BLASTP 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= BGIBMGA000589-TA|BGIBMGA000589-PA|IPR000727|Target SNARE coiled-coil region (104 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 27 0.60 SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schi... 27 0.80 SPAPB1A10.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 0.80 SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 26 1.4 SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pom... 25 1.8 SPCC569.01c |||DUF1773 family protein 5|Schizosaccharomyces pomb... 25 1.8 SPAC14C4.09 |agn1||glucan endo-1,3-alpha-glucosidase Agn1|Schizo... 25 1.8 SPCP20C8.01c |||B13958 domain|Schizosaccharomyces pombe|chr 3|||... 25 1.8 SPBC36B7.07 |tlg1||SNARE Tgl1|Schizosaccharomyces pombe|chr 2|||... 25 1.8 SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|ch... 25 1.8 SPCC1223.13 |cbf12||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Ma... 25 2.4 SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 25 3.2 SPCC306.03c |cnd2||condensin subunit Cnd2|Schizosaccharomyces po... 25 3.2 SPAC688.06c |slx4||structure-specific endonuclease subunit |Schi... 25 3.2 SPAC26H5.09c |||GFO/IDH/MocA family oxidoreductase|Schizosacchar... 24 4.3 SPAC23C4.13 |bet1||SNARE Bet1|Schizosaccharomyces pombe|chr 1|||... 23 7.4 SPAC1687.04 |||conserved eukaryotic protein|Schizosaccharomyces ... 23 7.4 SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom... 23 7.4 SPAC222.15 |meu13|SPAC821.01|Tat binding protein 1|Schizosacchar... 23 9.8 SPBC11C11.01 ||SPBC17D1.08|RNA-binding protein|Schizosaccharomyc... 23 9.8 SPAC1F12.02c |p23fy||translationally controlled tumor protein ho... 23 9.8 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 23 9.8 SPAC323.07c |||MatE family transporter|Schizosaccharomyces pombe... 23 9.8 >SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces pombe|chr 3|||Manual Length = 238 Score = 27.1 bits (57), Expect = 0.60 Identities = 16/53 (30%), Positives = 27/53 (50%), Gaps = 2/53 (3%) Query: 43 YSKLANQTDSPNRFDIYDNDILSMQDKLLGNQNEQLQVISETVGSLKTVSKQI 95 Y L ++ + N I+D + + KLL N+NE L+ +SE + K K + Sbjct: 13 YDSLKSKISNFN--SIFDRFLQEERKKLLNNKNEYLRQLSEINEAQKKAEKSL 63 >SPBC1711.05 |||nucleocytoplasmic transport chaperone Srp40 |Schizosaccharomyces pombe|chr 2|||Manual Length = 451 Score = 26.6 bits (56), Expect = 0.80 Identities = 22/87 (25%), Positives = 35/87 (40%), Gaps = 2/87 (2%) Query: 6 SLNRNRDNDGTAREPLLGDDSPMNFGNNWTSTPKYTKYSKLA--NQTDSPNRFDIYDNDI 63 S + + D+D T+ S GN+ T T + S + N T S + D D Sbjct: 292 SESSSEDSDSTSSSSDSDSSSSSEDGNSNTDTTTSGEVSAQSSTNSTSSEESTSVKDEDS 351 Query: 64 LSMQDKLLGNQNEQLQVISETVGSLKT 90 + DK L ++E + + T S T Sbjct: 352 SKIHDKSLKRKHEDDESSTSTKSSRTT 378 >SPAPB1A10.