Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000675-TA|BGIBMGA000675-PA|IPR004837|Sodium/calcium
exchanger membrane region, IPR004481|K+-dependent Na+/Ca+ exchanger
related-protein
(422 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_43713| Best HMM Match : Na_Ca_ex (HMM E-Value=7.9e-32) 144 1e-34
SB_42413| Best HMM Match : No HMM Matches (HMM E-Value=.) 133 2e-31
SB_45810| Best HMM Match : Na_Ca_ex (HMM E-Value=0) 128 1e-29
SB_15681| Best HMM Match : Na_Ca_ex (HMM E-Value=1.9e-36) 101 8e-22
SB_28247| Best HMM Match : Na_Ca_ex (HMM E-Value=2e-27) 99 8e-21
SB_42414| Best HMM Match : No HMM Matches (HMM E-Value=.) 90 3e-18
SB_8443| Best HMM Match : Calx-beta (HMM E-Value=0) 34 0.25
SB_28146| Best HMM Match : Na_Ca_ex (HMM E-Value=0.039) 33 0.44
SB_15333| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 1.3
SB_44420| Best HMM Match : Calx-beta (HMM E-Value=2e-22) 31 1.8
SB_35109| Best HMM Match : DUF1174 (HMM E-Value=6) 31 2.3
SB_11236| Best HMM Match : 7tm_1 (HMM E-Value=0) 30 3.1
SB_10745| Best HMM Match : 7tm_1 (HMM E-Value=2.2e-31) 30 3.1
SB_50801| Best HMM Match : 7tm_1 (HMM E-Value=1.4e-32) 30 4.1
SB_54687| Best HMM Match : Trp_Tyr_perm (HMM E-Value=2.2) 29 9.4
>SB_43713| Best HMM Match : Na_Ca_ex (HMM E-Value=7.9e-32)
Length = 228
Score = 144 bits (349), Expect = 1e-34
Identities = 64/157 (40%), Positives = 99/157 (63%), Gaps = 1/157 (0%)
Query: 58 EQLRQGAFLLYVVFGIYTFTLLAIVCNDYFIPCVELICEDLKIPQNVAAATFMSVATSCP 117
++ +QG + +V IYTF LA VC++YF+ +E IC+ L + +VA ATFM+ +S P
Sbjct: 5 QRQKQGGIVFHVFVAIYTFAALATVCDEYFVSSLERICDGLNLKSDVAGATFMAAGSSAP 64
Query: 118 EFFVNVISTFLTESDMGIGTIVGSALFNXXXXXXXXXXXXXTPIAIDMRPVTRDVLIYMV 177
EFF +VI F+T+ D+GIGTIVGSA+FN + + + P+TRD YM+
Sbjct: 65 EFFTSVIGVFITKGDIGIGTIVGSAVFNILFIVAICGMFAGSVLTLSWWPLTRDSFCYML 124
Query: 178 NISVLVAFVWDGQIDWFEAMVLGILYILYFVVM-FNS 213
+++ LV +D ++ W+E M L ++Y+LY +M FNS
Sbjct: 125 SVAALVIITYDKEVHWYEGMALVLMYLLYVTIMYFNS 161
Score = 28.7 bits (61), Expect = 9.4
Identities = 16/53 (30%), Positives = 25/53 (47%)
Query: 282 IPESVMGMTFLAFGGCLPEACSIFIMSRRGEGGIGVSNALGANSLAILFALGL 334
+ V G TF+A G PE + I +G IG+ +G+ ILF + +
Sbjct: 47 LKSDVAGATFMAAGSSAPEFFTSVIGVFITKGDIGIGTIVGSAVFNILFIVAI 99
>SB_42413| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 639
Score = 133 bits (322), Expect = 2e-31
Identities = 60/181 (33%), Positives = 104/181 (57%)
Query: 54 IFTDEQLRQGAFLLYVVFGIYTFTLLAIVCNDYFIPCVELICEDLKIPQNVAAATFMSVA 113
