SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000621-TA|BGIBMGA000621-PA|IPR000886|Endoplasmic
reticulum targeting sequence, IPR002654|Glycosyl transferase, family
25
         (402 letters)

Database: fruitfly 
           52,641 sequences; 24,830,863 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014134-928|AAN10543.1|  612|Drosophila melanogaster CG31915-PA...   196   6e-50
BT003764-1|AAO41443.1|  595|Drosophila melanogaster RE30068p pro...    34   0.40 
AE014296-1928|AAN11883.1|  721|Drosophila melanogaster CG6199-PB...    34   0.40 
AE014296-1927|AAF50079.1|  721|Drosophila melanogaster CG6199-PA...    34   0.40 
AY051748-1|AAK93172.1|  662|Drosophila melanogaster LD27655p pro...    31   2.1  
AE013599-75|AAM68379.1|  662|Drosophila melanogaster CG10417-PB,...    31   2.1  
AE013599-74|AAF57333.1|  662|Drosophila melanogaster CG10417-PA,...    31   2.1  
AY089486-1|AAL90224.2|  205|Drosophila melanogaster AT31112p pro...    31   3.7  
AE014134-1919|AAF52976.1|  409|Drosophila melanogaster CG7363-PA...    31   3.7  
AY058623-1|AAL13852.1| 1139|Drosophila melanogaster LD31543p pro...    29   8.6  
AE014298-2841|AAF48954.2| 1139|Drosophila melanogaster CG32533-P...    29   8.6  
AE013599-453|AAF59228.1| 3242|Drosophila melanogaster CG2093-PA ...    29   8.6  

>AE014134-928|AAN10543.1|  612|Drosophila melanogaster CG31915-PA
           protein.
          Length = 612

 Score =  196 bits (477), Expect = 6e-50
 Identities = 95/215 (44%), Positives = 133/215 (61%), Gaps = 11/215 (5%)

Query: 77  CGMTENYYYKRTDDYKPILNRKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITN--- 133
           CGMTE+YYY+RTD+YK I + KK G F VPMVH+AVL++M +     LT+  +K+     
Sbjct: 181 CGMTEDYYYRRTDEYKEIYHVKKQGSFPVPMVHTAVLVNMNHRAVRNLTFDRNKLVELQK 240

Query: 134 -------YDGPEDDIIAFALNSKALGIPLYICNEAIYGFVPVPLEDNDPLEKDYEQMLNI 186
                  YDGP DDII FA+++ + GIPL+ICN+  +G++  PLE  D L+ D +Q++N+
Sbjct: 241 SRQQEPLYDGPADDIIVFAISANSSGIPLHICNDITFGYILQPLEPGDTLDHDVQQLVNL 300

Query: 187 KLEAISQ-GTPLPLNHMLEEYVTHPNKWKFGCDEIYMINLERRQERRFLMELSFKELGMS 245
           K   +++ G   PL    +     P K K   D I+MINL+RR ERR  ME  F E+G+ 
Sbjct: 301 KSIMVNELGAVPPLLDYYKHLEKKPEKSKLSLDRIFMINLKRRPERREKMERLFDEIGIE 360

Query: 246 VQHFSAIDGRNLDMNDLREYSITLMPNYEDPYHKR 280
            +HF A+DG+ L    L E  +  +P YEDPYH R
Sbjct: 361 AEHFPAVDGKELSTERLLEMGVRFLPGYEDPYHHR 395



 Score =  132 bits (319), Expect = 8e-31
 Identities = 64/113 (56%), Positives = 81/113 (71%), Gaps = 4/113 (3%)

Query: 277 YHKRYIGRKILLDGEEEYATPHTTRPL---YSYWTLGYLISERGAKKLLRADPLSKMLPV 333
           Y   Y GRK L + E E A  +    +   YSYWTLGY+IS +GA KLL A PL K++PV
Sbjct: 453 YDLIYFGRKRLKE-ESEPAVENADNLVHAGYSYWTLGYVISLQGALKLLAAKPLDKLIPV 511

Query: 334 DEFLPIMFDQHPNEEWKSHFENRDLIALSAAPLLVHPTHYTGQPGYISDTEDS 386
           DEFLP+MFD+HPN+ W   F  R+L+A SA+PLL++P +YTG+ GYISDTEDS
Sbjct: 512 DEFLPLMFDRHPNKTWTEAFPKRNLVAFSASPLLLYPIYYTGESGYISDTEDS 564



 Score = 41.9 bits (94), Expect = 0.002
 Identities = 18/71 (25%), Positives = 36/71 (50%), Gaps = 1/71 (1%)

Query: 6   IHSDFNEDKSIEIINKWVENYGSEYNGMYVTTNTSSGPLHSDESSSTNWSPNHFRHVINM 65
           +  D + D SIE++ +W++N G  Y+ +             + +S   W  + F+H+I +
Sbjct: 66  LRCDHSNDDSIELLRQWLDNSGDLYHSVSYEFKPEEQSF-VNGTSPYEWPASRFKHLIAL 124

