SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA000596-TA|BGIBMGA000596-PA|IPR004908|ATPase, V1 complex,
subunit H
         (478 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces p...   219   9e-58
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ...    37   0.007
SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pom...    33   0.088
SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc...    29   1.1  
SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog Taz1|Schizosacc...    28   3.3  
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi...    27   4.4  
SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces pomb...    27   4.4  
SPBC24C6.07 |cdc14||SIN component Cdc14|Schizosaccharomyces pomb...    27   4.4  
SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protei...    27   5.8  
SPAC513.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||...    27   5.8  
SPAC1952.06c |||DUF1716 family protein|Schizosaccharomyces pombe...    27   7.6  
SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe...    27   7.6  
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc...    27   7.6  
SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac...    27   7.6  

>SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 450

 Score =  219 bits (534), Expect = 9e-58
 Identities = 134/419 (31%), Positives = 224/419 (53%), Gaps = 14/419 (3%)

Query: 38  SEIRQTRINWQSYLQSQMITQRDHDFIVNLDQR---GQKDLPDKNPDACAEVFLNLLTHI 94
           + +R   I WQ Y +S  + + +   I NL  +         +++  A + +FL LL+  
Sbjct: 29  NNVRCVAIPWQGYQRSGSLEENELQEIENLTGKPLSAYVKTAEEDTTAYSNLFLKLLSMK 88

Query: 95  SKDHTIQYILVLIDDILSEDKSRVKIFRETKFSGNVWQPFLNLLNRQDEFVQHMTARIIA 154
                + + LV + D L      +  F    +  +  +   + +N  D+  + + AR+ A
Sbjct: 89  DTPDVVNFALVKLADTLLNSNKFLSAFGPAFY--DFLEKDESYINYLDDDSKLLFARVFA 146

Query: 155 KLACWHPQLMDKSDLHFYLSWLKDQLKTNNNDYIQSVARCLQMMLRIDEYRFAFLSVDGI 214
             +   P  + K+    +L +L   +++ N        +CL  +L +  +R+A  + +  
Sbjct: 147 LCSSSSPCSVAKA-FTLFLEYLGKLMQSLNPLTRLFAVQCLNGVLTLKAHRYALWAENTC 205

Query: 215 S-TLLSILASRV-NFQVQYQLVFCLWVLTFNPLLAEKMNK-FNVIPILADILSDSVKEKV 271
           S  L  +L + + + Q+QY  +FC W LTF   +A+ +NK F++I +L  I+    K KV
Sbjct: 206 SFRLAELLRNSIGDTQLQYYSLFCFWQLTFESHIAQDINKRFDLIKLLVQIIRSDTKTKV 265

Query: 272 TRIVLAVFRNLIEKPEDQQVAKEHCIAMVQCKVLKQLSILEQKRSDDEDIMNDVEYLNER 331
            R+VLA+  NLI+K       K+    M+   V K + +L++++  DEDI N ++++   
Sbjct: 266 YRLVLAILVNLIDK-----APKDTISTMLLEHVDKAVQLLQKRKWADEDITNYLDFITST 320

Query: 332 LQASVQDLSSFDQYATEVKSGRLEWSPVHKSAKFWRENAARLNERGQELLRTLVHLLEKS 391
           L  S + LS+FD Y +E+ +G L WSP H+S  FW +NA RLNE    LL+ L H+++ +
Sbjct: 321 LDESSKHLSTFDMYKSELDTGILHWSPSHRSEDFWHQNAKRLNEDNYALLKKLFHIVQYN 380

Query: 392 RDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLLSHDDPNVRYEALLAVQKLM 450
            D   LAVAC+D+G Y+R YP G+ +I + G KQR+M L+SH DP VR+EAL  VQ LM
Sbjct: 381 EDNTSLAVACHDLGAYIRSYPEGRSLIIKYGAKQRIMDLMSHPDPEVRFEALSTVQLLM 439


