Silkworm BLAST Result against tribolium

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000684-TA|BGIBMGA000684-PA|IPR001611|Leucine-rich
repeat, IPR003591|Leucine-rich repeat, typical subtype
         (563 letters)

Database: tribolium 
           317 sequences; 114,650 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF322227-1|AAK01654.1|  782|Tribolium castaneum cell surface pro...   109   4e-26
AM292367-1|CAL23179.2| 1451|Tribolium castaneum gustatory recept...    28   0.15 
AM292324-1|CAL23136.1|  398|Tribolium castaneum gustatory recept...    28   0.15 
AY008296-1|AAG22858.1|  556|Tribolium castaneum Dorsal protein.        25   1.8  
DQ855492-1|ABH88179.1|  251|Tribolium castaneum chemosensory pro...    23   7.4  
AY884065-1|AAX84206.1|  697|Tribolium castaneum laccase 1 protein.     22   9.8  

>AF322227-1|AAK01654.1|  782|Tribolium castaneum cell surface
           protein chaoptin protein.
          Length = 782

 Score =  109 bits (263), Expect = 4e-26
 Identities = 102/368 (27%), Positives = 170/368 (46%), Gaps = 24/368 (6%)

Query: 84  NLRELHIVNSNVPSIGQFSFWG--LQNLRVLNLTHNNLTSVVADNFRGLINLTELHLDHN 141
           +L+++H+ ++ +  I + +F G   ++L  +  + N++ +V    F  LI L ++HLD N
Sbjct: 67  SLKKVHLQDNTIEMIHRGTFQGDIHRDLTEVYFSFNSVRNVQQHTFADLIQLEQIHLDDN 126

Query: 142 HIEQMSSETFRHLTSLKTLILANNQISTLVSRLFRMLAKLSILDLSDNPLTDLNPEVFKD 201
            IE +    F +L SLK L L  N+I+T+    F+ L +L  LDL+ N ++ L+  +F  
Sbjct: 127 RIESLERRAFMNLKSLKRLNLKGNKIATIAYETFQNLPELEDLDLAYNSISSLDFNIFDQ 186

Query: 202 IQHLRIFKCRRCLLRRINTQIYHLVPRLEELDLG--ENL------FKYLTS--DEFISLK 251
           +  L +F       + IN  +   VP  ++  LG  +N+      F  +TS   +F    
Sbjct: 187 VGSLGMFHVNMSHNKLINLVVAPSVPFEQDTGLGGLQNIKVLDLSFNNITSVAKQFFRPV 246

Query: 252 RLK--KLKLDGNQLSVIVDNMFGRNRELHVLTLSRNRXXXXXXXXXXXXXXXXXXDISHN 309
            L   +L L  N+L     ++FG    L VL LS N                   D SHN
Sbjct: 247 ELSLMQLYLGHNKLLNATKDLFGNMPHLQVLDLSHNSLYELDFDTFRNTKKLQWLDTSHN 306

Query: 310 KIDRFHLQTFAPVVDSLKTINFSGN---NLPLNEIAIV-LQILPEIHGVGLANLSLQEIP 365
           +I       F   + +L+ ++FS N   +LP N      L+ L   H + L  L L  + 
Sbjct: 307 RISEIPNDLFR-FLGNLRIVDFSHNRLRSLPDNLFRETGLERLDVSHNL-LGKLPLTSLS 364

Query: 366 QNFFTYNEHLVALDLSWNKLSRFPF-KLLSKTKFLQRLDISHNKLQTLSEQDLQRLEAIA 424
                  + L  LDLSWN +S       L++ K L  LD+S+N+L  +     + +  +A
Sbjct: 365 ---LASAQTLSELDLSWNSISSLSHGGQLARFKCLSWLDLSYNRLGQIDAGTFKGIPRLA 421

Query: 425 EVNLMKNN 432
            +NL  N+
Sbjct: 422 SLNLGHNS 429



 Score = 84.2 bits (199), Expect = 2e-18
 Identities = 81/334 (24%), Positives = 145/334 (43%), Gaps = 16/334 (4%)

Query: 83  VNLRELHIVNSNVPSIGQFSFWGLQNLRVLNLTHNNLTSVVADNFRGLINLTELHLDHNH 142
           + L+  H ++S+V +    +   L  L  L+L++N L +V  ++F  L +L ++HL  N 
Sbjct: 18  LTLKLTHALSSSVQNFPSDAIKILNRLEELDLSNNRLRNVPDNSFHFLRSLKKVHLQDNT 77