Query: 66  REAALDFGRKL 76
           +E A  +GR +
Sbjct: 125 KEEAFQYGRDI 135


>BT003764-1|AAO41443.1|  595|Drosophila melanogaster RE30068p
           protein.
          Length = 595

 Score = 33.9 bits (74), Expect = 0.40
 Identities = 26/86 (30%), Positives = 44/86 (51%), Gaps = 9/86 (10%)

Query: 79  MTENYYYKRTDDYKPILNRKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPE 138
           ++E  YY R+ DY  I+ R+  G F+VP V S  L+  +    D +++   K   +D P+
Sbjct: 419 LSEGGYYARSHDYVDIVKRELIGMFNVPHVTSIYLV--KKTAFDAISF---KHKEFD-PD 472

Query: 139 DDIIAFALNSKALGIPLYICNEAIYG 164
              +A   + +  GI +Y  N  I+G
Sbjct: 473 ---MAMCESLRNAGIFMYASNLRIFG 495


>AE014296-1928|AAN11883.1|  721|Drosophila melanogaster CG6199-PB,
           isoform B protein.
          Length = 721

 Score = 33.9 bits (74), Expect = 0.40
 Identities = 26/86 (30%), Positives = 44/86 (51%), Gaps = 9/86 (10%)

Query: 79  MTENYYYKRTDDYKPILNRKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPE 138
           ++E  YY R+ DY  I+ R+  G F+VP V S  L+  +    D +++   K   +D P+
Sbjct: 419 LSEGGYYARSHDYVDIVKRELIGMFNVPHVTSIYLV--KKTAFDAISF---KHKEFD-PD 472

Query: 139 DDIIAFALNSKALGIPLYICNEAIYG 164
              +A   + +  GI +Y  N  I+G
Sbjct: 473 ---MAMCESLRNAGIFMYASNLRIFG 495


>AE014296-1927|AAF50079.1|  721|Drosophila melanogaster CG6199-PA,
           isoform A protein.
          Length = 721

 Score = 33.9 bits (74), Expect = 0.40
 Identities = 26/86 (30%), Positives = 44/86 (51%), Gaps = 9/86 (10%)

Query: 79  MTENYYYKRTDDYKPILNRKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPE 138
           ++E  YY R+ DY  I+ R+  G F+VP V S  L+  +    D +++   K   +D P+
Sbjct: 419 LSEGGYYARSHDYVDIVKRELIGMFNVPHVTSIYLV--KKTAFDAISF---KHKEFD-PD 472

Query: 139 DDIIAFALNSKALGIPLYICNEAIYG 164
              +A   + +  GI +Y  N  I+G
Sbjct: 473 ---MAMCESLRNAGIFMYASNLRIFG 495


>AY051748-1|AAK93172.1|  662|Drosophila melanogaster LD27655p
           protein.
          Length = 662

 Score = 31.5 bits (68), Expect = 2.1
 Identities = 28/125 (22%), Positives = 56/125 (44%), Gaps = 6/125 (4%)

Query: 89  DDYKPILN-RKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPEDDIIAFALN 147
           D +  ILN    T  F V   H    ++ +Y  +DKL ++   +  Y   + ++   AL 
Sbjct: 38  DAHNSILNFDNNTSFFAVYDGHGGAEVA-QY-CADKLPHFLKNLETYKNGQFEV---ALK 92

Query: 148 SKALGIPLYICNEAIYGFVPVPLEDNDPLEKDYEQMLNIKLEAISQGTPLPLNHMLEEYV 207
              LG    + + +I   + +   +++ ++ + +      L  + + + LPLN +LE+Y 
Sbjct: 93  EAFLGFDKTLLDPSIVSILKILAGEHNFVDAEADDYEEEDLAELQEESNLPLNEVLEKYK 152

Query: 208 THPNK 212
             P K
Sbjct: 153 GLPQK 157


>AE013599-75|AAM68379.1|  662|Drosophila melanogaster CG10417-PB,
           isoform B protein.
          Length = 662

 Score = 31.5 bits (68), Expect = 2.1
 Identities = 28/125 (22%), Positives = 56/125 (44%), Gaps = 6/125 (4%)

Query: 89  DDYKPILN-RKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPEDDIIAFALN 147
           D +  ILN    T  F V   H    ++ +Y  +DKL ++   +  Y   + ++   AL 
Sbjct: 38  DAHNSILNFDNNTSFFAVYDGHGGAEVA-QY-CADKLPHFLKNLETYKNGQFEV---ALK 92

Query: 148 SKALGIPLYICNEAIYGFVPVPLEDNDPLEKDYEQMLNIKLEAISQGTPLPLNHMLEEYV 207
              LG    + + +I   + +   +++ ++ + +      L  + + + LPLN +LE+Y 
Sbjct: 93  EAFLGFDKTLLDPSIVSILKILAGEHNFVDAEADDYEEEDLAELQEESNLPLNEVLEKYK 152