>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1044

 Score = 36.7 bits (81), Expect = 0.007
 Identities = 38/144 (26%), Positives = 68/144 (47%), Gaps = 16/144 (11%)

Query: 245 LLAEKMNKFNVIPILADILSDSVKEKVTRIVLAVFRNLIEKPEDQQVAKEHCIAMVQCKV 304
           LL EK+NK N     AD  ++S K  +    L+      +   D    KE+ I+ ++ K+
Sbjct: 761 LLTEKLNKKN-----AD--TESFKNTIREAELSK-----KALNDNLGNKENIISDLKNKL 808

Query: 305 LKQLSILEQKRSDDEDIMNDVEYLNERLQASVQDLSSFD----QYATEVKSGRLEWSPVH 360
            ++ + L++ +S      N +E LNER+ A+  +LSS +      A E+K  + + S + 
Sbjct: 809 SEESTRLQELQSQLNQDKNQIETLNERISAAADELSSMESINKNQANELKLAKQKCSNLQ 868

Query: 361 KSAKFWRENAARLNERGQELLRTL 384
           +   F  + A    E+   L + L
Sbjct: 869 EKINFGNKLAKEHTEKISSLEKDL 892


>SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 550

 Score = 33.1 bits (72), Expect = 0.088
 Identities = 40/189 (21%), Positives = 80/189 (42%), Gaps = 8/189 (4%)

Query: 122 RETKFSGNVWQPFLNLLNRQDEFVQHMTARIIAKLA--CWHPQLMDKSDLHFYLSWLKDQ 179
           R+   S       ++LL   D  VQ+     I+ +A    H + + +S+     S +  Q
Sbjct: 201 RQQLVSAGTIPVLVSLLPSSDTDVQYYCTTSISNIAVDAVHRKRLAQSEPKLVRSLI--Q 258

Query: 180 LKTNNNDYIQSVARCLQMMLRIDE-YRFAFLSVDGISTLLSILASRVNFQVQYQLVFCLW 238
           L   ++  +Q  A      L  DE Y+   +  + + +LL +L S     +    V C+ 
Sbjct: 259 LMDTSSPKVQCQAALALRNLASDERYQIEIVQSNALPSLLRLLRSSY-LPLILASVACIR 317

Query: 239 VLTFNPLLAEKMNKFNVIPILADILSDSVKEKVTRIVLAVFRNLIEKPE--DQQVAKEHC 296
            ++ +PL    +     +  L D+LS +  E++    ++  RNL    E   + + + + 
Sbjct: 318 NISIHPLNESPIIDAGFLRPLVDLLSCTENEEIQCHAVSTLRNLAASSERNKRAIIEANA 377

Query: 297 IAMVQCKVL 305
           I  ++C +L
Sbjct: 378 IQKLRCLIL 386


>SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1233

 Score = 29.5 bits (63), Expect = 1.1
 Identities = 19/82 (23%), Positives = 39/82 (47%), Gaps = 1/82 (1%)

Query: 301 QCKVLKQLSILEQKRSDDEDIMNDVEYLNERLQASVQ-DLSSFDQYATEVKSGRLEWSPV 359
           Q +  KQ S+LE + S ++  ++D     ER+   ++ D  S D Y    ++   E +  
Sbjct: 812 QLEFTKQKSLLENRISFEKQRVSDTRLRLERMHKFIEKDQESIDNYEQNREALESEVATA 871

Query: 360 HKSAKFWRENAARLNERGQELL 381
               +  +E+ A  N + +++L
Sbjct: 872 EAELELLKEDFASENSKTEKIL 893


>SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog
           Taz1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 663

 Score = 27.9 bits (59), Expect = 3.3
 Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 1/52 (1%)

Query: 4   HVKRRENQEEK-YNPTKVFVREDMIAATSVLQIRASEIRQTRINWQSYLQSQ 54
           H+++ EN   K + PT++  +  +I A  + Q R  E+    +NW++    Q
Sbjct: 585 HIQKLENGPLKTFGPTQIKDKARLIKARFMKQNRLQELYSKSLNWKNVTVGQ 636


