BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000599-TA|BGIBMGA000599-PA|IPR011009|Protein kinase-like, IPR000719|Protein kinase (617 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizo... 96 1e-20 SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual 87 7e-18 SPBC119.08 |pmk1|spm1|MAP kinase Pmk1 |Schizosaccharomyces pombe... 37 0.009 SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 33 0.088 SPAC24B11.06c |sty1|spc1, phh1|MAP kinase Sty1|Schizosaccharomyc... 33 0.088 SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 33 0.088 SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|ch... 33 0.15 SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|... 31 0.36 SPBC8D2.01 |gsk31||serine/threonine protein kinase Gsk31|Schizos... 30 1.1 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 29 1.4 SPCC1020.10 |oca2||serine/threonine protein kinase Oca2 |Schizos... 29 1.9 SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1 |Schizosacch... 29 1.9 SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 29 2.5 SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo... 28 3.3 SPCC4G3.08 |psk1||serine/threonine protein kinase Psk1|Schizosac... 27 5.8 SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizos... 27 5.8 SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizo... 27 7.7 SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosa... 27 7.7 SPBC776.08c |||Nrap|Schizosaccharomyces pombe|chr 2|||Manual 27 7.7 SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schiz... 27 7.7 >SPBC336.14c |ppk26||serine/threonine protein kinase Ppk26|Schizosaccharomyces pombe|chr 2|||Manual Length = 589 Score = 95.9 bits (228), Expect = 1e-20 Identities = 60/256 (23%), Positives = 114/256 (44%), Gaps = 2/256 (0%) Query: 258 LPEAVLWSLLVQLTAGLRAIHTAGLACRSLEPNKVIMNGC-RVRIAWCGAADALHSNTND 316 +PE LW+ QLT L +H +G AC L P++++++ R+RI+ C + + SN Sbjct: 330 IPERELWNYFFQLTIALSYLHKSGFACNKLTPSRILVDQTERIRISGCADYELVVSNKPP 389 Query: 317 VVQAQQDDXXXXXXXXXXXXCRTIHCDNLAASMELVARTYSADLKNLILYLLSSSPARRS 376 + + ++ D D ++++ + TYS + +LY +S P ++ Sbjct: 390 LEERKKQDFVDLGVVIANLATGRTDMD-MSSAARAIYSTYSREFYKAVLYFVSEVPEDKN 448 Query: 377 VIDLMPMIGARFYTQVEALERRADAFEDQLSQEIDNXXXXXXXXXXXXVNERPELNLDPA 436 + + F+ + + + E ++S + + + + + Sbjct: 449 LELFLQNHIESFFPIMSSPYVECEKMERKISDAFQHGRFFNILCKIMFIIDNNRASREYP 508 Query: 437 WSETGDRYMLKLFRDYLFHSVTTDGRPWLDQAHLTHCLNQLDGGTAAKVELMSRDEQSVL 496 + + ++ L RDYLFH + D P +D + + L +LD G + L+SRDE + Sbjct: 509 IAREKEISLIYLLRDYLFHQIDEDECPVIDLYQVLNRLGKLDAGINQAIALISRDELDCV 568 Query: 497 VVSYAELKHCLDQAFE 512 VSY ELK LD +E Sbjct: 569 SVSYGELKAWLDNVYE 584 Score = 33.5 bits (73), Expect = 0.088 Identities = 22/85 (25%), Positives = 38/85 (44%), Gaps = 7/85 (8%) Query: 59 IPDSVDMYAELVPLEGAVTHSM------STSYRATNRQNGDYVALRRLHSYT-SPASKRL 111 +P + Y L PL + S +Y+ T+ +G+ L+RL + + + + Sbjct: 210 LPSIISSYTSLAPLNTKLYRYKEQIGFSSWTYKCTSSIDGNAYVLKRLQDCSINIDTSTV 269 Query: 