BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000596-TA|BGIBMGA000596-PA|IPR004908|ATPase, V1 complex, subunit H (478 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces p... 219 9e-58 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 37 0.007 SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pom... 33 0.088 SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Sc... 29 1.1 SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog Taz1|Schizosacc... 28 3.3 SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 27 4.4 SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces pomb... 27 4.4 SPBC24C6.07 |cdc14||SIN component Cdc14|Schizosaccharomyces pomb... 27 4.4 SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protei... 27 5.8 SPAC513.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 27 5.8 SPAC1952.06c |||DUF1716 family protein|Schizosaccharomyces pombe... 27 7.6 SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe... 27 7.6 SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 27 7.6 SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosac... 27 7.6 >SPAC7D4.10 |vma13||V-type ATPase subunit H|Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 219 bits (534), Expect = 9e-58 Identities = 134/419 (31%), Positives = 224/419 (53%), Gaps = 14/419 (3%) Query: 38 SEIRQTRINWQSYLQSQMITQRDHDFIVNLDQR---GQKDLPDKNPDACAEVFLNLLTHI 94 + +R I WQ Y +S + + + I NL + +++ A + +FL LL+ Sbjct: 29 NNVRCVAIPWQGYQRSGSLEENELQEIENLTGKPLSAYVKTAEEDTTAYSNLFLKLLSMK 88 Query: 95 SKDHTIQYILVLIDDILSEDKSRVKIFRETKFSGNVWQPFLNLLNRQDEFVQHMTARIIA 154 + + LV + D L + F + + + + +N D+ + + AR+ A Sbjct: 89 DTPDVVNFALVKLADTLLNSNKFLSAFGPAFY--DFLEKDESYINYLDDDSKLLFARVFA 146 Query: 155 KLACWHPQLMDKSDLHFYLSWLKDQLKTNNNDYIQSVARCLQMMLRIDEYRFAFLSVDGI 214 + P + K+ +L +L +++ N +CL +L + +R+A + + Sbjct: 147 LCSSSSPCSVAKA-FTLFLEYLGKLMQSLNPLTRLFAVQCLNGVLTLKAHRYALWAENTC 205 Query: 215 S-TLLSILASRV-NFQVQYQLVFCLWVLTFNPLLAEKMNK-FNVIPILADILSDSVKEKV 271 S L +L + + + Q+QY +FC W LTF +A+ +NK F++I +L I+ K KV Sbjct: 206 SFRLAELLRNSIGDTQLQYYSLFCFWQLTFESHIAQDINKRFDLIKLLVQIIRSDTKTKV 265 Query: 272 TRIVLAVFRNLIEKPEDQQVAKEHCIAMVQCKVLKQLSILEQKRSDDEDIMNDVEYLNER 331 R+VLA+ NLI+K K+ M+ V K + +L++++ DEDI N ++++ Sbjct: 266 YRLVLAILVNLIDK-----APKDTISTMLLEHVDKAVQLLQKRKWADEDITNYLDFITST 320 Query: 332 LQASVQDLSSFDQYATEVKSGRLEWSPVHKSAKFWRENAARLNERGQELLRTLVHLLEKS 391 L S + LS+FD Y +E+ +G L WSP H+S FW +NA RLNE LL+ L H+++ + Sbjct: 321 LDESSKHLSTFDMYKSELDTGILHWSPSHRSEDFWHQNAKRLNEDNYALLKKLFHIVQYN 380 Query: 392 RDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLLSHDDPNVRYEALLAVQKLM 