Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000579-TA|BGIBMGA000579-PA|IPR001194|DENN,
IPR005112|dDENN
(386 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g49040.2 68414.m05499 stomatal cytokinesis defective / SCD1 p... 67 2e-11
At1g49040.1 68414.m05498 stomatal cytokinesis defective / SCD1 p... 67 2e-11
At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein ... 60 3e-09
At2g20320.1 68415.m02373 DENN (AEX-3) domain-containing protein ... 42 5e-04
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 33 0.32
At3g02750.1 68416.m00267 protein phosphatase 2C family protein /... 30 2.3
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 2.3
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 2.3
At5g28400.1 68418.m03448 expressed protein 29 4.0
At5g28320.1 68418.m03438 expressed protein This is likely a pseu... 29 4.0
At5g47850.1 68418.m05912 protein kinase, putative contains simil... 29 5.3
At5g36250.1 68418.m04373 protein phosphatase 2C, putative / PP2C... 29 5.3
At2g36810.1 68415.m04514 expressed protein 29 5.3
At1g10760.1 68414.m01231 starch excess protein (SEX1) identical ... 29 5.3
At5g13110.1 68418.m01502 glucose-6-phosphate 1-dehydrogenase, pu... 29 6.9
At3g58760.1 68416.m06549 ankyrin protein kinase, putative simila... 29 6.9
At2g30220.1 68415.m03676 GDSL-motif lipase/hydrolase family prot... 28 9.2
At1g27880.1 68414.m03416 ATP-dependent DNA helicase, putative si... 28 9.2
>At1g49040.2 68414.m05499 stomatal cytokinesis defective / SCD1
protein (SCD1) contains Pfam PF02141: DENN (AEX-3)
domain; contains Pfam PF00400: WD domain, G-beta repeat
(8 copies); identical to stomatal cytokinesis defective
[Arabidopsis thaliana] GI:19743728; supporting cDNA
gi|19743727|gb|AY082605.1|; PMID 12874123
Length = 909
Score = 66.9 bits (156), Expect = 2e-11
Identities = 66/269 (24%), Positives = 124/269 (46%), Gaps = 46/269 (17%)
Query: 87 PMPGHTLKVIYNAGHSVFTCQCPDSNKLPSIPDNCNLTEYFSAVDTKCMAGLWAALLHER 146
P PG +V++ + + + + P + LP + +L +D + L+ ++L ER
Sbjct: 174 PTPGKD-RVLFAVENCLLSVEAPPEDSLPQA--DISLQPLVQCLDVDNLIKLFTSVLVER 230
Query: 147 RVAIVASKPSRLSACVQAANATLFPMSWQHIFIPILPKHLVDYMLAPMPFLIGVPRSV-M 205
R+ I ++K S L+ ++ ++P W ++IP+L VDY+ AP P+++G+ V
Sbjct: 231 RILIRSNKYSLLTLVSESICHLIYPFRWLQVYIPLLFFSGVDYIDAPTPYMMGLHSDVDT 290
Query: 206 ENVRMSEIGDVVILDVDANELRS-------PFNDLETLPPELISSLKK---ALSDKNALG 255
N+ M VV++D+D N++ + P + TL +++ L A+ G
Sbjct: 291 SNLAMD---GVVVVDLDINQITTSEEIPQIPEPEFSTLRNDILKLLHPNVVAIDQLKGFG 347
Query: 256 DAVSRA-------------------FLRALVCLIGGYRDAIKIENGQLITFNPEAFVKTR 296
++V + FL+ ++GGYR+ IEN F+ +AF+K R
Sbjct: 348 NSVEQCPKSLSKPWGEDHDLQLRVIFLKCFASILGGYRNF--IENK---VFSTDAFLKRR 402
Query: 297 KNM-----QPFLRKILQSQIFQQFIDERL 320
+P L + L S F +++ RL
Sbjct: 403 SRSTNQPPEPMLVQFLGSFAFLDYLERRL 431
>At1g49040.1 68414.m05498 stomatal cytokinesis defective / SCD1
protein (SCD1) contains Pfam PF02141: DENN (AEX-3)
domain; contains Pfam PF00400: WD domain, G-beta repeat
(8 copies); identical to stomatal cytokinesis defective