Query: 143 IEQMSSETFRH--LTSLKTLILANNQISTLVSRLFRMLAKLSILDLSDNPLTDLNPEVFK 200
           IE +   TF+      L  +  + N +  +    F  L +L  + L DN +  L    F 
Sbjct: 78  IEMIHRGTFQGDIHRDLTEVYFSFNSVRNVQQHTFADLIQLEQIHLDDNRIESLERRAFM 137

Query: 201 DIQHLRIFKCRRCLLRRINTQIYHLVPRLEELDLGENLFKYLTSDEFISLKRLKKLKLDG 260
           +++ L+    +   +  I  + +  +P LE+LDL  N    L  + F  +          
Sbjct: 138 NLKSLKRLNLKGNKIATIAYETFQNLPELEDLDLAYNSISSLDFNIFDQV---------- 187

Query: 261 NQLSVIVDNMFGRNRELHVLTLSRNRXXXXXXXXXXXXXXXXXXDISHNKIDRFHLQTFA 320
             L +   NM   N+ ++++                        D+S N I     Q F 
Sbjct: 188 GSLGMFHVNM-SHNKLINLVVAP--SVPFEQDTGLGGLQNIKVLDLSFNNITSVAKQFFR 244

Query: 321 PVVDSLKTINFSGNNLPLNEIAIVLQILPEIHGVGLANLSLQEIPQNFFTYNEHLVALDL 380
           PV  SL  + + G+N  LN    +   +P +  + L++ SL E+  + F   + L  LD 
Sbjct: 245 PVELSLMQL-YLGHNKLLNATKDLFGNMPHLQVLDLSHNSLYELDFDTFRNTKKLQWLDT 303

Query: 381 SWNKLSRFPFKLLSKTKFLQRLDISHNKLQTLSE 414
           S N++S  P  L      L+ +D SHN+L++L +
Sbjct: 304 SHNRISEIPNDLFRFLGNLRIVDFSHNRLRSLPD 337



 Score = 82.6 bits (195), Expect = 7e-18
 Identities = 71/295 (24%), Positives = 123/295 (41%), Gaps = 12/295 (4%)

Query: 130 LINLTELHLDHNHIEQMSSETFRHLTSLKTLILANNQISTLVSRLFRMLAKLSILDLSDN 189
           L+ L   H   + ++   S+  + L  L+ L L+NN++  +    F  L  L  + L DN
Sbjct: 17  LLTLKLTHALSSSVQNFPSDAIKILNRLEELDLSNNRLRNVPDNSFHFLRSLKKVHLQDN 76

Query: 190 PLTDLNPEVFKDIQHLRIFKCRRCL--LRRINTQIYHLVPRLEELDLGENLFKYLTSDEF 247
            +  ++   F+   H  + +       +R +    +  + +LE++ L +N  + L    F
Sbjct: 77  TIEMIHRGTFQGDIHRDLTEVYFSFNSVRNVQQHTFADLIQLEQIHLDDNRIESLERRAF 136

Query: 248 ISLKRLKKLKLDGNQLSVIVDNMFGRNRELHVLTLSRNRXXX--XXXXXXXXXXXXXXXD 305
           ++LK LK+L L GN+++ I    F    EL  L L+ N                     +
Sbjct: 137 MNLKSLKRLNLKGNKIATIAYETFQNLPELEDLDLAYNSISSLDFNIFDQVGSLGMFHVN 196

Query: 306 ISHNKIDR--------FHLQTFAPVVDSLKTINFSGNNLPLNEIAIVLQILPEIHGVGLA 357
           +SHNK+          F   T    + ++K ++ S NN+          +   +  + L 
Sbjct: 197 MSHNKLINLVVAPSVPFEQDTGLGGLQNIKVLDLSFNNITSVAKQFFRPVELSLMQLYLG 256

Query: 358 NLSLQEIPQNFFTYNEHLVALDLSWNKLSRFPFKLLSKTKFLQRLDISHNKLQTL 412
           +  L    ++ F    HL  LDLS N L    F     TK LQ LD SHN++  +
Sbjct: 257 HNKLLNATKDLFGNMPHLQVLDLSHNSLYELDFDTFRNTKKLQWLDTSHNRISEI 311



 Score = 78.2 bits (184), Expect = 1e-16
 Identities = 64/271 (23%), Positives = 128/271 (47%), Gaps = 16/271 (5%)