112 EMWKQIDHPNIVRLEEYFSTKAFND 136 + K + HPNIV F T F+D Sbjct: 270 DKLKNVFHPNIVPFHSAFHTDTFHD 294 >SPAC1B1.04c |||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 604 Score = 87.0 bits (206), Expect = 7e-18 Identities = 70/263 (26%), Positives = 120/263 (45%), Gaps = 24/263 (9%) Query: 262 VLWSLLVQLTAGLRAIHTAGLACRSLEPNKVIMNG-CRVRIAWCGAADALHS-NTNDVVQ 319 VLW QL + L +IH++GLA + + V+M G R+ I G D + +T + Sbjct: 337 VLWCFASQLISALYSIHSSGLAAKMVSLKNVLMVGKMRLAIFGLGIMDVIQEESTEPLTS 396 Query: 320 AQQDDXXXXXXXXXXXXCRTIHCDNLAASMELVAR----TYSADLKNLILYLLSSSPARR 375 Q++D T + A L + A L LI L+ + R Sbjct: 397 LQRNDCRDVGLILLALATDTENVTLSTAKAHLTRLKTIVSTDASLVELIEVLIFNEELR- 455 Query: 376 SVIDLMPMIGARFYTQVEALERRADAFEDQLSQEIDNXXXXXXXXXXXXVNERPELNLDP 435 + L+P + + E++ D +E L+++++N +++RPE DP Sbjct: 456 -IQTLLPTMLSYMVNNYESVLLMEDVYETYLAEQVENDRLLRLLLKLEFLDDRPEYVDDP 514 Query: 436 AWSETGDRYMLKLFRDYLF--------------HSVTTDGRPWLDQAHLTHCLNQLDGGT 481 WS +G ++++LFR Y+F S TT R L++AHL CLN+LD GT Sbjct: 515 DWSASGVYFVIRLFRKYMFQVQTIDDASKKPTLQSTTTPPRKLLNKAHLLSCLNKLDAGT 574 Query: 482 AAKVELMSRDEQSVLVVSYAELK 504 ++ L DE + +++S+ E++ Sbjct: 575 DEQILL--EDEFTRIIMSFKEVR 595 Score = 41.1 bits (92), Expect = 4e-04 Identities = 27/107 (25%), Positives = 47/107 (43%), Gaps = 11/107 (10%) Query: 48 YLQPDLNQF---PDIPDSVDMYAELVPLEGAVT-----HSMSTSYRATNRQNG---DYVA 96 YL + QF P +P V Y L+P VT ++ Y+ + NG +V Sbjct: 174 YLTQEFYQFANIPKLPSHVLSYHSLIPRRMIVTVLPVLRYATSIYKVIDGNNGLPYSFVQ 233 Query: 97 LRRLHSYTSPASKRLEMWKQIDHPNIVRLEEYFSTKAFNDICRMNCV 143 LR + W ++D P+++++ E F+T AF C ++ + Sbjct: 234 LRDFTLLNDRNITNVSPWTKVDSPHVIKIREAFTTHAFEQKCMLSFI 280 >SPBC119.08 |pmk1|spm1|MAP kinase Pmk1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 422 Score = 36.7 bits (81), Expect = 0.009 Identities = 23/78 (29%), Positives = 37/78 (47%), Gaps = 2/78 (2%) Query: 241 YTHQKNAMLRAVA-CGALLPEAVLWSLLVQLTAGLRAIHTAGLACRSLEPNKVIMNG-CR 298 Y A L A+ G L +A S + Q+ GL+ IH+A + R L+P +++N C Sbjct: 103 YEELMEADLNAIIKSGQPLTDAHFQSFIYQILCGLKYIHSANVIHRDLKPGNLLVNADCE 162 Query: 299 VRIAWCGAADALHSNTND 316 ++I G A N + Sbjct: 163 LKICDFGLARGCSENPEE 180 >SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 781 Score = 33.5 bits (73), Expect = 0.088 Identities = 17/57 (29%), Positives = 32/57 (56%), Gaps = 1/57 (1%) Query: 266 LLVQLTAGLRAIHTAGLACRSLEPNKVIMNGCRV-RIAWCGAADALHSNTNDVVQAQ 321 ++ Q+ GL IH+ G+A R ++P ++++ V RI GA D L + +D+ + Sbjct: 564 MIKQILRGLNYIHSQGIAHRDIKPENILISEYGVLRITDFGACDVLCNPGDDITAVE 620 >SPAC24B11.06c |sty1|spc1, phh1|MAP kinase Sty1|Schizosaccharomyces pombe|chr 1|||Manual Length = 349 Score = 33.5 bits (73), Expect = 0.088 Identities = 15/42 (35%), Positives = 27/42 (64%), Gaps = 1/42 (2%) Query: 267 LVQLTAGLRAIHTAGLACRSLEPNKVIMN-GCRVRIAWCGAA 307 L Q+ GL+ +H+AG+ R L+P+ +++N C ++I G A Sbjct: 122 LYQILRGLKFVHSAGVIHRDLKPSNILINENCDLKICDFGLA 163 >SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc7|Schizosaccharomyces pombe|chr 2|||Manual