BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000621-TA|BGIBMGA000621-PA|IPR000886|Endoplasmic reticulum targeting sequence, IPR002654|Glycosyl transferase, family 25 (402 letters) Database: fruitfly 52,641 sequences; 24,830,863 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AE014134-928|AAN10543.1| 612|Drosophila melanogaster CG31915-PA... 196 6e-50 BT003764-1|AAO41443.1| 595|Drosophila melanogaster RE30068p pro... 34 0.40 AE014296-1928|AAN11883.1| 721|Drosophila melanogaster CG6199-PB... 34 0.40 AE014296-1927|AAF50079.1| 721|Drosophila melanogaster CG6199-PA... 34 0.40 AY051748-1|AAK93172.1| 662|Drosophila melanogaster LD27655p pro... 31 2.1 AE013599-75|AAM68379.1| 662|Drosophila melanogaster CG10417-PB,... 31 2.1 AE013599-74|AAF57333.1| 662|Drosophila melanogaster CG10417-PA,... 31 2.1 AY089486-1|AAL90224.2| 205|Drosophila melanogaster AT31112p pro... 31 3.7 AE014134-1919|AAF52976.1| 409|Drosophila melanogaster CG7363-PA... 31 3.7 AY058623-1|AAL13852.1| 1139|Drosophila melanogaster LD31543p pro... 29 8.6 AE014298-2841|AAF48954.2| 1139|Drosophila melanogaster CG32533-P... 29 8.6 AE013599-453|AAF59228.1| 3242|Drosophila melanogaster CG2093-PA ... 29 8.6 >AE014134-928|AAN10543.1| 612|Drosophila melanogaster CG31915-PA protein. Length = 612 Score = 196 bits (477), Expect = 6e-50 Identities = 95/215 (44%), Positives = 133/215 (61%), Gaps = 11/215 (5%) Query: 77 CGMTENYYYKRTDDYKPILNRKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITN--- 133 CGMTE+YYY+RTD+YK I + KK G F VPMVH+AVL++M + LT+ +K+ Sbjct: 181 CGMTEDYYYRRTDEYKEIYHVKKQGSFPVPMVHTAVLVNMNHRAVRNLTFDRNKLVELQK 240 Query: 134 -------YDGPEDDIIAFALNSKALGIPLYICNEAIYGFVPVPLEDNDPLEKDYEQMLNI 186 YDGP DDII FA+++ + GIPL+ICN+ +G++ PLE D L+ D +Q++N+ Sbjct: 241 SRQQEPLYDGPADDIIVFAISANSSGIPLHICNDITFGYILQPLEPGDTLDHDVQQLVNL 300 Query: 187 KLEAISQ-GTPLPLNHMLEEYVTHPNKWKFGCDEIYMINLERRQERRFLMELSFKELGMS 245 K +++ G PL + P K K D I+MINL+RR ERR ME F E+G+ Sbjct: 301 KSIMVNELGAVPPLLDYYKHLEKKPEKSKLSLDRIFMINLKRRPERREKMERLFDEIGIE 360 Query: 246 VQHFSAIDGRNLDMNDLREYSITLMPNYEDPYHKR 280 +HF A+DG+ L L E + +P YEDPYH R Sbjct: 361 AEHFPAVDGKELSTERLLEMGVRFLPGYEDPYHHR 395 Score = 132 bits (319), Expect = 8e-31 Identities = 64/113 (56%), Positives = 81/113 (71%), Gaps = 4/113 (3%) Query: 277 YHKRYIGRKILLDGEEEYATPHTTRPL---YSYWTLGYLISERGAKKLLRADPLSKMLPV 333 Y Y GRK L + E E A + + YSYWTLGY+IS +GA KLL A PL K++PV Sbjct: 453 YDLIYFGRKRLKE-ESEPAVENADNLVHAGYSYWTLGYVISLQGALKLLAAKPLDKLIPV 511 Query: 334 DEFLPIMFDQHPNEEWKSHFENRDLIALSAAPLLVHPTHYTGQPGYISDTEDS 386 DEFLP+MFD+HPN+ W F R+L+A SA+PLL++P +YTG+ GYISDTEDS Sbjct: 512 DEFLPLMFDRHPNKTWTEAFPKRNLVAFSASPLLLYPIYYTGESGYISDTEDS 564 Score = 41.9 bits (94), Expect = 0.002 Identities = 18/71 (25%), Positives = 36/71 (50%), Gaps = 1/71 (1%) Query: 6 IHSDFNEDKSIEIINKWVENYGSEYNGMYVTTNTSSGPLHSDESSSTNWSPNHFRHVINM 65 + D + D SIE++ +W++N G Y+ + + +S W + F+H+I + Sbjct: 66 LRCDHSNDDSIELLRQWLDNSGDLYHSVSYEFKPEEQSF-VNGTSPYEWPASRFKHLIAL 124 Query: 66 REAALDFGRKL 76 +E A +GR + Sbjct: 125 KEEAFQYGRDI 