450 D LAVAC+D+G Y+R YP G+ +I + G KQR+M L+SH DP VR+EAL VQ LM Sbjct: 381 EDNTSLAVACHDLGAYIRSYPEGRSLIIKYGAKQRIMDLMSHPDPEVRFEALSTVQLLM 439 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 36.7 bits (81), Expect = 0.007 Identities = 38/144 (26%), Positives = 68/144 (47%), Gaps = 16/144 (11%) Query: 245 LLAEKMNKFNVIPILADILSDSVKEKVTRIVLAVFRNLIEKPEDQQVAKEHCIAMVQCKV 304 LL EK+NK N AD ++S K + L+ + D KE+ I+ ++ K+ Sbjct: 761 LLTEKLNKKN-----AD--TESFKNTIREAELSK-----KALNDNLGNKENIISDLKNKL 808 Query: 305 LKQLSILEQKRSDDEDIMNDVEYLNERLQASVQDLSSFD----QYATEVKSGRLEWSPVH 360 ++ + L++ +S N +E LNER+ A+ +LSS + A E+K + + S + Sbjct: 809 SEESTRLQELQSQLNQDKNQIETLNERISAAADELSSMESINKNQANELKLAKQKCSNLQ 868 Query: 361 KSAKFWRENAARLNERGQELLRTL 384 + F + A E+ L + L Sbjct: 869 EKINFGNKLAKEHTEKISSLEKDL 892 >SPBC354.14c |vac8||vacuolar protein Vac8|Schizosaccharomyces pombe|chr 2|||Manual Length = 550 Score = 33.1 bits (72), Expect = 0.088 Identities = 40/189 (21%), Positives = 80/189 (42%), Gaps = 8/189 (4%) Query: 122 RETKFSGNVWQPFLNLLNRQDEFVQHMTARIIAKLA--CWHPQLMDKSDLHFYLSWLKDQ 179 R+ S ++LL D VQ+ I+ +A H + + +S+ S + Q Sbjct: 201 RQQLVSAGTIPVLVSLLPSSDTDVQYYCTTSISNIAVDAVHRKRLAQSEPKLVRSLI--Q 258 Query: 180 LKTNNNDYIQSVARCLQMMLRIDE-YRFAFLSVDGISTLLSILASRVNFQVQYQLVFCLW 238 L ++ +Q A L DE Y+ + + + +LL +L S + V C+ Sbjct: 259 LMDTSSPKVQCQAALALRNLASDERYQIEIVQSNALPSLLRLLRSSY-LPLILASVACIR 317 Query: 239 VLTFNPLLAEKMNKFNVIPILADILSDSVKEKVTRIVLAVFRNLIEKPE--DQQVAKEHC 296 ++ +PL + + L D+LS + E++ ++ RNL E + + + + Sbjct: 318 NISIHPLNESPIIDAGFLRPLVDLLSCTENEEIQCHAVSTLRNLAASSERNKRAIIEANA 377 Query: 297 IAMVQCKVL 305 I ++C +L Sbjct: 378 IQKLRCLIL 386 >SPBC29A10.04 |psm1|smc1|mitotic cohesin complex subunit Psm1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1233 Score = 29.5 bits (63), Expect = 1.1 Identities = 19/82 (23%), Positives = 39/82 (47%), Gaps = 1/82 (1%) Query: 301 QCKVLKQLSILEQKRSDDEDIMNDVEYLNERLQASVQ-DLSSFDQYATEVKSGRLEWSPV 359 Q + KQ S+LE + S ++ ++D ER+ ++ D S D Y ++ E + Sbjct: 812 QLEFTKQKSLLENRISFEKQRVSDTRLRLERMHKFIEKDQESIDNYEQNREALESEVATA 871 Query: 360 HKSAKFWRENAARLNERGQELL 381 + +E+ A N + +++L Sbjct: 872 EAELELLKEDFASENSKTEKIL 893 >SPAC16A10.07c |taz1|myb1, myb|human TRF ortholog Taz1|Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 27.9 bits (59), Expect = 3.3 Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 1/52 (1%) Query: 4 HVKRRENQEEK-YNPTKVFVREDMIAATSVLQIRASEIRQTRINWQSYLQSQ 54 H+++ EN K + PT++ + +I A + Q R E+ +NW++ Q Sbjct: 585 HIQKLENGPLKTFGPTQIKDKARLIKARFMKQNRLQELYSKSLNWKNVTVGQ 636 >SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schizosaccharomyces