Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000629-TA|BGIBMGA000629-PA|IPR011129|Cold shock protein,
IPR002059|Cold-shock protein, DNA-binding, IPR008994|Nucleic
acid-binding, OB-fold
(776 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_53793| Best HMM Match : No HMM Matches (HMM E-Value=.) 297 2e-80
SB_31618| Best HMM Match : No HMM Matches (HMM E-Value=.) 40 0.006
SB_4130| Best HMM Match : Cadherin (HMM E-Value=9.3e-29) 33 1.2
SB_34627| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 1.5
SB_21753| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 4.6
SB_19076| Best HMM Match : HECT (HMM E-Value=0) 30 8.1
>SB_53793| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 833
Score = 297 bits (729), Expect = 2e-80
Identities = 188/491 (38%), Positives = 273/491 (55%), Gaps = 34/491 (6%)
Query: 38 RNGDPVEFEMTYDRRTGKPIASTVTKIAPEVVLSEARXXXXXXXXXXXXXXXDNTGRISY 97
R GD VEF ++ D+R+ KP+A + + P V E D R+
Sbjct: 93 RIGDEVEFGVSCDQRSKKPVAVNIVLLTPGTVSFEI------CSEEQVTGIVDAEARLLK 146
Query: 98 ETRGECFFLPYTKDDVEG-NVTLRTGDAVSFQIATNQR-GTLGACHIRFENPVHPVKYHG 155
R ECF+L ++ +D + N+ + GD V F ++ ++R G A + KY G
Sbjct: 147 PGRSECFYLSFSANDCKDPNMPIHKGDKVQFVVSIDKRTGVSRAKDVMLLETAKAEKYQG 206
Query: 156 VVCSMKENFGFIERADVVKEIFFHFSETKCK-EELTLGDDVEFIIQTRNGKEVACNITKL 214
VV SMKE+FGFIERAD V EIFFH+SE ELTLGDDVEFIIQ RN C +
Sbjct: 207 VVSSMKESFGFIERADKVSEIFFHYSEFLDDINELTLGDDVEFIIQPRN----LCALRSY 262
Query: 215 PSGSVVFEDVSPEIMRGQVLKPLERGNLVRATQNDPLPGRIRYRAADHSEVEVPFGDKDQ 274
G+V FEDVS + +G + +PL + + R Q+DPL GRI + D ++ F ++D
Sbjct: 263 LRGTVTFEDVSEKRYQGVIDRPLSKSSAKR--QHDPLHGRIVFET-DDGPSDIVFSERDV 319
Query: 275 CGEFTLRHGDWVQFQVATDRRDQLKRATNISLLDESFYVSGERREQGLVCSLREGFGFIR 334
GEF+LR D V F +A DRRD L+RATNI+L+ + G+ RE G+V +L++GFGFIR
Sbjct: 320 SGEFSLRTKDIVDFNIAVDRRDSLRRATNITLVKVAEPEKGDNREMGVVAALKDGFGFIR 379
Query: 335 CVEREQTMFFHFAEVLRLGQDLTVGDEVEFTVDHLSTFSNMNTRQSAIRIKHLSPGTVQF 394
C +R+ MFFHF+E++ + + +GDEVEF++ + +N R +AI+++ L GT+QF
Sbjct: 380 CCDRDARMFFHFSELVESSRQIQIGDEVEFSI---TEDANSMKRVNAIKVRILPKGTIQF 436
Query: 395 -ETLIERGVRGVVTKEAQVYNNTGSPTRQPPPDT-NGLISCQINQTKKSLPF-MAKKCES 451
+T E +G V +E SP +Q D+ +GLI + KK + F + K +
Sbjct: 437 GDTTTEEIYQGYVDREPMFTGR--SPRKQDSRDSESGLICYEDKGVKKMVSFDIQKNSDI 494
Query: 452 KMLPRVGDKVTFDLYQVKRTKELVAMSVQVQHSLTXXXXXXXXXXRPIMQGFIAALKDGF 511
+ P+ GD V F+L + +RT A ++ V T GF+A LK+ +
Sbjct: 495 RAAPQFGDLVKFNLTESQRTGFRRAANIVVVTRNTDIK----------RYGFVATLKEHY 544
Query: 512 GFIETSDHTKE 522
GF+ETSDH KE
Sbjct: 545 GFLETSDHDKE 555
Score = 151 bits (366), Expect = 2e-36
