BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000659-TA|BGIBMGA000659-PA|IPR013120|Male sterility C-terminal, IPR004262|Male sterility (432 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 40 9e-04 SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 29 0.96 SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 29 1.3 SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces po... 29 1.3 SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 29 1.3 SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces ... 29 1.7 SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces pom... 29 1.7 SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|... 29 1.7 SPAC13F5.06c |sec10||exocyst complex subunit Sec10|Schizosacchar... 28 2.9 SPAC12D12.09 |rev7||DNA polymerase zeta Rev7 |Schizosaccharomyce... 27 3.9 SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces p... 27 3.9 SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 27 3.9 SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 27 5.1 SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 27 6.7 SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces ... 27 6.7 SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31 |... 26 8.9 >SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1419 Score = 39.5 bits (88), Expect = 9e-04 Identities = 33/153 (21%), Positives = 64/153 (41%), Gaps = 8/153 (5%) Query: 14 RNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLED-LQRLPLFDRLK 72 + + +TG G++G ++ L+ + V L+R + +RL+D ++D Sbjct: 1005 KTVFLTGANGYLGVFILRDLMTRSSNL-KVIALVRASSEEHGLKRLKDSCTAYGVWDE-- 1061 Query: 73 NERPNALNKIKAIQGDVLFEDFGLSNLEIENLSKEVTVVFHFAATLRLEAPLKDNVNMNT 132 + KI + GD+ E++G+ + L++ V V H A + P N Sbjct: 1062 ----SWAQKISVVNGDLALENWGIEERKWNKLTEVVDYVIHNGALVHWVYPYSKLRGPNV 1117 Query: 133 SGTLRTIKVAKKLKNLQIFVHLSTAFCYPDYEV 165 GT+ +K+ K + ST+ +Y V Sbjct: 1118 MGTITALKLCSLGKGKSLSFVSSTSTVDTEYYV 1150 >SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 29.5 bits (63), Expect = 0.96 Identities = 27/97 (27%), Positives = 46/97 (47%), Gaps = 8/97 (8%) Query: 13 GRNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLE-DLQRLPLFDRL 71 GR +L+TG G +GKVL + + + I + P K + A L+ D+ + D++ Sbjct: 5 GRVVLITGAAGGIGKVLCKMFTELGDRVAGIDI-VDPSKVQDAALALQADVSKA---DQI 60 Query: 72 KNERPNALNKIKAIQGDVLFEDFGLS-NLEIENLSKE 107 + + + I DVL + GL+ + E LS E Sbjct: 61 ETAIEKVIQTLGPI--DVLINNAGLADDTPFEQLSHE 95 >SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schizosaccharomyces pombe|chr 2|||Manual Length = 766 Score = 29.1 bits (62), Expect = 1.3 Identities = 32/134 (23%), Positives = 68/134 (50%), Gaps = 13/134 (9%) Query: 27 KVLVEKLLYSVPEIGNVYILMRP--KKAKTVAQRLEDLQRLPLFDRLKNERPNALNKIKA 84 ++++E L +V+++++ + K+V Q L LPL++R + P+ +K Sbjct: 447 EIILENLDNLYDPDSHVHLILQELGEPCKSVPQEDNCLVTLPLYNRWDSSIPDLKQNLKV 506 Query: 85 IQGDVLFEDFGLSNLEIENLSKEVTVVFHFAATLRLEAPL-KDNVNMNTSGTLRTIKVAK 143 + D+L+ D L I+ L + H A + L+ PL D+V+ +L+++ + K Sbjct: 507 TREDILYVD--AKTLFIQLL--RLLPSGHPATRVPLDLPLIADSVS-----SLKSMSLMK 557 Query: 144 K-LKNLQIFVHLST 156 K ++ +++ LST Sbjct: 558 KGIRAIELLDELST 571 >SPCC162.02c |||AMP-binding dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 981 Score = 29.1 bits (62), Expect = 1.3 Identities = 31/130 (23%), Positives = 59/130 (45%), Gaps = 12/130 (9%) Query: 13 GRNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLEDLQRLPLFDRLK 72 G+ L+TG TG+ G+ +E Y V +V L+R + +RL + L L+ Sbjct: 644 GQYFLLTGATGYFGRRFLE---YLVKLNISVVCLVRESSDEAAKERL-----ISLVPSLR 695 Query: 73 NERPNALNKIKAIQGDVLFEDFGLSNLEIENLSKEVTVVFHFAATLRLEAPLKDNVNMNT 132 N + + A + + FGL + + E L + V+ ++H AA + ++ N Sbjct: 696 ISSENII--VWAAHVEEI--RFGLDDAKWEFLVENVSRIYHMAAEVHWMKSYQELRPANV 751 Query: 133 SGTLRTIKVA 142 GT ++++ Sbjct: 752 LGTKTVLELS 761 >SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 971 Score = 29.1 bits (62), Expect = 1.3 Identities = 21/79 (26%), Positives = 35/79 (44%), Gaps = 4/79 (5%) Query: 334 YPLSWPLWYPNGDITTNYTLHEI--RRIFYHLVPAYCIDFLLFLMGQKRFLFVVHWVCKI 391 YP+ P YP+G ++ ++ + Y I F+L G F+++ H C Sbjct: 501 YPIKGPQPYPHGAVSIVVSICAVLVAPYLYQSGAFMLIGFVLACFGSY-FMYINHGWCSY 559 Query: 392 LFWLLILSFVAAY-FEPVR 409 L L+ S+V Y F +R Sbjct: 560 LGGLIFTSYVLIYSFASIR 578 >SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces pombe|chr 3|||Manual Length = 227 Score = 28.7 bits (61), Expect = 1.7 Identities = 18/45 (40%), Positives = 24/45 (53%), Gaps = 3/45 (6%) Query: 188 DDRQLTLLTPSLLGPHPNCYTF-SKRLAENVVEQAYKDLPIVIAR 231 D+ T+ TPS P+ Y+ S R NV+ QAYK L I + R Sbjct: 169 DNTLPTITTPS--SSSPDAYSSGSSRRTSNVLRQAYKKLGIFVER 211 >SPAC19A8.06 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 397 Score = 28.7 bits (61), Expect = 1.7 Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 5/53 (9%) Query: 13 GRNILVTGGTGFMGKVLVEKLLYSVPEIG-NVYILMRPKKAKTVAQRLEDLQR 64 G ++VTGG+ +G+V+VEKL +G V IL+R + + + DL++ Sbjct: 75 GMVVMVTGGSSGIGQVVVEKL----ASLGAQVVILLRTEPDQFTVDYIMDLRK 123 >SPAC513.07 |||flavonol reductase/cinnamoyl-CoA reductase family|Schizosaccharomyces pombe|chr 1|||Manual Length = 336 Score = 28.7 bits (61), Expect = 1.7 Identities = 38/158 (24%), Positives = 76/158 (48%), Gaps = 28/158 (17%) Query: 12 AGRNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLEDLQRLPLFDRL 71 +G+ +LVTG TGF+G + E+LL + G R + ++ ++L RL Sbjct: 2 SGKLVLVTGVTGFIGAHVAEQLL----QAG-----YRVRGTVRSMEKADELIRL------ 46 Query: 72 KNERPNALNKIKAIQGDVLFEDFGLSNLEIENLSKEVTVVFHFAATLRLEAPLKDN---- 127 P +KI+ V+ +D SN + + K+V ++ H A+ +E + DN Sbjct: 47 ---NPGLKDKIEF----VIVKDVSASN-AFDGVLKDVELICHIASPFFVE-NVTDNKSQL 97 Query: 128 VNMNTSGTLRTIKVAKKLKNLQIFVHLSTAFCYPDYEV 165 ++ GTL ++ A+ +K+++ V S+ ++++ Sbjct: 98 LDPAVKGTLGILEAAQGVKSIKRIVITSSFAAVGNFQI 135 >SPAC13F5.06c |sec10||exocyst complex subunit Sec10|Schizosaccharomyces pombe|chr 1|||Manual Length = 811 Score = 27.9 bits (59), Expect = 2.9 Identities = 20/71 (28%), Positives = 37/71 (52%), Gaps = 3/71 (4%) Query: 79 LNKIKAIQGDVLFEDFGLSNLEIENL--SKEVTVVFHFAATLRLEAPLKDNVNMNTSGTL 136 LN++K+ GD + D+ ++ +++N K + FA L L L+DNVN SG + Sbjct: 65 LNRLKSRVGDRI-RDYASASKQVQNEYHQKSNHLREKFAQVLELSRHLEDNVNDMRSGLV 123 Query: 137 RTIKVAKKLKN 147 + ++ +N Sbjct: 124 DAGQELERAEN 134 >SPAC12D12.09 |rev7||DNA polymerase zeta Rev7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 213 Score = 27.5 bits (58), Expect = 3.9 Identities = 12/31 (38%), Positives = 18/31 (58%) Query: 295 EGTKKEKSLTIPVYNVNIGHQKPMTWGDVLT 