Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA000578-TA|BGIBMGA000578-PA|IPR007114|Major facilitator
superfamily, IPR011701|Major facilitator superfamily MFS_1
(618 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41660.1 68415.m05147 expressed protein contains Pfam profile... 36 0.080
At1g76430.1 68414.m08885 phosphate transporter family protein si... 34 0.32
At2g16970.1 68415.m01955 expressed protein ; expression support... 33 0.56
At5g48300.1 68418.m05966 glucose-1-phosphate adenylyltransferase... 32 0.98
At5g13390.1 68418.m01542 expressed protein 32 0.98
At2g39210.1 68415.m04816 nodulin family protein similar to nodul... 31 2.3
At1g08930.1 68414.m00994 early-responsive to dehydration stress ... 31 3.0
At3g46980.2 68416.m05102 transporter-related low similarity to b... 30 5.2
At3g46980.1 68416.m05101 transporter-related low similarity to b... 30 5.2
At3g13050.1 68416.m01626 transporter-related low similarity to a... 30 5.2
At4g16990.2 68417.m02562 disease resistance protein (TIR-NBS cla... 29 6.9
At3g27170.1 68416.m03398 chloride channel protein (CLC-b) identi... 29 6.9
At1g52580.1 68414.m05936 rhomboid family protein contains PFAM d... 29 6.9
At5g44770.1 68418.m05487 DC1 domain-containing protein contains ... 29 9.1
At1g75800.1 68414.m08805 pathogenesis-related thaumatin family p... 29 9.1
At1g13210.1 68414.m01532 haloacid dehalogenase-like hydrolase fa... 29 9.1
At1g02890.1 68414.m00256 AAA-type ATPase family protein contains... 29 9.1
>At2g41660.1 68415.m05147 expressed protein contains Pfam profile
PF04759: Protein of unknown function, DUF617
Length = 297
Score = 35.9 bits (79), Expect = 0.080
Identities = 15/47 (31%), Positives = 28/47 (59%)
Query: 535 SIVSPPGTETTIQGLVGAVFEGVGASLGSFIGGQLYHHYQGWITFRI 581
S+ SPP + +++ + ++ G +SLG + G LY H +G +TF +
Sbjct: 99 SLPSPPSSSSSVSNQLISLVTGGSSSLGRRVTGTLYGHKRGHVTFSV 145
>At1g76430.1 68414.m08885 phosphate transporter family protein
similar to phosphate transporters from [Catharanthus
roseus] GI:2208908 and [Nicotiana tabacum] GI:12641853,
transmembrane protein [Solanum tuberosum] GI:17065936;
contains Pfam profile: PF00083 major facilitator
superfamily protein
Length = 532
Score = 33.9 bits (74), Expect = 0.32
Identities = 39/184 (21%), Positives = 79/184 (42%), Gaps = 20/184 (10%)
Query: 448 DSVMYIKTLQGLVSAIQTFFGEIPFLFVSGYILKKVGHVKMMSLVLFAFGVRFILYSFLT 507
DS + L +V+A T IP + + Y + K+G VK+ + F V +++
Sbjct: 336 DSAFEVAKLAAIVAACST----IPGYWFTVYFIDKIGRVKIQMMGFFLMAVVYLVAGIPY 391
Query: 508 NAWWILPIEMLQG--ITFGM------FYPTMTSY--ASIVSPPGTETTIQGLVGAVFEGV 557
+ +W + +G + +G+ F P T++ + + P +T G+ GA +