Query: 208 THPNK 212
             P K
Sbjct: 153 GLPQK 157


>AE013599-74|AAF57333.1|  662|Drosophila melanogaster CG10417-PA,
           isoform A protein.
          Length = 662

 Score = 31.5 bits (68), Expect = 2.1
 Identities = 28/125 (22%), Positives = 56/125 (44%), Gaps = 6/125 (4%)

Query: 89  DDYKPILN-RKKTGCFDVPMVHSAVLISMRYDVSDKLTYYPSKITNYDGPEDDIIAFALN 147
           D +  ILN    T  F V   H    ++ +Y  +DKL ++   +  Y   + ++   AL 
Sbjct: 38  DAHNSILNFDNNTSFFAVYDGHGGAEVA-QY-CADKLPHFLKNLETYKNGQFEV---ALK 92

Query: 148 SKALGIPLYICNEAIYGFVPVPLEDNDPLEKDYEQMLNIKLEAISQGTPLPLNHMLEEYV 207
              LG    + + +I   + +   +++ ++ + +      L  + + + LPLN +LE+Y 
Sbjct: 93  EAFLGFDKTLLDPSIVSILKILAGEHNFVDAEADDYEEEDLAELQEESNLPLNEVLEKYK 152

Query: 208 THPNK 212
             P K
Sbjct: 153 GLPQK 157


>AY089486-1|AAL90224.2|  205|Drosophila melanogaster AT31112p
           protein.
          Length = 205

 Score = 30.7 bits (66), Expect = 3.7
 Identities = 16/57 (28%), Positives = 27/57 (47%)

Query: 30  YNGMYVTTNTSSGPLHSDESSSTNWSPNHFRHVINMREAALDFGRKLCGMTENYYYK 86
           + GM ++   ++    +  S+S+   P      I  R A  DF RK+  + ENY Y+
Sbjct: 101 WKGMLLSVTHTTLSTSTSHSTSSGKPPKSAVQAIPQRTAKADFKRKMYVLKENYNYR 157


>AE014134-1919|AAF52976.1|  409|Drosophila melanogaster CG7363-PA
           protein.
          Length = 409

 Score = 30.7 bits (66), Expect = 3.7
 Identities = 16/57 (28%), Positives = 27/57 (47%)

Query: 30  YNGMYVTTNTSSGPLHSDESSSTNWSPNHFRHVINMREAALDFGRKLCGMTENYYYK 86
           + GM ++   ++    +  S+S+   P      I  R A  DF RK+  + ENY Y+
Sbjct: 305 WKGMLLSVTHTTLSTSTSHSTSSGKPPKSAVQAIPQRTAKADFKRKMYVLKENYNYR 361


>AY058623-1|AAL13852.1| 1139|Drosophila melanogaster LD31543p
           protein.
          Length = 1139

 Score = 29.5 bits (63), Expect = 8.6
 Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 3/32 (9%)

Query: 343 QHPNEEWKSHFENRDLIALSAAPLLVHPTHYT 374
           QHPN ++  HFE   L+ L+ + LL  P  YT
Sbjct: 825 QHPNSQFAKHFE---LLKLTESDLLPKPDFYT 853


>AE014298-2841|AAF48954.2| 1139|Drosophila melanogaster CG32533-PA
           protein.
          Length = 1139

 Score = 29.5 bits (63), Expect = 8.6
 Identities = 14/32 (43%), Positives = 19/32 (59%), Gaps = 3/32 (9%)

Query: 343 QHPNEEWKSHFENRDLIALSAAPLLVHPTHYT 374
           QHPN ++  HFE   L+ L+ + LL  P  YT
Sbjct: 825 QHPNSQFAKHFE---LLKLTESDLLPKPDFYT 853


>AE013599-453|AAF59228.1| 3242|Drosophila melanogaster CG2093-PA
            protein.
          Length = 3242

 Score = 29.5 bits (63), Expect = 8.6
 Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 9/57 (15%)

Query: 313  ISERGAKKLLRADPLSKMLPVDEFLPIMFDQHPNEEWKSHFENRDLIALSAAPLLVH 369
            +S+  A K  R D  S  LP+  F    F++H  EE KS ++N     L   PL +H
Sbjct: 2792 VSDEAAAKQFRQDVESIELPLSAF----FEEHSLEEQKSFYDN-----LHLGPLKIH 2839


  Database: fruitfly
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 24,830,863
  Number of sequences in database:  52,641
  
Lambda     K      H
   0.318    0.137    0.419 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,039,689
Number of Sequences: 52641
Number of extensions: 1017469
Number of successful extensions: 2369
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 2358
Number of HSP's gapped (non-prelim): 14
length of query: 402
length of database: 24,830,863
effective HSP length: 87
effective length of query: 315
effective length of database: 20,251,096
effective search space: 6379095240
effective search space used: 6379095240
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 63 (29.5 bits)

- SilkBase 1999-2023 -