[Arabidopsis thaliana] GI:19743728; supporting cDNA
gi|19743727|gb|AY082605.1|; PMID 12874123
Length = 1187
Score = 66.9 bits (156), Expect = 2e-11
Identities = 66/269 (24%), Positives = 124/269 (46%), Gaps = 46/269 (17%)
Query: 87 PMPGHTLKVIYNAGHSVFTCQCPDSNKLPSIPDNCNLTEYFSAVDTKCMAGLWAALLHER 146
P PG +V++ + + + + P + LP + +L +D + L+ ++L ER
Sbjct: 174 PTPGKD-RVLFAVENCLLSVEAPPEDSLPQA--DISLQPLVQCLDVDNLIKLFTSVLVER 230
Query: 147 RVAIVASKPSRLSACVQAANATLFPMSWQHIFIPILPKHLVDYMLAPMPFLIGVPRSV-M 205
R+ I ++K S L+ ++ ++P W ++IP+L VDY+ AP P+++G+ V
Sbjct: 231 RILIRSNKYSLLTLVSESICHLIYPFRWLQVYIPLLFFSGVDYIDAPTPYMMGLHSDVDT 290
Query: 206 ENVRMSEIGDVVILDVDANELRS-------PFNDLETLPPELISSLKK---ALSDKNALG 255
N+ M VV++D+D N++ + P + TL +++ L A+ G
Sbjct: 291 SNLAMD---GVVVVDLDINQITTSEEIPQIPEPEFSTLRNDILKLLHPNVVAIDQLKGFG 347
Query: 256 DAVSRA-------------------FLRALVCLIGGYRDAIKIENGQLITFNPEAFVKTR 296
++V + FL+ ++GGYR+ IEN F+ +AF+K R
Sbjct: 348 NSVEQCPKSLSKPWGEDHDLQLRVIFLKCFASILGGYRNF--IENK---VFSTDAFLKRR 402
Query: 297 KNM-----QPFLRKILQSQIFQQFIDERL 320
+P L + L S F +++ RL
Sbjct: 403 SRSTNQPPEPMLVQFLGSFAFLDYLERRL 431
>At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein
contains Pfam domain PF02141: DENN (AEX-3) domain; this
cDNA may contain an anomalously spliced intron...will
require further examination.
Length = 765
Score = 59.7 bits (138), Expect = 3e-09
Identities = 26/90 (28%), Positives = 54/90 (60%), Gaps = 2/90 (2%)
Query: 140 AALLHERRVAIVASKPSRLSACVQAANATLFPMSWQHIFIPILPKHLVDYMLAPMPFLIG 199
A L E+++ V S L+A V + + P WQ + +P+LP +++++ AP+P+++G
Sbjct: 557 AGALLEKQIVFVCSNLGILAASVLSIIPVIRPFRWQSLLMPVLPDDMLEFLDAPVPYIVG 616
Query: 200 VPRSVMENVRMSEIGDVVILDVDANELRSP 229
V E S++ +V+++D+ N+++SP
Sbjct: 617 VKNKTSE--VQSKLTNVIVVDILKNQVKSP 644
>At2g20320.1 68415.m02373 DENN (AEX-3) domain-containing protein
contains Pfam domain PF02141: DENN (AEX-3) domain
Length = 976
Score = 42.3 bits (95), Expect = 5e-04
Identities = 17/51 (33%), Positives = 31/51 (60%)
Query: 150 IVASKPSRLSACVQAANATLFPMSWQHIFIPILPKHLVDYMLAPMPFLIGV 200
I++ + LSA V + + P WQ + +P+LP + D++ AP+PFL+ +
Sbjct: 814 ILSLETGVLSAIVLSLVPMIRPFQWQSLLLPVLPGRMFDFLEAPVPFLVKI 864
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 33.1 bits (72), Expect = 0.32
Identities = 18/53 (33%), Positives = 30/53 (56%), Gaps = 1/53 (1%)
Query: 14 ELFCEVSPGDHLKEPYVLFK-YPESYKNEEEIKSIPKFTFPCSLEKLLNCIAN 65
E+ ++ D + + LFK Y E+ K E++ +S + FPC L+ L NC+ N
Sbjct: 1125 EMGVKIFCADIIYHLFDLFKAYIENIKEEKKKESADEAVFPCVLQILPNCVFN 1177
>At3g02750.1 68416.m00267 protein phosphatase 2C family protein /
PP2C family protein similar to protein phosphatase-2C;
PP2C (GI:3643088) [Mesembryanthemum crystallinum];
contains Pfam PF00481 : Protein phosphatase 2C domain;
Length = 492
Score = 30.3 bits (65), Expect = 2.3
Identities = 15/24 (62%), Positives = 17/24 (70%)
Query: 137 GLWAALLHERRVAIVASKPSRLSA 160
G+W L +E VAIVAS PSR SA
Sbjct: 339 GIWDVLSNEDVVAIVASAPSRSSA 362
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 30.3 bits (65), Expect = 2.3