Sbjct: 392 SWYWSKHEKTNKGFMVLYGLIFFFSNFGPNTTTFIIPAELFPARFRSTCHGISGAAGK-F 450
Query: 558 GASLGS--FIGGQLYHHYQGWITFRIYGIGSLV---CCALHLTVQYVFKDKIQRVGLSQG 612
GA +G+ F+ +H G+ + I L+ C + V Y+F + L +
Sbjct: 451 GAIVGTVGFLWATRHHEEDGFPDVKRVRIAFLILGGVCIAGMIVTYLFTRETMGRSLEEN 510
Query: 613 KKDM 616
+ ++
Sbjct: 511 EDEI 514
>At2g16970.1 68415.m01955 expressed protein ; expression supported
by MPSS
Length = 414
Score = 33.1 bits (72), Expect = 0.56
Identities = 26/97 (26%), Positives = 41/97 (42%), Gaps = 3/97 (3%)
Query: 465 TFFGEIPFLFVSGYILKKVGHVKMMSLVLFAFGVRFILYSFLTNAWWILPIEMLQGITFG 524
T G I LF+ ++ +G +++S L V S +AW +L +T
Sbjct: 257 TIIGSISQLFILPKLVSAIGERRVLSTGLLMDSVNAACLSVSWSAWVPYATTVLVPVTM- 315
Query: 525 MFYPTMTSYASIVSPPGTETTIQGLVGAV--FEGVGA 559
P++ AS PG + +QG + V F GV A
Sbjct: 316 FVMPSVCGIASRQVGPGEQGKVQGCISGVKSFSGVVA 352
>At5g48300.1 68418.m05966 glucose-1-phosphate adenylyltransferase
small subunit 1 (APS1) / ADP-glucose pyrophosphorylase
(ADG1) identical to SP|P55228
Length = 520
Score = 32.3 bits (70), Expect = 0.98
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Query: 193 PRCRIGQNYVDVNEPCSLNCTNENLSSVIGREKANMTCVDRHLNYRLCTN 242
P +G NY ++ P S NC N N+S + + N ++RHL+ +N
Sbjct: 113 PAVPLGANYRLIDIPVS-NCLNSNISKIYVLTQFNSASLNRHLSRAYASN 161
>At5g13390.1 68418.m01542 expressed protein
Length = 1123
Score = 32.3 bits (70), Expect = 0.98
Identities = 16/40 (40%), Positives = 23/40 (57%)
Query: 55 YYFRFKLQKSIFILFQFVTIISFSAIYFVPHNRPKINVEL 94
Y+ RF +KS+ F FV + S I+FV HN +N+ L
Sbjct: 418 YFARFFTKKSVPSYFAFVALGSLMVIWFVMHNYWDLNIWL 457
>At2g39210.1 68415.m04816 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 601
Score = 31.1 bits (67), Expect = 2.3
Identities = 24/94 (25%), Positives = 43/94 (45%), Gaps = 4/94 (4%)
Query: 452 YIKTLQGLVSAIQTFFGEIP--FLFVSGYILKKVGHVKMMSLVLFAFGVRFILYSFLTNA 509
Y + + G+VS I + P + +L GH+ + V V ++ F A
Sbjct: 393 YGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFNVPGGLYVASVIIGFCFGA 452
Query: 510 WWILPIEMLQGITFGM-FYPTMTSYASIVSPPGT 542
W L ++ I FG+ +Y T+ ++ S+ SP G+
Sbjct: 453 QWPLLFAIISEI-FGLKYYSTLYNFGSVASPIGS 485
>At1g08930.1 68414.m00994 early-responsive to dehydration stress
protein (ERD6) / sugar transporter family protein
identical to ERD6 protein {Arabidopsis thaliana}
GI:3123712; contains Pfam profile PF00083: major
facilitator superfamily protein; contains TIGRfam
TIGR00879: Sugar transporter
Length = 496
Score = 30.7 bits (66), Expect = 3.0
Identities = 41/185 (22%), Positives = 71/185 (38%), Gaps = 22/185 (11%)
Query: 412 LPTFVFMMWTVAVGLCTGLLWNFLFWLLEDVSRLSCDSVMY--IKTLQGLVSAIQTFFGE 469