IFT ++ R GA +++ +Y F LAI+C++YF +E +C+ L+IP +VA ATFM+
Sbjct: 39 IFTLQERRHGAVIIHTFICLYCFVALAILCDNYFCASLEKLCKRLRIPTDVAGATFMAAG 98
Query: 114 TSCPEFFVNVISTFLTESDMGIGTIVGSALFNXXXXXXXXXXXXXTPIAIDMRPVTRDVL 173
+S P F+ + S F+ E D+G+GTI+GS++FN I++ P+ RD
Sbjct: 99 SSMPTLFIAIASVFMGEGDIGLGTIIGSSMFNILFITAICGLFSGMVISLHTWPIVRDSC 158
Query: 174 IYMVNISVLVAFVWDGQIDWFEAMVLGILYILYFVVMFNSMKAFVLIDKIWAACCKKNNG 233
+Y+VN+ L+ + D I ++EA++ +LY Y ++M + + + + CC N G
Sbjct: 159 VYVVNLVGLLIVIHDNVIHFYEALIFPVLYTGYILIMVFNTRLENAFESAFETCCDINKG 218
Query: 234 V 234
+
Sbjct: 219 M 219
Score = 81.8 bits (193), Expect = 1e-15
Identities = 43/132 (32%), Positives = 72/132 (54%), Gaps = 5/132 (3%)
Query: 277 GHTFFIPESVMGMTFLAFGGCLPEACSIFIMSRRGEGGIGVSNALGANSLAILFALGLPW 336
G TF IPE +MG+T LA G +P+ S I+++ G G + ++N +G+N +L LGLPW
Sbjct: 475 GETFSIPEYIMGLTLLAAGSSVPDVMSSLIVAKHGMGDMALANCIGSNIFDVL-CLGLPW 533
Query: 337 LIRTVSLEIQGAENTAVLINSAGIDFXXXXXXXXXXXXWIVLFIGKFTLRRTVGIVLGIL 396
L+ T ++ N+ VLI+S I + + + + L R +G++L I
Sbjct: 534 LLATTAVH----PNSVVLIHSGHIVYVSMCLFGTVLTIVSAIHLNGWRLDRRLGVILFIA 589
Query: 397 YVVFIVFAILVE 408
Y F+ A+++E
Sbjct: 590 YAFFLTSAVILE 601
Score = 35.9 bits (79), Expect = 0.062
Identities = 18/49 (36%), Positives = 24/49 (48%)
Query: 282 IPESVMGMTFLAFGGCLPEACSIFIMSRRGEGGIGVSNALGANSLAILF 330
IP V G TF+A G +P GEG IG+ +G++ ILF
Sbjct: 85 IPTDVAGATFMAAGSSMPTLFIAIASVFMGEGDIGLGTIIGSSMFNILF 133
Score = 31.1 bits (67), Expect = 1.8
Identities = 14/55 (25%), Positives = 27/55 (49%)
Query: 91 VELICEDLKIPQNVAAATFMSVATSCPEFFVNVISTFLTESDMGIGTIVGSALFN 145
V +I E IP+ + T ++ +S P+ ++I DM + +GS +F+
Sbjct: 471 VSIIGETFSIPEYIMGLTLLAAGSSVPDVMSSLIVAKHGMGDMALANCIGSNIFD 525
>SB_45810| Best HMM Match : Na_Ca_ex (HMM E-Value=0)
Length = 582
Score = 128 bits (308), Expect = 1e-29
Identities = 65/165 (39%), Positives = 98/165 (59%), Gaps = 1/165 (0%)
Query: 59 QLRQGAFLLYVVFGIYTFTLLAIVCNDYFIPCVELICEDLKIPQNVAAATFMSVATSCPE 118
QL G + L V F + LAIVC+D+F+P +E I E L + ++VA ATFM+ +S PE
Sbjct: 11 QLGFGDYFLIVDFFRFFRLALAIVCDDFFVPSLEAISEKLDLSEDVAGATFMAAGSSAPE 70
Query: 119 FFVNVISTFLTESDMGIGTIVGSALFNXXXXXXXXXXXXXTPIAIDMRPVTRDVLIYMVN 178
F ++ + +SD+G+GTIVGSA+FN + +D RP+ RD + Y ++
Sbjct: 71 LFTSIAGVTV-DSDVGVGTIVGSAIFNLLVIIALTAAFSGQVLQLDWRPLLRDSVAYSLS 129
Query: 179 ISVLVAFVWDGQIDWFEAMVLGILYILYFVVMFNSMKAFVLIDKI 223
I+ + F WDG+ + +EA VL ILYI Y VVM + K L+ ++
Sbjct: 130 IAFFIIFSWDGKFEMYEAAVLLILYIAYIVVMKFNPKLMDLLAEV 174
Score = 58.0 bits (134), Expect = 1e-08