pombe|chr 3|||Manual Length = 865 Score = 27.5 bits (58), Expect = 4.4 Identities = 31/144 (21%), Positives = 63/144 (43%), Gaps = 7/144 (4%) Query: 120 IFRETKFSGNVWQPFLNLLNRQDEFVQHMTARIIAKLACWHPQLMDK-SDLHFYLSWLKD 178 I+R + S + + N+ N D F+Q + ++ L +P++ DK SDL + D Sbjct: 248 IYRIRQLSTSTYSSEHNIGNISDPFLQVKILQFLSILGQNNPKIYDKMSDLLAQVCTNTD 307 Query: 179 QLKTNNNDYIQSVARCLQMMLRIDEYRFAFLSVDGISTLLSILASRVNFQVQYQLVFCLW 238 + N + R + +D + L V G++ L L +R N +Y + L Sbjct: 308 SSRNAGNAILYQAVRTI-----LDLNSDSSLRVLGVNILAKFLGNRDN-NTRYVALNMLK 361 Query: 239 VLTFNPLLAEKMNKFNVIPILADI 262 ++ + A + ++ ++ L D+ Sbjct: 362 LVVNSEENAVQRHRSTILACLNDV 385 >SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces pombe|chr 1|||Manual Length = 649 Score = 27.5 bits (58), Expect = 4.4 Identities = 22/85 (25%), Positives = 45/85 (52%), Gaps = 9/85 (10%) Query: 281 NLIEKPEDQQVAKEHCIAMVQCKVLKQLSILEQKRSDDEDIMNDV-EYLNERLQASVQDL 339 N +EK +D+ KE C V+ ++ KQ+++L +KR +E++ ++ +N+ + S Sbjct: 466 NFVEK-DDRDAIKE-C---VETQLNKQINLLVKKRVTEENLEQEISSIINDLPKISTTKR 520 Query: 340 SSFDQYATEVKSGRL---EWSPVHK 361 +++ EV + E +PV K Sbjct: 521 KDYEELPEEVSETSINIAEHTPVLK 545 >SPBC24C6.07 |cdc14||SIN component Cdc14|Schizosaccharomyces pombe|chr 2|||Manual Length = 240 Score = 27.5 bits (58), Expect = 4.4 Identities = 13/53 (24%), Positives = 25/53 (47%) Query: 381 LRTLVHLLEKSRDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLLSH 433 LR +HLL+ S+ ++ A + + P + EQ+ G Q++ + H Sbjct: 118 LRLFIHLLQPSQPSMLQVAAMKTLVCIMADRPLAIRLFEQINGLQQICVVFKH 170 >SPAC3G9.12 |peg1|cls1|CLASP family microtubule-associated protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1462 Score = 27.1 bits (57), Expect = 5.8 Identities = 31/142 (21%), Positives = 64/142 (45%), Gaps = 8/142 (5%) Query: 172 YLSWLKDQLKTNNNDYIQSVA-RCLQ---MMLRIDEYRFAFLSVDGISTLLSILASRVNF 227 Y +WLK ++ N I +A R LQ + + ID + F ++ LS + Sbjct: 80 YPTWLKFRVPMLKNLVIDHIASRDLQKRVLNILIDLWHFNPSEIEKSLIHLSTTSKSAET 139 Query: 228 QVQYQLVFCLWVLTFNPLLAEKMNKFN-VIPILADILSDSVKEKVTRIVLAVFRNLIEKP 286 ++Q F +VL N L+ + + I + + SV+E+ ++L +++NL Sbjct: 140 RIQ---CFKWFVLAHNAHLSFDVKSLRPALYINLENANPSVREEAKEVLLLIYKNLSTSA 196 Query: 287 EDQQVAKEHCIAMVQCKVLKQL 308 + Q + + ++ ++L+ L Sbjct: 197 KMQFITDVETTSGLRREILQSL 218 >SPAC513.