Length = 1062 Score = 33.5 bits (73), Expect = 0.088 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 6/54 (11%) Query: 84 YRATNRQNGDYVALRR------LHSYTSPASKRLEMWKQIDHPNIVRLEEYFST 131 YR N +NG+ VA+++ L S S +++ K +DHPNIV+ + T Sbjct: 24 YRGLNIKNGETVAVKKVKLSKMLKSDLSVIKMEIDLLKNLDHPNIVKYRGSYQT 77 >SPAC31G5.09c |spk1||MAP kinase Spk1|Schizosaccharomyces pombe|chr 1|||Manual Length = 372 Score = 32.7 bits (71), Expect = 0.15 Identities = 14/40 (35%), Positives = 27/40 (67%), Gaps = 1/40 (2%) Query: 269 QLTAGLRAIHTAGLACRSLEPNKVIMN-GCRVRIAWCGAA 307 Q+ L+A+H+AG+ R L+P+ +++N C +++A G A Sbjct: 146 QILRALKAMHSAGVVHRDLKPSNLLLNANCDLKVADFGLA 185 >SPCC16C4.11 |pef1||Pho85/PhoA-like cyclin-dependent kinase Pef1|Schizosaccharomyces pombe|chr 3|||Manual Length = 288 Score = 31.5 bits (68), Expect = 0.36 Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 5/54 (9%) Query: 84 YRATNRQNGDYVALRRLH-----SYTSPASKRLEMWKQIDHPNIVRLEEYFSTK 132 Y+ NR G+ VAL+ + S A + + + K++ HPNI+ L + T+ Sbjct: 18 YKGQNRVTGEIVALKVIRIDADEGTPSTAIREISLMKELRHPNIMSLSDVLQTE 71 >SPBC8D2.01 |gsk31||serine/threonine protein kinase Gsk31|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 29.9 bits (64), Expect = 1.1 Identities = 11/38 (28%), Positives = 24/38 (63%) Query: 94 YVALRRLHSYTSPASKRLEMWKQIDHPNIVRLEEYFST 131 ++A++R+ ++ L++ + I HPNI++L +F T Sbjct: 50 FIAVKRVLQDKRYKNRELQIMRAISHPNIIKLIAFFHT 87 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 29.5 bits (63), Expect = 1.4 Identities = 19/53 (35%), Positives = 28/53 (52%), Gaps = 8/53 (15%) Query: 79 SMSTSYRATNRQNGDYVALRR--LHS--YTSPASKRLE----MWKQIDHPNIV 123 S T YRA N GD +A++ LH + P KR++ + + DHPN+V Sbjct: 1130 SFGTVYRAVNLDTGDLMAVKEVALHKPRISRPMIKRIKGEMLVLELFDHPNVV 1182 >SPCC1020.10 |oca2||serine/threonine protein kinase Oca2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 650 Score = 29.1 bits (62), Expect = 1.9 Identities = 21/62 (33%), Positives = 34/62 (54%), Gaps = 4/62 (6%) Query: 248 MLRAVACGAL-LPEAVLWSLLVQLTAGLRAIHTAGLACRSLE-PNKVIMNGCRVRIAWCG 305 M V G + +PE ++ QL +G+ +H+ GLA R L+ N V+ + C V+I G Sbjct: 388 MFSIVMSGKMTMPE--VYCCFKQLLSGVAYLHSMGLAHRDLKLDNLVVDSNCFVKIIDFG 445 Query: 306 AA 307 +A Sbjct: 446 SA 447 >SPAC2F3.15 |lsk1||latrunculin sensitive kinase Lsk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 593 Score = 29.1 bits (62), Expect = 1.9 Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 6/56 (10%) Query: 84 YRATNRQNGDYVALRRLH------SYTSPASKRLEMWKQIDHPNIVRLEEYFSTKA 133 Y+A N GD VAL+R+ + + +++ +++ H NIVRL E K+ Sbjct: 292 YKAINTVTGDLVALKRIRLEQEKDGFPITTVREVKILQRLRHKNIVRLLEIMVEKS 347 >SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1576 Score = 28.7 bits (61), Expect = 2.5 Identities = 11/38 (28%), Positives = 20/38 (52%) Query: 258 LPEAVLWSLLVQLTAGLRAIHTAGLACRSLEPNKVIMN 295 +P +W L Q+ L IH+ G+ R L+P + ++ Sbjct: 744 IPVDEMWRLFRQILEALAYIHSRGMMHRDLKPGNIFLD 781 >SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1401 