Identities = 159/531 (29%), Positives = 247/531 (46%), Gaps = 64/531 (12%)
Query: 163 NFGFIERADVVKEIFFHFSETKCK-EELTLGDDVEFIIQT--RNGKEVACNITKLPSGSV 219
++GFI+ + IFFH+S+ K ++L +GD+VEF + R+ K VA NI L G+V
Sbjct: 65 SYGFIQCCERELRIFFHYSQYKGDLDDLRIGDEVEFGVSCDQRSKKPVAVNIVLLTPGTV 124
Query: 220 VFEDVSPEIMRGQVLKPLERGNLVRATQNDPLPGRIRYRAADHSEVEVPFGDKDQCGEFT 279
FE S E + G V L++ +++ + + A D + +P
Sbjct: 125 SFEICSEEQVTGIVDA---EARLLKPGRSECF--YLSFSANDCKDPNMP----------- 168
Query: 280 LRHGDWVQFQVATDRRDQLKRATNISLLDESFYVSGERREQGLVCSLREGFGFIRCVERE 339
+ GD VQF V+ D+R + RA ++ LL+ + + QG+V S++E FGFI ++
Sbjct: 169 IHKGDKVQFVVSIDKRTGVSRAKDVMLLE----TAKAEKYQGVVSSMKESFGFIERADKV 224
Query: 340 QTMFFHFAEVLRLGQDLTVGDEVEFTVDHLSTFSNMNTRQSAIRIKHLSPGTVQFETLIE 399
+FFH++E L +LT+GD+VEF + N+ +S +R GTV FE + E
Sbjct: 225 SEIFFHYSEFLDDINELTLGDDVEFIIQP----RNLCALRSYLR------GTVTFEDVSE 274
Query: 400 RGVRGVVTKEAQVYNNTGSPTRQPPPDTNGLISCQINQTKKSLPFMAKKCESKMLPRVGD 459
+ +GV+ + + S RQ P +G I + + + F + + R D
Sbjct: 275 KRYQGVIDRPL----SKSSAKRQHDP-LHGRIVFETDDGPSDIVFSERDVSGEFSLRTKD 329
Query: 460 KVTFDLYQVKRTKELVAMSVQVQHSLTXXXXXXXXXXRPIMQGFIAALKDGFGFIETSDH 519
V F++ +R A ++ +L R + G +AALKDGFGFI D
Sbjct: 330 IVDFNIAVDRRDSLRRATNI----TLVKVAEPEKGDNREM--GVVAALKDGFGFIRCCDR 383
Query: 520 TKEVFFHFSNLEGSPENLELGIEVEYSLGRLNGSGGGCASAEYVRVLARGTIPIA-KPLE 578
+FFHFS L S +++G EVE+S+ + + +A VR+L +GTI E
Sbjct: 384 DARMFFHFSELVESSRQIQIGDEVEFSITE-DANSMKRVNAIKVRILPKGTIQFGDTTTE 442
Query: 579 PTLNGTVTR----TLRALNPDQAQ--YSGLIQVEG-GTS--YEFGIMGLSCKREILQVGD 629
G V R T R+ ++ SGLI E G F I S R Q GD
Sbjct: 443 EIYQGYVDREPMFTGRSPRKQDSRDSESGLICYEDKGVKKMVSFDIQKNSDIRAAPQFGD 502
Query: 630 PVTF---QADIEG--RASNIVPIRK----KRRAIVDAIKGVFGFLSVDGED 671
V F ++ G RA+NIV + + KR V +K +GFL D
Sbjct: 503 LVKFNLTESQRTGFRRAANIVVVTRNTDIKRYGFVATLKEHYGFLETSDHD 553
Score = 117 bits (281), Expect = 4e-26
Identities = 120/412 (29%), Positives = 179/412 (43%), Gaps = 62/412 (15%)
Query: 330 FGFIRCVEREQTMFFHFAEVLRLGQDLTVGDEVEFTVDHLSTFSNMNTRQSAIRIKHLSP 389
+GFI+C ERE +FFH+++ DL +GDEVEF V + + + A+ I L+P
Sbjct: 66 YGFIQCCERELRIFFHYSQYKGDLDDLRIGDEVEFGV----SCDQRSKKPVAVNIVLLTP 121
Query: 390 GTVQFETLIERGVRGVVTKEAQVYNNTGSPTRQPPPDTNGLISCQINQTKKSLPFMAKKC 449
GTV FE E V G+V EA++ P R C L F A C
Sbjct: 122 GTVSFEICSEEQVTGIVDAEARLLK----PGRS---------ECFY------LSFSANDC 162
Query: 450 ESKMLP-RVGDKVTFDLYQVKRTKELVAMSVQVQHSLTXXXXXXXXXXRPIMQGFIAALK 508
+ +P GDKV F + KRT A V + + QG ++++K
Sbjct: 163 KDPNMPIHKGDKVQFVVSIDKRTGVSRAKDVMLLETAKAEKY----------QGVVSSMK 212
Query: 509 DGFGFIETSDHTKEVFFHFSNLEGSPENLELGIEVEYSLGRLNGSGGGCASAEYVRVLAR 568