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 100 Score = 27.1 bits (57), Expect = 5.8 Identities = 16/94 (17%), Positives = 42/94 (44%), Gaps = 1/94 (1%) Query: 244 PLLAEKMNKFNVIPILADILSDSVKEKVTRIVLAVFRNLIEKPEDQQVAKEHCIAMVQCK 303 PL + N+I ++ L+ + K +++ +F L++ ++ K+ +VQ + Sbjct: 8 PLYFRIWSSLNIICLMVTFLNVQLS-KTPIVLMPLFIALLKNNRKRETKKQTISVLVQKR 66 Query: 304 VLKQLSILEQKRSDDEDIMNDVEYLNERLQASVQ 337 K +L+ + + + +YL ++ +Q Sbjct: 67 EEKTKLVLDDANNHNHAFLYHCQYLGQKKHVVIQ 100 >SPAC1952.06c |||DUF1716 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 564 Score = 26.6 bits (56), Expect = 7.6 Identities = 18/70 (25%), Positives = 35/70 (50%), Gaps = 2/70 (2%) Query: 176 LKDQLKTNNNDYIQSVARCLQMMLRIDEYRFAFLSVDGISTLLSILASRVNFQVQYQLVF 235 L+D + + + +++V CL +++ + + FL +GI L IL + + +Y + Sbjct: 301 LRDPTQGSEEEMMENVFDCLCSLVQETKGKSLFLKEEGIE--LCILNMKHKGKSRYSTIK 358 Query: 236 CLWVLTFNPL 245 L L F PL Sbjct: 359 VLDYLLFGPL 368 >SPCC16C4.02c |||DUF1941 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 548 Score = 26.6 bits (56), Expect = 7.6 Identities = 16/66 (24%), Positives = 35/66 (53%) Query: 123 ETKFSGNVWQPFLNLLNRQDEFVQHMTARIIAKLACWHPQLMDKSDLHFYLSWLKDQLKT 182 E++ S + + +LL+ QD+ + ++ ++AKL HP L+ K + +L L + Sbjct: 58 ESRGSMELLENCFSLLHAQDDTSKFVSLTMLAKLLNDHPNLIFKCWERMDMKFLDRLLLS 117 Query: 183 NNNDYI 188 + +Y+ Sbjct: 118 THYEYV 123 >SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosaccharomyces pombe|chr 2|||Manual Length = 421 Score = 26.6 bits (56), Expect = 7.6 Identities = 15/55 (27%), Positives = 28/55 (50%) Query: 297 IAMVQCKVLKQLSILEQKRSDDEDIMNDVEYLNERLQASVQDLSSFDQYATEVKS 351 I +Q + L +LEQ++++ D VE L ++ VQ+++S + KS Sbjct: 70 IKFLQNEKLNNEKLLEQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKS 124 >SPAC11H11.01 |sst6|cps23|ESCRT I complex subunit Vps23|Schizosaccharomyces pombe|chr 1|||Manual Length = 487 Score = 26.6 bits (56), Expect = 7.6 Identities = 18/73 (24%), Positives = 32/73 (43%), Gaps = 2/73 (2%) Query: 385 VHLLEKSRDPVVLAVACYDIGEYVRHYPRGKHIIEQLGGKQRVMYLLSHDDPNVR--YEA 442 ++L + D +L AC + Y P ++++ GGK + LL N R + Sbjct: 65 IYLSTPAFDKTILTSACITVRSYYPSQPPIVQLLDEKGGKHKYTSLLLQLWKNERSVFNI 124 Query: 443 LLAVQKLMVHNWE 455 VQ L+ ++E Sbjct: 125 YRLVQALIKQDFE 137 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.322 0.135 0.396 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,149,523 Number of Sequences: 5004 Number of extensions: 89590 Number of successful extensions: 321 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 311 Number of HSP's gapped (non-prelim): 17 length of query: 478 length of database: 2,362,478 effective HSP length: 75 effective length of query: 403 effective length of database: 1,987,178 effective search space: 800832734 effective search space used: 800832734 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.9 bits) S2: 56 (26.6 bits)
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