325 + T++ K +TIPV NV+IG W + T Sbjct: 180 DATRQFKGITIPVRNVDIGPMMTEIWVEKYT 210 >SPBC685.02 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 409 Score = 27.5 bits (58), Expect = 3.9 Identities = 18/64 (28%), Positives = 30/64 (46%), Gaps = 2/64 (3%) Query: 67 LFDRLKNERPNALNKIKAIQGDVLFEDFGLS-NLEIENLSKEVTVVFHFAATLRLEAPLK 125 + DR E P AL ++ ++G +L + G++ I KE + +F + L P K Sbjct: 127 VLDRTSKEEPWALRLLRQLEGIMLLQKNGITREFPIWGYYKE-SSIFGIIDEISLNNPSK 185 Query: 126 DNVN 129 N N Sbjct: 186 SNFN 189 >SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1316 Score = 27.5 bits (58), Expect = 3.9 Identities = 25/69 (36%), Positives = 35/69 (50%), Gaps = 12/69 (17%) Query: 32 KLLYSVPEI-GNVYILMRPKKAKTVAQRLEDLQRLPLFDRLKNERPNALNKIKAIQGDVL 90 K LY++PE +YI VA LE L RL + R + +P+ N + +I G + Sbjct: 655 KSLYTIPESWAKIYIA-------EVALGLEHLHRLGIIHR--DIKPD--NILMSITGHLK 703 Query: 91 FEDFGLSNL 99 DFGLS L Sbjct: 704 LADFGLSQL 712 >SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr 1|||Manual Length = 255 Score = 27.1 bits (57), Expect = 5.1 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 2/49 (4%) Query: 13 GRNILVTGGTGFMGKVLVEKLLYSVPEIGNVYILMRPKKAKTVAQRLED 61 G+ L+TGG+G +G + + + +G +Y R KKA A L D Sbjct: 9 GKTTLITGGSGGIGFSIAKAFAAAGSNVGLLY--GRNKKALEYAAELRD 55 >SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2176 Score = 26.6 bits (56), Expect = 6.7 Identities = 23/77 (29%), Positives = 35/77 (45%), Gaps = 4/77 (5%) Query: 178 EHVMRLIEWLDDRQLTLLTPSLLGPHPNCYTFSKRLAENVVEQAYKDLPIVIARPSIVCP 237 E L ++ DD + T LL P P CY F K +++ + K +P+ R I+ Sbjct: 1275 EQFFLLKKYKDDEHIVNFTVPLLEPLPPCY-FIKIVSDRWLHSITK-VPLSFQR-LIMPE 1331 Query: 238 SYSEPMPGWVDNLNGPV 254 + P P +D N PV Sbjct: 1332 KFPAPTP-LLDLQNAPV 1347 >SPBC839.16 |||C-1-tetrahydrofolate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 937 Score = 26.6 bits (56), Expect = 6.7 Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 2/57 (3%) Query: 210 SKRLAENVVEQAYKDLPIVIARPSIVCPSYSEPMPGWVDNLNGPVGVM-IGAGKGVI 265 +K E +Q Y +LPI IA+ S+ + G N P+ M + AG G I Sbjct: 848 AKERLETFTKQGYNNLPICIAKTQYSL-SHDPDLKGAPTNFTVPIRDMRLSAGAGFI 903 >SPBC725.06c |ppk31|mug25|serine/threonine protein kinase Ppk31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1032 Score = 26.2 bits (55), Expect = 8.9 Identities = 14/41 (34%), Positives = 20/41 (48%) Query: 67 LFDRLKNERPNALNKIKAIQGDVLFEDFGLSNLEIENLSKE 107 +F+ L RP N +KAI + D G + E+ SKE Sbjct: 806 IFEMLTGTRPFEANTVKAIWARIERNDIGWTKRVKESCSKE 846 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.323 0.140 0.432 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,002,080 Number of Sequences: 5004 Number of extensions: 87238 Number of successful extensions: 221 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 10 Number of HSP's that attempted gapping in prelim test: 214 Number of HSP's gapped (non-prelim): 17 length of query: 432 length of database: 2,362,478 effective HSP length: 75 effective length of query: 357 effective length of database: 1,987,178 effective search space: 709422546 effective search space used: 709422546 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 55 (26.2 bits)
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