Query: 80  APFVNLRELHIVNSNVPSIGQFSFWGLQNLRVLNLTHNNLTSVVADNFRGLINLTELHLD 139
           A  + L ++H+ ++ + S+ + +F  L++L+ LNL  N + ++  + F+ L  L +L L 
Sbjct: 113 ADLIQLEQIHLDDNRIESLERRAFMNLKSLKRLNLKGNKIATIAYETFQNLPELEDLDLA 172

Query: 140 HNHIEQMSSETFRHLTSLKT--LILANNQISTLV---SRLFRM------LAKLSILDLSD 188
           +N I  +    F  + SL    + +++N++  LV   S  F        L  + +LDLS 
Sbjct: 173 YNSISSLDFNIFDQVGSLGMFHVNMSHNKLINLVVAPSVPFEQDTGLGGLQNIKVLDLSF 232

Query: 189 NPLTDLNPEVFK--DIQHLRIFKCRRCLLRRINTQIYHLVPRLEELDLGENLFKYLTSDE 246
           N +T +  + F+  ++  ++++     LL      ++  +P L+ LDL  N    L  D 
Sbjct: 233 NNITSVAKQFFRPVELSLMQLYLGHNKLL-NATKDLFGNMPHLQVLDLSHNSLYELDFDT 291

Query: 247 FISLKRLKKLKLDGNQLSVIVDNMFGRNRELHVLTLSRNRXXXXXXXXXXXXXXXXXXDI 306
           F + K+L+ L    N++S I +++F     L ++  S NR                  D+
Sbjct: 292 FRNTKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNR-LRSLPDNLFRETGLERLDV 350

Query: 307 SHNKIDRFHLQTFA-PVVDSLKTINFSGNNL 336
           SHN + +  L + +     +L  ++ S N++
Sbjct: 351 SHNLLGKLPLTSLSLASAQTLSELDLSWNSI 381



 Score = 66.1 bits (154), Expect = 6e-13
 Identities = 71/295 (24%), Positives = 133/295 (45%), Gaps = 14/295 (4%)

Query: 68  NFLTIGPIFTQPAPFVNLRELHIVNSNVPSIGQFSFWGLQNLRVLNLTHNNLTSVVADNF 127
           N  ++   F +P   ++L +L++ ++ + +  +  F  + +L+VL+L+HN+L  +  D F
Sbjct: 234 NITSVAKQFFRPVE-LSLMQLYLGHNKLLNATKDLFGNMPHLQVLDLSHNSLYELDFDTF 292

Query: 128 RGLINLTELHLDHNHIEQMSSETFRHLTSLKTLILANNQISTLVSRLFRMLAKLSILDLS 187
           R    L  L   HN I ++ ++ FR L +L+ +  ++N++ +L   LFR    L  LD+S
Sbjct: 293 RNTKKLQWLDTSHNRISEIPNDLFRFLGNLRIVDFSHNRLRSLPDNLFRETG-LERLDVS 351

Query: 188 DN-----PLTDLNPEVFKDIQHLRIFKCRRCLLRRINTQIYHLVPRLEELDLGENLFKYL 242
            N     PLT L+    + +  L +       L     Q+      L  LDL  N    +
Sbjct: 352 HNLLGKLPLTSLSLASAQTLSELDLSWNSISSLSH-GGQLARF-KCLSWLDLSYNRLGQI 409

Query: 243 TSDEFISLKRLKKLKLDGN-QLSVIVDNMFGRNRELHVLTLSRNRXXXXXXXXXXXXXXX 301
            +  F  + RL  L L  N QL++ ++ +  +  E  +L L+ +                
Sbjct: 410 DAGTFKGIPRLASLNLGHNSQLTLEINGLSFQGLEYTLLHLNLDN-VSLSQVPALSTPNL 468

Query: 302 XXXDISHNKIDRFHLQTFAPVVDSLKTINFSGNNLPLNEIAIVLQILPEIHGVGL 356
               ++ N +    L+  A  + SL+ +N   N+  L+ + IV   L E+  + L
Sbjct: 469 LSLSLAFNSLPTVALEV-AGNISSLRYLNLDYND--LSAVPIVTHSLTELRHLSL 520



 Score = 48.0 bits (109), Expect = 2e-07
 Identities = 51/185 (27%), Positives = 87/185 (47%), Gaps = 8/185 (4%)