>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 865

 Score = 27.5 bits (58), Expect = 4.4
 Identities = 31/144 (21%), Positives = 63/144 (43%), Gaps = 7/144 (4%)

Query: 120 IFRETKFSGNVWQPFLNLLNRQDEFVQHMTARIIAKLACWHPQLMDK-SDLHFYLSWLKD 178
           I+R  + S + +    N+ N  D F+Q    + ++ L   +P++ DK SDL   +    D
Sbjct: 248 IYRIRQLSTSTYSSEHNIGNISDPFLQVKILQFLSILGQNNPKIYDKMSDLLAQVCTNTD 307

Query: 179 QLKTNNNDYIQSVARCLQMMLRIDEYRFAFLSVDGISTLLSILASRVNFQVQYQLVFCLW 238
             +   N  +    R +     +D    + L V G++ L   L +R N   +Y  +  L 
Sbjct: 308 SSRNAGNAILYQAVRTI-----LDLNSDSSLRVLGVNILAKFLGNRDN-NTRYVALNMLK 361

Query: 239 VLTFNPLLAEKMNKFNVIPILADI 262
           ++  +   A + ++  ++  L D+
Sbjct: 362 LVVNSEENAVQRHRSTILACLNDV 385


>SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 649

 Score = 27.5 bits (58), Expect = 4.4
 Identities = 22/85 (25%), Positives = 45/85 (52%), Gaps = 9/85 (10%)

Query: 281 NLIEKPEDQQVAKEHCIAMVQCKVLKQLSILEQKRSDDEDIMNDV-EYLNERLQASVQDL 339
           N +EK +D+   KE C   V+ ++ KQ+++L +KR  +E++  ++   +N+  + S    
Sbjct: 466 NFVEK-DDRDAIKE-C---VETQLNKQINLLVKKRVTEENLEQEISSIINDLPKISTTKR 520

Query: 340 SSFDQYATEVKSGRL---EWSPVHK 361
             +++   EV    +   E +PV K
Sbjct: 521 KDYEELPEEVSETSINIAEHTPVLK 545


>SPBC24C6.07 |cdc14||SIN component Cdc14|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 240

 Score = 27.5 bits (58), Expect = 4.4
 Identities = 13/53 (24%), Positives = 25/53 (47%)

Query: 381 LRTLVHLLEKSRDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLLSH 433
           LR  +HLL+ S+  ++   A   +   +   P    + EQ+ G Q++  +  H
Sbjct: 118 LRLFIHLLQPSQPSMLQVAAMKTLVCIMADRPLAIRLFEQINGLQQICVVFKH 170


>SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1462

 Score = 27.1 bits (57), Expect = 5.8
 Identities = 31/142 (21%), Positives = 64/142 (45%), Gaps = 8/142 (5%)

Query: 172 YLSWLKDQLKTNNNDYIQSVA-RCLQ---MMLRIDEYRFAFLSVDGISTLLSILASRVNF 227
           Y +WLK ++    N  I  +A R LQ   + + ID + F    ++     LS  +     
Sbjct: 80  YPTWLKFRVPMLKNLVIDHIASRDLQKRVLNILIDLWHFNPSEIEKSLIHLSTTSKSAET 139

Query: 228 QVQYQLVFCLWVLTFNPLLAEKMNKFN-VIPILADILSDSVKEKVTRIVLAVFRNLIEKP 286
           ++Q    F  +VL  N  L+  +      + I  +  + SV+E+   ++L +++NL    
Sbjct: 140 RIQ---CFKWFVLAHNAHLSFDVKSLRPALYINLENANPSVREEAKEVLLLIYKNLSTSA 196

Query: 287 EDQQVAKEHCIAMVQCKVLKQL 308
           + Q +      + ++ ++L+ L
Sbjct: 197 KMQFITDVETTSGLRREILQSL 218


>SPAC513.04 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 100

 Score = 27.1 bits (57), Expect = 5.8
 Identities = 16/94 (17%), Positives = 42/94 (44%), Gaps = 1/94 (1%)