135 >BT003764-1|AAO41443.1| 595|Drosophila melanogaster RE30068p protein. Length = 595 Score = 33.9 bits (74), Expect = 0.40 Identities = 26/86 (30%), Positives = 44/86 (51%), Gaps = 9/86 (10%) Query: 79 MTENYYYKRTDDYKPILNRKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPE 138 ++E YY R+ DY I+ R+ G F+VP V S L+ + D +++ K +D P+ Sbjct: 419 LSEGGYYARSHDYVDIVKRELIGMFNVPHVTSIYLV--KKTAFDAISF---KHKEFD-PD 472 Query: 139 DDIIAFALNSKALGIPLYICNEAIYG 164 +A + + GI +Y N I+G Sbjct: 473 ---MAMCESLRNAGIFMYASNLRIFG 495 >AE014296-1928|AAN11883.1| 721|Drosophila melanogaster CG6199-PB, isoform B protein. Length = 721 Score = 33.9 bits (74), Expect = 0.40 Identities = 26/86 (30%), Positives = 44/86 (51%), Gaps = 9/86 (10%) Query: 79 MTENYYYKRTDDYKPILNRKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPE 138 ++E YY R+ DY I+ R+ G F+VP V S L+ + D +++ K +D P+ Sbjct: 419 LSEGGYYARSHDYVDIVKRELIGMFNVPHVTSIYLV--KKTAFDAISF---KHKEFD-PD 472 Query: 139 DDIIAFALNSKALGIPLYICNEAIYG 164 +A + + GI +Y N I+G Sbjct: 473 ---MAMCESLRNAGIFMYASNLRIFG 495 >AE014296-1927|AAF50079.1| 721|Drosophila melanogaster CG6199-PA, isoform A protein. Length = 721 Score = 33.9 bits (74), Expect = 0.40 Identities = 26/86 (30%), Positives = 44/86 (51%), Gaps = 9/86 (10%) Query: 79 MTENYYYKRTDDYKPILNRKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPE 138 ++E YY R+ DY I+ R+ G F+VP V S L+ + D +++ K +D P+ Sbjct: 419 LSEGGYYARSHDYVDIVKRELIGMFNVPHVTSIYLV--KKTAFDAISF---KHKEFD-PD 472 Query: 139 DDIIAFALNSKALGIPLYICNEAIYG 164 +A + + GI +Y N I+G Sbjct: 473 ---MAMCESLRNAGIFMYASNLRIFG 495 >AY051748-1|AAK93172.1| 662|Drosophila melanogaster LD27655p protein. Length = 662 Score = 31.5 bits (68), Expect = 2.1 Identities = 28/125 (22%), Positives = 56/125 (44%), Gaps = 6/125 (4%) Query: 89 DDYKPILN-RKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPEDDIIAFALN 147 D + ILN T F V H ++ +Y +DKL ++ + Y + ++ AL Sbjct: 38 DAHNSILNFDNNTSFFAVYDGHGGAEVA-QY-CADKLPHFLKNLETYKNGQFEV---ALK 92 Query: 148 SKALGIPLYICNEAIYGFVPVPLEDNDPLEKDYEQMLNIKLEAISQGTPLPLNHMLEEYV 207 LG + + +I + + +++ ++ + + L + + + LPLN +LE+Y Sbjct: 93 EAFLGFDKTLLDPSIVSILKILAGEHNFVDAEADDYEEEDLAELQEESNLPLNEVLEKYK 152 Query: 208 THPNK 212 P K Sbjct: 153 GLPQK 157 >AE013599-75|AAM68379.1| 662|Drosophila melanogaster CG10417-PB, isoform B protein. Length = 662 Score = 31.5 bits (68), Expect = 2.1 Identities = 28/125 (22%), Positives = 56/125 (44%), Gaps = 6/125 (4%) Query: 89 DDYKPILN-RKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPEDDIIAFALN 147 D + ILN T F V H ++ +Y +DKL ++ + Y + ++ AL Sbjct: 38 DAHNSILNFDNNTSFFAVYDGHGGAEVA-QY-CADKLPHFLKNLETYKNGQFEV---ALK 92 Query: 148 SKALGIPLYICNEAIYGFVPVPLEDNDPLEKDYEQMLNIKLEAISQGTPLPLNHMLEEYV 207 LG + + +I + + +++ ++ + + L + + + LPLN +LE+Y Sbjct: 93 EAFLGFDKTLLDPSIVSILKILAGEHNFVDAEADDYEEEDLAELQEESNLPLNEVLEKYK 152 Query: 208 THPNK 212 P K Sbjct: 153 GLPQK 157 >AE013599-74|AAF57333.1| 662|Drosophila melanogaster CG10417-PA, isoform A protein. Length = 662 Score = 31.5 bits (68), Expect = 2.1 Identities = 