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 529 Score = 26.6 bits (56), Expect = 0.80 Identities = 19/65 (29%), Positives = 26/65 (40%) Query: 3 SKMSLNRNRDNDGTAREPLLGDDSPMNFGNNWTSTPKYTKYSKLANQTDSPNRFDIYDND 62 SK S R A+ P L D+ N GN S+P SK DS + + Sbjct: 288 SKSSQQPVRTKPRIAQSPFLAQDAKENGGNVEVSSPLSFSASKSPAAVDSSTKTPTEQVN 347 Query: 63 ILSMQ 67 ++S Q Sbjct: 348 VVSKQ 352 >SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces pombe|chr 1|||Manual Length = 1679 Score = 25.8 bits (54), Expect = 1.4 Identities = 13/32 (40%), Positives = 20/32 (62%) Query: 57 DIYDNDILSMQDKLLGNQNEQLQVISETVGSL 88 ++YDND LS D LL ++ +++SE SL Sbjct: 535 ELYDNDKLSSIDALLIQDSKLREMVSEKCISL 566 >SPBC1347.05c |||DNAJ domain protein Scj1|Schizosaccharomyces pombe|chr 2|||Manual Length = 381 Score = 25.4 bits (53), Expect = 1.8 Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 3/40 (7%) Query: 57 DIYDNDILSMQDKLLGNQNEQLQVISETVGSLKTVSKQIG 96 D+Y + +S+++ LLGN ++Q + GS V + G Sbjct: 278 DLYRKETISVREALLGNWKRKIQKLD---GSFMEVKRSAG 314 >SPCC569.01c |||DUF1773 family protein 5|Schizosaccharomyces pombe|chr 3|||Manual Length = 323 Score = 25.4 bits (53), Expect = 1.8 Identities = 11/39 (28%), Positives = 23/39 (58%) Query: 47 ANQTDSPNRFDIYDNDILSMQDKLLGNQNEQLQVISETV 85 A Q + +RFD N++ SM+ ++ + E +++ ET+ Sbjct: 128 AMQNEMNSRFDAVQNEMTSMKGEMAEMKVEMVEMKRETI 166 >SPAC14C4.09 |agn1||glucan endo-1,3-alpha-glucosidase Agn1|Schizosaccharomyces pombe|chr 1|||Manual Length = 424 Score = 25.4 bits (53), Expect = 1.8 Identities = 18/62 (29%), Positives = 27/62 (43%), Gaps = 2/62 (3%) Query: 14 DGTAREPLLGDDSPMNFGNNWTSTPKYTKYSKLANQTDSPNRFDIYDNDILSMQDKLLGN 73 D +EPL P+ F +WTS + +A+ S N + D D+ D +G Sbjct: 144 DSAVKEPLASAGYPIYFVPSWTSLGQGALEESVADGFLSWNAWPTTDADMNDNDD--IGY 201 Query: 74 QN 75 QN Sbjct: 202 QN 203 >SPCP20C8.01c |||B13958 domain|Schizosaccharomyces pombe|chr 3|||Manual Length = 247 Score = 25.4 bits (53), Expect = 1.8 Identities = 11/39 (28%), Positives = 23/39 (58%) Query: 47 ANQTDSPNRFDIYDNDILSMQDKLLGNQNEQLQVISETV 85 A Q + +RFD N++ SM+ ++ + E +++ ET+ Sbjct: 128 AMQNEMNSRFDTVQNEMTSMKGEMAEMKVEMVEMKRETI 166 >SPBC36B7.07 |tlg1||SNARE Tgl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 225 Score = 25.4 bits (53), Expect = 1.8 Identities = 11/37 (29%), Positives = 23/37 (62%) Query: 67 QDKLLGNQNEQLQVISETVGSLKTVSKQIGLELDEQA 103 Q++L Q+ L + +T+G+++ + +G EL +QA Sbjct: 132 QEQLYQQQDVMLDGVYDTIGNIRGQAALMGEELGQQA 168 >SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 840 Score = 25.4 bits (53), Expect = 1.8 Identities = 15/50 (30%), Positives = 22/50 (44%), Gaps = 3/50 (6%) Query: 43 YSKLANQTDSPNRFDIYDNDILSMQDKLLGN---QNEQLQVISETVGSLK 89 Y+ A+ D