+ FGFIE +D E+FFH+S L LG +VE+ + N CA Y+R
Sbjct: 213 ESFGFIERADKVSEIFFHYSEFLDDINELTLGDDVEFIIQPRNL----CALRSYLR---- 264
Query: 569 GTIPIAKPLEPTLNGTVTRTLRALNPDQAQ--YSGLIQVE---GGTSYEFGIMGLSCKRE 623
GT+ E G + R L + + G I E G + F +S +
Sbjct: 265 GTVTFEDVSEKRYQGVIDRPLSKSSAKRQHDPLHGRIVFETDDGPSDIVFSERDVSGEFS 324
Query: 624 ILQVGDPVTFQADIEGR-----ASNIVPIR--------KKRRAIVDAIKGVFGFLSVDGE 670
L+ D V F ++ R A+NI ++ + +V A+K FGF+
Sbjct: 325 -LRTKDIVDFNIAVDRRDSLRRATNITLVKVAEPEKGDNREMGVVAALKDGFGFIRCCDR 383
Query: 671 DTRRLFFHMSEVRGNPSELQSGDTVEFVMLTNPRNGKSSACNVVKIASKGKV 722
D R +FFH SE+ + ++Q GD VEF + + + K V+I KG +
Sbjct: 384 DAR-MFFHFSELVESSRQIQIGDEVEFSITEDANSMKRVNAIKVRILPKGTI 434
Score = 52.8 bits (121), Expect = 1e-06
Identities = 29/55 (52%), Positives = 36/55 (65%), Gaps = 2/55 (3%)
Query: 662 FGFLSVDGEDTRRLFFHMSEVRGNPSELQSGDTVEFVMLTNPRNGKSSACNVVKI 716
FGF+ + E+ + LFFH+SEV N ELQ+GD VEF + R GK SA NV KI
Sbjct: 718 FGFIGHENEEGKNLFFHISEVAEN-VELQAGDEVEF-FVVQKRGGKLSAVNVRKI 770
Score = 48.8 bits (111), Expect = 2e-05
Identities = 28/74 (37%), Positives = 43/74 (58%), Gaps = 3/74 (4%)
Query: 160 MKENFGFI-ERADVVKEIFFHFSETKCKEELTLGDDVE-FIIQTRNGKEVACNITKLPSG 217
++ +FGFI + K +FFH SE EL GD+VE F++Q R GK A N+ K+
Sbjct: 714 LRRHFGFIGHENEEGKNLFFHISEVAENVELQAGDEVEFFVVQKRGGKLSAVNVRKI-RV 772
Query: 218 SVVFEDVSPEIMRG 231
V+ + ++P+ RG
Sbjct: 773 MVIRKPIAPDGTRG 786
Score = 40.3 bits (90), Expect = 0.006
Identities = 25/64 (39%), Positives = 37/64 (57%), Gaps = 6/64 (9%)
Query: 610 SYEFGIMGLSCKREILQVGDPVTFQADIE-----GRASNIVPIRKKRRAIVDAIKGVFGF 664
S E+GI L K+ +LQ GD V FQ ++ RA + P R++ RA V+++KG
Sbjct: 588 SVEYGITSLIDKKTVLQCGDRVRFQIGVDRETGCKRAMKVTPERQRVRARVESVKGQLS- 646
Query: 665 LSVD 668
LSV+
Sbjct: 647 LSVE 650
Score = 34.7 bits (76), Expect = 0.29
Identities = 28/87 (32%), Positives = 43/87 (49%), Gaps = 9/87 (10%)
Query: 507 LKDGFGFI-ETSDHTKEVFFHFSNLEGSPENLELGIEVEYSLGRLNGSGGGCASAEYVRV 565
L+ FGFI ++ K +FFH S + + E L+ G EVE+ + + G + +RV
Sbjct: 714 LRRHFGFIGHENEEGKNLFFHISEVAENVE-LQAGDEVEFFVVQKRGGKLSAVNVRKIRV 772
Query: 566 LARGTIPIAKPLEP--TLNGTVTRTLR 590
+ I KP+ P T T R+LR
Sbjct: 773 MV-----IRKPIAPDGTRGFTYQRSLR 794
Score = 33.9 bits (74), Expect = 0.50
Identities = 20/42 (47%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Query: 326 LREGFGFIRCVERE-QTMFFHFAEVLRLGQDLTVGDEVEFTV 366
LR FGFI E + +FFH +EV +L GDEVEF V
Sbjct: 714 LRRHFGFIGHENEEGKNLFFHISEVAE-NVELQAGDEVEFFV 754
>SB_31618| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 45
Score = 40.3 bits (90), Expect = 0.006
Identities = 17/42 (40%), Positives = 26/42 (61%)