28/125 (22%), Positives = 56/125 (44%), Gaps = 6/125 (4%) Query: 89 DDYKPILN-RKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPEDDIIAFALN 147 D + ILN T F V H ++ +Y +DKL ++ + Y + ++ AL Sbjct: 38 DAHNSILNFDNNTSFFAVYDGHGGAEVA-QY-CADKLPHFLKNLETYKNGQFEV---ALK 92 Query: 148 SKALGIPLYICNEAIYGFVPVPLEDNDPLEKDYEQMLNIKLEAISQGTPLPLNHMLEEYV 207 LG + + +I + + +++ ++ + + L + + + LPLN +LE+Y Sbjct: 93 EAFLGFDKTLLDPSIVSILKILAGEHNFVDAEADDYEEEDLAELQEESNLPLNEVLEKYK 152 Query: 208 THPNK 212 P K Sbjct: 153 GLPQK 157 >AY089486-1|AAL90224.2| 205|Drosophila melanogaster AT31112p protein. Length = 205 Score = 30.7 bits (66), Expect = 3.7 Identities = 16/57 (28%), Positives = 27/57 (47%) Query: 30 YNGMYVTTNTSSGPLHSDESSSTNWSPNHFRHVINMREAALDFGRKLCGMTENYYYK 86 + GM ++ ++ + S+S+ P I R A DF RK+ + ENY Y+ Sbjct: 101 WKGMLLSVTHTTLSTSTSHSTSSGKPPKSAVQAIPQRTAKADFKRKMYVLKENYNYR 157 >AE014134-1919|AAF52976.1| 409|Drosophila melanogaster CG7363-PA protein. Length = 409 Score = 30.7 bits (66), Expect = 3.7 Identities = 16/57 (28%), Positives = 27/57 (47%) Query: 30 YNGMYVTTNTSSGPLHSDESSSTNWSPNHFRHVINMREAALDFGRKLCGMTENYYYK 86 + GM ++ ++ + S+S+ P I R A DF RK+ + ENY Y+ Sbjct: 305 WKGMLLSVTHTTLSTSTSHSTSSGKPPKSAVQAIPQRTAKADFKRKMYVLKENYNYR 361 >AY058623-1|AAL13852.1| 1139|Drosophila melanogaster LD31543p protein. Length = 1139 Score = 29.5 bits (63), Expect = 8.6 Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 3/32 (9%) Query: 343 QHPNEEWKSHFENRDLIALSAAPLLVHPTHYT 374 QHPN ++ HFE L+ L+ + LL P YT Sbjct: 825 QHPNSQFAKHFE---LLKLTESDLLPKPDFYT 853 >AE014298-2841|AAF48954.2| 1139|Drosophila melanogaster CG32533-PA protein. Length = 1139 Score = 29.5 bits (63), Expect = 8.6 Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 3/32 (9%) Query: 343 QHPNEEWKSHFENRDLIALSAAPLLVHPTHYT 374 QHPN ++ HFE L+ L+ + LL P YT Sbjct: 825 QHPNSQFAKHFE---LLKLTESDLLPKPDFYT 853 >AE013599-453|AAF59228.1| 3242|Drosophila melanogaster CG2093-PA protein. Length = 3242 Score = 29.5 bits (63), Expect = 8.6 Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 9/57 (15%) Query: 313 ISERGAKKLLRADPLSKMLPVDEFLPIMFDQHPNEEWKSHFENRDLIALSAAPLLVH 369 +S+ A K R D S LP+ F F++H EE KS ++N L PL +H Sbjct: 2792 VSDEAAAKQFRQDVESIELPLSAF----FEEHSLEEQKSFYDN-----LHLGPLKIH 2839 Database: fruitfly Posted date: Oct 5, 2007 11:13 AM Number of letters in database: 24,830,863 Number of sequences in database: 52,641 Lambda K H 0.318 0.137 0.419 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,039,689 Number of Sequences: 52641 Number of extensions: 1017469 Number of successful extensions: 2369 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 2358 Number of HSP's gapped (non-prelim): 14 length of query: 402 length of database: 24,830,863 effective HSP length: 87 effective length of query: 315 effective length of database: 20,251,096 effective search space: 6379095240 effective search space used: 6379095240 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits) S2: 63 (29.5 bits)
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