L TFV + + G G + +D+S + M+ I TL GL+ A+
Sbjct: 60 LSTFVAVSGSFCTGCGVGFSSGAQAGITKDLSLSVAEYSMFGSILTLGGLIGAV------ 113
Query: 470 IPFLFVSGYILKKVGHVKMMSLVLFAFGVRFILYSFLTNAWWILPIEMLQGITFGMFYPT 529
SG + +G + M F ++ + NA W+ +L GI G+F
Sbjct: 114 -----FSGKVADVLGRKRTMLFCEFFCITGWLCVALAQNAMWLDCGRLLLGIGVGIFSYV 168
Query: 530 MTSYASIVSPPGTETTIQGLVGAVFEGVGASLGSFIGGQLYHHYQGWITFRIYGIGSLVC 589
+ Y + ++P + + + G SL IG +I +R+ + LV
Sbjct: 169 IPVYIAEIAPKHVRGSFV-FANQLMQNCGISLFFIIG--------NFIPWRLLTVVGLVP 219
Query: 590 CALHL 594
C H+
Sbjct: 220 CVFHV 224
>At3g46980.2 68416.m05102 transporter-related low similarity to
brain specific Na+-dependent inorganic phosphate
cotransporter from [Rattus norvegicus] GI:507415, [Homo
sapiens] GI:7328925, vesicular glutamate transporter 3
from [Rattus norvegicus] GI:21685382; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 469
Score = 29.9 bits (64), Expect = 5.2
Identities = 33/159 (20%), Positives = 69/159 (43%), Gaps = 12/159 (7%)
Query: 464 QTFFGEIPFLFVSGYILKKVGHVKMMSL----VLFAFGVR-FILYSFLT------NAWWI 512
++F G + F+ GY++ + ++ V+ A+GV + L +FLT + W +
Sbjct: 147 KSFSGIVQSSFLWGYLISPIAGGTLVDRYGGKVVMAWGVALWSLATFLTPWAADSSLWAL 206
Query: 513 LPIEMLQGITFGMFYPTMTSYASIVSPPGTETTIQGLVGAVFEGVGASLGSFIGGQLYHH 572
L + G+ G+ P M + + PP + G+ A F+ +G +G + L
Sbjct: 207 LAARAMVGVAEGVALPCMNNMVARWFPPTERSRAVGIAMAGFQ-LGNVVGLMLSPILMSQ 265
Query: 573 YQGWITFRIYGIGSLVCCALHLTVQYVFKDKIQRVGLSQ 611
+ F I+G+ + + L+ D+ ++ S+
Sbjct: 266 GGIYGPFVIFGLSGFLWLLVWLSATSSAPDRHPQITKSE 304
>At3g46980.1 68416.m05101 transporter-related low similarity to
brain specific Na+-dependent inorganic phosphate
cotransporter from [Rattus norvegicus] GI:507415, [Homo
sapiens] GI:7328925, vesicular glutamate transporter 3
from [Rattus norvegicus] GI:21685382; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 533
Score = 29.9 bits (64), Expect = 5.2
Identities = 33/159 (20%), Positives = 69/159 (43%), Gaps = 12/159 (7%)
Query: 464 QTFFGEIPFLFVSGYILKKVGHVKMMSL----VLFAFGVR-FILYSFLT------NAWWI 512
++F G + F+ GY++ + ++ V+ A+GV + L +FLT + W +
Sbjct: 147 KSFSGIVQSSFLWGYLISPIAGGTLVDRYGGKVVMAWGVALWSLATFLTPWAADSSLWAL 206
Query: 513 LPIEMLQGITFGMFYPTMTSYASIVSPPGTETTIQGLVGAVFEGVGASLGSFIGGQLYHH 572
L + G+ G+ P M + + PP + G+ A F+ +G +G + L
Sbjct: 207 LAARAMVGVAEGVALPCMNNMVARWFPPTERSRAVGIAMAGFQ-LGNVVGLMLSPILMSQ 265
Query: 573 YQGWITFRIYGIGSLVCCALHLTVQYVFKDKIQRVGLSQ 611
+ F I+G+ + + L+ D+ ++ S+
Sbjct: 266 GGIYGPFVIFGLSGFLWLLVWLSATSSAPDRHPQITKSE 304
>At3g13050.1 68416.m01626 transporter-related low similarity to
apical organic cation transporter [Sus scrofa]