Identities = 39/145 (26%), Positives = 74/145 (51%), Gaps = 11/145 (7%)
Query: 275 KNGHTFFIPESVMGMTFLAFGGCLPEACSIFIMSRRGEGGIGVSNALGANSLAILFALGL 334
++G + + MG+ +A G +P+A S I++R G G + VSNA+G+N I LGL
Sbjct: 433 RSGCILNVDKFTMGLVIIAIGTSVPDALSSIIVARDGFGDMAVSNAIGSNVFDINLGLGL 492
Query: 335 PWLIRTV--SLE----IQGAE----NTAVLINSAGIDFXXXXXXXXXXXXWIVLFIGKFT 384
P++IR + +E + AE T ++ S + F +++ KF
Sbjct: 493 PFVIRILIDKMEPIRMLTPAEEIMLETGEIVISPHVKFGFLLLLFLVIALFLMAAF-KFK 551
Query: 385 LRRTVGIVLGILYVVFIVFAILVEL 409
L + +G+ +YV+F+++A + ++
Sbjct: 552 LNKRLGLSFVFMYVLFVLYAYIQDI 576
>SB_15681| Best HMM Match : Na_Ca_ex (HMM E-Value=1.9e-36)
Length = 183
Score = 101 bits (243), Expect = 8e-22
Identities = 51/139 (36%), Positives = 81/139 (58%), Gaps = 2/139 (1%)
Query: 74 YTFTLLAIVCNDYFIPCVELICEDLKIPQNVAAATFMSVATSCPEFFVNVISTFL-TESD 132
Y F LAI C+++F+P + +I E L++ ++VA ATFM+ S PE F + I TF+ +S+
Sbjct: 3 YMFLALAIACDEFFVPSLTVIIEKLELKEDVAGATFMAAGGSAPELFTSFIGTFIDPKSN 62
Query: 133 MGIGTIVGSALFNXXXXXXXXXXXXXTPIAIDMRPVTRDVLIYMVNISVLVAFVWDGQ-I 191
+GIGTIVGSA+FN + + P+ RD + Y + + +L+ F G I
Sbjct: 63 VGIGTIVGSAVFNVLFVIGMCAMFSKGVLELTWWPLFRDCIFYSLALIILIIFFLSGDVI 122
Query: 192 DWFEAMVLGILYILYFVVM 210
W+E++VL + Y Y + M
Sbjct: 123 VWWESLVLLLCYFAYVIFM 141
Score = 33.9 bits (74), Expect = 0.25
Identities = 17/54 (31%), Positives = 29/54 (53%), Gaps = 1/54 (1%)
Query: 282 IPESVMGMTFLAFGGCLPEACSIFIMS-RRGEGGIGVSNALGANSLAILFALGL 334
+ E V G TF+A GG PE + FI + + +G+ +G+ +LF +G+
Sbjct: 29 LKEDVAGATFMAAGGSAPELFTSFIGTFIDPKSNVGIGTIVGSAVFNVLFVIGM 82
>SB_28247| Best HMM Match : Na_Ca_ex (HMM E-Value=2e-27)
Length = 236
Score = 98.7 bits (235), Expect = 8e-21
Identities = 46/133 (34%), Positives = 81/133 (60%), Gaps = 4/133 (3%)
Query: 277 GHTFFIPESVMGMTFLAFGGCLPEACSIFIMSRRGEGGIGVSNALGANSLAILFALGLPW 336
G+TF IP+SVMG++ +AFG +P+ S ++R+G+G + VS+ +G+N IL LG+PW
Sbjct: 93 GYTFLIPDSVMGLSLVAFGSSVPDCLSSLFVARKGDGDMAVSHTVGSNVFDILLCLGIPW 152
Query: 337 LIRTVSLEIQGAENTAVLINSAGIDFXXXXXXXXXXXXWIVLFIGKFTLRRTVGIVLGIL 396
LI+T E +++V+INS G+ I+++ K+TL + VG + +
Sbjct: 153 LIKTTVWEY----DSSVVINSHGLFISCFFILGSIAVTLIIIWYYKWTLNKKVGCIYLVF 208
Query: 397 YVVFIVFAILVEL 409
Y +F+ +++VE+
Sbjct: 209 YFIFMSISVVVEM 221
>SB_42414| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 360
Score = 90.2 bits (214), Expect = 3e-18
Identities = 47/132 (35%), Positives = 75/132 (56%), Gaps = 5/132 (3%)
Query: 277 GHTFFIPESVMGMTFLAFGGCLPEACSIFIMSRRGEGGIGVSNALGANSLAILFALGLPW 336
G+T+ IPE VMGMTFLA G LP+A + +++++G G + VSN +G+N +L LG+PW
