Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000659-TA|BGIBMGA000659-PA|IPR013120|Male sterility
C-terminal, IPR004262|Male sterility
         (432 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi...    40   9e-04
SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb...    29   0.96 
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi...    29   1.3  
SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po...    29   1.3  
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S...    29   1.3  
SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces ...    29   1.7  
SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces pom...    29   1.7  
SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|...    29   1.7  
SPAC13F5.06c |sec10||exocyst complex subunit Sec10|Schizosacchar...    28   2.9  
SPAC12D12.09 |rev7||DNA polymerase zeta Rev7 |Schizosaccharomyce...    27   3.9  
SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces p...    27   3.9  
SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch...    27   3.9  
SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ...    27   5.1  
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch...    27   6.7  
SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ...    27   6.7  
SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31 |...    26   8.9  

>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1419

 Score = 39.5 bits (88), Expect = 9e-04
 Identities = 33/153 (21%), Positives = 64/153 (41%), Gaps = 8/153 (5%)

Query: 14   RNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLED-LQRLPLFDRLK 72
            + + +TG  G++G  ++  L+     +  V  L+R    +   +RL+D      ++D   
Sbjct: 1005 KTVFLTGANGYLGVFILRDLMTRSSNL-KVIALVRASSEEHGLKRLKDSCTAYGVWDE-- 1061

Query: 73   NERPNALNKIKAIQGDVLFEDFGLSNLEIENLSKEVTVVFHFAATLRLEAPLKDNVNMNT 132
                +   KI  + GD+  E++G+   +   L++ V  V H  A +    P       N 
Sbjct: 1062 ----SWAQKISVVNGDLALENWGIEERKWNKLTEVVDYVIHNGALVHWVYPYSKLRGPNV 1117

Query: 133  SGTLRTIKVAKKLKNLQIFVHLSTAFCYPDYEV 165
             GT+  +K+    K   +    ST+    +Y V
Sbjct: 1118 MGTITALKLCSLGKGKSLSFVSSTSTVDTEYYV 1150


>SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 258

 Score = 29.5 bits (63), Expect = 0.96
 Identities = 27/97 (27%), Positives = 46/97 (47%), Gaps = 8/97 (8%)

Query: 13  GRNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLE-DLQRLPLFDRL 71
           GR +L+TG  G +GKVL +        +  + I + P K +  A  L+ D+ +    D++
Sbjct: 5   GRVVLITGAAGGIGKVLCKMFTELGDRVAGIDI-VDPSKVQDAALALQADVSKA---DQI 60

Query: 72  KNERPNALNKIKAIQGDVLFEDFGLS-NLEIENLSKE 107
           +      +  +  I  DVL  + GL+ +   E LS E
Sbjct: 61  ETAIEKVIQTLGPI--DVLINNAGLADDTPFEQLSHE 95


>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
           Gap1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 766

 Score = 29.1 bits (62), Expect = 1.3
 Identities = 32/134 (23%), Positives = 68/134 (50%), Gaps = 13/134 (9%)

Query: 27  KVLVEKLLYSVPEIGNVYILMRP--KKAKTVAQRLEDLQRLPLFDRLKNERPNALNKIKA 84
           ++++E L        +V+++++   +  K+V Q    L  LPL++R  +  P+    +K 
Sbjct: 447 EIILENLDNLYDPDSHVHLILQELGEPCKSVPQEDNCLVTLPLYNRWDSSIPDLKQNLKV 506

Query: 85  IQGDVLFEDFGLSNLEIENLSKEVTVVFHFAATLRLEAPL-KDNVNMNTSGTLRTIKVAK 143
            + D+L+ D     L I+ L   +    H A  + L+ PL  D+V+     +L+++ + K
Sbjct: 507 TREDILYVD--AKTLFIQLL--RLLPSGHPATRVPLDLPLIADSVS-----SLKSMSLMK 557

Query: 144 K-LKNLQIFVHLST 156
           K ++ +++   LST
Sbjct: 558 KGIRAIELLDELST 571


>SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 981

 Score = 29.1 bits (62), Expect = 1.3
 Identities = 31/130 (23%), Positives = 59/130 (45%), Gaps = 12/130 (9%)

Query: 13  GRNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLEDLQRLPLFDRLK 72
           G+  L+TG TG+ G+  +E   Y V    +V  L+R    +   +RL     + L   L+
Sbjct: 644 GQYFLLTGATGYFGRRFLE---YLVKLNISVVCLVRESSDEAAKERL-----ISLVPSLR 695

