Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000680-TA|BGIBMGA000680-PA|IPR008147|Glutamine
synthetase, beta-Grasp, IPR008146|Glutamine synthetase, catalytic
region
         (422 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23H4.06 |gln1||glutamate-ammonia ligase Gln1|Schizosaccharom...   349   4e-97
SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces pom...    29   1.2  
SPAC17A5.12 |ucp7||UBA/TPR/DNAJ domain protein Ucp7|Schizosaccha...    27   5.0  

>SPAC23H4.06 |gln1||glutamate-ammonia ligase
           Gln1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 359

 Score =  349 bits (858), Expect = 4e-97
 Identities = 165/308 (53%), Positives = 211/308 (68%), Gaps = 5/308 (1%)

Query: 111 PKDQEDLPIWNFDGSSTNQADGHNSDTYLVPRAIYKDPFRRGNHILVMCDTYKYNMEPTE 170
           P   + L +WNFDGSST QA G+NSDT L P A+Y DPFRRG++ILV+   Y  +  P  
Sbjct: 52  PSSIDQLRVWNFDGSSTGQAPGNNSDTLLKPVAMYNDPFRRGDNILVLAACYTADGSPNG 111

Query: 171 SNNRISCQEAYDKCKDDEPWFGIEQEYILLDSDLRXXXXXXXXXXXXXXXYYCGVGANKV 230
            N+R +C +  +K  D E WFGIEQEY +LD   R               +YCGVG  +V
Sbjct: 112 FNHRDACAKLLEKHADKETWFGIEQEYTMLDYYDRPFGWPKGGFPGPQGPFYCGVGTGRV 171

Query: 231 FARDLVEAHYRCCLYAGVPIAGTNAEVMPSQWEFQVGPSIGVHAGDDLWVARYILHRLAE 290
           FARD+VEAHY+ CLYAG+ I+G NAEVMPSQWE+QVGP  G+  GD LW++R++LHR+AE
Sbjct: 172 FARDIVEAHYKACLYAGINISGINAEVMPSQWEYQVGPCAGIEMGDQLWMSRFLLHRIAE 231

Query: 291 EYGVIVTFDPKPV-QDWNGSGAHTNFSTKKMRNDNGIIEIEKAIDKLSKVHMKHIKVYDP 349
           ++GV ++F PKP+  DWNG+G HTN STK  R + GI  IE  ++K +K H +HI VY  
Sbjct: 232 DFGVKISFHPKPILGDWNGAGCHTNVSTKDTRAEGGIKAIESYLEKFAKRHKEHIAVY-- 289

Query: 350 RGGKDNERRLTGLHETASINDFSAGVANRGSSIRIPRSVAEDKKGYLEDRRPASNCDPYS 409
             G DN+ RLTG HET SI+ F+ GVA+RG+S+RIPRSVA +  GY EDRRPAS+ DPY 
Sbjct: 290 --GDDNDLRLTGRHETGSIDKFTYGVADRGASVRIPRSVAMNGCGYFEDRRPASSIDPYL 347

Query: 410 VIDALMRT 417
           V   +  T
Sbjct: 348 VTGIITET 355



 Score = 39.9 bits (89), Expect = 7e-04
 Identities = 23/48 (47%), Positives = 31/48 (64%), Gaps = 3/48 (6%)

Query: 28 VLSKT-LLSRYNDLPLPADKILATYIWIDGSGEHLRCKDRTLNFIPKA 74
          +LSK  +L++Y DLP    K++A YIWIDG   HLR K  TL+  P +
Sbjct: 9  LLSKAAILNKYADLPQNG-KVMAEYIWIDGF-NHLRSKTMTLDAKPSS 54


>SPBC405.02c ||SPBC4C3.01|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 447

 Score = 29.1 bits (62), Expect = 1.2
 Identities = 18/76 (23%), Positives = 35/76 (46%), Gaps = 3/76 (3%)

Query: 9   IEDNPKILSGPVLTNSPNAVLSKTLLSRYNDLPLPADKILATYIWIDGSGEHLRCKDRTL 68
           ++DN  + S     +S N+ LS +    +  +P+PA  +  T +  D +    R    T 
Sbjct: 295 LQDNASLTSQGSNLSSQNSGLSSSSSGIFGRMPIPAQSLDTTMLRTDSNSNIRRA---TT 351

Query: 69  NFIPKAPKDKLSGILE 84
           +FI  + K+  S  ++
Sbjct: 352 SFIANSEKENSSNFID 367


>SPAC17A5.12 |ucp7||UBA/TPR/DNAJ domain protein
           Ucp7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 697

 Score = 27.1 bits (57), Expect = 5.0
 Identities = 13/39 (33%), Positives = 20/39 (51%)

Query: 6   HTKIEDNPKILSGPVLTNSPNAVLSKTLLSRYNDLPLPA 44
           H KIE+N + +S P ++ S    L    L  +  + LPA
Sbjct: 116 HDKIEENKRPISQPQVSASEKVTLKDLSLEPHQPVSLPA 154


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.136    0.421 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,097,398
Number of Sequences: 5004
Number of extensions: 93898
Number of successful extensions: 198
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 192
Number of HSP's gapped (non-prelim): 4
length of query: 422
length of database: 2,362,478
effective HSP length: 75
effective length of query: 347
effective length of database: 1,987,178
effective search space: 689550766
effective search space used: 689550766
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 55 (26.2 bits)