Sbjct: 223 GYTYTIPECVMGMTFLAAGSSLPDAIASLVVAKQGSGDMAVSNCIGSNVFDML-CLGIPW 281
Query: 337 LIRTVSLEIQGAENTAVLINSAGIDFXXXXXXXXXXXXWIVLFIGKFTLRRTVGIVLGIL 396
LI++ L+ V+I S I F ++++ K+ L VGI I+
Sbjct: 282 LIKSAFLQ----PGKPVVIQSENIFFTSAMLIGSIAVTFLLIQFNKWKLNVKVGIAFLIM 337
Query: 397 YVVFIVFAILVE 408
Y +F++ A +E
Sbjct: 338 YFLFLIVATYIE 349
Score = 42.7 bits (96), Expect = 5e-04
Identities = 13/42 (30%), Positives = 29/42 (69%)
Query: 170 RDVLIYMVNISVLVAFVWDGQIDWFEAMVLGILYILYFVVMF 211
RD +Y+ ++ VL+ ++D ++ W+E ++ I+Y +Y V+M+
Sbjct: 60 RDSTVYVASVIVLMVSIYDNKVYWYEGLIFVIVYAIYIVIMY 101
Score = 35.1 bits (77), Expect = 0.11
Identities = 20/78 (25%), Positives = 38/78 (48%), Gaps = 3/78 (3%)
Query: 71 FGIYTFTLLAI---VCNDYFIPCVELICEDLKIPQNVAAATFMSVATSCPEFFVNVISTF 127
F + TFT+ I V + + + V +I IP+ V TF++ +S P+ +++
Sbjct: 196 FYLVTFTVAVIWMAVLSYFLVWMVAIIGYTYTIPECVMGMTFLAAGSSLPDAIASLVVAK 255
Query: 128 LTESDMGIGTIVGSALFN 145
DM + +GS +F+
Sbjct: 256 QGSGDMAVSNCIGSNVFD 273
>SB_8443| Best HMM Match : Calx-beta (HMM E-Value=0)
Length = 694
Score = 33.9 bits (74), Expect = 0.25
Identities = 18/47 (38%), Positives = 26/47 (55%), Gaps = 3/47 (6%)
Query: 102 QNVAAATFMSVATSCPEFFVNVISTF---LTESDMGIGTIVGSALFN 145
+ V+ T M++ +S PE ++VI D+G TIVGSA FN
Sbjct: 64 ETVSNLTLMALGSSAPEILLSVIEIIGNNFKAGDLGPSTIVGSAAFN 110
>SB_28146| Best HMM Match : Na_Ca_ex (HMM E-Value=0.039)
Length = 751
Score = 33.1 bits (72), Expect = 0.44
Identities = 17/48 (35%), Positives = 27/48 (56%)
Query: 163 IDMRPVTRDVLIYMVNISVLVAFVWDGQIDWFEAMVLGILYILYFVVM 210
++ RP TRDVL Y+ +S + ++ +I EA+ I Y Y VV+
Sbjct: 589 LNNRPFTRDVLFYLGAVSWMFITMYRQRITMLEAIGFIIFYFAYVVVV 636
>SB_15333| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 752
Score = 31.5 bits (68), Expect = 1.3
Identities = 34/106 (32%), Positives = 51/106 (48%), Gaps = 28/106 (26%)
Query: 67 LYVVFGIYTFTLLAIVCNDYFIPCVELIC--------------EDLKIP-----QNVAAA 107
LY++ +Y F +AIV D F+ C+E+I E ++I + VA
Sbjct: 40 LYILGMLYLFLGIAIVA-DIFMSCIEVITSKKRKVTRYDHEKGESVEIEVFVWNETVANL 98
Query: 108 TFMSVATSCPEFFVNVISTF-------LTESD-MGIGTIVGSALFN 145
T M++ +S PE + V+ T T +D +G TIVGSA FN
Sbjct: 99 TLMALGSSAPEILLAVVETGQELALGQTTATDGLGTFTIVGSASFN 144
>SB_44420| Best HMM Match : Calx-beta (HMM E-Value=2e-22)
Length = 461
Score = 31.1 bits (67), Expect = 1.8
Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 4/48 (8%)
Query: 102 QNVAAATFMSVATSCPEFFVNVISTFLTES----DMGIGTIVGSALFN 145
+ VA T M++ +S PE +++I + +G TIVGSA FN