Query: 73  NERPNALNKIKAIQGDVLFEDFGLSNLEIENLSKEVTVVFHFAATLRLEAPLKDNVNMNT 132
               N +  + A   + +   FGL + + E L + V+ ++H AA +      ++    N 
Sbjct: 696 ISSENII--VWAAHVEEI--RFGLDDAKWEFLVENVSRIYHMAAEVHWMKSYQELRPANV 751

Query: 133 SGTLRTIKVA 142
            GT   ++++
Sbjct: 752 LGTKTVLELS 761


>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 971

 Score = 29.1 bits (62), Expect = 1.3
 Identities = 21/79 (26%), Positives = 35/79 (44%), Gaps = 4/79 (5%)

Query: 334 YPLSWPLWYPNGDITTNYTLHEI--RRIFYHLVPAYCIDFLLFLMGQKRFLFVVHWVCKI 391
           YP+  P  YP+G ++   ++  +      Y       I F+L   G   F+++ H  C  
Sbjct: 501 YPIKGPQPYPHGAVSIVVSICAVLVAPYLYQSGAFMLIGFVLACFGSY-FMYINHGWCSY 559

Query: 392 LFWLLILSFVAAY-FEPVR 409
           L  L+  S+V  Y F  +R
Sbjct: 560 LGGLIFTSYVLIYSFASIR 578


>SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 227

 Score = 28.7 bits (61), Expect = 1.7
 Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 3/45 (6%)

Query: 188 DDRQLTLLTPSLLGPHPNCYTF-SKRLAENVVEQAYKDLPIVIAR 231
           D+   T+ TPS     P+ Y+  S R   NV+ QAYK L I + R
Sbjct: 169 DNTLPTITTPS--SSSPDAYSSGSSRRTSNVLRQAYKKLGIFVER 211


>SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 397

 Score = 28.7 bits (61), Expect = 1.7
 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%)

Query: 13  GRNILVTGGTGFMGKVLVEKLLYSVPEIG-NVYILMRPKKAKTVAQRLEDLQR 64
           G  ++VTGG+  +G+V+VEKL      +G  V IL+R +  +     + DL++
Sbjct: 75  GMVVMVTGGSSGIGQVVVEKL----ASLGAQVVILLRTEPDQFTVDYIMDLRK 123


>SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 336

 Score = 28.7 bits (61), Expect = 1.7
 Identities = 38/158 (24%), Positives = 76/158 (48%), Gaps = 28/158 (17%)

Query: 12  AGRNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLEDLQRLPLFDRL 71
           +G+ +LVTG TGF+G  + E+LL    + G      R +      ++ ++L RL      
Sbjct: 2   SGKLVLVTGVTGFIGAHVAEQLL----QAG-----YRVRGTVRSMEKADELIRL------ 46

Query: 72  KNERPNALNKIKAIQGDVLFEDFGLSNLEIENLSKEVTVVFHFAATLRLEAPLKDN---- 127
               P   +KI+     V+ +D   SN   + + K+V ++ H A+   +E  + DN    
Sbjct: 47  ---NPGLKDKIEF----VIVKDVSASN-AFDGVLKDVELICHIASPFFVE-NVTDNKSQL 97

Query: 128 VNMNTSGTLRTIKVAKKLKNLQIFVHLSTAFCYPDYEV 165
           ++    GTL  ++ A+ +K+++  V  S+     ++++
Sbjct: 98  LDPAVKGTLGILEAAQGVKSIKRIVITSSFAAVGNFQI 135


>SPAC13F5.06c |sec10||exocyst complex subunit
           Sec10|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 811

 Score = 27.9 bits (59), Expect = 2.9
 Identities = 20/71 (28%), Positives = 37/71 (52%), Gaps = 3/71 (4%)

Query: 79  LNKIKAIQGDVLFEDFGLSNLEIENL--SKEVTVVFHFAATLRLEAPLKDNVNMNTSGTL 136
           LN++K+  GD +  D+  ++ +++N    K   +   FA  L L   L+DNVN   SG +
Sbjct: 65  LNRLKSRVGDRI-RDYASASKQVQNEYHQKSNHLREKFAQVLELSRHLEDNVNDMRSGLV 123

Query: 137 RTIKVAKKLKN 147
              +  ++ +N
Sbjct: 124 DAGQELERAEN 134


>SPAC12D12.09 |rev7||DNA polymerase zeta Rev7 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 213