Query: 78  QPAPFVNLRELHIVNSNVPSIGQFSFWGLQNLRVLNLTHNNLTSVVAD--NFRGL-INLT 134
           Q A F  L  L +  + +  I   +F G+  L  LNL HN+  ++  +  +F+GL   L 
Sbjct: 389 QLARFKCLSWLDLSYNRLGQIDAGTFKGIPRLASLNLGHNSQLTLEINGLSFQGLEYTLL 448

Query: 135 ELHLDHNHIEQMSSETFRHLTSLKTLILANNQISTLVSRLFRMLAKLSILDLSDNPLTDL 194
            L+LD+  + Q+ + +  +L SL    LA N + T+   +   ++ L  L+L  N L+ +
Sbjct: 449 HLNLDNVSLSQVPALSTPNLLSLS---LAFNSLPTVALEVAGNISSLRYLNLDYNDLSAV 505

Query: 195 NPEVFKDIQHLRIFKCRRCLLRRI-NTQIYHLVPRLEELDLGENLFKYLTSDEFISLKRL 253
            P V   +  LR        +  + NT +     +LEEL+L       L S  F  ++ L
Sbjct: 506 -PIVTHSLTELRHLSLEGNPITTLSNTSLLGAANQLEELNLKNIDLTVLESGAFCKMQPL 564

Query: 254 KKLKL 258
           + LK+
Sbjct: 565 RTLKI 569


>AM292367-1|CAL23179.2| 1451|Tribolium castaneum gustatory receptor
           candidate 46 protein.
          Length = 1451

 Score = 28.3 bits (60), Expect = 0.15
 Identities = 16/56 (28%), Positives = 29/56 (51%), Gaps = 1/56 (1%)

Query: 126 NFRGLINLTELHLDHNHIEQMSSETFRHLTSLKTLILANNQISTLVSRLFRMLAKL 181
           N R  I+ +++   H H+ + S + F  +  +  L+L  N   ++V  LFR  A+L
Sbjct: 216 NRRVFISFSKICYLHQHLSK-SVKLFNEVFGVSLLVLFGNSFLSIVLALFRTAAEL 270


>AM292324-1|CAL23136.1|  398|Tribolium castaneum gustatory receptor
           candidate 3 protein.
          Length = 398

 Score = 28.3 bits (60), Expect = 0.15
 Identities = 16/56 (28%), Positives = 29/56 (51%), Gaps = 1/56 (1%)

Query: 126 NFRGLINLTELHLDHNHIEQMSSETFRHLTSLKTLILANNQISTLVSRLFRMLAKL 181
           N R  I+ +++   H H+ + S + F  +  +  L+L  N   ++V  LFR  A+L
Sbjct: 234 NRRVFISFSKICYLHQHLSK-SVKLFNEVFGVSLLVLFGNSFLSIVLALFRTAAEL 288


>AY008296-1|AAG22858.1|  556|Tribolium castaneum Dorsal protein.
          Length = 556

 Score = 24.6 bits (51), Expect = 1.8
 Identities = 10/31 (32%), Positives = 14/31 (45%)

Query: 528 KRREHDHEHPEELLGHTELGSVATIHAPYQL 558
           KR+   +  P +LL H E       H P+ L
Sbjct: 352 KRKRQKYHDPSQLLRHVEESDKQRTHQPFLL 382


>DQ855492-1|ABH88179.1|  251|Tribolium castaneum chemosensory
           protein 6 protein.
          Length = 251

 Score = 22.6 bits (46), Expect = 7.4
 Identities = 7/16 (43%), Positives = 10/16 (62%)

Query: 58  VIIAAPPDRPNFLTIG 73
           V++  PP  P F T+G
Sbjct: 190 VVVTKPPQAPPFATVG 205


>AY884065-1|AAX84206.1|  697|Tribolium castaneum laccase 1
          protein.
          Length = 697

 Score = 22.2 bits (45), Expect = 9.8
 Identities = 10/18 (55%), Positives = 10/18 (55%)

Query: 21 TCTMTFDLEEGHTTQVAC 38
          TC  TF LE  HT   AC
Sbjct: 56 TCRYTFLLEWYHTLSKAC 73


  Database: tribolium
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 114,650
  Number of sequences in database:  317
  
Lambda     K      H
   0.322    0.136    0.403 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 119,029
Number of Sequences: 317
Number of extensions: 4822
Number of successful extensions: 51
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 7
Number of HSP's gapped (non-prelim): 13
length of query: 563
length of database: 114,650
effective HSP length: 60
effective length of query: 503
effective length of database: 95,630
effective search space: 48101890
effective search space used: 48101890
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 45 (22.2 bits)