Score = 28.3 bits (60), Expect = 3.3 Identities = 15/52 (28%), Positives = 28/52 (53%) Query: 244 QKNAMLRAVACGALLPEAVLWSLLVQLTAGLRAIHTAGLACRSLEPNKVIMN 295 Q ++ +A G + E VL +VQL GL IH+ + R ++P ++++ Sbjct: 1119 QGGSLADLLAHGRIEDENVLKVYVVQLLEGLAYIHSQHILHRDIKPANILLD 1170 >SPCC4G3.08 |psk1||serine/threonine protein kinase Psk1|Schizosaccharomyces pombe|chr 3|||Manual Length = 436 Score = 27.5 bits (58), Expect = 5.8 Identities = 12/39 (30%), Positives = 21/39 (53%) Query: 257 LLPEAVLWSLLVQLTAGLRAIHTAGLACRSLEPNKVIMN 295 +LPE V+ +LT L +H G+ R L+P +++ Sbjct: 186 MLPEDVVAFYTAELTLALIHLHKLGIVYRDLKPENCLLD 224 >SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 27.5 bits (58), Expect = 5.8 Identities = 15/46 (32%), Positives = 21/46 (45%) Query: 57 PDIPDSVDMYAELVPLEGAVTHSMSTSYRATNRQNGDYVALRRLHS 102 PD+P + M P+ +S YR N Q D +AL +HS Sbjct: 485 PDVPQKLAMAQGWYPVVQKSLIILSKIYRLVNSQVFDEIALELVHS 530 >SPCC1322.08 |srk1|mkp1|MAPK-activated protein kinase Srk1|Schizosaccharomyces pombe|chr 3|||Manual Length = 580 Score = 27.1 bits (57), Expect = 7.7 Identities = 8/26 (30%), Positives = 18/26 (69%) Query: 109 KRLEMWKQIDHPNIVRLEEYFSTKAF 134 K +++ +++ HPNI++L E+ T + Sbjct: 180 KEVQIMRRVKHPNIIQLLEFIQTPEY 205 >SPAC167.01 |ppk4||serine/threonine protein kinase Ppk4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1072 Score = 27.1 bits (57), Expect = 7.7 Identities = 13/45 (28%), Positives = 23/45 (51%) Query: 250 RAVACGALLPEAVLWSLLVQLTAGLRAIHTAGLACRSLEPNKVIM 294 + +A L L SLL Q+ G+ +H+ L R L+P +++ Sbjct: 738 KPIAYDDLFKSIDLVSLLYQIAFGVSHLHSLDLVHRDLKPQNILL 782 >SPBC776.08c |||Nrap|Schizosaccharomyces pombe|chr 2|||Manual Length = 1097 Score = 27.1 bits (57), Expect = 7.7 Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 3/47 (6%) Query: 20 STNKQGLAATFYNPETIRSEIYDRNDDVYLQPD---LNQFPDIPDSV 63 S L A+F P+T+ I+ ++ +L PD + F +IP+S+ Sbjct: 127 SAKDTNLRASFVPPKTVTPGIFSCSNKFFLNPDGWSYDLFLEIPESI 173 >SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schizosaccharomyces pombe|chr 2|||Manual Length = 526 Score = 27.1 bits (57), Expect = 7.7 Identities = 11/39 (28%), Positives = 20/39 (51%) Query: 257 LLPEAVLWSLLVQLTAGLRAIHTAGLACRSLEPNKVIMN 295 +LPE ++TA L +H G R L+P ++++ Sbjct: 251 ILPEKDACFYAAEVTAALEYLHLMGFIYRDLKPENILLH 289 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.320 0.135 0.412 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,709,964 Number of Sequences: 5004 Number of extensions: 108321 Number of successful extensions: 264 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 240 Number of HSP's gapped (non-prelim): 25 length of query: 617 length of database: 2,362,478 effective HSP length: 77 effective length of query: 540 effective length of database: 1,977,170 effective search space: 1067671800 effective search space used: 1067671800 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.8 bits) S2: 57 (27.1 bits)
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