Query: 318 REQGLVCSLREGFGFIRCVEREQTMFFHFAEVLRLGQDLTVG 359
RE G++ L +GFI+C ERE +FFH+++ DL +G
Sbjct: 3 RETGIIEKLLHSYGFIQCCERELRIFFHYSQYKGDLDDLRIG 44
Score = 34.3 bits (75), Expect = 0.38
Identities = 13/39 (33%), Positives = 22/39 (56%)
Query: 502 GFIAALKDGFGFIETSDHTKEVFFHFSNLEGSPENLELG 540
G I L +GFI+ + +FFH+S +G ++L +G
Sbjct: 6 GIIEKLLHSYGFIQCCERELRIFFHYSQYKGDLDDLRIG 44
>SB_4130| Best HMM Match : Cadherin (HMM E-Value=9.3e-29)
Length = 322
Score = 32.7 bits (71), Expect = 1.2
Identities = 16/38 (42%), Positives = 21/38 (55%)
Query: 25 DLQHSNMQHKSSYRNGDPVEFEMTYDRRTGKPIASTVT 62
DLQ S + +S R G V+ E+ R G PI ST+T
Sbjct: 200 DLQFSRKNYSASVREGVSVDSELVTVRALGYPIGSTIT 237
>SB_34627| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1925
Score = 32.3 bits (70), Expect = 1.5
Identities = 17/36 (47%), Positives = 22/36 (61%), Gaps = 3/36 (8%)
Query: 546 SLGRLNGSGGGCASA---EYVRVLARGTIPIAKPLE 578
SL RL GS GGC + EY+R +A P+A P+E
Sbjct: 1109 SLLRLTGSKGGCINTDAEEYIRHVAEMAAPVAIPIE 1144
>SB_21753| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 276
Score = 30.7 bits (66), Expect = 4.6
Identities = 24/78 (30%), Positives = 43/78 (55%), Gaps = 3/78 (3%)
Query: 173 VKEIFFHFSETKCKEE-LTLGDDVEFIIQTRNG-KEVACNITKLPSGSVVFEDVSPEIMR 230
++E +HF+E+K ++ L + D V +++ + G K V I + SV F +S + +R
Sbjct: 65 IEEFGYHFNESKFEDNLLVITDHVYKLLEEKAGLKRVYVPIDPRENESVGFIFMSEDALR 124
Query: 231 GQVLKPLERGN-LVRATQ 247
+ L L G+ +VRA Q
Sbjct: 125 AEKLMILIHGSGVVRAGQ 142
>SB_19076| Best HMM Match : HECT (HMM E-Value=0)
Length = 2018
Score = 29.9 bits (64), Expect = 8.1
Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 4/64 (6%)
Query: 11 QPPDSHSSPLQDILDLQ-HSNMQHKSSYRNGDPVEFEMTYDRRTGKPIASTVTKIAPEVV 69
+PPD ++PL++ L L ++ H ++ R F DR GK + T+ P+V
Sbjct: 1365 EPPDPFNTPLEEALPLACQPHLLHPNARRE---QLFGAGQDRHKGKQASRTMALSRPDVT 1421
Query: 70 LSEA 73
+++A
Sbjct: 1422 VTQA 1425
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.319 0.136 0.398
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,270,477
Number of Sequences: 59808
Number of extensions: 1039546
Number of successful extensions: 1991
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1942
Number of HSP's gapped (non-prelim): 25
length of query: 776
length of database: 16,821,457
effective HSP length: 88
effective length of query: 688
effective length of database: 11,558,353
effective search space: 7952146864
effective search space used: 7952146864
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 64 (29.9 bits)
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