Identities = 13/32 (40%), Positives = 20/32 (62%)
Query: 34 YPESYKNEEEIKSIPKFTFPCSLEKLLNCIAN 65
Y E+ K E++ +S + FPC L+ L NC+ N
Sbjct: 482 YIENIKEEKKKESAGEAVFPCVLQILPNCVFN 513
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 30.3 bits (65), Expect = 2.3
Identities = 13/32 (40%), Positives = 20/32 (62%)
Query: 34 YPESYKNEEEIKSIPKFTFPCSLEKLLNCIAN 65
Y E+ K E++ +S + FPC L+ L NC+ N
Sbjct: 1070 YIENIKEEKKKESAGEAVFPCVLQILPNCVFN 1101
>At5g28400.1 68418.m03448 expressed protein
Length = 996
Score = 29.5 bits (63), Expect = 4.0
Identities = 21/74 (28%), Positives = 42/74 (56%), Gaps = 5/74 (6%)
Query: 179 IPILPKHLVDYMLAPMPFLIGVPRSVMENVRMSEIGDVVILDVDANELRSPFNDLETLPP 238
+P++P H + + + G+ + + V + +G+V ++VD +E+ S + L+ L
Sbjct: 882 VPLVPTHGI--IQSSEAEYCGICSNPIFTVYLDSVGNVAKVEVDEDEVLSRRSGLDDL-- 937
Query: 239 ELISS-LKKALSDK 251
EL+SS L+K L D+
Sbjct: 938 ELVSSRLQKVLEDQ 951
>At5g28320.1 68418.m03438 expressed protein This is likely a
pseudogene.
Length = 967
Score = 29.5 bits (63), Expect = 4.0
Identities = 21/74 (28%), Positives = 42/74 (56%), Gaps = 5/74 (6%)
Query: 179 IPILPKHLVDYMLAPMPFLIGVPRSVMENVRMSEIGDVVILDVDANELRSPFNDLETLPP 238
+P++P H + + + G+ + + V + +G+V ++VD +E+ S + L+ L
Sbjct: 853 VPLVPTHGI--IQSSEAEYCGICSNPIFTVYLDSVGNVAKVEVDEDEVLSRRSGLDDL-- 908
Query: 239 ELISS-LKKALSDK 251
EL+SS L+K L D+
Sbjct: 909 ELVSSRLQKVLEDQ 922
>At5g47850.1 68418.m05912 protein kinase, putative contains
similarity to cytokinin-regulated kinase 1 [Nicotiana
tabacum] gi|10998537|gb|AAG25966; contains protein
kinase domain, Pfam:PF00069
Length = 751
Score = 29.1 bits (62), Expect = 5.3
Identities = 15/45 (33%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Query: 73 DQLHSFLEACRIRPPMPGHTLKVIYNAGHSVFTCQCPDSNKLPSI 117
D+ H L+ RI PP P + ++ + + G+ C P S K PS+
Sbjct: 680 DEAHRILDQ-RIPPPTP-YEIEAVAHVGYLAAECLMPCSRKRPSM 722
>At5g36250.1 68418.m04373 protein phosphatase 2C, putative / PP2C,
putative
Length = 448
Score = 29.1 bits (62), Expect = 5.3
Identities = 15/39 (38%), Positives = 20/39 (51%)
Query: 137 GLWAALLHERRVAIVASKPSRLSACVQAANATLFPMSWQ 175
G+W AL +E V IVA P+R SA A + W+
Sbjct: 330 GIWDALTNEEVVKIVAKAPTRSSAGRALVEAAVRNWRWK 368
>At2g36810.1 68415.m04514 expressed protein
Length = 1071
Score = 29.1 bits (62), Expect = 5.3
Identities = 26/93 (27%), Positives = 42/93 (45%), Gaps = 4/93 (4%)
Query: 253 ALGDAVSRAFLRALVCLIGGYRDAIKIENGQLITFNPEAFVKTRKNMQPFLRKILQSQIF 312
A G+ VS LRA VC G + I +L FN + ++K + + PFL K++ +++
Sbjct: 208 AKGELVSPTQLRA-VCGKGLLLLTVTIPEMELSDFNAKEYMKLQYILWPFLLKMIIPKVY 266
Query: 313 Q---QFIDERLDLLNSGRGFNDEFEVECNAYAD 342
+ + L R +EC A AD
Sbjct: 267 TGAVASVCRCITELCRRRSSTTPMLIECKARAD 299
>At1g10760.1 68414.m01231 starch excess protein (SEX1) identical to
SEX1 [Arabidopsis thaliana] GI:12044358; supporting cDNA
gi|12044357|gb|AF312027.1|AF312027
Length = 1399
Score = 29.1 bits (62), Expect = 5.3
Identities = 18/41 (43%), Positives = 26/41 (63%), Gaps = 2/41 (4%)
Query: 215 DVVILDVDANELRSPFND-LETLPPELISSLKKALSDKNAL 254