Query: 244 PLLAEKMNKFNVIPILADILSDSVKEKVTRIVLAVFRNLIEKPEDQQVAKEHCIAMVQCK 303
           PL     +  N+I ++   L+  +  K   +++ +F  L++    ++  K+    +VQ +
Sbjct: 8   PLYFRIWSSLNIICLMVTFLNVQLS-KTPIVLMPLFIALLKNNRKRETKKQTISVLVQKR 66

Query: 304 VLKQLSILEQKRSDDEDIMNDVEYLNERLQASVQ 337
             K   +L+   + +   +   +YL ++    +Q
Sbjct: 67  EEKTKLVLDDANNHNHAFLYHCQYLGQKKHVVIQ 100


>SPAC1952.06c |||DUF1716 family protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 564

 Score = 26.6 bits (56), Expect = 7.6
 Identities = 18/70 (25%), Positives = 35/70 (50%), Gaps = 2/70 (2%)

Query: 176 LKDQLKTNNNDYIQSVARCLQMMLRIDEYRFAFLSVDGISTLLSILASRVNFQVQYQLVF 235
           L+D  + +  + +++V  CL  +++  + +  FL  +GI   L IL  +   + +Y  + 
Sbjct: 301 LRDPTQGSEEEMMENVFDCLCSLVQETKGKSLFLKEEGIE--LCILNMKHKGKSRYSTIK 358

Query: 236 CLWVLTFNPL 245
            L  L F PL
Sbjct: 359 VLDYLLFGPL 368


>SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 548

 Score = 26.6 bits (56), Expect = 7.6
 Identities = 16/66 (24%), Positives = 35/66 (53%)

Query: 123 ETKFSGNVWQPFLNLLNRQDEFVQHMTARIIAKLACWHPQLMDKSDLHFYLSWLKDQLKT 182
           E++ S  + +   +LL+ QD+  + ++  ++AKL   HP L+ K      + +L   L +
Sbjct: 58  ESRGSMELLENCFSLLHAQDDTSKFVSLTMLAKLLNDHPNLIFKCWERMDMKFLDRLLLS 117

Query: 183 NNNDYI 188
            + +Y+
Sbjct: 118 THYEYV 123


>SPBC6B1.04 |mde4||monopolin-like complex subunit
           Mde4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 421

 Score = 26.6 bits (56), Expect = 7.6
 Identities = 15/55 (27%), Positives = 28/55 (50%)

Query: 297 IAMVQCKVLKQLSILEQKRSDDEDIMNDVEYLNERLQASVQDLSSFDQYATEVKS 351
           I  +Q + L    +LEQ++++  D    VE L  ++   VQ+++S +      KS
Sbjct: 70  IKFLQNEKLNNEKLLEQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKS 124


>SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit
           Vps23|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 487

 Score = 26.6 bits (56), Expect = 7.6
 Identities = 18/73 (24%), Positives = 32/73 (43%), Gaps = 2/73 (2%)

Query: 385 VHLLEKSRDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLLSHDDPNVR--YEA 442
           ++L   + D  +L  AC  +  Y    P    ++++ GGK +   LL     N R  +  
Sbjct: 65  IYLSTPAFDKTILTSACITVRSYYPSQPPIVQLLDEKGGKHKYTSLLLQLWKNERSVFNI 124

Query: 443 LLAVQKLMVHNWE 455
              VQ L+  ++E
Sbjct: 125 YRLVQALIKQDFE 137


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.322    0.135    0.396 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,149,523
Number of Sequences: 5004
Number of extensions: 89590
Number of successful extensions: 321
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 311
Number of HSP's gapped (non-prelim): 17
length of query: 478
length of database: 2,362,478
effective HSP length: 75
effective length of query: 403
effective length of database: 1,987,178
effective search space: 800832734
effective search space used: 800832734
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 56 (26.6 bits)

- SilkBase 1999-2023 -