Sbjct: 96 ETVANLTLMALGSSAPEILLSIIEITIMNGFEAGALGPSTIVGSAAFN 143
>SB_35109| Best HMM Match : DUF1174 (HMM E-Value=6)
Length = 369
Score = 30.7 bits (66), Expect = 2.3
Identities = 16/63 (25%), Positives = 29/63 (46%)
Query: 234 VTASLNDNNVSVEEGIDNKGFNDPSPVTNGTTMTEKPAIDEKNGHTFFIPESVMGMTFLA 293
V ++L +V++E+ +++ SP+ T M P G TF + + G TF+
Sbjct: 13 VKSTLAYEHVAIEDELESNANKSVSPIITDTFMITGPKRKGYGGITFIVTQGYGGTTFIV 72
Query: 294 FGG 296
G
Sbjct: 73 TQG 75
>SB_11236| Best HMM Match : 7tm_1 (HMM E-Value=0)
Length = 365
Score = 30.3 bits (65), Expect = 3.1
Identities = 14/42 (33%), Positives = 27/42 (64%), Gaps = 1/42 (2%)
Query: 382 KFTLRRTVGIVLGILYVVFIVFAILVELG-IIFDRNVELSSC 422
+F R+ I++G+L+ ++FAIL E+G +++ NV + C
Sbjct: 139 RFMTRKKACIIIGLLWTYSLLFAILPEIGWRLYENNVIDNVC 180
>SB_10745| Best HMM Match : 7tm_1 (HMM E-Value=2.2e-31)
Length = 354
Score = 30.3 bits (65), Expect = 3.1
Identities = 14/43 (32%), Positives = 24/43 (55%), Gaps = 1/43 (2%)
Query: 171 DVLIYMVNISVLVAFVWDGQIDWFEAMVLGILYILYFVVMFNS 213
DV++ ++ + VA+ WDG + WF +V I + F V + S
Sbjct: 61 DVIVTLIGCPITVAWFWDGML-WFGGLVGEITCRICFSVFYLS 102
>SB_50801| Best HMM Match : 7tm_1 (HMM E-Value=1.4e-32)
Length = 354
Score = 29.9 bits (64), Expect = 4.1
Identities = 16/63 (25%), Positives = 33/63 (52%), Gaps = 11/63 (17%)
Query: 171 DVLIYMVNISVLVAFVWDGQIDWFEAMV--------LGILYILYFVVMFNSMKAFVLIDK 222
DV + ++ + VA+ WDG + WF +V + + Y+ +V +F+ + F+ +D+
Sbjct: 61 DVTVTLIGCPITVAWFWDGML-WFGGLVGEITCRICISVFYLSCYVSLFSLL--FISVDR 117
Query: 223 IWA 225
A
Sbjct: 118 FLA 120
>SB_54687| Best HMM Match : Trp_Tyr_perm (HMM E-Value=2.2)
Length = 369
Score = 28.7 bits (61), Expect = 9.4
Identities = 10/25 (40%), Positives = 18/25 (72%)
Query: 71 FGIYTFTLLAIVCNDYFIPCVELIC 95
FG++ + ++AIVC Y++ C + IC
Sbjct: 324 FGLFFYGIIAIVCVVYWLLCGKCIC 348
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.326 0.142 0.437
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,189,706
Number of Sequences: 59808
Number of extensions: 488985
Number of successful extensions: 1486
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1455
Number of HSP's gapped (non-prelim): 28
length of query: 422
length of database: 16,821,457
effective HSP length: 84
effective length of query: 338
effective length of database: 11,797,585
effective search space: 3987583730
effective search space used: 3987583730
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.7 bits)
S2: 61 (28.7 bits)
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