GI:2062135, SP|Q02563 Synaptic vesicle protein 2 (SV2)
{Rattus norvegicus}; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 500
Score = 29.9 bits (64), Expect = 5.2
Identities = 29/123 (23%), Positives = 51/123 (41%), Gaps = 3/123 (2%)
Query: 467 FGEIPFLFVSGYILKKVGHVKMMSLVLFAFGVRFILYSFLTNAWWILPIEMLQG-ITFGM 525
F E P L +S ++ ++G M+ +LF + F+L + +I + + G I
Sbjct: 348 FAEFPGLLISAAMVDRLGRKASMASMLFTCCI-FLLPLLSHQSPFITTVLLFGGRICISA 406
Query: 526 FYPTMTSYASIVSPPGTETTIQGLVGAVFEGVGASLGSFIGGQLYHHYQGWITFRIYGIG 585
+ + YA + P TT G VG+ +G L + L H I ++ +
Sbjct: 407 AFTVVYIYAPEIYPTAVRTTGVG-VGSSVGRIGGILCPLVAVGLVHGCHQTIAVLLFEVV 465
Query: 586 SLV 588
LV
Sbjct: 466 ILV 468
>At4g16990.2 68417.m02562 disease resistance protein (TIR-NBS
class), putative domain signature TIR-NBS exists,
suggestive of a disease resistance protein.
Length = 796
Score = 29.5 bits (63), Expect = 6.9
Identities = 30/127 (23%), Positives = 53/127 (41%), Gaps = 13/127 (10%)
Query: 151 PQYCYSNTDRIRYTSHVTNVTLKDDC--AYFSANSV------ILDGLKFTPRCRIGQNYV 202
P+Y S++ R+ H ++C Y + N++ I G KF + ++ +
Sbjct: 562 PKYVESSSRRVTQVHHA-KTWWPENCEKVYENHNNIYGIDRSIDGGDKFEGKSKVSDGGL 620
Query: 203 DVNEPCSLNCTNENLSSVIGREKANMTC--VDRHL--NYRLCTNSSLADLTKQNIESDCQ 258
D + S+ + N I + N C V L NY +C+ + L D+ N +S +
Sbjct: 621 DGKDQGSMYGQSSNSELQINMDADNRRCEPVSEMLFKNYNVCSPNGLTDVNCSNPQSQRK 680
Query: 259 ADCVLDK 265
D L K
Sbjct: 681 LDASLKK 687
>At3g27170.1 68416.m03398 chloride channel protein (CLC-b) identical
to CLC-b chloride channel protein GB:CAA96058 from
[Arabidopsis thaliana] (J. Biol. Chem. 271 (52),
33632-33638 (1996))
Length = 780
Score = 29.5 bits (63), Expect = 6.9
Identities = 16/56 (28%), Positives = 30/56 (53%), Gaps = 2/56 (3%)
Query: 415 FVFMMWTVA--VGLCTGLLWNFLFWLLEDVSRLSCDSVMYIKTLQGLVSAIQTFFG 468
+VF+ WT+A VGL TGL+ + +E+++ +V + T + V+ + G
Sbjct: 81 YVFLKWTLACLVGLFTGLIATLINLAVENIAGYKLLAVGHFLTQERYVTGLMVLVG 136
>At1g52580.1 68414.m05936 rhomboid family protein contains PFAM
domain PF01694, Rhomboid family
Length = 309
Score = 29.5 bits (63), Expect = 6.9
Identities = 15/59 (25%), Positives = 29/59 (49%)
Query: 381 MGDVIVSCFLKTDSTKMSLNILGDVGTLLTSLPTFVFMMWTVAVGLCTGLLWNFLFWLL 439
+G +VSC + ++S+ G + LL ++ + + WT+ CT L+ L +L
Sbjct: 154 LGGSLVSCLTDSQGERVSVGASGALFGLLGAMLSELITNWTIYENKCTALMTLILIIVL 212
>At5g44770.1 68418.m05487 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 541
Score = 29.1 bits (62), Expect = 9.1
Identities = 21/82 (25%), Positives = 35/82 (42%), Gaps = 4/82 (4%)
Query: 112 SLDCKIQATGDLNVTSTCKMECNMGNPRMWETVCEYWHLPQYCYSNTDRIRYTSHVTNVT 171
+L K++ D + S C N +W +CE P CY D+ + H+ N