P+RF+ +DN D N QN Q T+ +K Sbjct: 17 YNDGASNKDYPDRFNEFDNSQNDHNDYTQNNAQFQNAQTTTFGRTISRVK 66 >SPCC1223.13 |cbf12||CBF1/Su|Schizosaccharomyces pombe|chr 3|||Manual Length = 963 Score = 25.0 bits (52), Expect = 2.4 Identities = 20/82 (24%), Positives = 34/82 (41%), Gaps = 2/82 (2%) Query: 10 NRDNDGTAREPLLGDDSPMNFGNNWTSTPKYTKYSKLANQTDSPNRFDIYDNDILSMQDK 69 ++DN T P +P N + + YS L + + PN + ++IL Q Sbjct: 115 SQDNTQTYSTPYT-TMNPSNEMHPYPPATFENNYSVLPDHSSQPNAYSFTGSNILPTQSP 173 Query: 70 LLGNQNEQLQVISETVGSLKTV 91 L NQ + Q + + S T+ Sbjct: 174 SL-NQMQDYQNLQQNGSSNTTI 194 >SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces pombe|chr 2|||Manual Length = 603 Score = 24.6 bits (51), Expect = 3.2 Identities = 22/77 (28%), Positives = 29/77 (37%), Gaps = 3/77 (3%) Query: 9 RNRDNDGTAREPLLGDDSPMNFGNNWTSTPKYTKYSKLANQTDSPNRFDI-YDNDILSMQ 67 RN N ++ PL D SP N+ ++ S SPN F DN L Sbjct: 213 RNPGNISSSSAPLASDQSPTVSSNHIHASGNLALGSNSGLNPRSPNSFSSPLDNPALHTV 272 Query: 68 DKLLGNQNEQLQVISET 84 D N N L +S + Sbjct: 273 DST--NVNGSLSPLSNS 287 >SPCC306.03c |cnd2||condensin subunit Cnd2|Schizosaccharomyces pombe|chr 3|||Manual Length = 742 Score = 24.6 bits (51), Expect = 3.2 Identities = 15/45 (33%), Positives = 21/45 (46%), Gaps = 5/45 (11%) Query: 12 DNDGTAREPLLGDDSPMNFGNNWTSTPKYTK-----YSKLANQTD 51 D+D + PLL S FG+N T + K Y+K A + D Sbjct: 588 DSDSPKQTPLLTPPSSSGFGDNLLLTARLAKPDMLNYAKRAKKVD 632 >SPAC688.06c |slx4||structure-specific endonuclease subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 24.6 bits (51), Expect = 3.2 Identities = 13/45 (28%), Positives = 26/45 (57%) Query: 60 DNDILSMQDKLLGNQNEQLQVISETVGSLKTVSKQIGLELDEQAV 104 DN+ S+++KL E+L+ I+E+ G + SK +++ E + Sbjct: 274 DNEAKSLKEKLENFPVEKLRAIAESYGFKSSDSKATLIKIVESCL 318 >SPAC26H5.09c |||GFO/IDH/MocA family oxidoreductase|Schizosaccharomyces pombe|chr 1|||Manual Length = 369 Score = 24.2 bits (50), Expect = 4.3 Identities = 13/43 (30%), Positives = 22/43 (51%) Query: 40 YTKYSKLANQTDSPNRFDIYDNDILSMQDKLLGNQNEQLQVIS 82 Y + + A T+S R + + + D+LL + N +L VIS Sbjct: 33 YAAWERRATSTESKARAAFPNVKVYTKLDELLADSNIELVVIS 75 >SPAC23C4.13 |bet1||SNARE Bet1|Schizosaccharomyces pombe|chr 1|||Manual Length = 117 Score = 23.4 bits (48), Expect = 7.4 Identities = 11/42 (26%), Positives = 22/42 (52%) Query: 58 IYDNDILSMQDKLLGNQNEQLQVISETVGSLKTVSKQIGLEL 99 +Y++ D L +E++ ++ V SLK ++ IG E+ Sbjct: 18 LYESQSPQHLDSLENENDERISKLTGKVKSLKELTMNIGTEI 59 >SPAC1687.04 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 501 Score = 23.4 bits (48), Expect = 7.4 Identities = 11/33 (33%), Positives = 18/33 (54%) Query: 40 YTKYSKLANQTDSPNRFDIYDNDILSMQDKLLG 72 + Y++ A Q SP + +I IL +K+LG Sbjct: 214 FKDYTQSATQAPSPQQAEIIRPKILKYFEKVLG 246 >SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pombe|chr 1|||Manual Length = 1325 Score = 23.4 bits (48), Expect = 7.4 Identities = 11/26 (42%), Positives = 16/26 (61%) Query: 69 KLLGNQNEQLQVISETVGSLKTVSKQ 94 KL N NE+ + +ET G T++KQ Sbjct: 777 KLSENDNEKAEESNETKGFNTTIAKQ 802 >SPAC222.15 |meu13|SPAC821.01|Tat binding protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 216 Score = 23.0 bits (47), Expect = 9.8 Identities = 11/44 (25%), Positives = 23/44 (52%) Query: 56 FDIYDNDILSMQDKLLGNQNEQLQVISETVGSLKTVSKQIGLEL 99 F +D+ + + L +Q+Q + + V +KT+ K+ +EL Sbjct: 74 FVCLQDDLAAATPEELAEMEKQIQELKDEVSVVKTLYKEKCIEL 117 >SPBC11C11.01 ||SPBC17D1.08|RNA-binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 493 Score = 23.0 bits (47), Expect = 9.8 Identities = 11/32 (34%), Positives = 17/32 (53%) Query: 40 YTKYSKLANQTDSPNRFDIYDNDILSMQDKLL 71 Y K ++AN+ D I N+IL+M L+ Sbjct: 102 YEKNEEIANENDVDQGMFIQQNNILNMLVNLM 133 >SPAC1F12.02c |p23fy||translationally controlled tumor protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 168 Score = 23.0 bits (47), Expect = 9.8 Identities = 11/46 (23%), Positives = 21/46 (45%) Query: 40 YTKYSKLANQTDSPNRFDIYDNDILSMQDKLLGNQNEQLQVISETV 85 Y K K Q +P R +++ + + K+L N + I E++ Sbjct: 91 YMKAIKARLQESNPERVPVFEKNAIGFVKKILANFKDYDFYIGESM 136 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 23.0 bits (47), Expect = 9.8 Identities = 15/58 (25%), Positives = 26/58 (44%) Query: 44 SKLANQTDSPNRFDIYDNDILSMQDKLLGNQNEQLQVISETVGSLKTVSKQIGLELDE 101 +KL NQ D N+ D+L ++ L+ + E L + SL ++ LD+ Sbjct: 1393 NKLKNQLDHLNQEIRLKEDVLKEKESLIISLEESLSNQRQKESSLLDAKNELEHMLDD 1450 >SPAC323.07c |||MatE family transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 533 Score = 23.0 bits (47), Expect = 9.8 Identities = 12/43 (27%), Positives = 22/43 (51%) Query: 43 YSKLANQTDSPNRFDIYDNDILSMQDKLLGNQNEQLQVISETV 85 +SKL +++ + R D+D + +LL +N Q +E V Sbjct: 5 FSKLFSKSPTSGRVPSPDSDYSEEEQRLLAEENGYFQDSNEYV 47 Database: spombe Posted date: Oct 3, 2007 3:31 PM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.308 0.127 0.350 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 519,847 Number of Sequences: 5004 Number of extensions: 21923 Number of successful extensions: 57 Number of sequences better than 10.0: 23 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 35 Number of HSP's gapped (non-prelim): 25 length of query: 104 length of database: 2,362,478 effective HSP length: 63 effective length of query: 41 effective length of database: 2,047,226 effective search space: 83936266 effective search space used: 83936266 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (21.6 bits) S2: 47 (23.0 bits)
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