 Score = 27.5 bits (58), Expect = 3.9
 Identities = 12/31 (38%), Positives = 18/31 (58%)

Query: 295 EGTKKEKSLTIPVYNVNIGHQKPMTWGDVLT 325
           + T++ K +TIPV NV+IG      W +  T
Sbjct: 180 DATRQFKGITIPVRNVDIGPMMTEIWVEKYT 210


>SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 409

 Score = 27.5 bits (58), Expect = 3.9
 Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 2/64 (3%)

Query: 67  LFDRLKNERPNALNKIKAIQGDVLFEDFGLS-NLEIENLSKEVTVVFHFAATLRLEAPLK 125
           + DR   E P AL  ++ ++G +L +  G++    I    KE + +F     + L  P K
Sbjct: 127 VLDRTSKEEPWALRLLRQLEGIMLLQKNGITREFPIWGYYKE-SSIFGIIDEISLNNPSK 185

Query: 126 DNVN 129
            N N
Sbjct: 186 SNFN 189


>SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1316

 Score = 27.5 bits (58), Expect = 3.9
 Identities = 25/69 (36%), Positives = 35/69 (50%), Gaps = 12/69 (17%)

Query: 32  KLLYSVPEI-GNVYILMRPKKAKTVAQRLEDLQRLPLFDRLKNERPNALNKIKAIQGDVL 90
           K LY++PE    +YI         VA  LE L RL +  R  + +P+  N + +I G + 
Sbjct: 655 KSLYTIPESWAKIYIA-------EVALGLEHLHRLGIIHR--DIKPD--NILMSITGHLK 703

Query: 91  FEDFGLSNL 99
             DFGLS L
Sbjct: 704 LADFGLSQL 712


>SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr
          1|||Manual
          Length = 255

 Score = 27.1 bits (57), Expect = 5.1
 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 2/49 (4%)

Query: 13 GRNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLED 61
          G+  L+TGG+G +G  + +    +   +G +Y   R KKA   A  L D
Sbjct: 9  GKTTLITGGSGGIGFSIAKAFAAAGSNVGLLY--GRNKKALEYAAELRD 55


>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2176

 Score = 26.6 bits (56), Expect = 6.7
 Identities = 23/77 (29%), Positives = 35/77 (45%), Gaps = 4/77 (5%)

Query: 178  EHVMRLIEWLDDRQLTLLTPSLLGPHPNCYTFSKRLAENVVEQAYKDLPIVIARPSIVCP 237
            E    L ++ DD  +   T  LL P P CY F K +++  +    K +P+   R  I+  
Sbjct: 1275 EQFFLLKKYKDDEHIVNFTVPLLEPLPPCY-FIKIVSDRWLHSITK-VPLSFQR-LIMPE 1331

Query: 238  SYSEPMPGWVDNLNGPV 254
             +  P P  +D  N PV
Sbjct: 1332 KFPAPTP-LLDLQNAPV 1347


>SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 937

 Score = 26.6 bits (56), Expect = 6.7
 Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 2/57 (3%)

Query: 210 SKRLAENVVEQAYKDLPIVIARPSIVCPSYSEPMPGWVDNLNGPVGVM-IGAGKGVI 265
           +K   E   +Q Y +LPI IA+      S+   + G   N   P+  M + AG G I
Sbjct: 848 AKERLETFTKQGYNNLPICIAKTQYSL-SHDPDLKGAPTNFTVPIRDMRLSAGAGFI 903


>SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1032

 Score = 26.2 bits (55), Expect = 8.9
 Identities = 14/41 (34%), Positives = 20/41 (48%)

Query: 67  LFDRLKNERPNALNKIKAIQGDVLFEDFGLSNLEIENLSKE 107
           +F+ L   RP   N +KAI   +   D G +    E+ SKE
Sbjct: 806 IFEMLTGTRPFEANTVKAIWARIERNDIGWTKRVKESCSKE 846


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.323    0.140    0.432 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,002,080
Number of Sequences: 5004
Number of extensions: 87238
Number of successful extensions: 221
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 214
Number of HSP's gapped (non-prelim): 17
length of query: 432
length of database: 2,362,478
effective HSP length: 75
effective length of query: 357
effective length of database: 1,987,178
effective search space: 709422546
effective search space used: 709422546
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 55 (26.2 bits)