Sbjct: 421 TLPKKVKHRCDDHFLSLCHDAGNSSGD-LWCDICETKTDPSVCYYTCDKCGVSLHI-NCV 478
Query: 172 LKDDCAYFSANSVILDGLKFTP 193
L D Y++ ++ GL+ P
Sbjct: 479 LGD--LYYTKVGLVYPGLEVLP 498
>At1g75800.1 68414.m08805 pathogenesis-related thaumatin family
protein similar to receptor serine/threonine kinase PR5K
[Arabidopsis thaliana] GI:1235680; contains Pfam
profile: PF00314 Thaumatin family
Length = 330
Score = 29.1 bits (62), Expect = 9.1
Identities = 29/113 (25%), Positives = 43/113 (38%), Gaps = 5/113 (4%)
Query: 91 NVELNCGPAGTVLTSCYKDSPSLDCKIQATGDLNVTSTC-KMECNMGNPRMWETVCEYWH 149
NV + P G +C +D +L VTS + + +MG ++ CE +
Sbjct: 135 NVPMLVAPQGGSGLNCSSTGCVVDLNGSCPSELKVTSLDGRGKQSMG----CKSACEAFR 190
Query: 150 LPQYCYSNTDRIRYTSHVTNVTLKDDCAYFSANSVILDGLKFTPRCRIGQNYV 202
P+YC S T ++ +L A A S D T C NYV
Sbjct: 191 TPEYCCSGAHGTPDTCKPSSYSLMFKTACPRAYSYAYDDQSSTFTCAESPNYV 243
>At1g13210.1 68414.m01532 haloacid dehalogenase-like hydrolase family
protein similar to Potential phospholipid-transporting
ATPase (EC 3.6.3.1) (Chromaffin granule ATPase) from
{Homo sapiens} SP|Q9Y2Q0, {Mus musculus} SP|P98200, {Bos
taurus} SP|Q29449; contains InterPro accession IPR005834:
Haloacid dehalogenase-like hydrolase; ESTs gb|T45045 and
gb|AA394473 come from this gene
Length = 1203
Score = 29.1 bits (62), Expect = 9.1
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Query: 341 LWGSVG-WGIFSFLTGFLIDTFSDGAYKDYTVA 372
+WGSV W IF + G + +FS AYK + A
Sbjct: 1076 IWGSVAFWYIFLMIYGAITPSFSTDAYKVFIEA 1108
>At1g02890.1 68414.m00256 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family; similar to
mitochondrial sorting protein 1 (MSP1) (TAT-binding
homolog 4) (Swiss-Prot:P28737) [Saccharomyces
cerevisiae]
Length = 1252
Score = 29.1 bits (62), Expect = 9.1
Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Query: 528 PTMTSYASIVSPP-GTETTIQGLVGAVFEGVGAS 560
P+ +S AS+ +PP G T QG V FEG G+S
Sbjct: 614 PSTSSLASLRAPPRGPATGFQGKVLLAFEGNGSS 647
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.326 0.140 0.448
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,944,903
Number of Sequences: 28952
Number of extensions: 646070
Number of successful extensions: 1780
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1773
Number of HSP's gapped (non-prelim): 19
length of query: 618
length of database: 12,070,560
effective HSP length: 85
effective length of query: 533
effective length of database: 9,609,640
effective search space: 5121938120
effective search space used: 5121938120
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.6 bits)
S2: 62 (29.1 bits)
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