DVV +V+ +EL SP +D LE PP IS +KK + + A+
Sbjct: 1044 DVVYKEVNDSELSSPSSDNLEDAPPS-ISLVKKQFAGRYAI 1083
>At5g13110.1 68418.m01502 glucose-6-phosphate 1-dehydrogenase,
putative / G6PD, putative similar to SP|Q43839
Glucose-6-phosphate 1-dehydrogenase, chloroplast
precursor (EC 1.1.1.49) (G6PD) {Solanum tuberosum};
contains Pfam profiles PF02781: Glucose-6-phosphate
dehydrogenase C-terminal, PF00479: Glucose-6-phosphate
dehydrogenase NAD binding domain
Length = 596
Score = 28.7 bits (61), Expect = 6.9
Identities = 11/38 (28%), Positives = 20/38 (52%)
Query: 25 LKEPYVLFKYPESYKNEEEIKSIPKFTFPCSLEKLLNC 62
L E + +F Y S + E++++ T C ++K NC
Sbjct: 139 LPEHFTIFGYSRSKMTDVELRNMVSKTLTCRIDKRANC 176
>At3g58760.1 68416.m06549 ankyrin protein kinase, putative similar
to ankyrin-kinase [Medicago truncatula]
gi|18700701|gb|AAL78674
Length = 471
Score = 28.7 bits (61), Expect = 6.9
Identities = 18/82 (21%), Positives = 36/82 (43%), Gaps = 2/82 (2%)
Query: 35 PESYKNEEEIKSIPKFTFPCSLEKLLNCIANIINSPERDQLHSFLEACRIRPPMPGHTLK 94
PE Y+NEE + F+F L++++ +R+ +++E RPP T
Sbjct: 337 PEVYRNEEYDTKVDVFSFALILQEMIEGCEPFHEIEDREVPKAYIE--DERPPFNAPTKS 394
Query: 95 VIYNAGHSVFTCQCPDSNKLPS 116
+ + C +++K P+
Sbjct: 395 YPFGLQELIQDCWDKEASKRPT 416
>At2g30220.1 68415.m03676 GDSL-motif lipase/hydrolase family protein
similar to family II lipases EXL3 GI:15054386, EXL1
GI:15054382, EXL2 GI:15054384 from [Arabidopsis
thaliana]; contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase
Length = 358
Score = 28.3 bits (60), Expect = 9.2
Identities = 17/62 (27%), Positives = 31/62 (50%), Gaps = 4/62 (6%)
Query: 278 KIENGQLITFNPEAFVKTRKNMQPFLRKILQSQIFQQFIDERLDLLNSGRGFNDEFEVEC 337
+ NG+LI+ + T+ N++ F+ LQ I Q I + ++G G++DE +
Sbjct: 74 RFSNGKLIS----DVISTKLNIKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSS 129
Query: 338 NA 339
A
Sbjct: 130 KA 131
>At1g27880.1 68414.m03416 ATP-dependent DNA helicase, putative
similar to SP|O94761 ATP-dependent DNA helicase Q4
(RecQ4) {Homo sapiens}; contains Pfam profiles PF00270:
DEAD/DEAH box helicase, PF00271: Helicase conserved
C-terminal domain
Length = 911
Score = 28.3 bits (60), Expect = 9.2
Identities = 12/40 (30%), Positives = 20/40 (50%)
Query: 340 YADKLNTGSGQKLKQQYRDWAKTVKKEGGAFFKTVKDKVL 379
Y K G G+ L ++ D K ++ E F +V+D +L
Sbjct: 207 YKKKEADGDGESLLEEESDLQKQIEDEANGFISSVEDAIL 246
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.322 0.138 0.416
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,611,001
Number of Sequences: 28952
Number of extensions: 421184
Number of successful extensions: 1002
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 986
Number of HSP's gapped (non-prelim): 23
length of query: 386
length of database: 12,070,560
effective HSP length: 82
effective length of query: 304
effective length of database: 9,696,496
effective search space: 2947734784
effective search space used: 2947734784
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 60 (28.3 bits)
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