNVRRTSIIITTKIYWSTKFHTNNRSDERGLSRKHVIESVKASLGRLQVEYIDVVLLHKV 225
NV+RT I +T+K+ + +++ ++++SL L YID+ L+
Sbjct: 72 NNVKRTDIFLTSKLANCSDYYSTR-------------AAIRSSLHHLGT-YIDLFLIQ-- 115
Query: 226 DPVCPMEELV---RVMNYVINEGWVMYWGTARWSPSEIMDAYTNCRQFNCITPIVEQTEY 282
P + + + M ++ G + G + + + + Y + +F P V Q E
Sbjct: 116 SPAGGKKSRIASWKAMEEFVDSGDIRSVGVSNYGVKHLQELYASNPKF---YPCVNQIEL 172
Query: 283 HMFCREKPELYMPELYHKIGVGKIYILFF-IQLNYRSLVP 321
H F + ++ H I + L I+LN LVP
Sbjct: 173 HPFL-SQDDIVKYCQSHDIAIEAYSPLTHGIRLNDEKLVP 211
>SPBC215.11c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 33.9 bits (74), Expect = 0.056
Identities = 18/65 (27%), Positives = 35/65 (53%)
Query: 199 KHVIESVKASLGRLQVEYIDVVLLHKVDPVCPMEELVRVMNYVINEGWVMYWGTARWSPS 258
K + + V S+ RL V+ ID+ LH++DP P ++ + + EG + + G + +
Sbjct: 113 KFLRQEVLMSMRRLGVKQIDLWQLHRIDPKVPRKDQFSEIAAMKKEGLIRHVGLSEVTVD 172
Query: 259 EIMDA 263
+I +A
Sbjct: 173 DIKEA 177
Score = 31.1 bits (67), Expect = 0.40
Identities = 13/47 (27%), Positives = 27/47 (57%)
Query: 426 LANSLNCSMSQLAVAWCLKNESVNCLLLGATSVEQFKENIHALQVYI 472
++ L+ S SQ+A++W L+ V + G + V+ +EN+ A + +
Sbjct: 232 VSKDLDRSTSQIALSWVLQRSPVMLPIPGTSKVDHLEENVKAAGIQL 278
>SPAC3A11.11c |||pyridoxal reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 334
Score = 32.3 bits (70), Expect = 0.17
Identities = 47/194 (24%), Positives = 83/194 (42%), Gaps = 15/194 (7%)
Query: 117 IGLGL----WTM--LSEESIEDVVMTALENGINLFDLSEAHCAKGEAELGRILKKRNVRR 170
IGLGL WT + +E ++ AL +G + +D E + E L R ++
Sbjct: 12 IGLGLKSLTWTENPVPDEEAFRIMNYALSHGCSFWDAGEFY-GLSEPLANLQLLSRYFQK 70
Query: 171 TSIIITTKIYWSTKFHTNNRSDERGLSRKHVIESVKASLGRLQ-VEYIDVVLLHKVDPVC 229
I K++ S K + + +R+ + +S+K L+ V+ ID+ +DP
Sbjct: 71 FPDSID-KVFLSVKGAFDPETHRVHGTRECITKSIKTVRETLKKVKTIDLYQCAAIDPDT 129
Query: 230 PMEELVRVMNYVINEGWVMYWGTARWSPSEIMDAYTNCRQFNCITPIVEQTEYHMFCREK 289
P+EE + + ++ G + G S EI A++ R I I + Y M RE
Sbjct: 130 PIEETMACLKEFVDSGDIRCIGLCEPSVEEIKRAHSVVR----IAAI--EVHYSMLFREI 183
Query: 290 PELYMPELYHKIGV 303
+ +L H + +
Sbjct: 184 EYNGVKKLCHDLSI 197
>SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1496
Score = 31.5 bits (68), Expect = 0.30
Identities = 19/83 (22%), Positives = 41/83 (49%), Gaps = 3/83 (3%)
Query: 19 SESTVSRCTPHTPLGLPDSASVDSN--PMPTIYRCRAPIASLDC-MDDFSANSQQMLDQA 75
S S+ + HTP+ + S+ ++ N + T+ + I+S +DD + S++ Q
Sbjct: 575 SSSSPRHFSSHTPVNVDKSSYLEMNGHSLSTVISGKESISSFGAKVDDLKSTSKESARQF 634
Query: 76 GEVTVTRHMSTYMGNHTAMPSAV 98
G+ +T + GN + + S++
Sbjct: 635 GKSALTERLQKLAGNSSNVSSSL 657
>SPAC9E9.11 |plr1|plr|pyridoxal reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 333
Score = 29.5 bits (63), Expect = 1.2
Identities = 41/187 (21%), Positives = 77/187 (41%), Gaps = 17/187 (9%)
Query: 110 SGLRVPNIGLGL----WT--MLSEESIEDVVMTALENGINLFDLSEAHCAKGEAELGRIL 163
SG +V IG GL W +E +V+ AL G N +D E + +L
Sbjct: 5 SGFKVGPIGFGLMGLTWKPKQTPDEEAFEVMNYALSQGSNYWDAGEFYGVDPPTSNLDLL 64
Query: 164 KKRNVRRTSIIITTKIYWSTKFHTNNRSDERGLSRKHVIESVKASLGRLQ-VEYIDVVLL 222
+ + K++ S K + ++ + V +SV+ + L+ + +D+
Sbjct: 65 ARYFEKYPEN--ANKVFLSVKGGLDFKTLVPDGNPDFVSKSVENVIAHLRGTKKLDLFQC 122
Query: 223 HKVDPVCPMEELVRVMNYVINEGWVMYWGTARWSPSEIMDAYTNCRQFNCITPIVE-QTE 281
+VDP P+E ++ + ++ G + G + S I A+ + PI + E
Sbjct: 123 ARVDPNVPIETTMKTLKGFVDSGKISCVGLSEVSAETIKRAH-------AVVPIAAVEVE 175
Query: 282 YHMFCRE 288
Y +F R+
Sbjct: 176 YSLFSRD 182
>SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 558
Score = 27.9 bits (59), Expect = 3.7
Identities = 18/71 (25%), Positives = 34/71 (47%), Gaps = 3/71 (4%)
Query: 369 EEITGLFAKSRFNRKYSTFSWCE---EDLAVPEMRSSGSKEQVTGEEPRTYGDKLRDLAN 425
EE+ L KS + + + E E LA PE+ + SKE+ + R + + R +
Sbjct: 175 EEVESLDVKSEKDTESNNLGQNELLKEQLATPEVETHRSKEETILHKDRLFDYQNRSYMH 234
Query: 426 LANSLNCSMSQ 436
+ N + ++S+
Sbjct: 235 VPNDVGINLSE 245
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 27.5 bits (58), Expect = 4.9
Identities = 16/44 (36%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
Query: 163 LKKRNVRRTSIIITTKIYWSTKFHTNNRSDERGLSRKHVIESVK 206
LK+RN+ + I T WS K H ++ ++E L R+ ++ES+K
Sbjct: 557 LKQRNIPLANGSIFTAQEWSEKLHLSS-TNEVLLFRRLLVESLK 599
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.320 0.135 0.409
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,418,652
Number of Sequences: 5004
Number of extensions: 101642
Number of successful extensions: 234
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 214
Number of HSP's gapped (non-prelim): 20
length of query: 529
length of database: 2,362,478
effective HSP length: 76
effective length of query: 453
effective length of database: 1,982,174
effective search space: 897924822
effective search space used: 897924822
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 56 (26.6 bits)
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